Standard name
Human Ortholog
Description S-phase checkpoint protein required for DNA replication; couples DNA helicase and polymerase; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; defines a novel S-phase checkpoint with Hog1p that coordinates DNA replication and transcription upon osmostress; protects uncapped telomeres; Dia2p-dependent degradation mediates checkpoint recovery; mammalian claspin homolog

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.05 0 0 0 0 0 0 0.13 0.18 0.17 0.09 0.08 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.09 0.21 0.42 0.16 0.54 0.51 0.46 0.71 0.71 0.74 0.08 0 0.19 0 0 0.05 0 0 0 0 0 0
Nucleus 0.94 0.9 0.9 0.83 0.86 0.76 0.78 0.81 0.75 0.71 0.66 0.84 0.79 0.72 0.83 0.83 0.82 0.83 0.84 0.81 0.82 0.66 0.43
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.14
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.05 0.05 0.09 0.1 0.1 0.18 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1
Bud 0 0 0 0 0 3 3 2 4 8 4 0 1 1 0 0 0 4 6 4 1 5 5
Bud Neck 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Bud Site 0 0 2 1 4 4 3 17 6 15 9 0 0 2 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 16 10 13 17 10 15 19 12 10 5 29 56 55 24 15 24 0 4 2 3 5 3
Endoplasmic Reticulum 3 0 1 0 0 0 0 0 0 0 0 0 0 0 6 2 7 0 0 0 0 2 0
Endosome 0 1 1 0 2 0 0 0 0 0 0 1 2 0 8 5 0 1 3 1 1 2 22
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 3 1 0 2
Mitochondria 1 36 56 100 58 133 201 218 200 184 213 18 6 59 11 5 11 1 4 1 1 3 10
Nucleus 97 371 245 199 309 186 307 381 211 182 190 195 246 228 225 148 169 106 359 231 171 164 133
Nuclear Periphery 0 0 0 1 4 2 9 7 1 3 2 1 2 1 0 1 0 0 0 0 0 0 0
Nucleolus 3 5 1 3 8 10 8 18 8 6 12 11 9 14 4 4 3 2 3 6 0 3 17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 2 3 3 0 3 4 1 1 1 1 2 0 2 5 1 2 2 5 2 11 45
Vac/Vac Membrane 0 19 3 2 11 4 4 3 4 4 6 4 5 8 19 14 11 6 36 28 20 44 58
Unique Cell Count 103 414 271 240 359 246 392 471 283 258 286 231 310 315 270 179 205 128 430 287 208 249 313
Labelled Cell Count 108 448 322 322 417 352 553 669 447 413 442 260 329 368 299 200 227 128 430 287 208 249 313


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 5.1 4.3 4.2 4.5 3.9 3.9 3.8 3.3 3.2 3.0 5.3 5.3 4.5 6.7 7.2 7.2 5.1 5.1 5.6
Std Deviation (1e-4) 0.7 1.0 0.8 1.0 1.3 0.9 1.3 1.0 0.7 0.6 0.7 1.3 1.3 1.1 1.5 1.7 1.9 1.0 1.5 1.5
Intensity Change (Log2) -0.03 0.09 -0.14 -0.11 -0.18 -0.37 -0.41 -0.5 0.32 0.32 0.09 0.64 0.75 0.75 0.27 0.27 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 0.6 0.2 0.1 0.2 0.3 0.1 -1.4 3.7 5.4 5.3 2.5 2.1 3.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 -4.1 -7.3 0 -5.9 -5.4 -4.8
Nucleus -2.5 -1.7 -4.5 -4.1 -3.4 -4.9 -5.8 -6.8 -2.0 -3.7 -5.5 -2.4 -2.4 -2.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 3.2 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.6 0 0 0 0 0 0 0 0 1.3 3.5 3.7 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0547 2.9082 2.8522 2.9393 2.1249 2.645 5.1252 3.6242 3.7745 3.6283 3.3681 3.4137 3.9913 4.1686 4.2223 4.5004 3.8035 4.0604
Actin 0.1428 0 0.0208 0.0409 0.0053 0.0004 0.0601 0 0.0096 0.0259 0.0155 0.002 0.0272 0 0.0005 0.0001 0 0.0003
Bud 0.0015 0 0.0002 0.0004 0.0007 0.0012 0.0013 0.0001 0.0005 0.0013 0.0129 0.0006 0.0001 0 0.0001 0 0 0.0002
Bud Neck 0.0161 0.0002 0.0005 0.0005 0.001 0.0015 0.0156 0.0003 0.0051 0.0155 0.0167 0.0022 0.0006 0.0003 0.0002 0.0001 0.0007 0.001
Bud Periphery 0.0032 0 0.0003 0.0003 0.0013 0.0005 0.0033 0.0001 0.0007 0.0027 0.0388 0.0009 0.0004 0 0.0002 0 0 0.0007
Bud Site 0.0091 0.0002 0.0013 0.0021 0.0024 0.0004 0.0228 0.0002 0.008 0.0102 0.0272 0.0006 0.0004 0.0004 0.0004 0 0.0001 0.0002
Cell Periphery 0.0019 0 0.0001 0 0.0004 0.0001 0.0008 0 0.0004 0.0007 0.0011 0.0001 0.0002 0 0.0001 0 0 0
Cytoplasm 0.0264 0.0011 0.0026 0.004 0.0026 0.0027 0.0329 0.001 0.0018 0.0032 0.0015 0.0035 0.0215 0.0048 0.0004 0.0027 0.0006 0.0093
Cytoplasmic Foci 0.0282 0 0.0023 0.0206 0.0124 0.0077 0.0122 0.0001 0.0068 0.0051 0.0017 0.0009 0.0039 0.0001 0 0.0001 0 0.0004
Eisosomes 0.0031 0 0.0005 0.0002 0.0001 0 0.0007 0 0.0001 0.0003 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0155 0.0001 0.0045 0.0002 0.0007 0.0005 0.0099 0.0001 0.0014 0.0014 0.0014 0.0038 0.0032 0.0003 0.0003 0.0001 0.0001 0.0003
Endosome 0.0494 0.0001 0.0117 0.0035 0.0307 0.0034 0.0222 0.0001 0.0109 0.0384 0.0057 0.0084 0.0111 0.0007 0.0002 0.0001 0.0001 0.0005
Golgi 0.0237 0 0.0092 0.0059 0.0214 0.0007 0.0105 0 0.0064 0.0068 0.0032 0.002 0.0024 0 0 0 0 0
Lipid Particles 0.0247 0 0.0019 0.01 0.016 0.0023 0.0049 0 0.0063 0.0058 0.0011 0.0004 0.0121 0 0 0 0 0.0004
Mitochondria 0.0121 0.0001 0.0035 0.0015 0.0348 0.0011 0.0138 0.0002 0.0047 0.0237 0.0048 0.0055 0.0067 0.0001 0.0015 0.0001 0.0002 0.0021
None 0.0342 0.0001 0.0011 0.0065 0.0039 0.0031 0.0172 0.0001 0.0004 0.0005 0.0003 0.0009 0.008 0.0094 0.0002 0.0281 0.0001 0.0055
Nuclear Periphery 0.0394 0.0028 0.0081 0.0009 0.0047 0.0058 0.0304 0.0039 0.013 0.0043 0.0033 0.0132 0.0265 0.0055 0.005 0.0023 0.0022 0.0019
Nucleolus 0.0134 0.0102 0.0074 0.0066 0.019 0.0206 0.0129 0.0103 0.0115 0.0043 0.0106 0.0184 0.0142 0.0109 0.0087 0.0054 0.0186 0.0167
Nucleus 0.4977 0.9815 0.9051 0.8582 0.7722 0.9392 0.6896 0.9638 0.8879 0.7925 0.84 0.9296 0.8283 0.9491 0.9805 0.9264 0.9377 0.9565
Peroxisomes 0.0225 0 0.0006 0.0195 0.0095 0.0006 0.0042 0 0.0015 0.0178 0.0007 0.0004 0.0021 0 0 0 0 0.0002
Punctate Nuclear 0.0221 0.0029 0.0161 0.0181 0.05 0.0072 0.0195 0.018 0.0195 0.0028 0.0013 0.0036 0.0142 0.0137 0.0007 0.0341 0.0395 0.0032
Vacuole 0.0091 0.0004 0.0014 0.0002 0.0077 0.0009 0.0076 0.0014 0.0026 0.0296 0.0094 0.0016 0.0113 0.0038 0.0005 0.0001 0.0001 0.0006
Vacuole Periphery 0.0039 0.0001 0.0007 0.0002 0.0031 0.0003 0.0076 0.0003 0.0008 0.0072 0.0025 0.0011 0.0054 0.0005 0.0004 0.0001 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.99 8.3921 11.7874 16.5219 27.3864 30.4935 10.9097 7.9769 12.9721 27.8428
Translational Efficiency 1.981 1.451 0.7761 1.1595 1.2746 1.537 0.9082 0.9811 1.02 1.0847

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1525 1435 1595 148 1436 1723 167 1275 2961 3158 1762 1423

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 590.86 791.96 979.03 957.30 668.14 767.66 992.86 862.08 628.34 778.70 980.34 871.98
Standard Deviation 73.61 113.24 130.49 117.09 129.62 92.35 101.33 125.60 111.49 103.08 128.08 128.08
Intensity Change Log 2 0.422611 0.728537 0.696155 0.200317 0.571440 0.367671 0.308916 0.647301 0.531169

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000066 0.000295 0.000380 0.001936 0.000078 0.000179 0.000875 0.001364 0.000072 0.000232 0.000427 0.001424
Bud Neck 0.003834 0.005525 0.009203 0.008176 0.002280 0.005073 0.003823 0.024963 0.003080 0.005278 0.008693 0.023217
Bud Site 0.001464 0.001301 0.002094 0.005542 0.000482 0.001888 0.001147 0.020345 0.000988 0.001621 0.002005 0.018805
Cell Periphery 0.000282 0.000149 0.000083 0.000331 0.000632 0.000115 0.000073 0.000396 0.000452 0.000131 0.000082 0.000389
Cytoplasm 0.146581* 0.031179 0.016682 0.049209 0.054570 0.040236 0.004078 0.054411 0.101958* 0.036120 0.015488 0.053870
Cytoplasmic Foci 0.017233 0.005664 0.000092 0.011307 0.007793 0.005023 0.000116 0.006581 0.012655 0.005314 0.000095 0.007072
Eisosomes 0.000036 0.000022 0.000013 0.000082 0.000882 0.000021 0.000042 0.000043 0.000446 0.000021 0.000015 0.000047
Endoplasmic Reticulum 0.000701 0.004037 0.004406 0.007020 0.002000 0.003133 0.009193 0.006888 0.001331 0.003544 0.004860 0.006902
Endosome 0.000809 0.000689 0.000207 0.016820 0.000642 0.001344 0.000377 0.009546 0.000728 0.001047 0.000223 0.010302
Golgi 0.000182 0.000211 0.000039 0.002065 0.000312 0.000667 0.000060 0.003043 0.000245 0.000459 0.000041 0.002941
Lipid Particles 0.002006 0.001323 0.000100 0.009665 0.003026 0.002256 0.000329 0.001827 0.002501 0.001832 0.000121 0.002642
Mitochondria 0.000879 0.002222 0.000854 0.011355 0.001104 0.004978 0.000727 0.008952 0.000988 0.003726 0.000842 0.009201
Mitotic Spindle 0.000252 0.002859 0.000758 0.033895 0.001504 0.005890 0.000266 0.064585 0.000859 0.004513 0.000711 0.061393
None 0.048433 0.009141 0.002338 0.014042 0.038362 0.005488 0.004671 0.013026 0.043549 0.007148 0.002559 0.013131
Nuclear Periphery 0.000558 0.001306 0.000661 0.001795 0.000989 0.002464 0.000594 0.002158 0.000767 0.001938 0.000654 0.002120
Nuclear Periphery Foci 0.000573 0.000428 0.000186 0.002043 0.000838 0.000552 0.000414 0.002983 0.000701 0.000496 0.000207 0.002886
Nucleolus 0.014495 0.009259 0.000756 0.009986 0.009250 0.008067 0.005062 0.008523 0.011951 0.008609 0.001164 0.008675
Nucleus 0.758018* 0.921525* 0.957668* 0.791323* 0.873320* 0.906435* 0.963954* 0.736641* 0.813936* 0.913292* 0.958264* 0.742328*
Peroxisomes 0.000794 0.001008 0.000054 0.009969 0.000687 0.001422 0.000249 0.001780 0.000742 0.001234 0.000072 0.002631
Vacuole 0.002666 0.001611 0.002785 0.011197 0.001151 0.004235 0.003847 0.024387 0.001931 0.003043 0.002886 0.023015
Vacuole Periphery 0.000138 0.000246 0.000643 0.002241 0.000098 0.000535 0.000102 0.007562 0.000118 0.000404 0.000592 0.007009

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.48 -11.19 -7.59 -6.80 -6.38 -5.34 -9.15 -9.54 -8.73 -1.62 -6.39 -13.81 -11.06 -9.74 -7.68
Bud Neck -2.52 -6.18 -5.96 -5.40 -3.94 -4.14 -2.40 -15.55 -13.49 -7.71 -4.59 -7.54 -16.11 -14.52 -11.03
Bud Site 0.41 -0.97 -4.80 -4.87 -4.55 -3.25 -3.27 -9.30 -8.28 -8.27 -1.95 -2.15 -9.72 -9.26 -8.78
Cell Periphery 3.57 5.61 1.17 -1.50 -3.08 5.18 5.45 3.69 -2.94 -3.57 6.15 7.08 3.43 -2.80 -4.10
Cytoplasm 19.63 23.47 12.68 0.38 -1.70 3.03 12.92 3.93 1.19 -10.51 16.94 23.79 15.33 0.37 -7.04
Cytoplasmic Foci 5.98 11.35 3.62 -0.03 -2.30 2.34 6.79 3.59 1.87 -5.82 6.43 13.15 8.07 2.14 -6.30
Eisosomes 4.79 8.23 -6.13 -7.19 -7.79 3.81 3.65 3.63 -13.01 -1.04 3.87 3.91 3.43 -14.99 -16.71
Endoplasmic Reticulum -11.32 -17.66 -9.91 -6.81 -6.27 -2.97 -10.04 -13.52 -9.05 2.94 -8.83 -16.57 -17.89 -10.88 -7.05
Endosome 0.77 4.84 -2.72 -2.77 -2.96 -2.73 -0.40 -5.86 -4.86 -5.47 -2.03 4.88 -6.41 -5.95 -7.13
Golgi 0.06 4.37 -3.64 -3.60 -4.18 -1.66 2.63 -3.62 -2.33 -4.37 -1.63 4.95 -4.23 -3.41 -5.00
Lipid Particles 1.50 5.85 -1.21 -1.66 -2.55 0.79 2.95 1.88 1.95 -2.37 1.26 5.16 1.72 0.77 -4.04
Mitochondria -2.74 -0.17 -1.68 -0.96 -1.67 -3.87 0.78 -4.21 -0.10 -4.70 -4.69 0.62 -4.77 -1.27 -4.97
Mitotic Spindle -2.73 -1.76 -5.29 -5.14 -5.23 -2.79 1.07 -14.86 -14.09 -15.08 -3.90 -0.70 -15.90 -15.24 -15.79
None 11.43 14.38 9.12 -0.71 -3.49 8.39 8.45 7.33 -2.74 -2.97 14.08 16.44 12.75 -1.73 -6.72
Nuclear Periphery -7.18 -3.37 -7.44 -5.01 -6.85 -3.57 1.08 -7.61 -0.97 -8.45 -5.09 -0.05 -9.94 -3.21 -10.13
Nuclear Periphery Foci 1.79 1.47 -3.37 -3.50 -3.50 0.63 -0.93 -4.75 -4.88 -2.21 1.68 1.08 -5.67 -6.02 -5.89
Nucleolus 4.09 8.93 -3.07 -3.99 -4.86 0.15 -0.33 -9.00 -8.96 -5.28 3.45 10.01 -8.31 -9.99 -12.54
Nucleus -19.31 -25.81 2.64 8.27 9.57 -3.72 -6.73 15.20 19.18 19.52 -16.21 -25.28 11.57 22.18 27.23
Peroxisomes -0.95 5.56 -2.28 -2.08 -2.80 -1.95 1.72 -2.98 -0.70 -4.48 -2.12 6.30 -3.80 -1.96 -6.61
Vacuole 3.19 -0.95 -3.94 -4.09 -3.89 -4.57 -4.15 -10.48 -8.70 -7.22 -2.85 -4.26 -10.90 -10.23 -10.17
Vacuole Periphery -2.20 -0.90 -1.93 -1.63 -0.54 -3.61 -1.92 -3.48 -2.93 -3.43 -4.09 -0.94 -3.56 -3.16 -2.65