Standard name
Human Ortholog
Description Subunit of a two-component GTPase-activating protein, Bfa1p-Bub2p; contributes to GAP activity, inactivating Tem1 by stimulating GTP hydrolysis following damage or misalignment of the mitotic spindle; functions as a guanine-nucleotide exchange inhibitor (GDI) for Tem1p; involved in multiple cell cycle checkpoint pathways that control mitotic exit; required when telomeres are damaged, but not for all types of chromosomal DNA damage; phosphorylated by the Polo-like kinase Cdc5p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.05 0.05 0.07 0.07 0.07 0.12 0.13 0.1 0 0 0 0 0 0 0 0 0 0.07 0 0
Bud Neck 0 0 0.09 0 0 0.06 0 0 0.09 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0.07 0 0 0 0 0.05 0 0.08 0.09 0.09 0.12 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.15 0.11 0.17 0.18 0.14 0.12 0.11 0.12 0.14 0.13 0.08 0.29 0.28 0.29 0.09 0.11 0.09 0.06 0.05 0.06 0.44 0.5 0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.09 0.17 0 0 0 0 0 0 0 0 0 0.06 0.06 0.12 0.05 0.07 0.09 0 0.06 0 0.05 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0.06 0.05 0.13 0.1 0.18 0.15 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.06 0.08 0.08 0 0 0 0 0 0
Peroxisomes 0.07 0.15 0 0 0 0 0 0 0 0.05 0 0 0 0.06 0.1 0.13 0.12 0 0 0 0 0 0
SpindlePole 0.77 0.76 0.78 0.76 0.83 0.75 0.84 0.75 0.77 0.71 0.81 0.64 0.67 0.62 0.8 0.82 0.81 0.83 0.78 0.76 0.33 0.35 0.26
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 2 4 4 5 3 1 3 3 2 1 1 0 1 0 1 2 1 1 0 1 4
Bud 16 8 4 8 7 16 25 15 27 31 23 1 2 2 3 2 3 10 9 4 5 6 7
Bud Neck 10 6 11 6 6 12 15 7 19 12 11 5 2 4 2 4 2 3 8 0 3 3 2
Bud Site 30 3 1 2 1 11 15 17 19 21 28 0 0 1 4 5 1
Cell Periphery 2 1 0 0 0 0 1 0 0 0 0 1 0 1 3 0 0 0 0 0 0 0 0
Cytoplasm 63 33 22 30 22 25 36 25 30 32 20 40 62 72 27 35 20 25 16 9 36 85 116
Endoplasmic Reticulum 0 1 0 0 1 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0
Endosome 36 51 4 1 2 7 6 4 2 2 3 8 14 31 16 23 20 16 18 6 4 5 3
Golgi 0 1 0 1 0 0 0 0 0 0 0 0 0 0 2 2 1 2 1 1 0 0 0
Mitochondria 19 10 1 6 2 13 16 27 21 43 35 0 0 0 6 7 4 4 5 7 0 1 4
Nucleus 1 0 0 1 2 3 3 6 1 2 3 0 0 3 6 2 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 10 13 2 0 1 8 12 8 9 12 9 0 0 4 17 26 18 3 0 0 0 0 0
Peroxisomes 28 46 1 2 0 4 4 9 1 11 4 4 4 15 30 44 25 2 5 1 0 0 0
SpindlePole 325 235 100 129 128 163 280 160 170 173 191 88 146 155 236 271 173 365 245 115 27 60 50
Vac/Vac Membrane 5 0 1 0 0 3 1 0 0 0 0 0 1 1 2 5 2 1 0 0 0 1 0
Unique Cell Count 422 308 128 169 155 216 334 212 221 243 236 137 218 249 294 331 213 441 315 152 83 171 194
Labelled Cell Count 546 409 149 190 176 270 417 279 302 342 329 148 233 289 356 427 271 441 315 152 83 171 194


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 5.5 5.4 5.3 5.7 4.6 4.6 4.0 4.1 3.8 3.9 5.8 5.9 5.7 8.7 8.9 9.1 4.8 5.4 5.5
Std Deviation (1e-4) 0.7 0.9 0.8 1.4 1.5 1.1 1.0 0.9 1.2 1.1 1.1 1.2 1.2 1.4 1.8 2.0 2.6 1.4 1.5 1.5
Intensity Change (Log2) -0.05 0.06 -0.25 -0.23 -0.43 -0.39 -0.51 -0.49 0.09 0.12 0.07 0.69 0.72 0.75 -0.19 -0.0 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0.7 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck -1.9 -1.7 0 0 0 0 0 0 -1.7 0 -3.3 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 -0.7 -1.5 -1.9 -1.4 -0.9 -1.0 -2.5 2.3 2.4 2.5 -2.4 -1.9 -2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 1.1 1.3 3.0 1.0 1.6 2.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1.9 2.5 2.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 2.4 3.4 4.0 3.7
SpindlePole -0.4 0.9 0 0 0 0 0 0 -2.5 -2.2 -3.1 0.5 0.9 0.7
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4931 0.4294 0.268 0.2038 0.4585 0.1734 0.0922 0.4495 0.4511 0.4483 0.2324 0.5343 0.454 0.1198 0.0759 0.3301 0.4377 0.0401
Actin 0.0012 0.0015 0.0008 0.0015 0.0005 0.0007 0.0087 0.0004 0.0057 0.0009 0.0031 0.0017 0.027 0.0019 0.0184 0.0001 0.0002 0.0003
Bud 0.0001 0.0022 0.0004 0.0001 0.0005 0.0001 0.0026 0.0015 0.0017 0.0001 0.0025 0.0004 0.0077 0.0024 0.0015 0.0002 0.0001 0
Bud Neck 0.0012 0.0045 0.0126 0.0653 0.0094 0.0051 0.0022 0.0042 0.0288 0.0972 0.0034 0.0065 0.0272 0.0011 0.0029 0.0026 0.0002 0.0007
Bud Periphery 0.0001 0.0019 0.0004 0.0001 0.0011 0.0001 0.0024 0.0019 0.0009 0.0002 0.0038 0.0008 0.006 0.0048 0.0021 0.0001 0.0001 0
Bud Site 0.0023 0.0244 0.0078 0.0018 0.0014 0.001 0.0037 0.0243 0.0045 0.0005 0.0099 0.0009 0.0156 0.008 0.0091 0.0003 0.0002 0.0002
Cell Periphery 0 0.0002 0.0001 0 0.0001 0 0.0003 0.0002 0.0001 0 0.0001 0.0001 0.001 0.0001 0.0001 0 0 0
Cytoplasm 0.0066 0.016 0.0022 0.0023 0.0013 0.0042 0.0066 0.0174 0.0026 0.0014 0.0019 0.013 0.0048 0.0416 0.0044 0.0005 0.0004 0.0062
Cytoplasmic Foci 0.3086 0.1307 0.1863 0.2895 0.2875 0.1688 0.2676 0.1863 0.1315 0.2551 0.2244 0.1469 0.3122 0.2914 0.2859 0.3476 0.5318 0.2813
Eisosomes 0 0.0001 0 0.0001 0.0002 0 0.0016 0.0001 0.0001 0 0 0.0001 0.0005 0.0001 0.0001 0 0 0
Endoplasmic Reticulum 0.0002 0.0017 0.0003 0 0.0001 0.0002 0.0018 0.0007 0.0008 0.0015 0.0002 0.0006 0.0037 0.0019 0.0003 0 0.0001 0.0001
Endosome 0.0069 0.0246 0.0058 0.0069 0.0045 0.0123 0.0163 0.0147 0.0064 0.0131 0.0055 0.0106 0.0253 0.017 0.0062 0.0007 0.0036 0.0089
Golgi 0.0021 0.0041 0.0019 0.0031 0.0036 0.0019 0.0036 0.0031 0.0026 0.003 0.0041 0.0021 0.014 0.0041 0.0023 0.0009 0.0034 0.0026
Lipid Particles 0.032 0.0458 0.0234 0.0211 0.0702 0.0248 0.0378 0.0266 0.0127 0.0367 0.0505 0.0152 0.0445 0.047 0.0128 0.0047 0.0844 0.0415
Mitochondria 0.0001 0.0011 0.0002 0.0001 0.0007 0.0004 0.0101 0.0005 0.0029 0.0002 0.001 0.0014 0.0133 0.0079 0.0016 0.0002 0.0002 0
None 0.053 0.0768 0.0204 0.0241 0.0417 0.0431 0.0343 0.063 0.0204 0.0202 0.0451 0.0602 0.004 0.0622 0.0115 0.0007 0.0006 0.0147
Nuclear Periphery 0.0004 0.0045 0.001 0.0003 0.0006 0.0012 0.0043 0.0023 0.0026 0.0115 0.0012 0.0048 0.0107 0.0012 0.0004 0.0002 0.0003 0.0003
Nucleolus 0.0085 0.0322 0.0178 0.0011 0.0097 0.0098 0.0021 0.0199 0.0146 0.0083 0.005 0.013 0.0039 0.0053 0.0016 0.0015 0.0008 0.001
Nucleus 0.0069 0.0234 0.008 0.0005 0.0012 0.0067 0.0023 0.0139 0.01 0.0069 0.0017 0.0076 0.0088 0.0014 0.001 0.0006 0.0001 0.0005
Peroxisomes 0.0387 0.0417 0.0228 0.0298 0.1214 0.0184 0.0284 0.0284 0.0214 0.012 0.0916 0.0152 0.0297 0.0263 0.0154 0.0063 0.0341 0.0124
Punctate Nuclear 0.5307 0.559 0.6865 0.5522 0.4427 0.7004 0.5613 0.5856 0.7274 0.5286 0.5431 0.6973 0.4288 0.4717 0.6217 0.6327 0.3392 0.6292
Vacuole 0.0003 0.003 0.0011 0.0001 0.0013 0.0005 0.0014 0.0048 0.0019 0.0022 0.0015 0.0014 0.0093 0.0024 0.0007 0 0.0001 0.0001
Vacuole Periphery 0 0.0007 0.0001 0 0.0001 0.0001 0.0004 0.0003 0.0004 0.0005 0.0002 0.0004 0.0021 0.0002 0.0001 0 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.0362 13.1517 1.6972 3.1032 7.8787 3.8358 9.3951 6.6337 4.0738 10.5082
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
432 662 1443 304 2079 1878 402 177 2511 2540 1845 481

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 571.77 710.56 960.44 885.01 713.04 714.83 933.62 892.52 688.74 713.72 954.60 887.77
Standard Deviation 69.42 119.87 119.39 131.43 81.84 96.09 110.64 126.23 96.01 102.84 118.05 129.59
Intensity Change Log 2 0.313522 0.748261 0.630259 0.003617 0.388852 0.323901 0.149802 0.559927 0.468319

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001608 0.000688 0.000358 0.002062 0.000158 0.000354 0.000399 0.000735 0.000407 0.000441 0.000367 0.001574
Bud Neck 0.012784 0.008924 0.005315 0.019374 0.009412 0.018346 0.008413 0.004423 0.009992 0.015890 0.005990 0.013872
Bud Site 0.010862 0.021989 0.022297 0.063107 0.011959 0.021115 0.041497 0.046599 0.011771 0.021343 0.026480 0.057032
Cell Periphery 0.000387 0.000237 0.000074 0.000110 0.000152 0.000158 0.000082 0.000076 0.000192 0.000179 0.000076 0.000098
Cytoplasm 0.081623 0.091583 0.026192 0.025463 0.069158 0.104863* 0.041164 0.020433 0.071303 0.101402* 0.029454 0.023612
Cytoplasmic Foci 0.538583* 0.418759* 0.054373 0.130824 0.622023* 0.487092* 0.114496 0.205565* 0.607668* 0.469282* 0.067473 0.158327
Eisosomes 0.000129 0.000087 0.000021 0.000041 0.000050 0.000069 0.000032 0.000023 0.000064 0.000074 0.000024 0.000034
Endoplasmic Reticulum 0.000744 0.000534 0.000783 0.000342 0.000222 0.000385 0.000452 0.000194 0.000312 0.000424 0.000711 0.000287
Endosome 0.024560 0.052390 0.007282 0.009794 0.037819 0.054697 0.006854 0.013643 0.035538 0.054096 0.007189 0.011210
Golgi 0.021317 0.016687 0.001302 0.003178 0.014143 0.018779 0.001410 0.006616 0.015377 0.018234 0.001325 0.004443
Lipid Particles 0.065315 0.024559 0.003060 0.001438 0.025367 0.019279 0.005764 0.003030 0.032239 0.020655 0.003649 0.002023
Mitochondria 0.010953 0.005271 0.000251 0.000617 0.002082 0.010085 0.000114 0.000757 0.003608 0.008830 0.000221 0.000669
Mitotic Spindle 0.021281 0.171112* 0.819588* 0.684782* 0.080744 0.134774* 0.704277* 0.643036* 0.070514 0.144245* 0.794463* 0.669420*
None 0.016500 0.005611 0.000715 0.002897 0.005664 0.003836 0.000768 0.000115 0.007528 0.004298 0.000727 0.001873
Nuclear Periphery 0.000444 0.000340 0.000382 0.000284 0.000218 0.000322 0.000413 0.000217 0.000257 0.000327 0.000389 0.000259
Nuclear Periphery Foci 0.040719 0.028532 0.012582 0.006594 0.031351 0.012329 0.022995 0.005048 0.032963 0.016552 0.014851 0.006025
Nucleolus 0.022786 0.006971 0.002556 0.003107 0.009012 0.011528 0.003404 0.000824 0.011382 0.010340 0.002741 0.002267
Nucleus 0.029315 0.013546 0.015531 0.006704 0.007191 0.012065 0.007979 0.000607 0.010997 0.012451 0.013886 0.004461
Peroxisomes 0.097562 0.119094* 0.019039 0.022678 0.070272 0.074536 0.022102 0.035500 0.074967 0.086149 0.019706 0.027396
Vacuole 0.002185 0.011497 0.008088 0.015754 0.002741 0.013122 0.017232 0.012064 0.002645 0.012699 0.010080 0.014396
Vacuole Periphery 0.000341 0.001591 0.000211 0.000852 0.000260 0.002264 0.000151 0.000497 0.000274 0.002089 0.000198 0.000721

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.44 0.49 -0.46 -1.05 -1.10 -3.84 -5.48 -3.68 -2.68 -0.94 -0.97 -2.81 -1.95 -1.74 -1.24
Bud Neck 0.31 -0.13 -2.28 -2.69 -2.47 -7.86 -2.27 -0.31 6.99 2.01 -7.11 -3.03 -3.08 0.32 -1.81
Bud Site -4.27 -8.04 -7.29 -5.55 -4.18 -7.58 -7.55 -7.22 -5.67 -1.56 -8.87 -12.53 -10.04 -7.88 -4.78
Cell Periphery 1.99 4.18 3.88 2.92 -0.75 -1.68 1.53 0.96 1.98 0.00 -0.23 4.33 3.05 3.42 -0.59
Cytoplasm -2.28 3.55 1.54 3.81 -1.54 -9.18 0.73 1.40 6.73 0.61 -9.23 4.14 1.53 8.04 -1.13
Cytoplasmic Foci 6.72 28.49 20.62 16.67 -3.54 7.99 38.66 17.46 13.76 -3.81 10.96 65.59 32.96 25.64 -5.71
Eisosomes 4.27 10.72 8.27 4.60 -1.01 -0.21 0.98 6.13 5.83 3.06 1.30 9.06 6.69 5.50 0.35
Endoplasmic Reticulum 1.02 -3.41 -0.02 -1.08 3.44 -2.15 -4.01 -0.69 0.40 3.44 -1.44 -6.94 -2.53 -1.92 4.67
Endosome -6.57 4.09 3.57 8.60 0.59 -6.91 8.14 6.06 10.45 0.37 -8.56 11.67 9.05 15.28 0.53
Golgi 3.20 11.30 10.29 8.82 -0.71 -1.89 15.67 3.96 4.73 -0.88 -0.54 19.76 10.45 10.37 -1.15
Lipid Particles 4.57 8.25 8.52 8.80 4.03 2.21 9.42 12.08 10.33 1.02 4.16 14.70 15.64 14.52 2.93
Mitochondria 0.96 2.42 2.35 3.28 -1.11 -5.84 4.21 1.50 6.21 -0.80 -4.70 3.73 3.13 7.15 -1.15
Mitotic Spindle -11.53 -60.11* -26.02* -17.56 3.78 -2.16 -27.74* -15.48 -14.88 1.83 -6.68 -64.10 -28.07 -25.24 4.69
None 3.63 5.00 4.36 1.97 -1.55 -0.26 5.88 8.71 8.74 2.58 2.28 8.32 5.75 4.50 -1.04
Nuclear Periphery 0.21 -4.11 -2.79 -3.39 0.43 -1.85 -4.74 -1.97 -0.82 2.70 -1.54 -8.02 -4.25 -3.53 1.65
Nuclear Periphery Foci 4.00 5.83 7.48 4.89 3.90 11.84 4.88 9.66 2.14 3.58 11.72 10.07 13.80 4.52 5.20
Nucleolus 3.75 4.67 3.48 0.20 -0.85 -1.81 2.61 4.85 5.84 2.09 0.65 6.13 3.94 3.55 0.15
Nucleus 1.33 -0.59 0.17 -1.04 0.75 -5.33 -2.88 2.69 7.13 3.56 -3.25 -5.63 -1.58 -0.26 2.18
Peroxisomes -2.11 9.79 9.48 13.73 0.59 -2.67 9.73 4.93 6.30 -1.44 -4.33 16.84 11.97 15.11 -0.75
Vacuole -5.06 -8.58 -4.58 -3.06 -2.27 -7.69 -4.62 -3.33 -2.48 -1.11 -9.19 -9.69 -5.65 -4.10 -2.61
Vacuole Periphery -2.81 -0.55 -1.21 1.01 -1.13 -3.73 -1.77 -1.47 2.95 -0.98 -4.36 -2.39 -1.70 2.52 -1.33