Standard name
Description Nucleotide excision repair (NER) protein; binds damaged DNA during NER; binds DNA in an ATP-dependent manner (with Rad16p) during NER; required for repair of non-transcribed chromatin; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.69 0.72 0.85 0.86 0.69 0.54 0.44 – 0.28 0.27 0.24 0.27 0.84 0.84 0.79 0.06 0 0.05 0.49 0.43 0.51 0.66 0.53 0.62
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0 0 0 0 0.12 0.19 – 0.32 0.26 0.47 0.25 0 0 0 0.05 0.06 0 0.14 0.12 0.14 0 0 0
Nucleus 0.15 0.26 0.12 0.13 0.23 0.35 0.42 – 0.51 0.54 0.51 0.56 0.14 0.14 0.19 0.86 0.88 0.9 0.16 0.22 0.14 0.12 0.22 0.12
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Vac/Vac Membrane 0.08 0 0 0 0.07 0.07 0 – 0 0 0 0 0 0 0 0.05 0.05 0.07 0.11 0.13 0.11 0.08 0.11 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 1 2 1 0 0 1
Bud 2 0 0 0 0 1 0 – 3 3 5 4 1 0 0 0 1 1 0 0 1 0 0 3
Bud Neck 1 0 10 4 2 1 7 – 3 3 1 4 0 0 1 0 0 0 0 1 1 0 0 1
Bud Site 0 0 0 0 0 1 0 – 10 7 10 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 0 1 2 0 0 1 – 1 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0
Cytoplasm 128 41 185 230 180 194 150 – 99 87 103 110 113 117 126 14 6 10 112 42 115 27 48 79
Endoplasmic Reticulum 0 0 0 1 0 0 0 – 0 0 0 0 2 0 1 5 3 4 7 0 2 0 0 0
Endosome 5 0 1 0 1 0 0 – 3 3 1 0 0 1 0 2 5 0 2 1 1 0 0 1
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 2 1 0 0 0 0 0
Mitochondria 26 1 0 4 3 44 63 – 111 83 200 105 0 0 1 12 14 5 31 11 31 0 1 4
Nucleus 27 15 27 34 60 123 142 – 181 170 217 230 19 20 30 211 191 180 35 21 31 4 20 14
Nuclear Periphery 3 0 0 0 0 0 0 – 2 0 3 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 1 0 2 – 9 7 24 12 0 0 0 3 4 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 1 1 4 0 7 – 5 10 3 13 0 0 2 2 2 0 5 1 2 2 2 4
Vac/Vac Membrane 14 2 4 4 17 24 11 – 5 6 10 8 1 5 3 13 11 13 25 12 25 3 10 11
Unique Cell Count 185 57 218 268 260 356 338 352 317 426 413 135 140 159 244 216 200 231 98 228 41 91 129
Labelled Cell Count 212 59 230 280 268 388 383 432 379 577 494 136 143 164 264 237 215 231 98 228 41 91 129


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 5.0 5.1 4.7 5.3 4.3 4.4 – 4.2 4.4 3.9 4.2 5.1 5.3 5.3 8.0 7.6 7.2 – 4.8 5.5
Std Deviation (1e-4) 0.6 1.3 1.0 1.4 1.4 0.9 1.3 – 1.0 1.2 1.3 1.2 1.7 1.3 1.5 1.6 1.5 1.5 – 1.3 1.9
Intensity Change (Log2) – – – -0.1 0.05 -0.25 -0.19 – -0.27 -0.21 -0.37 -0.26 0.02 0.06 0.05 0.65 0.59 0.51 – -0.08 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 -2.7 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 -4.0 -7.5 -9.5 – -13.2 -13.1 -14.7 -13.9 -0.3 -0.3 -1.4 -17.1 -17.2 -16.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 3.3 3.8 0
Nucleus 0.1 3.0 5.9 7.4 – 9.4 9.7 9.5 10.5 0.5 0.5 1.7 15.9 15.8 15.9
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.5 2.6 1.0 – 0 0 0 0 0 0 0 2.0 1.9 2.4