Standard name
Human Ortholog
Description Cytoplasmic RNA-binding protein; shuttles between nucleus and cytoplasm and is exported from the nucleus in an mRNA export-dependent manner; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.97 0.98 0.97 0.94 0.92 0.82 0.82 0.75 0.72 0.75 0.96 1.0 0.99 0.93 1.0 0.99 0.93 0.95 0.81 0.93 0.95 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.07 0.07 0.1 0.11 0.07 0 0 0 0 0.07 0.08 0 0 0.08 0 0 0.09
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.09 0.13 0.13 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 2 7 5 3 3 8 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 0 0 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 0 0 6 4 8 10 3 9 6 20 0 6 2 11 7 6 0 0 0 0 0 0
Cytoplasm 101 188 130 165 204 208 261 216 111 158 123 159 94 238 218 315 208 206 91 178 106 89 149 162
Endoplasmic Reticulum 1 4 3 3 4 7 13 19 9 20 19 15 4 1 2 14 15 16 0 2 10 2 4 17
Endosome 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 2 4 3 1 4 0 0 0 0 5 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1 4 5 12 27 23 32 0 0 0 9 1 1 0 0 1 0 0 1
Nucleus 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 1 0 0 1 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 3 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 1 1 4 7 14 3 9 11 3 0 1 0 5 0 2 0 1 3 1 0 6
Unique Cell Count 102 191 134 169 211 221 285 264 136 210 172 213 98 239 220 337 209 208 99 189 131 96 158 197
Labelled Cell Count 104 192 134 169 215 225 298 275 147 228 191 238 98 246 222 365 231 231 99 189 131 96 158 197


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 66.0 84.4 57.4 59.9 46.2 32.1 26.7 25.3 20.9 18.6 17.5 16.5 70.4 66.8 60.6 69.0 90.4 106.3 65.1 69.8 75.3
Std Deviation (1e-4) 18.3 19.0 18.2 14.0 10.0 8.0 6.0 5.9 5.5 4.8 4.6 3.6 20.2 13.9 15.2 26.2 29.4 28.4 23.1 16.7 20.4
Intensity Change (Log2) – – – 0.06 -0.31 -0.84 -1.1 -1.18 -1.46 -1.63 -1.71 -1.8 0.29 0.22 0.08 0.27 0.66 0.89 0.18 0.28 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 3.7 0 0 0 0 0 0
Cytoplasm 0.3 -0.2 -1.2 -2.1 -4.3 -4.1 -5.3 -5.8 -5.4 -0.5 2.1 1.5 -1.5 1.9 1.4
Endoplasmic Reticulum 0 0 0 1.2 2.0 1.7 2.6 3.0 2.0 0 0 0 0 2.0 2.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.5 4.3 4.4 4.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.3 0 0 2.5 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 49.0289 73.1651 62.2989 52.5297 66.0472 58.5268 54.1717 70.8643 64.1647 57.485 52.8161 65.1723 59.9468 78.637 70.0496 71.616 59.0514 70.6933
Actin 0.0017 0.0004 0.0045 0.0001 0.0001 0.0004 0.0207 0.0004 0.0109 0.0005 0.004 0.0009 0.0049 0.0005 0.0237 0.0005 0.0016 0.0029
Bud 0.001 0.0007 0.0012 0.0008 0.0003 0.0009 0.001 0.0008 0.0017 0.0007 0.0015 0.0013 0.001 0.0003 0.0009 0.001 0.0132 0.0014
Bud Neck 0.0006 0.0008 0.0007 0.0006 0.0008 0.0007 0.0006 0.0002 0.0004 0.0005 0.0049 0.0008 0.0006 0.0003 0.0006 0.0008 0.0004 0.001
Bud Periphery 0.001 0.0007 0.0009 0.0003 0.0001 0.0006 0.0011 0.0005 0.0007 0.0003 0.0009 0.0008 0.0007 0.0001 0.0004 0.0005 0.0048 0.0004
Bud Site 0.0008 0.0004 0.001 0.0001 0 0.0002 0.0021 0.0005 0.0037 0.0037 0.0495 0.0002 0.0006 0.0008 0.0015 0.0004 0.0025 0.0001
Cell Periphery 0.008 0.0036 0.0052 0.001 0.0005 0.0005 0.0033 0.0012 0.0005 0.0003 0.0006 0.0001 0.0039 0.0013 0.0004 0.0016 0.0009 0.0001
Cytoplasm 0.696 0.8814 0.7936 0.7187 0.8712 0.6431 0.8056 0.9735 0.9158 0.8015 0.7308 0.9114 0.8161 0.9774 0.895 0.9313 0.8351 0.9462
Cytoplasmic Foci 0.0184 0.0011 0.0037 0.0021 0.0019 0.0089 0.0143 0.0017 0.0096 0.0195 0.0267 0.0049 0.0144 0.0009 0.0153 0.0036 0.017 0.0049
Eisosomes 0.0001 0 0 0 0 0.0001 0.0024 0 0.0002 0 0 0 0.0001 0.0001 0.0002 0 0.0003 0
Endoplasmic Reticulum 0.0828 0.0325 0.0256 0.0045 0.0346 0.0798 0.0537 0.0112 0.0035 0.028 0.0042 0.028 0.0342 0.0018 0.0025 0.0025 0.0085 0.0077
Endosome 0.0178 0.0048 0.0091 0.0121 0.0117 0.0293 0.0129 0.0015 0.0131 0.0272 0.0544 0.0228 0.0185 0.0009 0.0088 0.0068 0.0171 0.0136
Golgi 0.003 0.0004 0.0009 0.0001 0.0002 0.0014 0.0052 0.0003 0.005 0.0029 0.006 0.0045 0.0032 0.0003 0.0116 0.0003 0.0018 0.0012
Lipid Particles 0.0117 0.0002 0.003 0.0003 0.0001 0.0069 0.0065 0.0002 0.001 0.0037 0.0054 0.0006 0.0057 0.0002 0.0019 0.001 0.0122 0
Mitochondria 0.0047 0.0006 0.0031 0.0002 0.0002 0.0086 0.0179 0.0003 0.007 0.0784 0.063 0.0024 0.0049 0.0005 0.0034 0.0003 0.0333 0.0013
None 0.0036 0.0002 0.0007 0.0003 0.0001 0.0039 0.0035 0.0014 0.0011 0.0012 0.0012 0.0005 0.0043 0.0002 0.0044 0.0002 0.0144 0.0004
Nuclear Periphery 0.022 0.0068 0.0088 0.0085 0.0036 0.0139 0.013 0.0006 0.0013 0.0019 0.0021 0.0035 0.0225 0.0012 0.0018 0.0038 0.0046 0.0071
Nucleolus 0.0034 0.0001 0.0004 0.0004 0 0.006 0.0003 0.0001 0.0012 0.0011 0.0005 0 0.0011 0.0037 0.0006 0.0001 0.0013 0
Nucleus 0.0057 0.0033 0.0036 0.0058 0.0026 0.005 0.0033 0.0007 0.0016 0.0024 0.0022 0.0023 0.0104 0.0022 0.0016 0.0037 0.0051 0.0052
Peroxisomes 0.003 0 0.0043 0 0 0.0014 0.0018 0 0.0007 0.0007 0.0007 0.0001 0.0014 0 0.0034 0.0001 0.0018 0.0001
Punctate Nuclear 0.0047 0 0.0001 0.0001 0 0.005 0.0033 0 0.0002 0.001 0.0005 0.0002 0.0036 0.0001 0.0003 0.0001 0.0007 0.0001
Vacuole 0.0888 0.0551 0.1101 0.1907 0.0512 0.1297 0.0206 0.0044 0.0159 0.02 0.0272 0.0102 0.0405 0.0062 0.0178 0.0341 0.0167 0.0038
Vacuole Periphery 0.0211 0.0069 0.0194 0.053 0.0208 0.0536 0.0069 0.0007 0.005 0.0043 0.0136 0.0048 0.0074 0.0008 0.004 0.0072 0.0068 0.0024

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 178.4049 218.4389 181.7215 171.1437 190.7483 145.349 159.5336 125.3408 118.7377 158.8158
Translational Efficiency 2.5771 1.9911 1.7842 1.1822 1.7322 2.2798 1.5463 1.6651 1.5888 1.7006

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘