Standard name
Human Ortholog
Description Nucleotide excision repair (NER) protein; binds damaged DNA during NER; binds DNA in an ATP-dependent manner (with Rad16p) during NER; required for repair of non-transcribed chromatin; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.69 0.72 0.85 0.86 0.69 0.54 0.44 0.28 0.27 0.24 0.27 0.84 0.84 0.79 0.06 0 0.05 0.49 0.43 0.51 0.66 0.53 0.62
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0 0 0 0 0.12 0.19 0.32 0.26 0.47 0.25 0 0 0 0.05 0.06 0 0.14 0.12 0.14 0 0 0
Nucleus 0.15 0.26 0.12 0.13 0.23 0.35 0.42 0.51 0.54 0.51 0.56 0.14 0.14 0.19 0.86 0.88 0.9 0.16 0.22 0.14 0.12 0.22 0.12
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Vac/Vac Membrane 0.08 0 0 0 0.07 0.07 0 0 0 0 0 0 0 0 0.05 0.05 0.07 0.11 0.13 0.11 0.08 0.11 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 1 0 0 1
Bud 2 0 0 0 0 1 0 3 3 5 4 1 0 0 0 1 1 0 0 1 0 0 3
Bud Neck 1 0 10 4 2 1 7 3 3 1 4 0 0 1 0 0 0 0 1 1 0 0 1
Bud Site 0 0 0 0 0 1 0 10 7 10 7 0 0 0 0 0 0
Cell Periphery 3 0 1 2 0 0 1 1 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0
Cytoplasm 128 41 185 230 180 194 150 99 87 103 110 113 117 126 14 6 10 112 42 115 27 48 79
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 0 0 2 0 1 5 3 4 7 0 2 0 0 0
Endosome 5 0 1 0 1 0 0 3 3 1 0 0 1 0 2 5 0 2 1 1 0 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1 0 0 0 0 0
Mitochondria 26 1 0 4 3 44 63 111 83 200 105 0 0 1 12 14 5 31 11 31 0 1 4
Nucleus 27 15 27 34 60 123 142 181 170 217 230 19 20 30 211 191 180 35 21 31 4 20 14
Nuclear Periphery 3 0 0 0 0 0 0 2 0 3 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 1 0 2 9 7 24 12 0 0 0 3 4 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 1 1 4 0 7 5 10 3 13 0 0 2 2 2 0 5 1 2 2 2 4
Vac/Vac Membrane 14 2 4 4 17 24 11 5 6 10 8 1 5 3 13 11 13 25 12 25 3 10 11
Unique Cell Count 185 57 218 268 260 356 338 352 317 426 413 135 140 159 244 216 200 231 98 228 41 91 129
Labelled Cell Count 212 59 230 280 268 388 383 432 379 577 494 136 143 164 264 237 215 231 98 228 41 91 129


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 5.0 5.1 4.7 5.3 4.3 4.4 4.2 4.4 3.9 4.2 5.1 5.3 5.3 8.0 7.6 7.2 4.8 5.5
Std Deviation (1e-4) 0.6 1.3 1.0 1.4 1.4 0.9 1.3 1.0 1.2 1.3 1.2 1.7 1.3 1.5 1.6 1.5 1.5 1.3 1.9
Intensity Change (Log2) -0.1 0.05 -0.25 -0.19 -0.27 -0.21 -0.37 -0.26 0.02 0.06 0.05 0.65 0.59 0.51 -0.08 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 -2.7 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 -4.0 -7.5 -9.5 -13.2 -13.1 -14.7 -13.9 -0.3 -0.3 -1.4 -17.1 -17.2 -16.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 3.3 3.8 0
Nucleus 0.1 3.0 5.9 7.4 9.4 9.7 9.5 10.5 0.5 0.5 1.7 15.9 15.8 15.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.5 2.6 1.0 0 0 0 0 0 0 0 2.0 1.9 2.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5694 -0.9179 -0.7958 -0.9099 -1.2764 -0.7836 -0.9765 -0.5805 -0.9215 -0.9372 -1.3287 -0.9759 -0.2467 -0.9938 -0.8097 -1.1097 -0.7618 -1.0241
Actin 0.0473 0.0002 0.0016 0.0016 0.0007 0.0015 0.0131 0.0001 0.0131 0.0008 0.0011 0.0007 0.0542 0.002 0.0088 0.0319 0.1215 0.0005
Bud 0.0007 0.0023 0.0002 0.0001 0.0019 0.0001 0.0012 0.0004 0.0009 0.0027 0.0001 0.0013 0.003 0.001 0.0007 0.0117 0.001 0.0002
Bud Neck 0.0056 0.0004 0.0002 0.0002 0.0009 0.0016 0.0005 0.0008 0.0006 0.0014 0.0003 0.0011 0.0088 0.0003 0.0009 0.0019 0.0005 0.0011
Bud Periphery 0.0016 0.0037 0.0003 0.0001 0.0013 0.0002 0.001 0.0002 0.0025 0.0023 0.0001 0.0052 0.0061 0.0029 0.0008 0.0062 0.0019 0.0002
Bud Site 0.003 0.0061 0.0022 0.0001 0.0006 0.0002 0.0043 0.0087 0.0014 0.0332 0.0001 0.0005 0.0112 0.0203 0.013 0.0037 0.0014 0.0001
Cell Periphery 0.0002 0.0001 0 0 0.0001 0 0.0003 0.0002 0.0001 0.0003 0 0.0001 0.0009 0.0002 0.0003 0.0004 0.0002 0
Cytoplasm 0.0414 0.0435 0.0393 0.0339 0.0498 0.0671 0.0235 0.0174 0.044 0.0379 0.029 0.055 0.0417 0.067 0.0491 0.061 0.0431 0.1167
Cytoplasmic Foci 0.0196 0.0105 0.004 0.0144 0.0093 0.0148 0.0117 0.0095 0.0099 0.0288 0.0026 0.0072 0.0421 0.0046 0.0133 0.0175 0.0112 0.0172
Eisosomes 0.0009 0.0001 0.0002 0.0001 0.0001 0.0001 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001 0.0006 0.0002 0.0002 0.0004 0.0005 0.0001
Endoplasmic Reticulum 0.0038 0.0011 0.0011 0.0008 0.0028 0.0015 0.0025 0.0003 0.0018 0.0008 0.0004 0.0004 0.0052 0.0007 0.0013 0.0025 0.0026 0.001
Endosome 0.0097 0.0011 0.001 0.0184 0.0029 0.0021 0.0215 0.0008 0.0166 0.0056 0.0024 0.0055 0.0302 0.0007 0.0115 0.0091 0.0066 0.003
Golgi 0.0052 0.0001 0.0001 0.0004 0.0002 0.0002 0.0063 0.0003 0.0055 0.0024 0.002 0.0007 0.0093 0.0002 0.0048 0.006 0.0177 0.0002
Lipid Particles 0.0092 0.0007 0.0004 0.0016 0.0006 0.0006 0.0067 0.0063 0.0037 0.0102 0.0045 0.0093 0.0308 0.0003 0.0046 0.0082 0.0568 0.0007
Mitochondria 0.0021 0.0006 0.0004 0.0005 0.0013 0.0005 0.0352 0.0004 0.0016 0.002 0.0788 0.0015 0.0148 0.0009 0.0109 0.0107 0.012 0.0006
None 0.6718 0.8021 0.8318 0.7207 0.7252 0.7657 0.7534 0.8222 0.7514 0.6282 0.6707 0.7536 0.5437 0.8124 0.7393 0.7393 0.5709 0.6433
Nuclear Periphery 0.0093 0.0038 0.0022 0.0048 0.0119 0.0041 0.0101 0.0016 0.0027 0.0026 0.0022 0.0011 0.0126 0.0008 0.0034 0.0083 0.0018 0.0027
Nucleolus 0.0021 0.0029 0.0015 0.003 0.0047 0.0021 0.0038 0.0057 0.0034 0.0049 0.0033 0.0027 0.0053 0.0007 0.0016 0.0039 0.0044 0.0022
Nucleus 0.112 0.1048 0.0976 0.1726 0.1389 0.1233 0.0876 0.1049 0.1208 0.2021 0.1829 0.1342 0.1238 0.0803 0.1064 0.0589 0.1019 0.1848
Peroxisomes 0.0099 0.0005 0.0003 0.0033 0.0004 0.0011 0.0055 0.0005 0.005 0.0108 0.0056 0.0013 0.0346 0.0012 0.013 0.007 0.0397 0.0008
Punctate Nuclear 0.0425 0.0145 0.0149 0.0199 0.0432 0.0122 0.0085 0.0188 0.0138 0.0184 0.0134 0.0165 0.0105 0.0024 0.0122 0.0063 0.0025 0.0225
Vacuole 0.0015 0.0008 0.0004 0.0027 0.0026 0.0007 0.0017 0.0008 0.001 0.0041 0.0002 0.0018 0.008 0.0011 0.0036 0.0038 0.0013 0.0016
Vacuole Periphery 0.0006 0.0002 0.0001 0.0007 0.0007 0.0002 0.0012 0.0001 0.0002 0.0005 0.0002 0.0002 0.0026 0.0001 0.0003 0.0013 0.0005 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.473 5.809 6.0348 9.808 7.5827 5.8452 9.6913 10.9016 11.0707 10.8481
Translational Efficiency 1.0364 1.6624 1.2989 0.9118 1.2055 2.0837 1.1621 1.0087 1.0473 1.0746

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
943 1437 420 664 1713 1838 285 762 2656 3275 705 1426

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 612.77 691.19 951.92 824.13 663.96 687.24 896.59 798.69 645.79 688.97 929.55 810.54
Standard Deviation 85.56 107.49 109.20 106.88 75.15 90.68 93.99 112.99 82.71 98.43 106.83 110.91
Intensity Change Log 2 0.173737 0.635495 0.427526 0.049718 0.433352 0.266539 0.110573 0.533909 0.346050

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000182 0.000205 0.000902 0.000609 0.000059 0.000288 0.000258 0.000409 0.000103 0.000251 0.000642 0.000502
Bud Neck 0.002556 0.024643 0.005251 0.013813 0.011071 0.033530 0.001131 0.006798 0.008048 0.029631 0.003586 0.010065
Bud Site 0.001639 0.005712 0.010498 0.019085 0.002153 0.014047 0.000934 0.021086 0.001970 0.010390 0.006632 0.020155
Cell Periphery 0.000291 0.000181 0.000229 0.000205 0.000126 0.000252 0.000150 0.000228 0.000185 0.000221 0.000197 0.000217
Cytoplasm 0.073762 0.387368* 0.099236 0.275877* 0.476902* 0.355885* 0.169538* 0.360283* 0.333769* 0.369699* 0.127656* 0.320980*
Cytoplasmic Foci 0.010735 0.186676* 0.007407 0.009769 0.171604 0.182800* 0.028350 0.010445 0.114488 0.184501* 0.015873 0.010130
Eisosomes 0.000041 0.000134 0.000098 0.000056 0.000096 0.000138 0.000090 0.000052 0.000077 0.000136 0.000095 0.000054
Endoplasmic Reticulum 0.001677 0.000873 0.009853 0.005537 0.000645 0.000897 0.003884 0.003807 0.001012 0.000886 0.007440 0.004613
Endosome 0.000691 0.011488 0.003897 0.003114 0.005742 0.022915 0.003459 0.004785 0.003949 0.017901 0.003720 0.004006
Golgi 0.000104 0.001803 0.000120 0.006503 0.001355 0.004248 0.000073 0.001943 0.000911 0.003175 0.000101 0.004067
Lipid Particles 0.002659 0.008473 0.003534 0.001691 0.006879 0.010471 0.004068 0.001379 0.005381 0.009594 0.003750 0.001524
Mitochondria 0.001219 0.008544 0.000988 0.001516 0.002581 0.017223 0.000359 0.001998 0.002097 0.013415 0.000734 0.001773
Mitotic Spindle 0.001193 0.002084 0.001330 0.011520 0.003751 0.008238 0.001149 0.029307 0.002843 0.005538 0.001257 0.021024
None 0.024557 0.011483 0.002911 0.006889 0.004548 0.006811 0.004335 0.013174 0.011652 0.008861 0.003487 0.010247
Nuclear Periphery 0.001055 0.000649 0.002310 0.002202 0.000584 0.000808 0.001708 0.001819 0.000751 0.000738 0.002066 0.001997
Nuclear Periphery Foci 0.000517 0.000467 0.001753 0.001447 0.000333 0.000301 0.001055 0.000896 0.000398 0.000374 0.001471 0.001152
Nucleolus 0.013046 0.003520 0.002378 0.000551 0.001412 0.004616 0.001139 0.001002 0.005542 0.004135 0.001877 0.000792
Nucleus 0.860705* 0.291500* 0.781931* 0.564821* 0.278464* 0.268657* 0.720171* 0.461366* 0.485186* 0.278680* 0.756964* 0.509539*
Peroxisomes 0.000659 0.010953 0.000569 0.000551 0.003301 0.015260 0.000480 0.001161 0.002363 0.013370 0.000533 0.000877
Vacuole 0.002514 0.042214 0.064478 0.071461 0.027918 0.049999 0.057480 0.076310 0.018899 0.046583 0.061649 0.074052
Vacuole Periphery 0.000199 0.001030 0.000329 0.002782 0.000474 0.002615 0.000189 0.001752 0.000376 0.001919 0.000273 0.002231

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.28 -8.66 -4.22 -5.17 4.30 -3.84 -8.08 -11.73 -3.10 -2.43 -3.33 -11.83 -8.98 -5.82 4.00
Bud Neck -10.85 -5.08 -5.19 5.45 -2.44 -10.06 7.62 2.30 12.66 -7.23 -13.57 3.74 -0.93 12.74 -4.75
Bud Site -3.81 -2.45 -3.96 -2.44 -0.48 -8.31 2.23 -4.99 0.15 -5.36 -9.25 -2.26 -6.37 -1.28 -2.51
Cell Periphery 2.87 0.98 2.90 0.00 3.69 -4.18 -1.95 -2.24 2.54 -0.67 -1.57 -1.55 0.59 2.36 2.66
Cytoplasm -33.79* -1.66 -12.26 15.93 -9.98 13.05 24.02 18.94 8.09 -7.06 -4.44 25.29 11.55 16.37 -12.83
Cytoplasmic Foci -30.85* 2.50 2.18 32.34 -0.44 -1.67 25.51 34.69 35.61 3.88 -14.19 27.31 31.09 48.12 3.23
Eisosomes -10.86 -9.25 -6.87 6.45 4.69 -4.74 -1.43 5.40 7.78 4.71 -9.67 -6.63 -0.01 10.02 6.90
Endoplasmic Reticulum 4.59 -14.72 -13.45 -15.45 5.05 -2.08 -9.59 -14.60 -13.74 -0.46 1.24 -17.20 -19.88 -20.46 5.07
Endosome -11.81 -10.09 -6.75 9.43 4.42 -11.81 3.45 3.85 13.40 0.96 -15.12 -0.84 2.54 16.21 3.44
Golgi -9.47 -2.55 -1.56 -0.20 -1.52 -4.82 3.90 1.18 5.18 -1.28 -6.25 3.74 -0.60 2.65 -1.89
Lipid Particles -8.25 -2.49 2.25 10.76 5.88 -3.77 4.01 8.59 12.11 5.05 -6.61 3.04 8.75 16.03 7.92
Mitochondria -5.99 1.00 0.47 6.07 -0.46 -9.14 4.06 1.74 10.21 -3.61 -10.64 3.69 1.96 11.87 -2.86
Mitotic Spindle -0.70 -1.34 -4.05 -3.88 -2.82 -2.32 1.84 -4.20 -3.11 -5.07 -2.09 0.08 -5.61 -4.72 -5.38
None 4.32 8.43 7.32 3.41 -3.93 -2.59 1.05 -2.65 -0.89 -3.19 2.23 8.06 4.09 1.94 -4.81
Nuclear Periphery 3.74 -12.05 -13.60 -16.64 -3.05 -2.96 -12.73 -14.59 -12.70 -5.44 -0.11 -18.61 -20.58 -20.13 -5.37
Nuclear Periphery Foci 0.36 -6.17 -4.16 -5.77 2.31 0.94 -6.46 -4.69 -4.94 -0.35 0.54 -9.14 -6.72 -7.57 2.07
Nucleolus 8.86 10.79 12.53 7.53 6.66 -6.36 1.67 1.96 8.23 0.53 2.76 9.20 11.76 11.09 5.69
Nucleus 52.49* 7.48 20.71 -20.93 10.07 1.05 -22.99 -18.72 -18.53 8.72 23.36 -19.39 -5.62 -27.73 13.95
Peroxisomes -9.67 1.27 2.21 9.96 1.33 -9.99 5.51 4.86 13.41 -0.25 -13.19 5.53 5.29 16.72 0.37
Vacuole -17.18 -12.05 -18.46 -12.70 -4.44 -8.77 -7.28 -15.01 -11.85 -3.56 -15.63 -12.86 -22.89 -17.28 -5.69
Vacuole Periphery -10.38 -4.15 -1.35 0.14 -1.10 -9.13 5.95 -0.58 4.56 -1.38 -11.21 2.85 -1.33 3.20 -1.65