Standard name
Description Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.06 0.05 0.06 0.08 0.07 0 0 0
Bud 0 0 0.07 0.05 0.06 0 0 0 0 0.05 0.05 0 0 0.05 0.06 0.08 0.05 0 0.06 0.05 0 0 0 0
Bud Neck 0 0 0.06 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0.09 0.12 0.06 0.14 0.12 0.11 0.12 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.81 0.78 0.83 0.87 0.86 0.85 0.82 0.8 0.73 0.75 0.66 0.68 0.87 0.9 0.93 0.77 0.83 0.83 0.54 0.56 0.51 0.66 0.92 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.3 0.13 0.11 0.07 0.17 0.24 0.26 0.4 0.38 0.42 0.49 0 0 0 0 0 0 0.06 0.09 0.13 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0 0 0.05 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 1 3 5 0 4 4 0 2 3 2 0 5 5 0 17 7 13 8 6 8 2 2 12
Bud 3 1 7 9 16 14 20 19 10 18 15 15 8 18 27 12 6 9 7 4 4 0 2 9
Bud Neck 2 0 6 10 14 14 19 22 8 7 6 8 13 12 1 13 15 15 19 9 12 10 1 4
Bud Site 2 0 4 6 3 5 3 11 3 2 4 6 6 1 4 2 3 3
Cell Periphery 2 0 1 0 2 2 1 2 3 2 1 6 1 1 1 5 4 3 0 0 2 0 1 2
Cytoplasm 93 36 83 171 239 310 390 505 217 269 180 297 272 317 415 111 105 208 75 45 59 55 343 354
Endoplasmic Reticulum 2 1 1 1 0 0 2 0 0 1 2 0 1 0 0 7 3 19 1 0 0 0 0 4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 3 7 4 1 1 3 5 1 2 2 0 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 1
Mitochondria 15 14 13 21 20 61 113 167 117 136 116 217 9 0 20 4 2 4 8 7 15 3 4 11
Nucleus 1 0 0 0 1 0 1 7 0 0 1 1 1 1 0 1 1 1 0 0 0 0 1 0
Nuclear Periphery 1 0 0 1 0 0 2 1 1 1 1 0 1 1 0 0 0 0 0 1 0 0 0 1
Nucleolus 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 2 0 0 0 0 1 0 0 3 3 2 0 0 1 1 0 0 0 0 0
SpindlePole 1 0 1 2 9 7 9 16 6 2 12 15 10 2 1 7 7 16 3 2 5 1 1 6
Vac/Vac Membrane 0 1 0 0 0 0 1 0 1 0 0 2 0 1 0 1 0 1 2 2 2 2 3 3
Unique Cell Count 115 46 100 196 277 363 477 634 296 359 273 439 311 352 447 144 127 251 139 82 117 84 372 424
Labelled Cell Count 127 54 119 226 307 417 565 750 368 442 340 567 334 369 475 181 154 297 139 82 117 84 372 424


Ambiguous, Bud, Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 3.4 2.9 2.8 3.1 2.8 2.6 2.7 2.3 2.4 2.4 2.1 4.3 4.3 3.7 5.2 5.2 5.9 4.0 3.9 4.5
Std Deviation (1e-4) 0.6 0.8 0.4 0.6 1.0 0.7 0.9 0.6 0.6 1.1 1.0 0.7 0.9 0.7 0.7 0.9 1.0 1.8 1.4 0.9 1.5
Intensity Change (Log2) -0.02 0.13 -0.02 -0.12 -0.08 -0.31 -0.28 -0.25 -0.42 0.57 0.57 0.38 0.85 0.86 1.03 0.5 0.44 0.64


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kcc4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available