Standard name
Human Ortholog
Description Poly(A+) RNA-binding protein; key surveillance factor for the selective export of spliced mRNAs from the nucleus to the cytoplasm; preference for intron-containing genes; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; relocalizes to the cytosol in response to hypoxia; GBP2 has a paralog, HRB1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.07 0.05 0.07 0.06 0.05 0.07 0.05 0.06 0.08 0.14 0.18 0.15 0.11 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0.14 0 0.08 0.12 0.4 0.58 0.43 0.7 0.67 0.76 0.79 0 0 0 0 0.05 0.08 0 0 0 0 0 0
Nucleus 0.92 0.89 0.94 0.88 0.85 0.81 0.68 0.73 0.63 0.59 0.56 0.53 0.89 0.83 0.78 0.74 0.81 0.72 0.89 0.85 0.87 0.86 0.78 0.71
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0 0 0 0 0.06 0.05 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.09 0 0.08 0 0 0.1 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 1 0 0
Bud 1 0 0 0 2 7 16 10 9 11 14 17 0 0 0 0 0 0 0 6 4 1 2 4
Bud Neck 0 0 1 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 0 0 4 10 17 26 17 27 19 27 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 5 14 20 32 26 48 43 31 53 24 35 19 48 72 24 16 24 1 0 0 4 6 3
Endoplasmic Reticulum 0 0 2 0 1 0 1 0 0 0 0 0 1 1 0 1 2 7 0 0 0 0 0 0
Endosome 0 0 1 0 1 0 1 0 0 0 0 1 3 2 4 3 2 1 1 0 5 1 4 3
Golgi 0 0 0 1 1 0 0 0 0 0 0 0 1 0 1 0 0 1 1 0 5 0 0 1
Mitochondria 34 32 12 26 54 202 408 334 403 474 378 469 2 2 1 5 7 11 8 2 2 2 4 2
Nucleus 231 206 438 282 372 412 478 568 362 419 279 313 221 282 316 115 116 104 225 201 423 218 210 191
Nuclear Periphery 4 0 2 3 2 8 10 13 4 5 3 7 1 3 1 0 1 1 0 0 1 1 0 2
Nucleolus 20 4 7 13 14 30 36 46 29 31 15 18 5 2 8 3 3 3 8 1 18 5 11 14
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 1 0 2 0 8 6 4 1 5 0 5 5 1 0 0 1 1 2 3 0 5
Vac/Vac Membrane 0 7 1 9 7 12 9 14 12 23 12 25 0 2 10 12 9 13 2 18 14 11 27 36
Unique Cell Count 250 231 468 319 437 510 698 780 572 710 496 592 247 339 407 155 144 144 253 237 486 254 271 269
Labelled Cell Count 295 254 478 355 491 709 1025 1063 873 1047 745 917 253 348 419 164 156 165 253 237 486 254 271 269


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 6.6 6.6 5.9 5.6 4.4 4.1 3.9 3.6 3.5 3.1 3.1 8.1 7.5 7.3 8.5 8.5 8.2 8.0 8.5 8.8
Std Deviation (1e-4) 1.2 1.7 1.5 1.7 2.1 1.7 1.5 1.4 1.2 1.3 1.0 0.8 1.6 1.6 1.7 2.0 1.7 2.0 1.7 1.8 1.6
Intensity Change (Log2) -0.16 -0.23 -0.59 -0.69 -0.74 -0.88 -0.9 -1.09 -1.09 0.3 0.2 0.16 0.37 0.36 0.31 0.28 0.37 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.2 3.0 1.7 2.9 2.1 1.9 3.2 1.5 2.2 2.8 5.9 7.3 5.6 3.9 5.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.6 5.7 0 0 0 0 0 0 0 0 -2.1 -2.8 0 0 2.8
Nucleus -2.6 -4.2 -5.9 -10.2 -9.0 -11.5 -13.0 -13.3 -14.5 -1.9 -4.7 -6.8 -6.6 -4.7 -7.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.3 1.7 0 0 0 0 0 0 0 0 0 0.5 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.9 0 2.5 2.7 3.6 3.0 4.2 0 0 3.0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.6029 5.1382 4.9114 4.9157 3.8619 4.906 1.8209 3.2973 2.3766 2.1674 2.5292 2.2806 2.0702 2.6172 2.0138 2.0309 1.7183 1.9578
Actin 0.0128 0.0202 0.004 0 0 0 0.0101 0.0083 0.0215 0.0484 0 0.0226 0.0185 0 0.0058 0.0439 0.0003 0.0007
Bud 0.0002 0 0.0001 0 0.0001 0 0.0009 0.0001 0.0003 0.0013 0 0.001 0.0006 0.0001 0.0001 0.0002 0.0002 0.0007
Bud Neck 0.0006 0.0002 0.0003 0 0.0008 0.0005 0.001 0.0001 0.0003 0.0004 0.0004 0.0016 0.0027 0 0.0002 0.0003 0.0009 0.0006
Bud Periphery 0.0007 0.0001 0.0002 0 0 0 0.0009 0.0001 0.0003 0.002 0 0.0017 0.0013 0 0.0001 0.0003 0.0003 0.0008
Bud Site 0.0009 0.0006 0.001 0 0.0001 0 0.0146 0.0006 0.0029 0.0029 0.0001 0.0008 0.0047 0 0.0009 0.0009 0.0007 0.0034
Cell Periphery 0.0003 0 0.0006 0 0 0 0.0004 0 0.0001 0.0002 0 0.0001 0.0005 0 0 0.0001 0.0002 0.0001
Cytoplasm 0.0019 0.0001 0.0001 0 0.0006 0.0001 0.0533 0.0002 0.0074 0.0064 0.0001 0.0009 0.0056 0.0002 0.0028 0.0021 0.0005 0.0002
Cytoplasmic Foci 0.0048 0.0003 0 0 0 0 0.0394 0.0021 0.0152 0.0215 0.0617 0.0016 0.0217 0.0001 0.0115 0.0041 0.0001 0.0007
Eisosomes 0.0001 0.0003 0.0046 0 0 0 0.0002 0 0.0001 0.0002 0 0.0001 0.0004 0 0 0.0003 0 0
Endoplasmic Reticulum 0.0009 0 0.0001 0 0 0 0.0056 0 0.0008 0.0065 0 0.0018 0.0033 0 0.0036 0.0015 0.0002 0.0002
Endosome 0.0147 0.0001 0.0001 0 0.0001 0 0.0858 0.0005 0.0098 0.0123 0.0047 0.0114 0.0167 0 0.0079 0.0077 0.0003 0.0019
Golgi 0.0057 0.0002 0.0001 0 0 0 0.0174 0.001 0.0031 0.0072 0.0034 0.0039 0.0057 0 0.0048 0.004 0.0001 0.0005
Lipid Particles 0.0124 0.0004 0.0001 0 0 0 0.0354 0.0008 0.0036 0.012 0.0557 0.0013 0.0168 0 0.0045 0.008 0.0001 0.0015
Mitochondria 0.0028 0.0031 0.003 0 0.0002 0.0001 0.0072 0.0006 0.0016 0.0858 0.0006 0.023 0.0176 0.0002 0.0017 0.0055 0.0025 0.0013
None 0.0012 0 0.0002 0 0.0002 0 0.0188 0.0001 0.0015 0.0018 0 0.0003 0.014 0 0.0005 0.0006 0.0002 0.0001
Nuclear Periphery 0.0144 0.0011 0.0004 0.0002 0.0013 0.0004 0.0328 0.0005 0.0023 0.0414 0.0018 0.0119 0.0189 0.0007 0.0031 0.0139 0.0023 0.0009
Nucleolus 0.0265 0.0085 0.012 0.0042 0.0418 0.0333 0.0156 0.0103 0.0049 0.0051 0.0109 0.0287 0.0176 0.0106 0.0086 0.0042 0.0627 0.0196
Nucleus 0.8588 0.9584 0.9724 0.9359 0.9492 0.9632 0.5899 0.9699 0.9134 0.7003 0.82 0.8822 0.7903 0.9869 0.9303 0.86 0.9244 0.9653
Peroxisomes 0.0204 0.0059 0.0001 0 0 0 0.0117 0.0037 0.0055 0.0283 0.0386 0.0009 0.0123 0 0.0105 0.0015 0.0001 0.0007
Punctate Nuclear 0.0144 0.0002 0.0006 0.0595 0.0054 0.0022 0.0507 0.0007 0.0025 0.0067 0.0016 0.0018 0.0224 0.001 0.0016 0.0395 0.0028 0.0006
Vacuole 0.0042 0.0002 0.0001 0 0.0001 0.0001 0.0059 0.0001 0.0022 0.0045 0.0002 0.0011 0.0051 0 0.001 0.0004 0.0007 0.0002
Vacuole Periphery 0.0012 0.0001 0 0 0 0 0.0025 0.0001 0.0009 0.0049 0.0001 0.0014 0.0033 0 0.0004 0.0009 0.0004 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 69.1776 80.6616 129.3995 100.8297 97.4911 74.722 84.6638 95.6741 103.0292 88.3592
Translational Efficiency 1.2963 1.4886 0.8618 1.2489 1.0653 1.3711 1.1678 1.3981 1.0678 1.1132

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2209 343 1473 1255 1692 1900 163 71 3901 2243 1636 1326

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 836.59 1020.58 956.57 1029.35 787.77 910.96 1067.21 1087.62 815.42 927.72 967.59 1032.47
Standard Deviation 112.02 153.45 136.92 152.20 113.05 127.07 135.89 151.28 115.04 137.24 140.77 152.71
Intensity Change Log 2 0.286797 0.193350 0.299141 0.209613 0.437998 0.465328 0.249880 0.317181 0.382129

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000227 0.001024 0.000706 0.001080 0.000307 0.000457 0.001196 0.001786 0.000262 0.000544 0.000755 0.001118
Bud Neck 0.006262 0.013898 0.017044 0.024279 0.005062 0.012608 0.006686 0.010241 0.005741 0.012805 0.016012 0.023527
Bud Site 0.000478 0.001246 0.001077 0.004838 0.000997 0.001328 0.000921 0.005042 0.000703 0.001315 0.001061 0.004849
Cell Periphery 0.000069 0.000059 0.000058 0.000193 0.000098 0.000109 0.000044 0.000132 0.000082 0.000101 0.000056 0.000190
Cytoplasm 0.002299 0.000662 0.000569 0.005786 0.001853 0.001610 0.000869 0.007299 0.002105 0.001465 0.000599 0.005867
Cytoplasmic Foci 0.000171 0.000779 0.000013 0.000625 0.000389 0.000699 0.000056 0.000056 0.000265 0.000711 0.000017 0.000595
Eisosomes 0.000016 0.000037 0.000030 0.000032 0.000018 0.000026 0.000051 0.000037 0.000017 0.000027 0.000032 0.000032
Endoplasmic Reticulum 0.003437 0.006739 0.003763 0.006323 0.003800 0.005023 0.007374 0.008807 0.003595 0.005285 0.004123 0.006456
Endosome 0.000225 0.000504 0.000128 0.001525 0.000356 0.000979 0.000166 0.000509 0.000282 0.000906 0.000132 0.001471
Golgi 0.000170 0.001686 0.000058 0.002055 0.000310 0.000448 0.000071 0.000112 0.000231 0.000637 0.000059 0.001951
Lipid Particles 0.000163 0.000277 0.000047 0.000159 0.000367 0.000593 0.000217 0.000336 0.000252 0.000545 0.000064 0.000168
Mitochondria 0.000988 0.002035 0.001180 0.003419 0.000672 0.003134 0.001048 0.002246 0.000851 0.002966 0.001167 0.003356
Mitotic Spindle 0.002119 0.010153 0.000516 0.011766 0.000941 0.006142 0.000102 0.009458 0.001608 0.006756 0.000475 0.011643
None 0.002227 0.001716 0.001404 0.004476 0.003091 0.001737 0.001994 0.006603 0.002602 0.001733 0.001463 0.004590
Nuclear Periphery 0.000582 0.000349 0.000361 0.002032 0.000389 0.000939 0.000288 0.000983 0.000498 0.000849 0.000354 0.001976
Nuclear Periphery Foci 0.000227 0.000471 0.000058 0.001119 0.000309 0.000422 0.000086 0.000170 0.000263 0.000429 0.000061 0.001068
Nucleolus 0.015841 0.021607 0.008637 0.007916 0.012265 0.018842 0.009790 0.003640 0.014290 0.019264 0.008752 0.007687
Nucleus 0.963825* 0.934268* 0.963440* 0.910095* 0.967984* 0.942015* 0.965987* 0.936421* 0.965629* 0.940830* 0.963694* 0.911505*
Peroxisomes 0.000195 0.001594 0.000202 0.000248 0.000441 0.000764 0.000432 0.000247 0.000302 0.000891 0.000224 0.000248
Vacuole 0.000324 0.000649 0.000595 0.008023 0.000235 0.001269 0.002496 0.005671 0.000285 0.001174 0.000785 0.007897
Vacuole Periphery 0.000157 0.000248 0.000112 0.004011 0.000117 0.000858 0.000126 0.000205 0.000140 0.000765 0.000114 0.003807

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.03 -15.20 -17.22 -2.39 -7.84 -2.41 -8.56 -6.29 -5.95 -3.36 -7.38 -13.88 -16.75 -12.60 -8.01
Bud Neck -4.31 -12.40 -14.38 -5.65 -5.30 -8.24 -3.69 -3.94 -1.40 -2.35 -8.79 -13.73 -15.36 -9.04 -5.84
Bud Site -4.33 -5.74 -6.04 -4.78 -4.81 -0.69 -0.80 -2.33 -2.21 -2.20 -2.14 -2.75 -5.91 -5.01 -5.14
Cell Periphery 0.82 1.05 -2.66 -2.94 -3.02 -0.28 2.07 -2.08 -1.38 -3.68 -0.66 2.17 -2.33 -1.13 -3.26
Cytoplasm 3.35 3.48 -4.69 -7.71 -7.84 0.20 1.79 -2.73 -2.74 -3.01 0.94 4.62 -5.80 -5.79 -8.29
Cytoplasmic Foci -1.26 2.75 -1.23 0.88 -2.65 -0.55 1.95 1.74 2.07 -1.66 -1.45 2.85 -0.04 1.40 -2.65
Eisosomes -7.38 -12.87 -14.46 -0.42 -3.79 -4.87 -8.79 -5.16 -4.37 -1.02 -8.57 -14.48 -14.83 -6.81 -3.15
Endoplasmic Reticulum -4.73 -2.91 -11.07 -0.39 -9.42 -4.07 -5.92 -5.56 -4.76 -2.53 -6.58 -4.58 -12.01 -5.59 -8.99
Endosome -0.99 2.67 -3.63 -1.39 -3.98 -1.86 0.90 -2.69 -0.01 -4.20 -2.35 1.82 -3.40 -0.32 -4.16
Golgi -1.24 0.92 -1.57 0.46 -1.78 -0.60 1.41 0.37 1.30 -4.24 -1.71 1.74 -1.43 -0.59 -1.79
Lipid Particles -0.95 1.77 -1.44 0.26 -7.40 -0.69 0.66 -1.07 -0.14 -2.54 -1.30 2.05 -0.11 1.35 -7.37
Mitochondria -2.07 -1.55 -6.92 -2.69 -7.38 -3.61 -2.92 -3.52 0.01 -2.78 -3.61 -2.96 -8.83 -0.75 -7.77
Mitotic Spindle -1.66 1.51 -5.56 -1.36 -6.27 -3.48 1.34 -2.35 -2.07 -2.39 -3.54 1.42 -6.31 -3.90 -6.71
None 1.11 2.04 -2.75 -3.51 -4.30 2.52 1.43 -1.47 -2.34 -2.05 2.41 3.27 -2.41 -4.01 -4.54
Nuclear Periphery 2.22 1.53 -8.17 -9.16 -8.99 -3.14 -0.16 -5.76 -3.73 -5.74 -2.38 0.80 -9.23 -6.18 -9.49
Nuclear Periphery Foci -0.89 1.75 -2.08 -0.93 -2.74 -0.74 1.54 -1.60 -1.27 -2.49 -1.48 2.43 -2.07 -1.40 -2.88
Nucleolus -1.86 5.37 4.90 4.07 0.25 -5.03 1.49 0.65 2.23 -0.05 -4.15 5.43 4.58 7.46 0.23
Nucleus 3.96 2.14 12.33 3.90 11.56 6.90 2.23 4.43 3.28 4.02 7.76 3.42 13.65 7.55 12.22
Peroxisomes -1.54 -0.57 -1.39 1.45 -1.67 -0.88 0.36 0.54 1.48 0.44 -2.09 0.95 0.64 2.44 -1.15
Vacuole -3.10 -3.77 -7.86 -7.54 -7.44 -4.75 -3.05 -5.11 -4.73 -3.08 -4.78 -4.98 -8.46 -7.64 -7.60
Vacuole Periphery -1.55 1.22 -2.91 -2.77 -2.97 -2.67 -0.76 -3.22 1.98 -2.73 -2.64 0.91 -2.95 -1.85 -2.98