Standard name
Description Potassium transporter that mediates K+ influx; activates high-affinity Ca2+ influx system (HACS) during mating pheromone response; expression up-regulated in response to alpha factor; localized to sites of polarized growth; member of a fungal-specific gene family; potential Cdc28p substrate; KCH1 has a paralog, PRM6, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.66 0.9 0.88 0.95 0.92 0.85 0.82 0.83 0.79 0.8 0.76 0.96 0.97 0.97 0.79 0.79 0.69 0.31 0.46 0.37 0.2 0.1 0.06
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0.05 0.05 0
Endosome 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.11 0.09 0.13 0.07 0.08 0.09 0.16 0.2 0.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.08
Mitochondria 0.05 0 0 0 0 0.2 0.21 0.3 0.32 0.34 0.39 0 0 0 0.05 0 0.06 0.07 0.11 0.18 0.23 0.14 0.1
Nucleus 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0 0.05 0 0 0.06 0.05 0 0 0 0 0 0 0 0.09 0.13 0.14 0.47 0.23 0.2 0.2 0.32 0.41
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 2 0 0 2 0 0 1 1 2 3 1 3 0 1 1 9 11 8 16 18
Bud 0 4 5 3 0 17 19 14 17 22 29 1 0 1 1 0 1 2 11 11 12 26 25
Bud Neck 0 0 1 2 1 0 5 0 0 1 0 2 7 5 2 4 0 1 2 4 3 4 5
Bud Site 1 0 1 0 1 1 0 0 1 3 1 0 0 0 0 0 0
Cell Periphery 3 2 1 3 2 0 0 0 2 0 2 0 4 0 0 0 0 0 1 2 0 0 0
Cytoplasm 126 384 391 338 407 434 626 338 376 433 537 200 438 344 227 220 159 83 212 164 67 55 32
Endoplasmic Reticulum 4 13 2 0 0 1 1 0 0 1 0 5 8 5 19 11 10 4 5 12 16 26 15
Endosome 6 1 20 8 18 10 15 2 3 4 3 1 0 3 31 24 29 18 34 39 55 115 114
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 5 4 6 8 20 42 46
Mitochondria 9 17 7 6 11 104 159 122 153 186 274 1 0 0 13 9 13 17 50 79 77 79 57
Nucleus 18 1 2 0 0 2 3 0 2 1 3 0 1 0 3 3 1 0 0 1 0 1 0
Nuclear Periphery 11 4 0 0 0 0 2 0 1 1 0 0 0 0 0 0 0 0 0 1 0 5 1
Nucleolus 1 0 0 0 2 0 2 1 0 2 0 0 0 0 0 0 3 0 0 0 0 0 0
Peroxisomes 0 0 2 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 2
SpindlePole 0 0 6 4 1 3 7 3 5 6 7 0 0 0 6 6 13 0 7 7 4 9 11
Vac/Vac Membrane 35 11 21 8 19 29 36 8 18 12 16 0 3 3 25 35 32 125 104 87 69 185 239
Unique Cell Count 191 427 442 355 441 509 760 406 474 540 706 209 453 354 286 278 230 266 457 444 343 580 580
Labelled Cell Count 214 438 459 375 463 601 878 488 578 673 873 212 464 362 330 313 267 266 457 444 343 580 580


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.6 4.4 3.9 4.2 3.5 3.8 3.6 3.2 3.3 3.2 5.2 5.3 5.6 4.9 5.5 6.3 6.4 7.2 7.6
Std Deviation (1e-4) 0.9 1.0 4.4 1.4 1.3 1.0 1.2 1.1 0.9 1.1 0.8 1.0 0.9 1.1 1.4 1.5 2.1 1.6 1.9 2.0
Intensity Change (Log2) -0.19 -0.08 -0.34 -0.22 -0.31 -0.46 -0.43 -0.45 0.24 0.26 0.35 0.16 0.3 0.51 0.52 0.71 0.77


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 2.2 3.8 4.2


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kch1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available