Standard name
Description Protein involved in retention of membrane proteins; including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.11 0.15 0.08 0 0 0 0 0 0 0 0 0 0.15 0.08 0 0.12 0.12 0.14 0.06 0.16 0.09 0.06 0.08 0.06
Bud 0 0 0 0 0 0 0.05 0.06 0.06 0.06 0.08 0.06 0 0 0 0 0 0.05 0 0 0 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.46 0.5 0.37 0.5 0.56 0.51 0.46 0.47 0.41 0.51 0.46 0.44 0.46 0.49 0.51 0.6 0.63 0.42 0.07 0.12 0.07 0.07 0.08 0.14
Endoplasmic Reticulum 0.33 0.36 0.26 0.1 0 0 0 0 0 0 0 0 0.28 0.36 0.1 0 0 0.08 0.16 0.17 0.16 0.1 0.12 0.15
Endosome 0.09 0.06 0.1 0.11 0.18 0 0.05 0 0 0 0 0 0.15 0.07 0.26 0.21 0.16 0.25 0.19 0.13 0.18 0.13 0.13 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.22 0.2 0.21 0.16 0.11 0.08
Mitochondria 0.05 0 0.36 0.29 0.26 0.52 0.59 0.59 0.67 0.54 0.69 0.66 0 0 0 0.08 0 0.09 0.19 0.13 0.2 0.29 0.32 0.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.06 0 0.12 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.07 0.08 0.05 0 0 0.07 0.08 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 11 24 11 5 4 4 0 2 0 0 0 3 19 15 4 15 10 14 10 33 14 7 12 11
Bud 2 0 4 8 8 12 27 29 22 34 28 37 1 2 2 1 3 5 1 2 0 6 3 3
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 1 1 1 0 0 0 3 3 1 1 0 2
Bud Site 0 0 0 0 0 0 3 2 5 9 2 5 0 0 0 0 0 0
Cell Periphery 2 0 0 0 1 2 0 2 2 1 1 4 0 1 0 2 0 1 0 0 0 0 0 0
Cytoplasm 44 81 48 113 171 148 236 232 154 282 162 266 57 88 66 76 52 43 11 25 11 8 12 25
Endoplasmic Reticulum 32 59 34 23 11 12 11 2 1 3 4 19 34 64 13 5 3 8 27 36 27 11 18 27
Endosome 9 10 13 25 55 8 27 13 4 18 1 0 18 12 34 27 13 26 31 28 29 16 20 16
Golgi 1 5 3 7 0 0 0 0 0 0 0 0 0 9 3 0 1 1 36 42 34 19 17 15
Mitochondria 5 0 47 65 79 151 298 289 249 296 244 404 1 1 1 10 3 9 30 27 32 35 50 54
Nucleus 0 0 0 0 0 0 2 3 1 3 2 4 0 0 0 0 0 0 0 0 1 0 0 0
Nuclear Periphery 0 1 0 0 0 1 1 1 1 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 0 0 2 0 1 1 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 5 5 0 4 4 2 6 0 2 1 0 1 0 0 0 2 1 5 4 2 2
SpindlePole 1 0 1 7 6 3 14 15 15 26 4 21 2 2 4 8 3 12 2 1 1 0 1 1
Vac/Vac Membrane 1 1 0 1 7 0 6 9 4 11 5 5 5 2 11 2 6 8 7 6 2 8 12 15
Unique Cell Count 96 162 130 226 303 290 508 491 373 551 356 608 123 178 130 127 83 103 167 213 165 123 157 180
Labelled Cell Count 108 181 161 259 349 341 631 604 461 689 454 770 139 198 141 146 94 127 167 213 165 123 157 180


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 8.1 5.6 5.0 5.3 4.3 3.9 3.8 3.4 3.5 3.2 3.3 7.2 7.2 7.2 7.3 7.8 7.3 7.9 8.0 7.3
Std Deviation (1e-4) 1.0 1.6 1.2 1.3 2.0 1.2 1.3 1.2 0.9 0.9 0.8 0.7 1.5 1.4 1.3 1.2 1.4 1.3 1.8 1.8 1.4
Intensity Change (Log2) -0.16 -0.08 -0.37 -0.53 -0.57 -0.69 -0.69 -0.79 -0.75 0.36 0.38 0.36 0.4 0.49 0.39 0.5 0.52 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.7 0 0 0 0 0 0 0 0 1.7 0 -1.9 0.9 0.9 1.3
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.4 3.7 2.7 2.0 2.1 0.9 2.9 1.7 1.4 1.5 2.2 2.2 3.7 3.7 0.7
Endoplasmic Reticulum -4.0 -7.0 0 0 0 0 0 0 0 0.3 1.8 -3.4 -5.0 -4.2 -3.6
Endosome 0.3 2.1 0 0 0 0 0 0 0 1.1 -1.0 3.4 2.5 1.2 3.1
Golgi 0 0 0 0 0 0 0 0 0 0 1.2 0 0 0 0
Mitochondria -1.4 -2.1 0 0 0 0 0 0 0 -7.2 -8.5 -7.4 -5.5 -5.5 -4.9
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.6
Vacuole 0 0 0 0 0 0 0 0 0 0 0 3.4 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rer1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available