Standard name
Description Microtubule-associated protein; component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.09 0.06 0.06 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud Site 0.06 0 0 0.06 0 0.05 0 0 0.05 0.08 0.06 0.06 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.08 0 0.09 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.13 0.23 0.33 0.19 0.15 0.09 0 0.1 0 0 0 0 0.28 0.33 0.32 0.31 0.33 0.33 0.25 0.31 0.26 0.16 0.14 0.14
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.52 0.38 0.21 0.45 0.35 0.56 0.55 0.39 0.55 0.62 0.68 0.61 0 0 0 0.14 0 0.05 0.05 0.07 0.05 0.07 0.06 0.05
Nucleus 0 0.05 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.33 0.42 0.2 0.27 0.28 0.26 0.26 0.23 0.26 0.26 0.29 0.27 0.25 0.23 0.21 0.44 0.39 0.4 0 0 0 0 0 0
Peroxisomes 0.09 0 0.28 0.2 0.15 0.14 0.15 0.2 0.05 0.07 0 0.1 0.1 0.11 0.2 0.14 0.17 0.14 0.16 0.08 0.2 0.18 0.27 0.23
SpindlePole 0.32 0.23 0.51 0.35 0.39 0.24 0.25 0.37 0.26 0.22 0.17 0.2 0.69 0.68 0.58 0.5 0.47 0.48 0.36 0.32 0.36 0.3 0.33 0.42
Vac/Vac Membrane 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 1 0 1 1 7 3 0 1 0 0 3 0 3 0 1 0 1 0 2 4 11 10
Bud 1 1 4 1 9 10 45 36 40 35 22 31 1 0 1 1 2 1 2 1 1 9 16 11
Bud Neck 0 0 3 3 3 4 6 9 9 4 3 4 2 1 1 2 1 0 1 1 1 10 8 7
Bud Site 11 0 12 14 14 21 23 21 31 52 33 41 3 3 3 4 3 2
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 2 0 0 1 1 0 0 0 0 0 0 0 0
Cytoplasm 5 0 4 6 15 12 19 11 8 13 6 5 11 22 27 11 26 20 0 0 0 1 2 2
Endoplasmic Reticulum 1 0 0 0 0 1 0 0 0 0 0 0 0 1 0 8 7 4 0 0 1 0 0 0
Endosome 23 9 108 46 51 34 16 65 21 13 9 19 64 111 107 111 96 71 45 23 96 32 52 61
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 2 3 0 4 1 4 1
Mitochondria 89 15 68 111 119 214 291 243 353 427 372 419 1 0 4 48 12 10 8 5 18 14 21 23
Nucleus 4 2 3 3 11 13 9 13 13 14 31 17 5 4 6 6 7 6 0 0 0 2 1 3
Nuclear Periphery 2 0 0 1 2 4 9 16 16 11 15 17 0 0 1 4 3 3 0 0 0 1 1 0
Nucleolus 56 17 65 67 94 98 138 145 169 179 159 186 57 79 71 154 112 87 5 3 6 9 10 6
Peroxisomes 16 0 91 49 52 53 80 126 31 46 21 68 23 38 66 51 48 30 30 6 71 37 101 103
SpindlePole 55 9 168 86 132 94 133 228 169 154 94 134 157 231 192 178 135 103 67 24 130 60 125 183
Vac/Vac Membrane 2 3 9 4 6 8 2 4 9 6 8 7 7 5 13 16 12 16 0 1 0 2 5 3
Unique Cell Count 172 40 329 248 337 384 529 623 638 691 551 684 226 338 332 354 290 216 187 76 365 202 378 442
Labelled Cell Count 266 56 536 391 509 567 778 920 869 956 773 950 334 495 497 596 466 355 187 76 365 202 378 442


Microtubule, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 6.3 5.4 5.0 5.3 4.5 4.5 4.6 4.3 4.3 4.0 4.2 7.3 7.2 7.0 8.3 8.3 8.7 6.9 6.9 7.0
Std Deviation (1e-4) 0.7 1.1 0.8 1.0 1.5 0.9 1.4 1.0 0.9 1.1 0.9 1.0 1.2 1.3 1.2 1.7 1.7 1.7 1.2 1.2 1.1
Intensity Change (Log2) -0.11 -0.03 -0.25 -0.25 -0.24 -0.32 -0.34 -0.43 -0.35 0.44 0.42 0.36 0.61 0.62 0.69 0.36 0.34 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1.4 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1.1 0.3 0 0 0 0 0 0 0 -1.7 -2.4 -2.4 -2.2 -2.1 -2.0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.5 1.7 2.1 0.6 0 0.8 0 0 2.6 3.5 4.2 1.7 4.5 4.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -3.8 -5.4 0 0 0 0 0 0 0 -1.1 0 -0.2 -0.4 0.1 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 6.2 4.2 0 0 0 0 0 0 0 -7.1 -8.8 -8.0 -2.5 -6.1 -5.2
Nucleus 0 2.1 2.2 0 1.3 1.3 1.3 3.5 1.7 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.1 2.5 0 0 0 0 0 0 0 1.5 1.1 0.5 6.6 5.2 5.2
Peroxisomes -2.2 -3.8 0 0 0 0 0 0 0 -5.0 -5.4 -2.4 -4.3 -3.3 -3.8
SpindlePole -3.9 -3.1 0 0 0 0 0 0 0 4.3 4.6 1.7 -0.2 -1.1 -0.8
Vacuole -0.9 -0.8 -0.6 0 0 -1.4 0 -1.3 -2.0 0.3 -1.1 0.8 1.2 1.0 2.5


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bik1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available