Standard name | |
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Human Ortholog | |
Description | Microtubule-associated protein; component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 1 | 0 | 1 | 1 | 7 | 3 | 0 | 1 | 0 | 0 | 3 | 0 | 3 | 0 | 1 | 0 | 1 | 0 | 2 | 4 | 11 | 10 |
Bud | 1 | 1 | 4 | 1 | 9 | 10 | 45 | 36 | 40 | 35 | 22 | 31 | 1 | 0 | 1 | 1 | 2 | 1 | 2 | 1 | 1 | 9 | 16 | 11 |
Bud Neck | 0 | 0 | 3 | 3 | 3 | 4 | 6 | 9 | 9 | 4 | 3 | 4 | 2 | 1 | 1 | 2 | 1 | 0 | 1 | 1 | 1 | 10 | 8 | 7 |
Bud Site | 11 | 0 | 12 | 14 | 14 | 21 | 23 | 21 | 31 | 52 | 33 | 41 | 3 | 3 | 3 | 4 | 3 | 2 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 0 | 4 | 6 | 15 | 12 | 19 | 11 | 8 | 13 | 6 | 5 | 11 | 22 | 27 | 11 | 26 | 20 | 0 | 0 | 0 | 1 | 2 | 2 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 8 | 7 | 4 | 0 | 0 | 1 | 0 | 0 | 0 |
Endosome | 23 | 9 | 108 | 46 | 51 | 34 | 16 | 65 | 21 | 13 | 9 | 19 | 64 | 111 | 107 | 111 | 96 | 71 | 45 | 23 | 96 | 32 | 52 | 61 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 3 | 0 | 4 | 1 | 4 | 1 |
Mitochondria | 89 | 15 | 68 | 111 | 119 | 214 | 291 | 243 | 353 | 427 | 372 | 419 | 1 | 0 | 4 | 48 | 12 | 10 | 8 | 5 | 18 | 14 | 21 | 23 |
Nucleus | 4 | 2 | 3 | 3 | 11 | 13 | 9 | 13 | 13 | 14 | 31 | 17 | 5 | 4 | 6 | 6 | 7 | 6 | 0 | 0 | 0 | 2 | 1 | 3 |
Nuclear Periphery | 2 | 0 | 0 | 1 | 2 | 4 | 9 | 16 | 16 | 11 | 15 | 17 | 0 | 0 | 1 | 4 | 3 | 3 | 0 | 0 | 0 | 1 | 1 | 0 |
Nucleolus | 56 | 17 | 65 | 67 | 94 | 98 | 138 | 145 | 169 | 179 | 159 | 186 | 57 | 79 | 71 | 154 | 112 | 87 | 5 | 3 | 6 | 9 | 10 | 6 |
Peroxisomes | 16 | 0 | 91 | 49 | 52 | 53 | 80 | 126 | 31 | 46 | 21 | 68 | 23 | 38 | 66 | 51 | 48 | 30 | 30 | 6 | 71 | 37 | 101 | 103 |
SpindlePole | 55 | 9 | 168 | 86 | 132 | 94 | 133 | 228 | 169 | 154 | 94 | 134 | 157 | 231 | 192 | 178 | 135 | 103 | 67 | 24 | 130 | 60 | 125 | 183 |
Vac/Vac Membrane | 2 | 3 | 9 | 4 | 6 | 8 | 2 | 4 | 9 | 6 | 8 | 7 | 7 | 5 | 13 | 16 | 12 | 16 | 0 | 1 | 0 | 2 | 5 | 3 |
Unique Cell Count | 172 | 40 | 329 | 248 | 337 | 384 | 529 | 623 | 638 | 691 | 551 | 684 | 226 | 338 | 332 | 354 | 290 | 216 | 187 | 76 | 365 | 202 | 378 | 442 |
Labelled Cell Count | 266 | 56 | 536 | 391 | 509 | 567 | 778 | 920 | 869 | 956 | 773 | 950 | 334 | 495 | 497 | 596 | 466 | 355 | 187 | 76 | 365 | 202 | 378 | 442 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 6.3 | 5.4 | 5.0 | 5.3 | 4.5 | 4.5 | 4.6 | 4.3 | 4.3 | 4.0 | 4.2 | 7.3 | 7.2 | 7.0 | 8.3 | 8.3 | 8.7 | 6.9 | 6.9 | 7.0 |
Std Deviation (1e-4) | 0.7 | 1.1 | 0.8 | 1.0 | 1.5 | 0.9 | 1.4 | 1.0 | 0.9 | 1.1 | 0.9 | 1.0 | 1.2 | 1.3 | 1.2 | 1.7 | 1.7 | 1.7 | 1.2 | 1.2 | 1.1 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.03 | -0.25 | -0.25 | -0.24 | -0.32 | -0.34 | -0.43 | -0.35 | 0.44 | 0.42 | 0.36 | 0.61 | 0.62 | 0.69 | 0.36 | 0.34 | 0.37 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 1.1 | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.7 | -2.4 | -2.4 | -2.2 | -2.1 | -2.0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 2.5 | 1.7 | 2.1 | 0.6 | 0 | 0.8 | 0 | 0 | 2.6 | 3.5 | 4.2 | 1.7 | 4.5 | 4.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -3.8 | -5.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.1 | 0 | -0.2 | -0.4 | 0.1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 6.2 | 4.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -7.1 | -8.8 | -8.0 | -2.5 | -6.1 | -5.2 |
Nucleus | 0 | 2.1 | 2.2 | 0 | 1.3 | 1.3 | 1.3 | 3.5 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.1 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 1.1 | 0.5 | 6.6 | 5.2 | 5.2 |
Peroxisomes | -2.2 | -3.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.0 | -5.4 | -2.4 | -4.3 | -3.3 | -3.8 |
SpindlePole | -3.9 | -3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.3 | 4.6 | 1.7 | -0.2 | -1.1 | -0.8 |
Vacuole | -0.9 | -0.8 | -0.6 | 0 | 0 | -1.4 | 0 | -1.3 | -2.0 | 0.3 | -1.1 | 0.8 | 1.2 | 1.0 | 2.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.425 | 1.9761 | 1.9181 | 1.2859 | 1.2153 | 1.6268 | 1.5083 | 1.8212 | 1.641 | 1.594 | 1.2783 | 1.4509 | 0.3122 | 0.8186 | 0.5227 | 0.2726 | 0.5981 | 0.2134 |
Actin | 0.01 | 0.0043 | 0.0095 | 0.0057 | 0.0016 | 0.0092 | 0.034 | 0.0063 | 0.005 | 0.0027 | 0.0449 | 0.0097 | 0.0159 | 0.0021 | 0.0019 | 0.0022 | 0.0064 | 0.0062 |
Bud | 0.0002 | 0.0025 | 0.0005 | 0.0006 | 0.0001 | 0.0004 | 0.0007 | 0.0007 | 0.0002 | 0.0002 | 0.0012 | 0.0003 | 0.0003 | 0.001 | 0.0001 | 0.0004 | 0.0002 | 0.0005 |
Bud Neck | 0.0164 | 0.007 | 0.0179 | 0.0572 | 0.0001 | 0.006 | 0.0018 | 0.0032 | 0.0014 | 0.0088 | 0.0089 | 0.0052 | 0.0009 | 0.013 | 0.0011 | 0.0215 | 0.0002 | 0.0038 |
Bud Periphery | 0.0002 | 0.0028 | 0.0007 | 0.0009 | 0.0001 | 0.001 | 0.0013 | 0.0011 | 0.0004 | 0.0006 | 0.0017 | 0.0007 | 0.0008 | 0.0016 | 0.0002 | 0.0011 | 0.0004 | 0.0008 |
Bud Site | 0.0032 | 0.0468 | 0.0058 | 0.0046 | 0.0002 | 0.0019 | 0.0036 | 0.0218 | 0.004 | 0.0076 | 0.0064 | 0.0012 | 0.0017 | 0.0146 | 0.002 | 0.0034 | 0.0006 | 0.0027 |
Cell Periphery | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0004 | 0 | 0.0001 |
Cytoplasm | 0.0065 | 0.0291 | 0.0003 | 0.0026 | 0.0002 | 0.012 | 0.0036 | 0.0027 | 0.0005 | 0.0004 | 0.0051 | 0.0016 | 0.0022 | 0.0079 | 0.0002 | 0.0002 | 0.0003 | 0.0041 |
Cytoplasmic Foci | 0.2054 | 0.08 | 0.0511 | 0.1608 | 0.1221 | 0.1285 | 0.1409 | 0.1026 | 0.0514 | 0.0833 | 0.0752 | 0.0851 | 0.1565 | 0.087 | 0.0498 | 0.0844 | 0.071 | 0.0847 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0011 | 0.0029 | 0.0001 | 0.0004 | 0.0001 | 0.0006 | 0.0017 | 0.0005 | 0.0002 | 0.0005 | 0.0014 | 0.0007 | 0.0013 | 0.0017 | 0 | 0.0001 | 0.0002 | 0.0011 |
Endosome | 0.0221 | 0.0208 | 0.003 | 0.014 | 0.007 | 0.0209 | 0.0294 | 0.0138 | 0.0059 | 0.0076 | 0.0501 | 0.0197 | 0.0189 | 0.0243 | 0.0027 | 0.0056 | 0.0155 | 0.0193 |
Golgi | 0.0131 | 0.0082 | 0.0044 | 0.0138 | 0.0164 | 0.0137 | 0.022 | 0.011 | 0.0052 | 0.0136 | 0.0306 | 0.0189 | 0.0129 | 0.009 | 0.0062 | 0.0102 | 0.0222 | 0.0174 |
Lipid Particles | 0.2851 | 0.1024 | 0.0722 | 0.1511 | 0.4361 | 0.2149 | 0.2061 | 0.1033 | 0.0661 | 0.1961 | 0.2646 | 0.2217 | 0.2298 | 0.0909 | 0.0781 | 0.1889 | 0.4225 | 0.202 |
Mitochondria | 0.0079 | 0.0026 | 0.0009 | 0.0035 | 0.0115 | 0.0051 | 0.0115 | 0.0084 | 0.003 | 0.0149 | 0.023 | 0.0105 | 0.0031 | 0.002 | 0.0005 | 0.0131 | 0.0259 | 0.0114 |
None | 0.0056 | 0.0115 | 0.0006 | 0.0017 | 0.0003 | 0.0014 | 0.0027 | 0.0007 | 0.0005 | 0.0004 | 0.001 | 0.0006 | 0.0039 | 0.0014 | 0.0002 | 0.0005 | 0.0007 | 0.0035 |
Nuclear Periphery | 0.006 | 0.0145 | 0.0018 | 0.0036 | 0.0035 | 0.0088 | 0.0145 | 0.0053 | 0.0026 | 0.0054 | 0.004 | 0.0202 | 0.0105 | 0.0264 | 0.0015 | 0.0036 | 0.0046 | 0.0156 |
Nucleolus | 0.0113 | 0.0977 | 0.1196 | 0.0588 | 0.0026 | 0.0187 | 0.0252 | 0.0637 | 0.0932 | 0.0479 | 0.0094 | 0.0296 | 0.0257 | 0.1469 | 0.0724 | 0.1171 | 0.0023 | 0.0455 |
Nucleus | 0.0057 | 0.0906 | 0.0357 | 0.0792 | 0.0004 | 0.0177 | 0.023 | 0.0277 | 0.0089 | 0.0045 | 0.0027 | 0.0101 | 0.0285 | 0.0782 | 0.0142 | 0.0141 | 0.0009 | 0.0198 |
Peroxisomes | 0.0926 | 0.0676 | 0.0127 | 0.1697 | 0.2876 | 0.1333 | 0.1211 | 0.1119 | 0.019 | 0.1063 | 0.3 | 0.1417 | 0.076 | 0.0401 | 0.0306 | 0.0955 | 0.3701 | 0.1357 |
Punctate Nuclear | 0.3062 | 0.4033 | 0.6618 | 0.2691 | 0.1093 | 0.4017 | 0.3539 | 0.5125 | 0.7316 | 0.4978 | 0.1651 | 0.4202 | 0.4075 | 0.4459 | 0.7376 | 0.4359 | 0.0543 | 0.4233 |
Vacuole | 0.0008 | 0.004 | 0.0008 | 0.002 | 0.0003 | 0.0032 | 0.0013 | 0.0019 | 0.0004 | 0.0006 | 0.0028 | 0.0014 | 0.0025 | 0.0041 | 0.0002 | 0.0014 | 0.0006 | 0.0015 |
Vacuole Periphery | 0.0005 | 0.0008 | 0.0002 | 0.0004 | 0.0005 | 0.0007 | 0.0011 | 0.0006 | 0.0002 | 0.0005 | 0.0013 | 0.0008 | 0.0008 | 0.0015 | 0.0001 | 0.0004 | 0.0009 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.9273 | 11.929 | 12.7245 | 13.0002 | 15.6794 | 14.1704 | 13.946 | 15.518 | 13.0136 | 15.5192 |
Translational Efficiency | 2.0096 | 1.4185 | 1.0932 | 1.0947 | 1.0118 | 1.5708 | 0.9417 | 1.146 | 1.1167 | 1.0206 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
90 | 254 | 2121 | 791 | 1001 | 2274 | 157 | 1567 | 1091 | 2528 | 2278 | 2358 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 826.94 | 883.83 | 932.27 | 945.46 | 738.18 | 825.77 | 1041.51 | 955.53 | 745.50 | 831.60 | 939.80 | 952.15 |
Standard Deviation | 118.93 | 110.87 | 117.02 | 127.84 | 105.61 | 107.12 | 122.37 | 127.58 | 109.53 | 108.91 | 120.61 | 127.76 |
Intensity Change Log 2 | — | 0.095986 | 0.172965 | 0.193234 | — | 0.161767 | 0.496632 | 0.372329 | — | 0.127386 | 0.334688 | 0.280478 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002688 | 0.001260 | 0.001421 | 0.009414 | 0.001797 | 0.002229 | 0.004420 | 0.006846 | 0.001871 | 0.002131 | 0.001627 | 0.007707 |
Bud Neck | 0.011940 | 0.004976 | 0.001886 | 0.004486 | 0.005351 | 0.005527 | 0.002127 | 0.003407 | 0.005894 | 0.005472 | 0.001902 | 0.003769 |
Bud Site | 0.026107 | 0.006117 | 0.005285 | 0.025533 | 0.006265 | 0.007821 | 0.012054 | 0.019986 | 0.007902 | 0.007649 | 0.005752 | 0.021847 |
Cell Periphery | 0.000303 | 0.000102 | 0.000029 | 0.000065 | 0.000213 | 0.000114 | 0.000043 | 0.000048 | 0.000221 | 0.000112 | 0.000030 | 0.000054 |
Cytoplasm | 0.012041 | 0.007102 | 0.002163 | 0.005640 | 0.015663 | 0.007371 | 0.001117 | 0.005607 | 0.015364 | 0.007344 | 0.002091 | 0.005618 |
Cytoplasmic Foci | 0.078479 | 0.067049 | 0.009371 | 0.021938 | 0.108908 | 0.089319 | 0.009426 | 0.018771 | 0.106398 | 0.087081 | 0.009375 | 0.019833 |
Eisosomes | 0.000096 | 0.000058 | 0.000010 | 0.000024 | 0.000096 | 0.000052 | 0.000021 | 0.000020 | 0.000096 | 0.000052 | 0.000011 | 0.000021 |
Endoplasmic Reticulum | 0.007762 | 0.001099 | 0.000279 | 0.001082 | 0.001961 | 0.000835 | 0.000737 | 0.000433 | 0.002439 | 0.000862 | 0.000310 | 0.000650 |
Endosome | 0.040691 | 0.024820 | 0.003598 | 0.009837 | 0.024967 | 0.029048 | 0.007121 | 0.010076 | 0.026264 | 0.028623 | 0.003840 | 0.009996 |
Golgi | 0.035598 | 0.013893 | 0.001198 | 0.005308 | 0.023313 | 0.021794 | 0.001589 | 0.004556 | 0.024326 | 0.021000 | 0.001225 | 0.004809 |
Lipid Particles | 0.094979 | 0.035059 | 0.008765 | 0.011859 | 0.152492* | 0.093800 | 0.011809 | 0.007498 | 0.147748* | 0.087898 | 0.008974 | 0.008961 |
Mitochondria | 0.017400 | 0.009796 | 0.001766 | 0.007710 | 0.009289 | 0.015009 | 0.000417 | 0.005563 | 0.009958 | 0.014485 | 0.001673 | 0.006283 |
Mitotic Spindle | 0.506713* | 0.720987* | 0.936945* | 0.848892* | 0.431265* | 0.580552* | 0.893186* | 0.865134* | 0.437489* | 0.594662* | 0.933930* | 0.859685* |
None | 0.002820 | 0.002777 | 0.000273 | 0.000180 | 0.006208 | 0.000857 | 0.000084 | 0.000093 | 0.005928 | 0.001050 | 0.000260 | 0.000122 |
Nuclear Periphery | 0.000981 | 0.000499 | 0.000322 | 0.000489 | 0.000683 | 0.000594 | 0.000309 | 0.000384 | 0.000707 | 0.000584 | 0.000321 | 0.000420 |
Nuclear Periphery Foci | 0.032131 | 0.020772 | 0.004757 | 0.004150 | 0.058291 | 0.019132 | 0.010091 | 0.003047 | 0.056133 | 0.019297 | 0.005124 | 0.003417 |
Nucleolus | 0.028414 | 0.010126 | 0.002023 | 0.001246 | 0.027836 | 0.011333 | 0.003185 | 0.000856 | 0.027883 | 0.011211 | 0.002103 | 0.000987 |
Nucleus | 0.024875 | 0.007360 | 0.001291 | 0.002097 | 0.014313 | 0.004629 | 0.005171 | 0.000644 | 0.015185 | 0.004903 | 0.001558 | 0.001132 |
Peroxisomes | 0.068137 | 0.063292 | 0.017510 | 0.032694 | 0.108674* | 0.106666* | 0.035423 | 0.041736 | 0.105330* | 0.102308* | 0.018744 | 0.038703 |
Vacuole | 0.005266 | 0.002190 | 0.001023 | 0.006218 | 0.001971 | 0.002567 | 0.001603 | 0.004798 | 0.002243 | 0.002529 | 0.001063 | 0.005274 |
Vacuole Periphery | 0.002579 | 0.000668 | 0.000085 | 0.001137 | 0.000443 | 0.000752 | 0.000065 | 0.000498 | 0.000619 | 0.000743 | 0.000084 | 0.000712 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.23 | 1.76 | -3.37 | -4.16 | -4.03 | -0.81 | -1.39 | -5.17 | -4.68 | -0.82 | -0.49 | 0.12 | -6.31 | -5.94 | -6.24 |
Bud Neck | 2.18 | 3.18 | 1.92 | -0.84 | -5.12 | -0.85 | 1.81 | 1.43 | 2.31 | -0.80 | -0.02 | 5.45 | 1.41 | 1.50 | -5.38 |
Bud Site | 1.79 | 1.80 | -0.57 | -6.42 | -6.63 | -1.32 | -2.18 | -8.13 | -7.43 | -1.70 | 0.16 | 0.31 | -8.85 | -9.64 | -9.32 |
Cell Periphery | 2.41 | 3.41 | 2.90 | 1.94 | -3.81 | 6.16 | 7.40 | 10.93 | 6.94 | 1.55 | 6.65 | 11.78 | 10.86 | 6.61 | -2.76 |
Cytoplasm | 1.10 | 2.53 | 0.95 | -0.32 | -3.02 | 3.42 | 5.09 | 3.09 | -0.36 | -3.08 | 3.55 | 6.01 | 3.32 | -0.37 | -4.79 |
Cytoplasmic Foci | 0.19 | 5.04 | 3.86 | 5.08 | -4.88 | 3.35 | 18.03 | 16.17 | 19.89 | -4.37 | 3.35 | 19.58 | 16.61 | 20.67 | -7.25 |
Eisosomes | 2.46 | 7.09 | 5.75 | 4.23 | -4.70 | 9.93 | 7.52 | 17.58 | 12.93 | 2.68 | 10.19 | 20.02 | 18.13 | 13.12 | -3.83 |
Endoplasmic Reticulum | 1.83 | 2.06 | 1.74 | -0.78 | -3.06 | 8.44 | 0.08 | 10.86 | 4.92 | 2.69 | 5.27 | 6.33 | 5.33 | 0.93 | -1.67 |
Endosome | 1.30 | 3.56 | 2.90 | 3.63 | -4.56 | -2.17 | 5.27 | 6.29 | 9.78 | -1.23 | -1.49 | 11.05 | 7.17 | 10.82 | -7.03 |
Golgi | 3.10 | 5.44 | 4.73 | 3.36 | -3.30 | 0.58 | 12.57 | 10.35 | 11.89 | -2.70 | 1.51 | 14.42 | 11.36 | 12.48 | -5.01 |
Lipid Particles | 2.89 | 4.31 | 4.14 | 4.28 | -1.39 | 5.68 | 14.56 | 18.17 | 21.42 | 0.79 | 6.33 | 18.46 | 18.47 | 21.47 | -0.01 |
Mitochondria | 0.94 | 2.24 | 1.36 | 0.46 | -2.95 | -3.22 | 6.79 | 1.89 | 4.96 | -4.60 | -2.62 | 5.61 | 1.97 | 4.67 | -4.06 |
Mitotic Spindle | -4.35 | -10.83 | -8.10 | -5.27 | 8.27 | -10.63 | -17.56 | -31.10* | -26.47* | 0.05 | -11.56 | -40.23 | -32.39 | -27.24 | 10.45 |
None | 0.38 | 2.22 | 2.28 | 1.40 | 0.39 | 5.27 | 5.97 | 6.13 | 7.64 | 1.04 | 5.21 | 6.10 | 6.31 | 5.16 | 1.00 |
Nuclear Periphery | 2.46 | 3.06 | 1.62 | -0.87 | -1.64 | 0.92 | 2.35 | 2.37 | 1.68 | 0.34 | 1.48 | 4.03 | 2.46 | 1.27 | -0.87 |
Nuclear Periphery Foci | 0.80 | 2.97 | 3.07 | 4.55 | 0.89 | 9.60 | 7.53 | 14.32 | 11.29 | 2.77 | 9.54 | 13.82 | 14.57 | 12.13 | 3.00 |
Nucleolus | 1.86 | 2.84 | 3.16 | 2.74 | 3.19 | 5.56 | 5.74 | 11.19 | 10.02 | 2.43 | 5.88 | 10.18 | 11.63 | 10.44 | 4.21 |
Nucleus | 1.71 | 2.34 | 2.08 | 0.74 | -1.50 | 4.72 | -0.06 | 7.32 | 6.24 | 2.02 | 4.97 | 5.82 | 6.82 | 3.97 | 1.64 |
Peroxisomes | 0.22 | 3.51 | 2.49 | 3.66 | -3.56 | -0.24 | 6.93 | 9.24 | 11.94 | -0.86 | -0.08 | 14.42 | 10.39 | 13.77 | -6.27 |
Vacuole | 1.94 | 1.65 | -3.06 | -4.50 | -4.31 | -1.33 | -1.89 | -5.06 | -4.44 | -0.60 | -0.87 | -1.24 | -6.65 | -6.15 | -5.50 |
Vacuole Periphery | 2.00 | 2.74 | 1.38 | -1.03 | -2.63 | -1.49 | 4.90 | -0.69 | 0.87 | -2.75 | -0.66 | 5.55 | -0.79 | -0.05 | -3.87 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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