Standard name
Description Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.05 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.97 0.98 0.9 0.86 0.81 0.66 0.74 0.68 0.55 0.54 1.0 0.99 0.99 0.97 0.96 0.96 0.94 0.95 0.95 0.9 0.92 0.9
Endoplasmic Reticulum 0 0 0 0 0.07 0.08 0.13 0.05 0.05 0.09 0.06 0.05 0 0 0 0.07 0 0 0 0 0 0.06 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.07 0 0.12 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.25 0.14 0.17 0.28 0.36 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 12 8 7 14 9 10 12 0 0 0 0 0 0 1 0 0 0 0 0
Bud 2 1 1 0 0 1 5 2 7 11 5 4 0 1 0 0 0 0 1 1 1 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 7 7 23 19 21 18 18 13 13 8 15 19 0 0 1 9 13 6 0 0 0 0 0 0
Cytoplasm 335 364 519 515 406 482 532 378 419 361 260 350 251 268 302 189 156 110 324 364 531 129 223 197
Endoplasmic Reticulum 0 5 7 5 30 44 83 31 29 46 27 30 0 3 3 13 4 1 1 0 2 7 5 4
Endosome 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 2 0 4 0 1 0
Golgi 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0
Mitochondria 4 0 1 1 1 5 6 5 39 21 55 33 1 0 0 0 0 0 1 0 3 0 1 2
Nucleus 5 3 3 3 1 3 5 15 4 11 8 13 1 3 0 1 1 1 0 6 2 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 7 0 5 0 5 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2 0 0 0 0 1 0 2 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 3 2 6 4 10 18 26 141 77 90 131 232 0 0 0 1 2 5 3 7 9 2 3 4
Unique Cell Count 345 374 533 525 451 561 656 571 566 530 473 651 252 272 305 195 162 115 344 385 559 144 243 219
Labelled Cell Count 358 383 560 547 471 585 683 602 602 562 511 704 253 275 306 213 176 123 344 385 559 144 243 219


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.5 13.1 12.2 11.5 13.8 12.1 12.0 11.7 10.3 9.7 9.1 10.3 16.4 17.7 17.1 29.3 30.6 29.6 12.9 14.0 15.7
Std Deviation (1e-4) 2.7 2.6 1.9 1.5 2.0 1.9 1.9 2.3 1.8 1.7 1.6 2.0 3.1 3.5 3.1 7.9 8.4 8.8 2.1 2.3 3.0
Intensity Change (Log2) – – – -0.09 0.17 -0.01 -0.02 -0.06 -0.25 -0.33 -0.42 -0.24 0.42 0.53 0.48 1.26 1.32 1.28 0.08 0.19 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 3.4 0 0 3.7 0 0 3.2 0 0 0 0 0 0
Bud 0 0 0 0 0 0 2.9 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.6 0.3 -1.0 -1.5 -1.9 -1.9 -2.7 -0.9 -1.3 -3.3 -3.5 -3.3 0.2 1.9 0.4
Cytoplasm 0.8 -4.8 -6.8 -8.7 -13.3 -10.9 -12.6 -16.1 -16.9 2.1 1.0 1.6 -0.3 -0.7 -1.0
Endoplasmic Reticulum -0.6 4.4 5.1 7.4 3.7 3.5 5.5 3.9 3.2 0 0 0 3.9 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 5.9 4.3 7.9 5.0 0 0 0 0 0 0
Nucleus 0 0 0 0 2.7 0 2.2 1.7 2.1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.3 2.4 3.0 11.5 7.8 9.0 12.3 14.7 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.8103 11.7449 10.5655 9.9365 8.7588 10.9399 11.5339 15.3121 13.5841 13.3926 11.7885 13.5329 10.9742 14.4862 12.9749 12.5494 12.4672 12.789
Actin 0.0236 0.0012 0.0088 0.0024 0.0241 0.0012 0.01 0.0015 0.0063 0.0007 0.0044 0.0023 0.0143 0.0006 0.0066 0.0042 0.0014 0.0025
Bud 0.0017 0.0003 0.0003 0.004 0.0006 0.0003 0.0007 0.0029 0.0005 0.0013 0.001 0.0008 0.0006 0.0002 0.0008 0.0011 0.0014 0.0008
Bud Neck 0.0058 0.0003 0.0005 0.0006 0.0019 0.0021 0.0013 0.0004 0.0009 0.0048 0.0026 0.0018 0.0013 0.0003 0.001 0.0011 0.0013 0.0077
Bud Periphery 0.002 0.0001 0.0001 0.0072 0.001 0.0002 0.0004 0.0006 0.0003 0.0004 0.0005 0.0011 0.0004 0 0.0004 0.0011 0.0014 0.0004
Bud Site 0.0033 0.0003 0.0004 0.0001 0.0008 0.0001 0.0011 0.0045 0.0007 0.0287 0.0007 0.0002 0.001 0.0003 0.0009 0.0009 0.0003 0.0005
Cell Periphery 0.0007 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0004 0.0002 0.0002 0.0001 0.0001 0.0002 0 0.0001 0.0001 0.0002 0.0001
Cytoplasm 0.7811 0.9663 0.9503 0.9702 0.8595 0.9376 0.8734 0.9676 0.9355 0.9344 0.8305 0.9429 0.8864 0.9811 0.9415 0.941 0.9587 0.9417
Cytoplasmic Foci 0.0238 0.0059 0.0046 0.0021 0.0075 0.0071 0.0218 0.0082 0.0119 0.0124 0.0248 0.0109 0.0146 0.0044 0.0143 0.0046 0.0107 0.014
Eisosomes 0.0002 0 0 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0163 0.0047 0.0025 0.0018 0.0028 0.0068 0.005 0.0025 0.002 0.0011 0.0039 0.0059 0.0078 0.0011 0.0028 0.0057 0.0009 0.0074
Endosome 0.0415 0.0064 0.0128 0.0021 0.0339 0.015 0.026 0.0059 0.0105 0.0064 0.0364 0.011 0.0247 0.0027 0.0092 0.0043 0.007 0.0088
Golgi 0.0082 0.0011 0.0016 0.0003 0.006 0.004 0.0096 0.0012 0.0025 0.0017 0.013 0.0028 0.0054 0.0003 0.005 0.0015 0.0051 0.0011
Lipid Particles 0.0148 0.0001 0.0017 0 0.0059 0.0015 0.0108 0 0.002 0.0003 0.0257 0.0013 0.0049 0 0.0036 0.0003 0.005 0.0001
Mitochondria 0.0095 0.0003 0.003 0.0009 0.0061 0.0055 0.0108 0.0004 0.0186 0.0016 0.0157 0.006 0.0064 0.0003 0.0027 0.0255 0.0013 0.0019
None 0.0033 0.0005 0.001 0.0006 0.0011 0.0007 0.0044 0.0005 0.0006 0.0003 0.0026 0.0008 0.0036 0.0004 0.0009 0.0011 0.0003 0.0008
Nuclear Periphery 0.0169 0.0034 0.002 0.0014 0.0063 0.0045 0.0044 0.0006 0.0013 0.0003 0.0115 0.003 0.0072 0.0008 0.0013 0.0012 0.0004 0.0015
Nucleolus 0.0059 0 0.0001 0 0.0003 0.0001 0.0002 0 0.0001 0.0002 0.0005 0 0.0003 0 0 0.0001 0 0.0002
Nucleus 0.0132 0.0052 0.0046 0.0037 0.0067 0.0081 0.0045 0.0014 0.0026 0.0017 0.0075 0.0029 0.0116 0.0055 0.003 0.0038 0.0022 0.0045
Peroxisomes 0.0066 0.0002 0.0009 0.0001 0.021 0.0005 0.0045 0.0003 0.0008 0.0025 0.0035 0.0034 0.0024 0.0001 0.0027 0.0007 0.0019 0.0005
Punctate Nuclear 0.0099 0.0002 0.0003 0.0001 0.001 0.0019 0.0059 0.0001 0.0014 0.0001 0.0096 0.0009 0.0012 0.0001 0.0017 0.0002 0.0001 0.0034
Vacuole 0.0087 0.0028 0.0031 0.0018 0.0083 0.0018 0.0036 0.0008 0.001 0.0007 0.0038 0.0011 0.004 0.0013 0.001 0.001 0.0004 0.0015
Vacuole Periphery 0.0029 0.0005 0.0013 0.0007 0.005 0.0009 0.0013 0.0002 0.0003 0.0003 0.0017 0.0006 0.0014 0.0002 0.0003 0.0005 0.0002 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 95.9482 74.2434 87.9415 98.6214 72.2329 78.3659 99.1962 101.4882 110.223 87.6235
Translational Efficiency 0.9865 1.0953 1.0434 0.9578 0.9747 1.1843 0.9159 0.8303 0.881 0.8648

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘