Standard name
Description Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.08 0.05 0 0 0.05 0.05 0 0
Bud 0 0 0.06 0.05 0.06 0.06 0.06 0.08 0.11 0.09 0.11 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.08 0.08 0 0.08 0 0 0 0 0 0.05 0 0 0.06 0.08 0.07 0.06 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.06 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.06 0 0.19 0.05 0 0.06 0 0 0 0 0.06 0.14 0.31 0.3 0.15 0.21 0.23 0.19 0.19 0.22 0.16 0.15
Golgi 0 0.05 0 0.07 0 0 0 0 0 0 0 0 0.25 0.33 0.27 0.41 0.55 0.43 0.5 0.28 0.49 0.38 0.52 0.56
Mitochondria 0.96 0.88 0.84 0.9 0.75 0.9 0.89 0.84 0.91 0.89 0.88 0.87 0.73 0.47 0.27 0.16 0.1 0.09 0.17 0.33 0.13 0.18 0.16 0.14
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.06 0.05 0 0.07 0.06 0.09 0.06 0.09 0 0 0.15 0 0 0 0 0 0.1 0.11 0.1 0.09
SpindlePole 0 0 0 0 0 0 0 0.06 0 0.05 0 0.08 0 0 0.08 0.09 0.06 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0 0 0 0.05 0 0 0 0 0 0 0 0.06 0.07 0.08 0.09 0.09 0.11 0 0.09 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 12 3 2 1 0 0 5 3 0 2 4 2 7 7 9 9 6 1 7 11 5 10 10
Bud 1 2 11 11 19 39 36 53 52 47 53 42 1 3 3 4 4 3 0 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 2 0 1 8 8 9 19 15 5 15 0 0 0 0 0 0
Cell Periphery 0 1 1 1 1 3 4 0 3 3 0 0 0 0 1 2 1 1 0 0 0 0 0 0
Cytoplasm 0 21 15 9 25 23 17 21 19 14 23 13 3 10 13 8 7 11 0 1 0 0 0 1
Endoplasmic Reticulum 2 17 0 2 1 3 5 6 3 2 5 4 4 5 3 4 4 4 0 2 1 0 3 1
Endosome 0 2 12 6 64 31 17 39 8 14 8 20 4 23 47 35 16 23 21 58 38 22 56 48
Golgi 2 13 8 15 9 0 1 2 0 1 0 0 18 53 42 48 61 47 45 86 100 38 182 179
Mitochondria 46 245 168 185 251 543 509 530 444 488 419 423 52 75 41 18 11 10 15 101 27 18 55 45
Nucleus 0 3 1 0 2 1 4 5 1 2 4 1 0 1 2 0 1 0 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 1 3 2 1 2 4 2 0 0 1 1 2 0 0 0 0 0 1 0
Nucleolus 2 0 2 2 1 10 18 28 15 17 21 9 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1 4 4 21 31 22 47 29 47 29 43 2 3 23 4 2 3 2 12 20 11 35 27
SpindlePole 0 2 3 4 11 26 25 39 13 25 17 40 1 5 12 10 7 8 1 4 0 0 1 0
Vac/Vac Membrane 4 7 4 7 18 14 5 6 4 5 6 5 4 11 12 10 10 12 1 26 1 2 3 3
Unique Cell Count 48 277 200 205 333 606 570 631 488 550 474 488 71 159 154 116 110 110 92 309 206 101 353 321
Labelled Cell Count 57 327 234 248 425 733 674 792 614 682 596 621 91 197 207 153 135 128 92 309 206 101 353 321


Early Golgi, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 5.6 6.3 6.1 6.3 4.8 4.9 5.4 4.8 4.8 4.8 4.7 7.6 7.6 7.8 8.3 8.1 8.1 5.8 7.3 7.2
Std Deviation (1e-4) 1.1 0.8 1.7 1.5 2.0 1.3 1.5 1.6 1.3 1.1 1.6 1.2 1.2 1.0 1.2 1.5 1.4 1.2 0.7 1.0 1.1
Intensity Change (Log2) -0.04 0.0 -0.38 -0.37 -0.23 -0.39 -0.39 -0.39 -0.43 0.27 0.27 0.31 0.39 0.37 0.36 -0.11 0.21 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -0.1 0.1 0 0 1.3 0 0 0 0 0 -1.8 -1.7 -0.8 -0.7 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.3 0 -2.1 -2.8 -2.5 -2.0 -3.1 -1.4 -2.9 0 -0.4 0.3 -0.2 -0.4 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -1.5 4.2 0 0 0.1 0 0 0 0 0 2.7 6.1 5.8 2.5 4.0
Golgi 1.4 -0.8 0 0 0 0 0 0 0 5.2 7.4 6.2 8.4 10.4 8.5
Mitochondria 1.9 -2.4 0 0 0 0 0 0 0 -2.0 -7.4 -10.9 -11.9 -12.6 -12.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 2.3 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 2.3 0 0 2.8 0 0 0 0 0 0 4.5 0 0 0
SpindlePole 0 1.3 0 0 2.6 0 0 0 0 0 0 2.9 0 0 0
Vacuole 0.9 1.9 0 0 0 0 0 0 0 0 2.3 2.6 2.8 0 3.4