Standard name
Description Member of the NineTeen Complex (NTC); NTC contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs; interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.08 0.08 0.1 0.09 0.07 0.1 0.05 0.06 0.07 0.16 0.17 0.16 0.08 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.05 0 0 0.05 0.42 0.1 0.43 0.32 0.42 0.32 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.89 0.94 0.96 0.92 0.89 0.78 0.84 0.79 0.76 0.8 0.8 0.92 0.82 0.77 0.77 0.82 0.89 0.82 0.93 0.89 0.78 0.77 0.62
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.06 0.06 0 0.06 0.09 0.1 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 5 0 2 8 5 5 0 0 0 1 0 1 1 0 1 1 3 5
Bud Neck 0 0 0 0 0 0 1 1 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 0 0 0 11 1 6 11 17 26 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 1 0 0 1 0 0 1 0 0 1 0 0 0 0 0 0 0
Cytoplasm 8 5 11 13 14 26 32 21 31 20 30 23 56 66 22 16 12 1 0 2 0 1 2
Endoplasmic Reticulum 0 0 1 0 0 1 0 0 0 0 0 0 1 1 3 2 1 0 0 0 0 1 2
Endosome 0 0 0 0 0 0 2 0 1 0 3 0 2 4 2 5 0 5 0 0 3 3 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 1 0 0 0 0 0 1
Mitochondria 19 12 2 5 8 106 36 125 99 166 169 2 0 0 3 6 6 2 0 3 0 4 6
Nucleus 170 237 272 142 149 199 306 231 240 316 418 284 284 293 107 162 184 169 232 260 152 232 149
Nuclear Periphery 1 0 0 3 1 1 0 4 2 3 6 0 2 1 1 5 1 0 0 1 1 0 1
Nucleolus 14 2 0 0 0 0 1 8 8 10 8 0 0 2 1 0 3 4 2 2 9 16 17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 5 0 1 0 0 0 0 0 0 0
SpindlePole 0 1 9 0 1 2 6 3 5 5 7 0 2 2 0 3 0 4 0 1 3 2 3
Vac/Vac Membrane 2 8 0 1 2 1 10 4 2 2 7 2 8 16 11 11 12 13 10 16 17 29 42
Unique Cell Count 190 251 284 155 168 254 364 294 314 395 525 309 348 382 139 197 206 207 249 291 194 300 240
Labelled Cell Count 215 265 295 164 175 352 396 405 409 546 680 311 358 391 151 213 221 207 249 291 194 300 240


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 6.0 6.0 6.0 6.0 4.0 5.2 4.5 4.8 4.5 4.6 6.1 6.5 6.3 8.3 7.7 8.2 5.7 5.9 6.2
Std Deviation (1e-4) 0.9 0.9 1.3 1.6 1.7 1.2 1.7 1.6 1.7 1.4 1.2 1.2 1.8 1.8 2.2 1.8 2.5 1.6 1.5 1.9
Intensity Change (Log2) -0.01 0.0 -0.6 -0.22 -0.42 -0.32 -0.42 -0.4 0.02 0.12 0.07 0.46 0.36 0.44 -0.08 -0.05 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.0 2.0 2.9 2.5 1.7 2.9 0.7 1.1 1.9 5.0 5.4 4.3 2.0 1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.9 0 0 0 0 0 0 0 0 0 0
Nucleus -1.8 -2.9 -6.1 -4.8 -6.2 -6.7 -6.0 -6.2 -1.9 -5.4 -6.8 -5.9 -4.9 -2.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 -1.3 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 3.5 0 0 4.1


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Isy1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available