Standard name
Description Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.14 0.26 0.17 0.21 0 0.15 0.19 0.24 0.15 0.14 0.12 0.18 0.15 0.18 0.12 0.11 0.09 0.12 0.15 0.16 0.13 0.3 0.26 0.31
Bud 0.09 0.1 0.08 0.09 0.07 0.1 0.15 0.13 0.15 0.12 0.14 0.15 0.07 0 0 0 0 0 0.32 0.29 0.23 0.34 0.35 0.28
Bud Neck 0.15 0.12 0.21 0.19 0.28 0.19 0.16 0.17 0.19 0.2 0.18 0.19 0.2 0.24 0.24 0.11 0.19 0.08 0.35 0.33 0.39 0.24 0.19 0.18
Bud Site 0.23 0.12 0.1 0.18 0.17 0.12 0.07 0 0.07 0.05 0 0.05 0.1 0 0 0.05 0.08 0.1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.07 0.09 0.09 0.15 0.11 0.12 0.16 0.12 0.13 0.15 0.17 0.1 0.09 0.09 0.15 0.13 0.1 0 0 0 0 0 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.09 0.09 0.11 0.06 0 0 0 0 0 0
Mitochondria 0.06 0.07 0 0 0 0.09 0.14 0.09 0.19 0.16 0.25 0.14 0 0 0 0.18 0.06 0.14 0 0 0 0 0 0
Nucleus 0 0.06 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.32 0.38 0.32 0.23 0.29 0.34 0.36 0.35 0.31 0.32 0.32 0.33 0.18 0.21 0.21 0.34 0.36 0.43 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0.06 0 0.07 0 0 0 0 0 0 0.09 0.1 0.07 0.06 0.08 0.08 0 0 0 0 0 0
SpindlePole 0 0.05 0.14 0.14 0.13 0.09 0.08 0.07 0.07 0.08 0 0.08 0.12 0.16 0.17 0.05 0.05 0.05 0 0 0.05 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0.06 0.07 0.06 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 13 21 20 29 5 27 37 48 35 21 26 28 21 34 25 17 10 19 24 27 21 112 78 84
Bud 8 8 9 12 9 18 30 26 35 19 29 23 10 7 7 3 3 6 51 50 37 125 107 78
Bud Neck 14 10 25 26 38 35 32 35 45 30 37 30 29 45 49 16 20 12 56 57 62 89 57 49
Bud Site 22 10 12 25 23 21 14 9 16 8 7 8 14 8 6 8 9 16 – – – – – –
Cell Periphery 4 1 2 2 1 0 2 8 9 4 3 3 2 7 4 2 0 0 2 0 0 2 2 6
Cytoplasm 8 6 11 13 21 20 23 32 28 20 32 26 15 16 18 22 14 15 4 3 3 9 12 15
Endoplasmic Reticulum 1 0 0 1 0 4 2 3 3 1 9 5 3 4 0 2 0 1 1 0 0 4 6 5
Endosome 4 1 3 3 6 5 6 4 6 2 9 6 4 13 7 4 3 3 1 3 2 1 5 5
Golgi 2 3 4 3 0 1 2 10 2 2 1 1 3 1 18 13 12 10 0 0 0 1 1 3
Mitochondria 6 6 1 5 4 17 28 18 45 25 52 21 2 1 4 27 6 22 1 6 1 4 12 11
Nucleus 2 5 2 1 6 12 4 8 6 5 3 1 3 2 5 7 3 1 0 0 0 0 0 0
Nuclear Periphery 0 2 0 1 0 1 0 0 1 1 1 0 0 0 0 0 0 0 0 2 3 3 3 2
Nucleolus 30 30 37 32 40 61 70 71 73 49 68 51 26 38 43 51 39 66 1 7 4 1 0 0
Peroxisomes 4 2 8 8 1 12 8 5 3 3 5 3 13 19 13 9 9 12 3 2 5 4 5 3
SpindlePole 3 4 16 20 18 16 15 15 16 12 6 12 17 29 35 7 5 8 4 4 8 2 4 3
Vac/Vac Membrane 1 0 2 1 3 4 1 3 9 13 7 7 9 13 11 5 3 6 0 2 0 1 0 2
Unique Cell Count 94 80 117 139 136 180 194 202 232 153 210 154 143 184 200 148 107 154 160 174 160 369 305 277
Labelled Cell Count 122 109 152 182 175 254 274 295 332 215 295 225 171 237 245 193 136 197 160 174 160 369 305 277


Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.7 22.9 15.3 15.5 16.3 15.0 14.6 15.3 13.2 13.7 11.8 13.9 18.7 17.5 18.6 27.5 26.3 25.7 17.6 18.4 18.7
Std Deviation (1e-4) 5.9 10.2 5.8 5.0 6.5 7.5 7.0 6.8 5.6 6.0 5.2 6.7 9.7 7.2 8.7 23.7 12.3 13.6 6.9 5.7 7.0
Intensity Change (Log2) – – – 0.02 0.09 -0.03 -0.07 -0.0 -0.22 -0.16 -0.38 -0.14 0.29 0.19 0.28 0.85 0.78 0.75 0.2 0.26 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.8 -3.6 -0.5 0.4 1.4 -0.5 -0.8 -1.2 0.2 -0.5 0.3 -1.1 -1.3 -1.7 -1.1
Bud 0.3 -0.3 0.7 2.0 1.4 2.0 1.3 1.7 1.8 -0.2 -1.5 -1.6 -2.2 -1.6 -1.4
Bud Neck -0.5 1.2 -0.4 -1.1 -0.9 -0.4 -0.4 -0.8 -0.4 -0.2 0.6 0.6 -2.4 -0.5 -3.2
Bud Site 1.8 1.5 0.4 -0.9 -2.0 -1.1 -1.6 -2.6 -1.6 -0.1 -2.0 -2.7 -1.5 -0.5 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.4 0.5 0.7 1.6 0.7 0.9 1.5 1.8 0.3 -0.2 -0.1 1.3 0.9 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 1.7 0 0 0 0
Golgi 0 0 0 0 0.6 0 0 0 0 0 0 1.9 1.8 2.3 1.1
Mitochondria 0 0 3.0 4.0 2.9 4.8 4.3 5.6 3.8 0 0 0 4.6 0 3.9
Nucleus 0 0 2.0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.5 -0.4 0.4 0.8 0.6 0 0.1 0.1 0.3 -2.5 -2.1 -2.0 0.5 0.8 1.9
Peroxisomes -0.4 0 -0.1 -1.0 0 0 0 0 0 0.7 1.0 -0.1 -0.2 0.4 0.3
SpindlePole 0.2 -0.1 -1.3 -1.7 -1.8 -2.1 -1.6 -3.7 -1.6 -0.4 0.5 0.9 -2.6 -2.3 -2.4
Vacuole 0 0 0 0 0 0 2.4 0 0 0 2.1 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.368 10.3606 12.7014 9.9131 10.9153 11.4126 15.3707 12.3434 13.2009 13.0762 13.1433 13.7896 16.5855 13.2855 14.9946 14.8206 15.0386 15.957
Actin 0.0888 0.0566 0.0387 0.0285 0.0381 0.1486 0.0628 0.0319 0.0188 0.0293 0.028 0.0697 0.0778 0.0454 0.0261 0.0088 0.0063 0.1535
Bud 0.0104 0.037 0.0185 0.0058 0.0013 0.0074 0.0129 0.0243 0.0093 0.0012 0.0056 0.0052 0.0082 0.0296 0.0141 0.0017 0.0037 0.0054
Bud Neck 0.1111 0.036 0.5255 0.7639 0.6395 0.6616 0.2286 0.0525 0.5166 0.7622 0.6891 0.8233 0.1746 0.0456 0.5314 0.7792 0.7913 0.753
Bud Periphery 0.016 0.0438 0.0215 0.0128 0.0016 0.0164 0.0212 0.0192 0.0138 0.0017 0.01 0.0154 0.0091 0.0227 0.0152 0.002 0.0055 0.015
Bud Site 0.3028 0.7462 0.2874 0.0235 0.0046 0.0396 0.4268 0.8188 0.2662 0.021 0.0161 0.0172 0.5031 0.7659 0.2997 0.0118 0.0141 0.0209
Cell Periphery 0.0108 0.0132 0.0122 0.0063 0.0028 0.0093 0.0125 0.0073 0.0108 0.004 0.007 0.0054 0.0086 0.0128 0.0135 0.0091 0.0072 0.0078
Cytoplasm 0.0308 0.0137 0.0038 0.0035 0.0016 0.0016 0.0033 0.0006 0.0016 0.001 0.0025 0.001 0.0044 0.0009 0.0021 0.001 0.001 0.0008
Cytoplasmic Foci 0.1206 0.0127 0.0106 0.0177 0.0664 0.0375 0.0752 0.0137 0.0261 0.0094 0.015 0.0177 0.0599 0.0298 0.0192 0.0281 0.0174 0.0087
Eisosomes 0.0025 0.0011 0.0013 0.0007 0.0007 0.0029 0.0036 0.0008 0.0011 0.0005 0.0031 0.0009 0.0053 0.0007 0.0011 0.0011 0.0009 0.0022
Endoplasmic Reticulum 0.0058 0.001 0.0008 0.0018 0.0026 0.0004 0.0026 0 0.0004 0.0019 0.0026 0.0001 0.0042 0.0001 0.0007 0.0004 0.0006 0.0005
Endosome 0.049 0.0011 0.0077 0.0232 0.0339 0.0054 0.0068 0.0001 0.0168 0.0351 0.0526 0.0008 0.0162 0.0001 0.0017 0.0079 0.0175 0.0016
Golgi 0.0182 0.0007 0.0097 0.0163 0.0198 0.0021 0.0025 0.0002 0.0043 0.0166 0.0109 0.0013 0.0056 0.0008 0.0014 0.0074 0.0042 0.0016
Lipid Particles 0.0339 0.0021 0.0085 0.0343 0.0413 0.0063 0.0168 0.0017 0.0272 0.0536 0.0164 0.0024 0.0119 0.0022 0.0025 0.0228 0.0164 0.0017
Mitochondria 0.0471 0.0026 0.0074 0.0078 0.0334 0.0083 0.0196 0.0023 0.0066 0.0114 0.034 0.0066 0.0334 0.0106 0.0107 0.0263 0.0301 0.0027
None 0.0138 0.0007 0.0004 0.002 0.0016 0.0005 0.0044 0.0003 0.0025 0.0014 0.0021 0.0001 0.0027 0.0004 0.003 0.0035 0.0007 0.0004
Nuclear Periphery 0.0237 0.0007 0.0006 0.0062 0.0302 0.001 0.0017 0.0001 0.001 0.0061 0.0094 0.0002 0.0075 0.0001 0.0003 0.0012 0.001 0.0015
Nucleolus 0.0389 0.0093 0.0252 0.0163 0.0461 0.0156 0.0439 0.0078 0.042 0.0223 0.0487 0.013 0.0207 0.0156 0.0331 0.0591 0.0349 0.0094
Nucleus 0.0112 0.0032 0.0043 0.0046 0.0051 0.0069 0.0074 0.0021 0.006 0.0047 0.0084 0.0025 0.0043 0.0033 0.0062 0.0073 0.0047 0.0019
Peroxisomes 0.0311 0.0021 0.011 0.015 0.0158 0.0083 0.0302 0.0028 0.0121 0.0087 0.0215 0.0083 0.0274 0.0063 0.0063 0.0108 0.03 0.0026
Punctate Nuclear 0.0157 0.001 0.0019 0.0076 0.008 0.0005 0.0068 0.0089 0.0032 0.0039 0.0098 0.0024 0.0044 0.0015 0.0008 0.006 0.0023 0.002
Vacuole 0.0132 0.0132 0.0026 0.0014 0.0023 0.0144 0.0091 0.0034 0.0121 0.0029 0.0048 0.0049 0.0091 0.0048 0.009 0.0024 0.008 0.0059
Vacuole Periphery 0.0045 0.0021 0.0004 0.0009 0.0033 0.0053 0.0014 0.001 0.0016 0.0012 0.0025 0.0014 0.0018 0.0008 0.0019 0.0019 0.0022 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 55.7965 67.1835 62.694 65.1667 53.1497 54.6275 75.9057 72.9394 62.0059 65.3218
Translational Efficiency 1.6275 1.7313 1.5755 1.5897 1.3275 1.6136 1.691 1.2401 1.4801 1.3318

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘