Standard name | |
---|---|
Description | Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 12 | 8 | 7 | 14 | 9 | 10 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud | 2 | 1 | 1 | 0 | 0 | 1 | 5 | 2 | 7 | 11 | 5 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 7 | 7 | 23 | 19 | 21 | 18 | 18 | 13 | 13 | 8 | 15 | 19 | 0 | 0 | 1 | 9 | 13 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 335 | 364 | 519 | 515 | 406 | 482 | 532 | 378 | 419 | 361 | 260 | 350 | 251 | 268 | 302 | 189 | 156 | 110 | 324 | 364 | 531 | 129 | 223 | 197 |
Endoplasmic Reticulum | 0 | 5 | 7 | 5 | 30 | 44 | 83 | 31 | 29 | 46 | 27 | 30 | 0 | 3 | 3 | 13 | 4 | 1 | 1 | 0 | 2 | 7 | 5 | 4 |
Endosome | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 4 | 0 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4 | 0 | 1 | 1 | 1 | 5 | 6 | 5 | 39 | 21 | 55 | 33 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 1 | 2 |
Nucleus | 5 | 3 | 3 | 3 | 1 | 3 | 5 | 15 | 4 | 11 | 8 | 13 | 1 | 3 | 0 | 1 | 1 | 1 | 0 | 6 | 2 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 0 | 5 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 3 | 2 | 6 | 4 | 10 | 18 | 26 | 141 | 77 | 90 | 131 | 232 | 0 | 0 | 0 | 1 | 2 | 5 | 3 | 7 | 9 | 2 | 3 | 4 |
Unique Cell Count | 345 | 374 | 533 | 525 | 451 | 561 | 656 | 571 | 566 | 530 | 473 | 651 | 252 | 272 | 305 | 195 | 162 | 115 | 344 | 385 | 559 | 144 | 243 | 219 |
Labelled Cell Count | 358 | 383 | 560 | 547 | 471 | 585 | 683 | 602 | 602 | 562 | 511 | 704 | 253 | 275 | 306 | 213 | 176 | 123 | 344 | 385 | 559 | 144 | 243 | 219 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.5 | 13.1 | 12.2 | 11.5 | 13.8 | 12.1 | 12.0 | 11.7 | 10.3 | 9.7 | 9.1 | 10.3 | 16.4 | 17.7 | 17.1 | 29.3 | 30.6 | 29.6 | 12.9 | 14.0 | 15.7 |
Std Deviation (1e-4) | 2.7 | 2.6 | 1.9 | 1.5 | 2.0 | 1.9 | 1.9 | 2.3 | 1.8 | 1.7 | 1.6 | 2.0 | 3.1 | 3.5 | 3.1 | 7.9 | 8.4 | 8.8 | 2.1 | 2.3 | 3.0 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.17 | -0.01 | -0.02 | -0.06 | -0.25 | -0.33 | -0.42 | -0.24 | 0.42 | 0.53 | 0.48 | 1.26 | 1.32 | 1.28 | 0.08 | 0.19 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 3.4 | 0 | 0 | 3.7 | 0 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.6 | 0.3 | -1.0 | -1.5 | -1.9 | -1.9 | -2.7 | -0.9 | -1.3 | -3.3 | -3.5 | -3.3 | 0.2 | 1.9 | 0.4 |
Cytoplasm | 0.8 | -4.8 | -6.8 | -8.7 | -13.3 | -10.9 | -12.6 | -16.1 | -16.9 | 2.1 | 1.0 | 1.6 | -0.3 | -0.7 | -1.0 |
Endoplasmic Reticulum | -0.6 | 4.4 | 5.1 | 7.4 | 3.7 | 3.5 | 5.5 | 3.9 | 3.2 | 0 | 0 | 0 | 3.9 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 5.9 | 4.3 | 7.9 | 5.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 2.7 | 0 | 2.2 | 1.7 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.3 | 2.4 | 3.0 | 11.5 | 7.8 | 9.0 | 12.3 | 14.7 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.8103 | 11.7449 | 10.5655 | 9.9365 | 8.7588 | 10.9399 | 11.5339 | 15.3121 | 13.5841 | 13.3926 | 11.7885 | 13.5329 | 10.9742 | 14.4862 | 12.9749 | 12.5494 | 12.4672 | 12.789 |
Actin | 0.0236 | 0.0012 | 0.0088 | 0.0024 | 0.0241 | 0.0012 | 0.01 | 0.0015 | 0.0063 | 0.0007 | 0.0044 | 0.0023 | 0.0143 | 0.0006 | 0.0066 | 0.0042 | 0.0014 | 0.0025 |
Bud | 0.0017 | 0.0003 | 0.0003 | 0.004 | 0.0006 | 0.0003 | 0.0007 | 0.0029 | 0.0005 | 0.0013 | 0.001 | 0.0008 | 0.0006 | 0.0002 | 0.0008 | 0.0011 | 0.0014 | 0.0008 |
Bud Neck | 0.0058 | 0.0003 | 0.0005 | 0.0006 | 0.0019 | 0.0021 | 0.0013 | 0.0004 | 0.0009 | 0.0048 | 0.0026 | 0.0018 | 0.0013 | 0.0003 | 0.001 | 0.0011 | 0.0013 | 0.0077 |
Bud Periphery | 0.002 | 0.0001 | 0.0001 | 0.0072 | 0.001 | 0.0002 | 0.0004 | 0.0006 | 0.0003 | 0.0004 | 0.0005 | 0.0011 | 0.0004 | 0 | 0.0004 | 0.0011 | 0.0014 | 0.0004 |
Bud Site | 0.0033 | 0.0003 | 0.0004 | 0.0001 | 0.0008 | 0.0001 | 0.0011 | 0.0045 | 0.0007 | 0.0287 | 0.0007 | 0.0002 | 0.001 | 0.0003 | 0.0009 | 0.0009 | 0.0003 | 0.0005 |
Cell Periphery | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.7811 | 0.9663 | 0.9503 | 0.9702 | 0.8595 | 0.9376 | 0.8734 | 0.9676 | 0.9355 | 0.9344 | 0.8305 | 0.9429 | 0.8864 | 0.9811 | 0.9415 | 0.941 | 0.9587 | 0.9417 |
Cytoplasmic Foci | 0.0238 | 0.0059 | 0.0046 | 0.0021 | 0.0075 | 0.0071 | 0.0218 | 0.0082 | 0.0119 | 0.0124 | 0.0248 | 0.0109 | 0.0146 | 0.0044 | 0.0143 | 0.0046 | 0.0107 | 0.014 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0163 | 0.0047 | 0.0025 | 0.0018 | 0.0028 | 0.0068 | 0.005 | 0.0025 | 0.002 | 0.0011 | 0.0039 | 0.0059 | 0.0078 | 0.0011 | 0.0028 | 0.0057 | 0.0009 | 0.0074 |
Endosome | 0.0415 | 0.0064 | 0.0128 | 0.0021 | 0.0339 | 0.015 | 0.026 | 0.0059 | 0.0105 | 0.0064 | 0.0364 | 0.011 | 0.0247 | 0.0027 | 0.0092 | 0.0043 | 0.007 | 0.0088 |
Golgi | 0.0082 | 0.0011 | 0.0016 | 0.0003 | 0.006 | 0.004 | 0.0096 | 0.0012 | 0.0025 | 0.0017 | 0.013 | 0.0028 | 0.0054 | 0.0003 | 0.005 | 0.0015 | 0.0051 | 0.0011 |
Lipid Particles | 0.0148 | 0.0001 | 0.0017 | 0 | 0.0059 | 0.0015 | 0.0108 | 0 | 0.002 | 0.0003 | 0.0257 | 0.0013 | 0.0049 | 0 | 0.0036 | 0.0003 | 0.005 | 0.0001 |
Mitochondria | 0.0095 | 0.0003 | 0.003 | 0.0009 | 0.0061 | 0.0055 | 0.0108 | 0.0004 | 0.0186 | 0.0016 | 0.0157 | 0.006 | 0.0064 | 0.0003 | 0.0027 | 0.0255 | 0.0013 | 0.0019 |
None | 0.0033 | 0.0005 | 0.001 | 0.0006 | 0.0011 | 0.0007 | 0.0044 | 0.0005 | 0.0006 | 0.0003 | 0.0026 | 0.0008 | 0.0036 | 0.0004 | 0.0009 | 0.0011 | 0.0003 | 0.0008 |
Nuclear Periphery | 0.0169 | 0.0034 | 0.002 | 0.0014 | 0.0063 | 0.0045 | 0.0044 | 0.0006 | 0.0013 | 0.0003 | 0.0115 | 0.003 | 0.0072 | 0.0008 | 0.0013 | 0.0012 | 0.0004 | 0.0015 |
Nucleolus | 0.0059 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0005 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0.0002 |
Nucleus | 0.0132 | 0.0052 | 0.0046 | 0.0037 | 0.0067 | 0.0081 | 0.0045 | 0.0014 | 0.0026 | 0.0017 | 0.0075 | 0.0029 | 0.0116 | 0.0055 | 0.003 | 0.0038 | 0.0022 | 0.0045 |
Peroxisomes | 0.0066 | 0.0002 | 0.0009 | 0.0001 | 0.021 | 0.0005 | 0.0045 | 0.0003 | 0.0008 | 0.0025 | 0.0035 | 0.0034 | 0.0024 | 0.0001 | 0.0027 | 0.0007 | 0.0019 | 0.0005 |
Punctate Nuclear | 0.0099 | 0.0002 | 0.0003 | 0.0001 | 0.001 | 0.0019 | 0.0059 | 0.0001 | 0.0014 | 0.0001 | 0.0096 | 0.0009 | 0.0012 | 0.0001 | 0.0017 | 0.0002 | 0.0001 | 0.0034 |
Vacuole | 0.0087 | 0.0028 | 0.0031 | 0.0018 | 0.0083 | 0.0018 | 0.0036 | 0.0008 | 0.001 | 0.0007 | 0.0038 | 0.0011 | 0.004 | 0.0013 | 0.001 | 0.001 | 0.0004 | 0.0015 |
Vacuole Periphery | 0.0029 | 0.0005 | 0.0013 | 0.0007 | 0.005 | 0.0009 | 0.0013 | 0.0002 | 0.0003 | 0.0003 | 0.0017 | 0.0006 | 0.0014 | 0.0002 | 0.0003 | 0.0005 | 0.0002 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 95.9482 | 74.2434 | 87.9415 | 98.6214 | 72.2329 | 78.3659 | 99.1962 | 101.4882 | 110.223 | 87.6235 |
Translational Efficiency | 0.9865 | 1.0953 | 1.0434 | 0.9578 | 0.9747 | 1.1843 | 0.9159 | 0.8303 | 0.881 | 0.8648 |
Dataset | Hit |
---|---|
Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |