Standard name
Human Ortholog
Description Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Cytoplasm 0.96 0.95 0.98 0.96 0.99 0.91 0.93 – 0.85 0.87 0.77 0.81 0.99 1.0 0.99 – 0.94 – 0.84 0.85 0.79 0.88 0.81 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0.07 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Mitochondria 0 0.13 0 0 0 0.12 0.06 – 0.2 0.18 0.34 0.25 0 0 0 – 0 – 0.06 0.06 0.09 0 0.06 0.08
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 1 0 0 0 1 0 0 – 0 – 1 1 1 1 6 4
Bud 0 2 0 3 0 1 3 – 5 5 6 5 0 0 0 – 0 – 1 0 1 2 12 7
Bud Neck 1 0 2 4 5 7 17 – 12 15 9 8 1 0 0 – 2 – 0 0 0 0 1 4
Bud Site 0 0 0 0 0 0 0 – 0 0 4 3 1 0 0 – 0 – – – – – – –
Cell Periphery 2 0 3 2 1 0 1 – 1 1 0 2 1 2 1 – 5 – 1 0 0 0 2 2
Cytoplasm 175 107 56 184 263 187 465 – 256 386 212 341 352 436 464 – 207 – 158 98 69 198 383 275
Endoplasmic Reticulum 1 1 0 0 0 3 0 – 0 1 0 0 0 1 0 – 15 – 0 0 1 1 4 0
Endosome 1 0 0 0 0 0 3 – 1 1 0 0 0 0 1 – 2 – 2 1 0 0 5 6
Golgi 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 – 3 – 0 2 0 0 1 0
Mitochondria 8 15 1 8 0 24 30 – 61 79 93 106 1 1 1 – 3 – 11 6 8 9 29 29
Nucleus 0 0 1 0 2 5 2 – 2 2 3 4 0 1 1 – 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 – 0 0 0 3 0 0 0 – 0 – 0 0 0 0 0 3
Nucleolus 0 1 0 0 0 0 0 – 0 0 0 1 0 0 0 – 1 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 2 – 0 0 0 0 1 0
SpindlePole 0 0 0 0 1 2 22 – 4 15 4 12 0 0 0 – 0 – 2 0 0 3 11 4
Vac/Vac Membrane 3 1 0 2 1 0 2 – 0 2 0 5 0 0 5 – 5 – 1 1 0 1 2 3
Unique Cell Count 183 113 57 191 266 205 498 300 442 277 421 354 438 468 220 189 116 89 225 474 353
Labelled Cell Count 191 128 63 203 273 229 546 343 507 331 490 357 441 473 245 189 116 89 225 474 353


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.8 4.5 4.6 5.1 3.3 4.0 – 3.5 3.6 3.5 3.3 4.6 4.9 5.2 4.9 5.1 4.8 4.3 4.2 4.5
Std Deviation (1e-4) 0.8 0.9 0.7 2.0 1.5 1.0 1.2 – 1.4 1.1 1.3 1.0 1.6 1.8 2.0 1.2 1.2 0.8 2.0 1.7 2.0
Intensity Change (Log2) – – – 0.03 0.18 -0.46 -0.18 – -0.35 -0.34 -0.38 -0.42 0.05 0.12 0.22 0.12 0.19 0.09 -0.08 -0.09 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2829 2.7372 2.2573 0.5807 1.6815 1.9968 1.6469 2.9782 2.971 3.4317 0.6657 3.5947 3.2054 4.8888 4.3373 2.0888 4.2855 5.6937
Actin 0.0167 0.0012 0.0031 0.0609 0.0006 0.0018 0.0158 0.0003 0.0062 0.0051 0.0212 0.0239 0.0498 0.001 0.0094 0.0064 0.0221 0.0044
Bud 0.0015 0.0042 0.0057 0.0009 0.0043 0.0005 0.0012 0.0035 0.002 0.0003 0.0043 0.0005 0.0014 0.0032 0.0009 0.0008 0.0004 0.0015
Bud Neck 0.0026 0.0003 0.0007 0.0007 0.0004 0.0036 0.0008 0.0003 0.0015 0.0008 0.0011 0.0023 0.0025 0.0003 0.0006 0.0018 0.0008 0.0023
Bud Periphery 0.001 0.0028 0.0023 0.0009 0.0027 0.0004 0.0009 0.001 0.0023 0.0002 0.0048 0.0006 0.0013 0.0011 0.0009 0.0009 0.0004 0.0021
Bud Site 0.0023 0.0142 0.0036 0.0018 0.0001 0.0002 0.0055 0.0079 0.0054 0.0005 0.0014 0.0004 0.0067 0.0028 0.0011 0.002 0.0009 0.0003
Cell Periphery 0.0002 0.0003 0.0003 0.0001 0.0001 0.0001 0.0002 0.0002 0.0004 0.0001 0.0002 0.0001 0.0004 0.0002 0.0002 0.0004 0.0001 0.0001
Cytoplasm 0.474 0.631 0.6065 0.2277 0.5033 0.5923 0.5325 0.6884 0.6631 0.5887 0.3413 0.6276 0.4702 0.6532 0.6295 0.4998 0.6727 0.7128
Cytoplasmic Foci 0.0133 0.0083 0.0225 0.0151 0.0248 0.0127 0.0232 0.004 0.0125 0.0083 0.0302 0.0202 0.0255 0.0057 0.0044 0.0465 0.0034 0.0114
Eisosomes 0.0002 0.0001 0.0001 0.0003 0 0.0001 0.0002 0.0001 0.0003 0.0002 0.0002 0.0003 0.0003 0 0.0001 0.0004 0.0005 0
Endoplasmic Reticulum 0.005 0.0049 0.0054 0.0034 0.0036 0.0019 0.0062 0.0016 0.0036 0.0023 0.0095 0.0038 0.0081 0.0019 0.0028 0.0056 0.002 0.0044
Endosome 0.0119 0.0022 0.003 0.0211 0.0036 0.0032 0.0115 0.001 0.0051 0.0083 0.0278 0.0129 0.0323 0.0013 0.0021 0.0205 0.0016 0.0046
Golgi 0.0021 0.0002 0.001 0.0126 0.0002 0.0004 0.0034 0.0001 0.0025 0.0064 0.0068 0.0033 0.0115 0.0001 0.0002 0.0115 0.0019 0.0004
Lipid Particles 0.0028 0.0003 0.006 0.0079 0.0006 0.0019 0.0084 0.0002 0.0021 0.013 0.0198 0.0039 0.0106 0.0001 0.0002 0.0552 0.0012 0.0006
Mitochondria 0.0012 0.0005 0.0006 0.0042 0.0004 0.0003 0.003 0.0002 0.0017 0.0027 0.004 0.0019 0.0078 0.0003 0.0004 0.0049 0.0263 0.0011
None 0.4088 0.2891 0.2919 0.5924 0.3486 0.3086 0.3402 0.2686 0.2467 0.3131 0.4586 0.2334 0.331 0.2833 0.3155 0.3001 0.2326 0.1852
Nuclear Periphery 0.0086 0.003 0.0036 0.0056 0.0159 0.0059 0.0082 0.0017 0.0039 0.0028 0.0137 0.0107 0.0104 0.0025 0.0025 0.001 0.0014 0.0066
Nucleolus 0.0028 0.0003 0.0002 0.0002 0.0004 0.0005 0.0003 0.0001 0.0007 0.0021 0.0025 0.0003 0.0005 0.0001 0.0001 0.0003 0.0001 0.0003
Nucleus 0.0296 0.0328 0.0238 0.0286 0.0791 0.0573 0.0197 0.0183 0.0269 0.0222 0.0087 0.0404 0.0123 0.0395 0.0252 0.0042 0.0228 0.054
Peroxisomes 0.0017 0.0009 0.0142 0.0104 0.0005 0.0006 0.0087 0.0001 0.0014 0.0028 0.0197 0.0028 0.0066 0.0001 0.0002 0.0335 0.0075 0.0006
Punctate Nuclear 0.0102 0.002 0.0041 0.0034 0.0034 0.0056 0.0075 0.0013 0.0074 0.0189 0.0206 0.0088 0.0075 0.0021 0.0024 0.002 0.0008 0.0055
Vacuole 0.0029 0.0016 0.0014 0.0014 0.0061 0.0016 0.002 0.001 0.0037 0.0008 0.0024 0.0014 0.0023 0.001 0.0011 0.0015 0.0004 0.0014
Vacuole Periphery 0.0006 0.0002 0.0002 0.0006 0.0011 0.0004 0.0007 0.0001 0.0005 0.0003 0.0012 0.0007 0.0011 0.0001 0.0002 0.0005 0.0002 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.4274 3.3896 3.7729 1.1071 1.229 1.8948 10.7101 7.5177 5.4335 2.7215
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1782 1536 147 1581 1235 1900 1985 1530 3017 3436 2132 3111

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 709.47 738.69 960.73 783.69 686.86 757.07 805.76 840.71 700.21 748.85 816.45 811.73
Standard Deviation 104.66 116.61 158.46 239.84 87.19 265.22 106.82 124.44 98.52 212.27 117.88 194.07
Intensity Change Log 2 — 0.058227 0.437389 0.143541 — 0.140411 0.230334 0.291592 — 0.099239 0.339246 0.218267

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000137 0.000366 0.000972 0.000171 0.000072 0.000351 0.000111 0.000822 0.000110 0.000357 0.000170 0.000491
Bud Neck 0.015525 0.026228 0.002655 0.006757 0.011347 0.037177 0.003402 0.006327 0.013815 0.032282 0.003351 0.006546
Bud Site 0.005310 0.016883 0.004307 0.015769 0.004313 0.019489 0.003780 0.027930 0.004902 0.018324 0.003817 0.021750
Cell Periphery 0.000215 0.000147 0.000362 0.000182 0.000152 0.000136 0.000086 0.000172 0.000189 0.000141 0.000105 0.000177
Cytoplasm 0.501830* 0.470359* 0.276273* 0.480609* 0.545671* 0.556045* 0.534946* 0.531585* 0.519776* 0.517741* 0.517111* 0.505679*
Cytoplasmic Foci 0.231702* 0.225759* 0.016977 0.023013 0.198078* 0.129945 0.009911 0.025405 0.217938* 0.172777 0.010398 0.024189
Eisosomes 0.000203 0.000101 0.000098 0.000041 0.000121 0.000095 0.000020 0.000039 0.000169 0.000098 0.000026 0.000040
Endoplasmic Reticulum 0.001065 0.001212 0.009909 0.001399 0.000765 0.000379 0.001449 0.001399 0.000942 0.000752 0.002032 0.001399
Endosome 0.009278 0.018792 0.004113 0.005349 0.008606 0.006561 0.000952 0.007224 0.009003 0.012029 0.001170 0.006271
Golgi 0.002723 0.006043 0.000209 0.000791 0.002584 0.003208 0.000048 0.006257 0.002666 0.004475 0.000059 0.003479
Lipid Particles 0.010418 0.006075 0.003510 0.001282 0.007129 0.003081 0.000570 0.001200 0.009072 0.004419 0.000772 0.001242
Mitochondria 0.007087 0.010285 0.000453 0.005126 0.003140 0.007053 0.000302 0.003267 0.005472 0.008498 0.000313 0.004212
Mitotic Spindle 0.002616 0.003651 0.000554 0.012665 0.005106 0.006940 0.001762 0.041760 0.003635 0.005470 0.001678 0.026974
None 0.004018 0.004853 0.003259 0.004411 0.005637 0.007075 0.008292 0.005895 0.004680 0.006082 0.007945 0.005141
Nuclear Periphery 0.000471 0.000601 0.001816 0.001930 0.000417 0.000267 0.000993 0.001588 0.000449 0.000417 0.001050 0.001762
Nuclear Periphery Foci 0.000749 0.000939 0.004616 0.000613 0.000493 0.000295 0.000856 0.001494 0.000644 0.000583 0.001115 0.001046
Nucleolus 0.001090 0.002430 0.000893 0.000752 0.001732 0.001565 0.000373 0.000673 0.001353 0.001952 0.000408 0.000713
Nucleus 0.161020* 0.128731 0.566778* 0.347759* 0.166632* 0.168872* 0.397217* 0.246491* 0.163317* 0.150927* 0.408908* 0.297955*
Peroxisomes 0.007014 0.011450 0.000454 0.000907 0.003142 0.008570 0.000100 0.001217 0.005429 0.009858 0.000125 0.001059
Vacuole 0.036584 0.062317 0.101534 0.087564 0.034169 0.042042 0.034750 0.086390 0.035596 0.051106 0.039355 0.086987
Vacuole Periphery 0.000946 0.002778 0.000258 0.002908 0.000693 0.000855 0.000081 0.002865 0.000843 0.001714 0.000093 0.002887

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.41 -8.95 -2.25 5.02 8.60 -3.06 -5.88 -4.05 -1.17 -3.28 -4.65 -6.96 -4.29 0.12 -1.93
Bud Neck -5.01 10.84 7.59 9.90 -3.67 -10.53 5.29 2.41 13.47 -4.68 -11.32 10.33 7.25 16.74 -4.82
Bud Site -8.55 -0.50 -2.72 3.95 -2.41 -8.48 0.72 -6.24 0.46 -6.67 -11.75 1.53 -6.63 2.77 -7.11
Cell Periphery 4.49 -4.79 4.07 1.32 7.06 1.25 5.48 1.86 0.97 -2.88 4.62 7.54 4.68 1.57 -1.06
Cytoplasm 3.57 14.65 11.83 7.50 -8.59 -1.06 6.70 11.48 13.29 5.67 0.53 7.30 15.99 15.29 6.95
Cytoplasmic Foci 0.71 40.09 41.64 36.66 -1.44 9.86 33.19 30.97 26.77 -7.65 8.81 54.60 51.73 44.00 -8.47
Eisosomes 8.32 5.81 13.60 12.55 7.02 3.11 17.96 13.14 6.30 -11.48 8.68 19.07 16.91 11.33 -8.81
Endoplasmic Reticulum -0.79 -10.39 -6.89 -5.61 9.42 3.21 -8.90 -10.32 -17.74 -0.73 1.59 -11.48 -11.33 -15.11 4.19
Endosome -6.95 5.56 6.41 12.35 1.91 2.76 13.13 5.19 2.83 -6.12 -3.90 14.99 8.03 11.66 -6.42
Golgi -4.32 9.68 8.05 7.79 -1.18 -0.72 4.51 0.22 0.81 -2.99 -3.28 9.43 2.72 5.08 -3.35
Lipid Particles 5.22 8.66 13.06 11.14 6.88 5.27 9.82 8.69 4.02 -9.05 8.07 16.65 15.65 10.22 -7.28
Mitochondria -2.00 6.45 3.38 5.35 -2.79 -3.32 4.02 1.66 5.08 -3.08 -2.98 7.67 3.97 7.19 -4.10
Mitotic Spindle -1.05 2.20 -5.63 -5.30 -6.84 -0.77 2.14 -7.60 -7.28 -9.27 -1.56 2.03 -9.64 -8.75 -11.05
None -1.26 2.36 -0.74 0.73 -2.95 -1.36 -1.14 -0.07 1.66 1.41 -2.37 -2.68 -0.76 1.97 2.34
Nuclear Periphery -0.65 -12.23 -16.43 -10.99 -5.37 2.75 -14.81 -17.40 -19.39 -10.64 0.36 -18.38 -23.45 -19.49 -14.63
Nuclear Periphery Foci -0.74 -7.69 0.57 1.54 8.21 2.10 -5.13 -3.53 -3.98 -2.21 0.56 -5.02 -2.91 -3.32 -0.08
Nucleolus -3.73 2.29 2.25 4.71 -0.34 0.47 2.49 1.33 2.30 -4.47 -1.94 3.82 1.92 5.30 -4.77
Nucleus 5.51 -16.88 -28.90 -32.57 6.14 -0.46 -33.59 -16.60 -16.40 14.37 2.65 -40.55 -32.18 -33.90 11.44
Peroxisomes -4.04 8.74 8.51 12.85 -0.69 -5.01 7.15 5.27 8.09 -3.66 -5.48 11.11 9.83 14.13 -4.50
Vacuole -9.16 -8.38 -21.41 -14.65 1.80 -3.39 -8.17 -22.71 -21.16 -17.44 -8.71 -10.70 -31.12 -25.84 -19.21
Vacuole Periphery -4.65 5.60 -0.70 2.23 -2.04 -1.24 8.39 -1.42 -0.93 -3.34 -4.48 9.60 -1.26 1.26 -3.73
Standard name
Human Ortholog
Description Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin
Localization
Cell Percentages cytoplasm (38%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Frm2

Frm2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Frm2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available