Standard name
Description Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.95 0.98 0.96 0.99 0.91 0.93 0.85 0.87 0.77 0.81 0.99 1.0 0.99 0.94 0.84 0.85 0.79 0.88 0.81 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.13 0 0 0 0.12 0.06 0.2 0.18 0.34 0.25 0 0 0 0 0.06 0.06 0.09 0 0.06 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 1 1 1 1 6 4
Bud 0 2 0 3 0 1 3 5 5 6 5 0 0 0 0 1 0 1 2 12 7
Bud Neck 1 0 2 4 5 7 17 12 15 9 8 1 0 0 2 0 0 0 0 1 4
Bud Site 0 0 0 0 0 0 0 0 0 4 3 1 0 0 0
Cell Periphery 2 0 3 2 1 0 1 1 1 0 2 1 2 1 5 1 0 0 0 2 2
Cytoplasm 175 107 56 184 263 187 465 256 386 212 341 352 436 464 207 158 98 69 198 383 275
Endoplasmic Reticulum 1 1 0 0 0 3 0 0 1 0 0 0 1 0 15 0 0 1 1 4 0
Endosome 1 0 0 0 0 0 3 1 1 0 0 0 0 1 2 2 1 0 0 5 6
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 3 0 2 0 0 1 0
Mitochondria 8 15 1 8 0 24 30 61 79 93 106 1 1 1 3 11 6 8 9 29 29
Nucleus 0 0 1 0 2 5 2 2 2 3 4 0 1 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 3 0 0 0 0 0 0 0 0 0 3
Nucleolus 0 1 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1 0
SpindlePole 0 0 0 0 1 2 22 4 15 4 12 0 0 0 0 2 0 0 3 11 4
Vac/Vac Membrane 3 1 0 2 1 0 2 0 2 0 5 0 0 5 5 1 1 0 1 2 3
Unique Cell Count 183 113 57 191 266 205 498 300 442 277 421 354 438 468 220 189 116 89 225 474 353
Labelled Cell Count 191 128 63 203 273 229 546 343 507 331 490 357 441 473 245 189 116 89 225 474 353


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.8 4.5 4.6 5.1 3.3 4.0 3.5 3.6 3.5 3.3 4.6 4.9 5.2 4.9 5.1 4.8 4.3 4.2 4.5
Std Deviation (1e-4) 0.8 0.9 0.7 2.0 1.5 1.0 1.2 1.4 1.1 1.3 1.0 1.6 1.8 2.0 1.2 1.2 0.8 2.0 1.7 2.0
Intensity Change (Log2) 0.03 0.18 -0.46 -0.18 -0.35 -0.34 -0.38 -0.42 0.05 0.12 0.22 0.12 0.19 0.09 -0.08 -0.09 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Frm2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available