Standard name
Human Ortholog
Description Member of the NineTeen Complex (NTC); NTC contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs; interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.08 0.08 0.1 0.09 – 0.07 0.1 0.05 0.06 0.07 0.16 0.17 0.16 0.08 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.05 0 0 0.05 0.42 0.1 – 0.43 0.32 0.42 0.32 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.89 0.94 0.96 0.92 0.89 0.78 0.84 – 0.79 0.76 0.8 0.8 0.92 0.82 0.77 0.77 0.82 0.89 0.82 0.93 0.89 0.78 0.77 0.62
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.07
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0.06 0.06 0.06 0 0.06 0.09 0.1 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 5 0 – 2 8 5 5 0 0 0 1 0 1 1 0 1 1 3 5
Bud Neck 0 0 0 0 0 0 1 – 1 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 0 0 0 11 1 – 6 11 17 26 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 – 0 0 1 0 0 1 0 0 1 0 0 0 0 0 0 0
Cytoplasm 8 5 11 13 14 26 32 – 21 31 20 30 23 56 66 22 16 12 1 0 2 0 1 2
Endoplasmic Reticulum 0 0 1 0 0 1 0 – 0 0 0 0 0 1 1 3 2 1 0 0 0 0 1 2
Endosome 0 0 0 0 0 0 2 – 0 1 0 3 0 2 4 2 5 0 5 0 0 3 3 3
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 1 1 0 1 1 0 0 0 0 0 1
Mitochondria 19 12 2 5 8 106 36 – 125 99 166 169 2 0 0 3 6 6 2 0 3 0 4 6
Nucleus 170 237 272 142 149 199 306 – 231 240 316 418 284 284 293 107 162 184 169 232 260 152 232 149
Nuclear Periphery 1 0 0 3 1 1 0 – 4 2 3 6 0 2 1 1 5 1 0 0 1 1 0 1
Nucleolus 14 2 0 0 0 0 1 – 8 8 10 8 0 0 2 1 0 3 4 2 2 9 16 17
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 1 5 0 1 0 0 0 0 0 0 0
SpindlePole 0 1 9 0 1 2 6 – 3 5 5 7 0 2 2 0 3 0 4 0 1 3 2 3
Vac/Vac Membrane 2 8 0 1 2 1 10 – 4 2 2 7 2 8 16 11 11 12 13 10 16 17 29 42
Unique Cell Count 190 251 284 155 168 254 364 294 314 395 525 309 348 382 139 197 206 207 249 291 194 300 240
Labelled Cell Count 215 265 295 164 175 352 396 405 409 546 680 311 358 391 151 213 221 207 249 291 194 300 240


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 6.0 6.0 6.0 6.0 4.0 5.2 – 4.5 4.8 4.5 4.6 6.1 6.5 6.3 8.3 7.7 8.2 5.7 5.9 6.2
Std Deviation (1e-4) 0.9 0.9 1.3 1.6 1.7 1.2 1.7 – 1.6 1.7 1.4 1.2 1.2 1.8 1.8 2.2 1.8 2.5 1.6 1.5 1.9
Intensity Change (Log2) – – – -0.01 0.0 -0.6 -0.22 – -0.42 -0.32 -0.42 -0.4 0.02 0.12 0.07 0.46 0.36 0.44 -0.08 -0.05 0.03

WT3RAP60RAP140RAP220RAP300RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.0 2.0 2.9 2.5 – 1.7 2.9 0.7 1.1 1.9 5.0 5.4 4.3 2.0 1.0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.9 – 0 0 0 0 0 0 0 0 0 0
Nucleus -1.8 -2.9 -6.1 -4.8 – -6.2 -6.7 -6.0 -6.2 -1.9 -5.4 -6.8 -5.9 -4.9 -2.8
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 -1.3 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 3.5 0 0 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.223 0.1663 -0.0054 -0.2183 -0.408 0.0397 2.0644 1.7956 1.5974 1.3416 1.2517 1.7331 4.5449 5.0127 4.6015 4.4133 4.1341 4.4944
Actin 0.0135 0 0.0012 0 0.0001 0.0007 0.0054 0 0.0019 0.0304 0.0147 0.0065 0 0 0 0 0.0012 0
Bud 0.0003 0 0.0001 0 0.0001 0 0.0005 0 0.0005 0.0016 0.0001 0.0002 0 0 0 0 0.0003 0
Bud Neck 0.0021 0 0.0007 0 0.0002 0.0008 0.0023 0 0.0012 0.0031 0.0011 0.0014 0.0002 0.0001 0 0 0.001 0.0005
Bud Periphery 0.0012 0 0.0002 0 0.0001 0.0001 0.001 0 0.0007 0.0038 0.0003 0.0008 0 0 0 0 0.0006 0
Bud Site 0.0004 0 0.0001 0 0.0002 0.0001 0.0043 0 0.0035 0.002 0.0003 0.0003 0.0001 0 0 0 0.0006 0
Cell Periphery 0.0002 0 0.0001 0 0 0 0.0008 0 0.0003 0.0001 0 0.0001 0 0 0 0 0.0001 0
Cytoplasm 0.0018 0.0002 0.001 0.0001 0.0037 0.0006 0.0073 0.0031 0.0019 0.0003 0.0002 0.0004 0.0004 0.001 0.0004 0.0003 0.0024 0.0002
Cytoplasmic Foci 0.0026 0 0.0005 0 0.0034 0.0019 0.0168 0.0002 0.0019 0.0051 0.0041 0.0014 0.0005 0 0 0 0.0034 0
Eisosomes 0.0002 0 0.0001 0 0 0 0.0001 0 0.0001 0.0002 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0014 0 0.0005 0 0.0004 0.0003 0.0026 0.0002 0.001 0.0001 0.0005 0.0011 0.0001 0 0 0 0.0012 0
Endosome 0.0143 0 0.0005 0 0.0265 0.0036 0.0298 0.0002 0.0095 0.002 0.0031 0.0081 0.0006 0 0 0 0.0036 0
Golgi 0.0031 0 0.0003 0 0.0014 0.0007 0.012 0 0.0025 0.003 0.0036 0.0063 0.0001 0 0 0 0.0006 0
Lipid Particles 0.0048 0 0.0056 0 0.0005 0.005 0.0478 0 0.0016 0.0041 0.0195 0.0007 0.0004 0 0 0 0.0007 0
Mitochondria 0.0051 0.0002 0.004 0 0.0014 0.001 0.0152 0.0001 0.0051 0.0155 0.0072 0.0109 0.0001 0.0001 0.0001 0 0.0009 0
None 0.0021 0 0.0004 0 0.0016 0.0003 0.0012 0.004 0.0014 0.0005 0.0001 0.0001 0.0011 0.0012 0.0053 0.0003 0.0004 0
Nuclear Periphery 0.0179 0.0105 0.008 0.0008 0.0244 0.007 0.0272 0.0047 0.0125 0.0008 0.0253 0.0083 0.0013 0.0028 0.0012 0.0006 0.0026 0.0004
Nucleolus 0.0111 0.0063 0.005 0.0023 0.0195 0.0195 0.0205 0.0049 0.0052 0.0024 0.0305 0.0247 0.0068 0.0062 0.0044 0.0033 0.0134 0.0168
Nucleus 0.9053 0.9819 0.9682 0.9965 0.9105 0.9507 0.7711 0.9808 0.941 0.9133 0.8442 0.9185 0.9833 0.9838 0.9877 0.9941 0.9612 0.9806
Peroxisomes 0.0008 0 0.0011 0 0.0003 0.0011 0.0117 0 0.0005 0.0094 0.0217 0.0023 0.0001 0 0 0 0.0005 0
Punctate Nuclear 0.0077 0.0007 0.0016 0.0001 0.0031 0.0058 0.0121 0.0015 0.0025 0.0018 0.0215 0.0046 0.0047 0.0044 0.0007 0.0012 0.0042 0.0013
Vacuole 0.0024 0 0.0005 0 0.0018 0.0004 0.0079 0.0001 0.0036 0.0002 0.0007 0.0016 0.0002 0.0001 0 0 0.001 0
Vacuole Periphery 0.0017 0.0001 0.0003 0 0.0009 0.0003 0.0026 0 0.0015 0.0002 0.0013 0.0013 0 0 0 0 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.5716 11.1455 12.4057 15.2617 13.1342 23.3643 15.8473 10.4582 16.6254 14.4999
Translational Efficiency 3.0542 1.5844 1.709 1.7249 1.7603 1.0331 1.3567 2.0807 1.4823 1.5917

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2018 1268 245 1723 1636 1830 356 113 3654 3098 601 1836

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 752.83 800.57 1007.04 934.21 722.41 835.23 959.58 1027.19 739.21 821.04 978.93 939.93
Standard Deviation 97.43 111.42 118.87 140.86 89.46 117.21 119.31 130.02 95.15 116.13 121.39 141.98
Intensity Change Log 2 — 0.088703 0.419725 0.311423 — 0.209356 0.409585 0.507813 — 0.149047 0.414769 0.410934

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000122 0.000650 0.001159 0.001679 0.000132 0.000243 0.000777 0.001146 0.000126 0.000409 0.000933 0.001646
Bud Neck 0.002571 0.010553 0.006499 0.021850 0.001890 0.005342 0.008633 0.006536 0.002266 0.007475 0.007763 0.020907
Bud Site 0.000572 0.002277 0.000812 0.006825 0.000570 0.001216 0.000678 0.001256 0.000571 0.001650 0.000732 0.006482
Cell Periphery 0.000102 0.000214 0.000048 0.000140 0.000071 0.000116 0.000043 0.000220 0.000088 0.000156 0.000045 0.000145
Cytoplasm 0.009283 0.010003 0.000854 0.014929 0.008568 0.004583 0.001322 0.006958 0.008963 0.006801 0.001131 0.014439
Cytoplasmic Foci 0.000935 0.001285 0.000047 0.000542 0.001388 0.001842 0.000018 0.000072 0.001138 0.001614 0.000030 0.000513
Eisosomes 0.000011 0.000022 0.000040 0.000018 0.000011 0.000015 0.000025 0.000034 0.000011 0.000018 0.000031 0.000019
Endoplasmic Reticulum 0.002481 0.009301 0.008522 0.006138 0.002496 0.004123 0.005769 0.008824 0.002488 0.006242 0.006891 0.006303
Endosome 0.000248 0.000803 0.000206 0.001131 0.000246 0.001038 0.000132 0.000513 0.000247 0.000941 0.000162 0.001093
Golgi 0.000086 0.000208 0.000067 0.000752 0.000179 0.001004 0.000055 0.000090 0.000128 0.000678 0.000060 0.000711
Lipid Particles 0.000286 0.000637 0.000201 0.000220 0.000435 0.000508 0.000085 0.000197 0.000353 0.000561 0.000132 0.000219
Mitochondria 0.002089 0.002003 0.000887 0.007265 0.000892 0.003937 0.000777 0.001069 0.001553 0.003145 0.000821 0.006884
Mitotic Spindle 0.001128 0.002116 0.000066 0.009971 0.001353 0.002150 0.000197 0.000443 0.001229 0.002136 0.000143 0.009385
None 0.005057 0.007619 0.000978 0.006199 0.006135 0.004466 0.003517 0.006029 0.005540 0.005756 0.002482 0.006188
Nuclear Periphery 0.000921 0.001088 0.000373 0.002247 0.000739 0.001334 0.000334 0.000943 0.000839 0.001233 0.000350 0.002166
Nuclear Periphery Foci 0.000353 0.000592 0.000092 0.000496 0.000255 0.000518 0.000061 0.000261 0.000309 0.000548 0.000074 0.000482
Nucleolus 0.008115 0.013437 0.006435 0.002435 0.005592 0.008131 0.006807 0.013545 0.006986 0.010303 0.006656 0.003119
Nucleus 0.964658 0.934382 0.969910 0.911771 0.967937 0.956635 0.969478 0.948302 0.966126 0.947527 0.969654 0.914019
Peroxisomes 0.000406 0.001089 0.000297 0.000135 0.000540 0.000760 0.000197 0.000296 0.000466 0.000895 0.000238 0.000145
Vacuole 0.000422 0.001324 0.002402 0.004230 0.000467 0.001345 0.001016 0.003102 0.000442 0.001336 0.001581 0.004160
Vacuole Periphery 0.000152 0.000397 0.000107 0.001027 0.000103 0.000697 0.000081 0.000164 0.000130 0.000574 0.000092 0.000974

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.55 -11.49 -2.36 -1.30 0.12 -2.80 -12.88 -6.48 -6.03 -3.34 -7.51 -16.97 -2.58 -1.97 -0.45
Bud Neck -7.60 -4.46 -16.12 -9.19 -8.51 -6.49 -7.85 -6.77 -3.79 0.40 -9.69 -8.71 -16.73 -12.42 -9.41
Bud Site -2.54 -2.54 -5.34 -2.81 -4.72 -1.62 -1.42 -3.51 -2.35 -3.12 -3.05 -2.68 -5.51 -3.96 -4.98
Cell Periphery -3.22 3.07 -0.82 2.90 -5.17 -1.74 2.56 -1.29 -0.79 -1.54 -3.18 4.00 -2.26 1.49 -5.79
Cytoplasm -0.45 8.43 -1.84 -1.22 -9.55 3.01 7.21 -0.31 -0.99 -1.62 2.12 10.99 -2.20 -3.77 -9.48
Cytoplasmic Foci -0.51 3.54 2.41 2.25 -2.72 -0.47 3.15 2.78 3.34 -1.97 -0.88 4.61 3.39 3.75 -2.99
Eisosomes -7.25 -9.84 -11.56 -0.49 5.94 -2.64 -9.00 -6.09 -5.64 -3.54 -7.23 -13.34 -12.83 -5.82 4.88
Endoplasmic Reticulum -12.46 -11.26 -14.56 3.37 2.70 -5.79 -9.20 -8.02 -6.52 -4.36 -13.36 -14.32 -16.43 -3.30 -0.13
Endosome -3.06 0.19 -2.49 -0.08 -2.61 -3.28 1.38 -2.83 0.82 -3.38 -4.32 1.21 -2.69 0.46 -2.98
Golgi -1.59 0.20 -1.42 -0.88 -1.45 -2.02 1.03 0.18 2.13 -2.56 -2.31 1.06 -1.24 0.77 -1.49
Lipid Particles -1.55 1.18 1.43 1.90 0.55 -0.21 3.32 0.61 0.70 -4.18 -1.39 3.84 2.30 2.56 -1.43
Mitochondria 0.03 1.47 -2.53 -3.00 -4.05 -3.07 -0.27 -1.78 2.61 -3.14 -2.31 1.36 -3.29 -1.63 -4.13
Mitotic Spindle -0.92 1.85 -5.18 -4.16 -5.95 -0.92 1.54 -1.02 -0.92 -1.14 -1.36 2.33 -5.30 -4.67 -5.98
None -2.12 6.50 -0.01 2.18 -7.40 1.38 2.63 0.73 -0.20 -1.02 -0.22 4.85 0.67 0.84 -4.27
Nuclear Periphery -1.47 4.64 -6.66 -5.98 -8.14 -1.98 2.76 -3.13 -1.12 -3.99 -2.17 5.41 -7.17 -5.08 -8.49
Nuclear Periphery Foci -1.23 1.68 -1.17 0.14 -3.52 -1.49 2.02 -1.68 -0.93 -2.17 -1.74 2.44 -1.91 -0.26 -4.02
Nucleolus -4.39 1.61 5.45 9.23 3.13 -2.79 -1.95 -1.53 -0.73 -0.77 -4.51 -0.41 4.89 9.25 4.17
Nucleus 7.06 -0.13 11.79 4.54 11.84 2.71 0.97 3.64 2.74 3.40 6.29 0.58 13.09 8.07 12.87
Peroxisomes -1.53 0.48 1.36 2.43 5.11 -0.42 2.03 1.18 1.25 -2.55 -1.42 1.70 2.46 3.09 4.07
Vacuole -4.35 -7.66 -7.80 -6.44 -3.89 -2.72 -3.80 -3.94 -3.60 -3.50 -4.57 -8.05 -8.40 -7.03 -5.79
Vacuole Periphery -3.47 1.17 -2.11 -0.95 -2.27 -3.69 0.34 -2.55 2.90 -3.25 -4.47 1.35 -2.25 -0.06 -2.38
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Member of the NineTeen Complex (NTC); NTC contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs; interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Isy1

Isy1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Isy1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available