Standard name
Description Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.06 0.05 0.06 0.08 0.07 0 0 0
Bud 0 0 0.07 0.05 0.06 0 0 0 0 0.05 0.05 0 0 0.05 0.06 0.08 0.05 0 0.06 0.05 0 0 0 0
Bud Neck 0 0 0.06 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0.09 0.12 0.06 0.14 0.12 0.11 0.12 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.81 0.78 0.83 0.87 0.86 0.85 0.82 0.8 0.73 0.75 0.66 0.68 0.87 0.9 0.93 0.77 0.83 0.83 0.54 0.56 0.51 0.66 0.92 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.3 0.13 0.11 0.07 0.17 0.24 0.26 0.4 0.38 0.42 0.49 0 0 0 0 0 0 0.06 0.09 0.13 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0 0 0.05 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 1 3 5 0 4 4 0 2 3 2 0 5 5 0 17 7 13 8 6 8 2 2 12
Bud 3 1 7 9 16 14 20 19 10 18 15 15 8 18 27 12 6 9 7 4 4 0 2 9
Bud Neck 2 0 6 10 14 14 19 22 8 7 6 8 13 12 1 13 15 15 19 9 12 10 1 4
Bud Site 2 0 4 6 3 5 3 11 3 2 4 6 6 1 4 2 3 3 – – – – – –
Cell Periphery 2 0 1 0 2 2 1 2 3 2 1 6 1 1 1 5 4 3 0 0 2 0 1 2
Cytoplasm 93 36 83 171 239 310 390 505 217 269 180 297 272 317 415 111 105 208 75 45 59 55 343 354
Endoplasmic Reticulum 2 1 1 1 0 0 2 0 0 1 2 0 1 0 0 7 3 19 1 0 0 0 0 4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 3 7 4 1 1 3 5 1 2 2 0 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 1
Mitochondria 15 14 13 21 20 61 113 167 117 136 116 217 9 0 20 4 2 4 8 7 15 3 4 11
Nucleus 1 0 0 0 1 0 1 7 0 0 1 1 1 1 0 1 1 1 0 0 0 0 1 0
Nuclear Periphery 1 0 0 1 0 0 2 1 1 1 1 0 1 1 0 0 0 0 0 1 0 0 0 1
Nucleolus 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 2 0 0 0 0 1 0 0 3 3 2 0 0 1 1 0 0 0 0 0
SpindlePole 1 0 1 2 9 7 9 16 6 2 12 15 10 2 1 7 7 16 3 2 5 1 1 6
Vac/Vac Membrane 0 1 0 0 0 0 1 0 1 0 0 2 0 1 0 1 0 1 2 2 2 2 3 3
Unique Cell Count 115 46 100 196 277 363 477 634 296 359 273 439 311 352 447 144 127 251 139 82 117 84 372 424
Labelled Cell Count 127 54 119 226 307 417 565 750 368 442 340 567 334 369 475 181 154 297 139 82 117 84 372 424


Ambiguous, Bud, Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 3.4 2.9 2.8 3.1 2.8 2.6 2.7 2.3 2.4 2.4 2.1 4.3 4.3 3.7 5.2 5.2 5.9 4.0 3.9 4.5
Std Deviation (1e-4) 0.6 0.8 0.4 0.6 1.0 0.7 0.9 0.6 0.6 1.1 1.0 0.7 0.9 0.7 0.7 0.9 1.0 1.8 1.4 0.9 1.5
Intensity Change (Log2) – – – -0.02 0.13 -0.02 -0.12 -0.08 -0.31 -0.28 -0.25 -0.42 0.57 0.57 0.38 0.85 0.86 1.03 0.5 0.44 0.64


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2511 -0.3931 -0.3621 -0.8635 1.8822 -0.713 1.1115 0.9818 1.4111 0.9589 0.7021 0.6688 -1.7338 -0.9157 -0.8131 -1.0187 -1.0179 -0.9329
Actin 0.1659 0.0067 0.0265 0.0042 0.1289 0.0041 0.1075 0.0011 0.0367 0.002 0.0323 0.0143 0.0007 0.0006 0.0149 0.0022 0.0083 0.0075
Bud 0.0026 0.008 0.0094 0.0003 0.0161 0.0003 0.0042 0.001 0.0014 0.0008 0.0005 0.0003 0.0007 0.0052 0.0006 0.0004 0.0002 0.0001
Bud Neck 0.0188 0.0177 0.3706 0.4938 0.0088 0.1569 0.0234 0.0121 0.3487 0.27 0.0916 0.0615 0.0134 0.0209 0.2877 0.2509 0.0594 0.0739
Bud Periphery 0.0042 0.0043 0.007 0.0004 0.0287 0.0005 0.0095 0.0008 0.0017 0.0011 0.001 0.0004 0.0013 0.0031 0.0012 0.0005 0.0004 0.0002
Bud Site 0.0444 0.1046 0.1343 0.0016 0.0187 0.0011 0.0808 0.2129 0.0998 0.01 0.002 0.0008 0.0727 0.1433 0.0707 0.0006 0.0007 0.0014
Cell Periphery 0.0024 0.0028 0.0028 0.0005 0.0048 0.0003 0.0018 0.0018 0.0014 0.0008 0.0006 0.0002 0.0015 0.0021 0.0029 0.0005 0.0001 0.0003
Cytoplasm 0.0723 0.1334 0.0584 0.139 0.263 0.1732 0.1543 0.2119 0.1494 0.2976 0.2232 0.3661 0.0465 0.0363 0.0477 0.0344 0.0554 0.0521
Cytoplasmic Foci 0.0352 0.0266 0.041 0.0299 0.007 0.0174 0.0511 0.0177 0.024 0.022 0.0269 0.0102 0.0147 0.0076 0.0204 0.01 0.0097 0.0116
Eisosomes 0.003 0.0012 0.0015 0.0003 0.0011 0.0002 0.001 0.0002 0.0006 0.0003 0.0006 0.0001 0.0006 0.0009 0.0026 0.0008 0.0003 0.0004
Endoplasmic Reticulum 0.0094 0.0015 0.0013 0.0009 0.0146 0.0013 0.0032 0.0007 0.0028 0.0006 0.0014 0.0023 0.0015 0.0005 0.0005 0.0004 0.0005 0.0004
Endosome 0.0414 0.0028 0.0045 0.0128 0.0654 0.0058 0.0267 0.011 0.0145 0.0019 0.0143 0.0072 0.0048 0.0003 0.0009 0.0003 0.0071 0.0068
Golgi 0.0191 0.0007 0.0029 0.0016 0.0137 0.0025 0.0177 0.001 0.0103 0.0003 0.0311 0.0059 0.0003 0.0001 0.0015 0.0001 0.0052 0.0061
Lipid Particles 0.0116 0.0018 0.0059 0.017 0.0028 0.0031 0.0253 0.001 0.0104 0.0001 0.0157 0.0017 0.0048 0.0004 0.0025 0.0005 0.0244 0.0029
Mitochondria 0.0319 0.0044 0.002 0.0548 0.017 0.003 0.0224 0.0002 0.0048 0.0004 0.0488 0.0039 0.0004 0.0005 0.0021 0.0003 0.0072 0.0013
None 0.4836 0.672 0.3107 0.2343 0.2316 0.6242 0.4085 0.5182 0.2689 0.387 0.4947 0.5205 0.8301 0.7732 0.53 0.6809 0.7817 0.8288
Nuclear Periphery 0.0193 0.0003 0.0012 0.0005 0.0775 0.0008 0.0144 0.0004 0.0058 0.0002 0.0006 0.0012 0.001 0.0002 0.0002 0.0007 0.0014 0.0011
Nucleolus 0.0019 0.0038 0.002 0.0005 0.0039 0.0003 0.0012 0.0002 0.0008 0.0003 0.0003 0.0001 0.0007 0.0011 0.0016 0.0018 0.0002 0.0002
Nucleus 0.0053 0.0022 0.002 0.0011 0.0586 0.0013 0.0035 0.001 0.0033 0.0014 0.0006 0.0009 0.0009 0.0018 0.0014 0.003 0.0003 0.0003
Peroxisomes 0.0088 0.0018 0.0069 0.0019 0.0013 0.0003 0.0225 0.0011 0.0092 0.0002 0.0095 0.0011 0.0004 0.0004 0.0074 0.0003 0.0344 0.0011
Punctate Nuclear 0.0099 0.0017 0.0078 0.0009 0.0022 0.0011 0.0069 0.0039 0.0041 0.0007 0.0013 0.0009 0.0018 0.0012 0.0026 0.0108 0.0021 0.0029
Vacuole 0.0055 0.0016 0.0012 0.0021 0.0206 0.0014 0.0108 0.0015 0.0008 0.0022 0.0018 0.0004 0.001 0.0005 0.0005 0.0005 0.0004 0.0002
Vacuole Periphery 0.0034 0.0001 0.0002 0.0016 0.0138 0.001 0.0035 0.0002 0.0006 0.0001 0.0012 0.0002 0.0001 0 0.0001 0.0001 0.0004 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.0082 2.2173 5.6411 13.9592 13.5527 23.0191 5.6848 4.8636 10.6064 20.8917
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –