Standard name
Human Ortholog
Description Citrate synthase, peroxisomal isozyme involved in glyoxylate cycle; catalyzes condensation of acetyl coenzyme A and oxaloacetate to form citrate; expression is controlled by Rtg1p and Rtg2p transcription factors; SCF-Ucc1 regulates level of Cit2p to maintain citrate homeostasis; oxaloacetate-dependent positive feedback loop inhibits Cit2p ubiquitination; CIT2 has a paralog, CIT1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.09 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.97 0.96 0.94 0.9 0.88 0.9 0.76 0.79 0.69 0.78 0.97 0.99 0.99 0.95 0.98 0.96 0.77 0.83 0.72 0.73 0.77 0.74
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.07 0.09 0.06 0.09 0.15 0.26 0.16 0.32 0.41 0.47 0.4 0 0 0 0.05 0 0 0.09 0.07 0.15 0 0 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 4 3 1 5 5 8
Bud 3 0 2 1 2 4 4 4 7 7 8 6 10 6 0 0 0 0 3 1 1 22 28 22
Bud Neck 2 1 0 0 0 1 2 4 0 2 0 0 0 0 0 0 0 0 2 1 1 3 3 5
Bud Site 0 0 0 0 0 0 1 0 2 1 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 0 0 0 4 2 1 2 0 1 1 3 7 4 10 3 9 0 0 0 0 0 1 3
Cytoplasm 250 198 116 153 100 181 220 258 161 222 139 236 420 569 620 250 162 101 223 174 82 185 247 340
Endoplasmic Reticulum 1 0 0 1 0 0 0 1 2 0 1 0 0 0 2 21 9 6 9 0 0 3 3 5
Endosome 0 0 0 1 1 2 0 2 0 0 0 0 0 1 1 9 1 1 4 1 1 0 0 5
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1 0 1 1 1 0 0 1 2
Mitochondria 13 14 11 10 10 31 65 46 68 115 95 120 1 0 1 14 4 2 25 15 17 9 8 26
Nucleus 0 1 0 0 0 1 0 2 1 2 4 0 1 1 0 1 1 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 0 1 3 2 9 0 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 1 0 0 1 0 1 0 1 1 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 3 0 0 0 0 2 1 3 1 2 0 4 3 0 0 0 0 1 4 1 0 11 10 8
Vac/Vac Membrane 1 0 0 0 3 2 2 4 4 6 13 1 2 4 3 2 1 3 3 3 2 3 3 14
Unique Cell Count 262 202 119 159 106 201 250 287 212 282 202 301 431 577 627 264 166 105 289 210 115 253 324 460
Labelled Cell Count 278 214 129 168 121 227 296 328 250 361 270 371 445 587 638 302 187 115 289 210 115 253 324 460


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 3.5 3.4 4.5 6.1 3.9 3.0 3.5 3.5 2.7 4.3 2.9 8.6 7.1 6.5 5.3 6.1 6.1 4.8 5.4 5.7
Std Deviation (1e-4) 0.9 0.7 1.4 1.5 2.7 1.2 1.0 1.6 1.5 1.0 1.9 1.3 5.8 4.3 3.0 2.7 2.3 1.9 2.2 2.1 1.9
Intensity Change (Log2) – – – 0.43 0.86 0.21 -0.18 0.06 0.07 -0.32 0.36 -0.23 1.34 1.07 0.95 0.65 0.84 0.86 0.52 0.69 0.75


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.507 18.2794 16.2134 15.957 15.7733 17.384 10.6404 17.0838 12.6151 11.4659 12.1876 13.5054 13.6276 17.875 16.7837 16.5331 15.6913 18.0224
Actin 0.0189 0.0007 0.0027 0.0223 0.0053 0.005 0.0383 0.0017 0.0041 0.0087 0.0038 0.0072 0.0341 0.0043 0.0262 0.0019 0.0059 0.002
Bud 0.0012 0.0006 0.0002 0.0031 0.0007 0.0002 0.0006 0.0003 0.0004 0.0009 0.0001 0.0002 0.0006 0.0022 0.001 0.0001 0 0.0002
Bud Neck 0.0025 0.0002 0.0005 0.0014 0.0027 0.0015 0.0008 0.0004 0.0004 0.0003 0.0004 0.0025 0.002 0.0004 0.0011 0.0005 0.0004 0.0014
Bud Periphery 0.0023 0.0002 0.0001 0.004 0.0006 0.0001 0.0005 0.0001 0.0004 0.0004 0.0001 0.0003 0.0008 0.0006 0.0007 0 0 0.0001
Bud Site 0.0039 0.0012 0.0003 0.0018 0.0006 0.0001 0.0051 0.0039 0.0008 0.001 0.0002 0.0002 0.0031 0.009 0.0034 0.0003 0.0002 0.0001
Cell Periphery 0.0009 0.0004 0.0002 0.0008 0.0002 0.0001 0.0003 0.0003 0.0002 0.0001 0.0001 0.0001 0.0017 0.0006 0.0002 0 0 0.0002
Cytoplasm 0.9214 0.9917 0.9645 0.9052 0.9511 0.9711 0.6978 0.9813 0.947 0.9061 0.9001 0.9239 0.7586 0.9736 0.93 0.9433 0.9356 0.9818
Cytoplasmic Foci 0.0038 0.0007 0.0041 0.006 0.007 0.0063 0.1011 0.0009 0.0018 0.0061 0.0075 0.0031 0.0478 0.0012 0.0069 0.0179 0.0296 0.0009
Eisosomes 0.0004 0 0 0 0 0 0.0003 0 0.0001 0 0.0001 0 0.0002 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0081 0.0014 0.0058 0.0062 0.0053 0.0031 0.0049 0.0019 0.0018 0.0042 0.0078 0.0016 0.0131 0.004 0.0019 0.0009 0.0025 0.0019
Endosome 0.0073 0.0002 0.0061 0.002 0.0108 0.0018 0.0299 0.0002 0.005 0.0343 0.0029 0.0061 0.0288 0.0005 0.0056 0.0025 0.0067 0.0008
Golgi 0.0028 0.0001 0.0012 0.0029 0.0058 0.0018 0.0187 0.0001 0.0058 0.0063 0.004 0.0114 0.0081 0.0001 0.0057 0.0012 0.0024 0.0008
Lipid Particles 0.0022 0 0.0026 0.003 0.0005 0.0012 0.0241 0.0001 0.0007 0.0041 0.0078 0.002 0.0194 0.0002 0.0046 0.0123 0.005 0.0001
Mitochondria 0.0069 0 0.0008 0.0018 0.0012 0.0005 0.0062 0 0.0279 0.0033 0.0435 0.0056 0.0024 0.0001 0.0029 0.0011 0.0002 0.0073
None 0.0032 0.0004 0.001 0.0009 0.001 0.0007 0.0316 0.0059 0.0008 0.0011 0.0069 0.0075 0.023 0.0009 0.0012 0.0013 0.0019 0.0004
Nuclear Periphery 0.0041 0.0005 0.0032 0.001 0.0008 0.0006 0.0027 0.0006 0.0004 0.0151 0.0017 0.0006 0.0067 0.0006 0.001 0.0006 0.0007 0.0005
Nucleolus 0.0003 0 0.0001 0 0.0001 0 0.0002 0 0 0.0001 0 0.0002 0.0008 0 0.0001 0.0001 0 0
Nucleus 0.0038 0.0011 0.0021 0.0018 0.0028 0.0016 0.0009 0.0011 0.0006 0.0019 0.0009 0.0229 0.0057 0.001 0.0009 0.001 0.0009 0.001
Peroxisomes 0.0016 0 0.0015 0.0334 0.0006 0.0033 0.0264 0 0.0011 0.0008 0.0098 0.0027 0.0247 0 0.0044 0.0103 0.0034 0
Punctate Nuclear 0.0012 0.0001 0.0005 0.0003 0.0002 0.0002 0.0079 0.0009 0.0001 0.0023 0.0013 0.0014 0.0138 0.0002 0.0013 0.0042 0.0043 0.0001
Vacuole 0.0025 0.0005 0.002 0.0017 0.0025 0.0006 0.0012 0.0002 0.0003 0.0012 0.0006 0.0003 0.0037 0.0005 0.0006 0.0002 0.0001 0.0002
Vacuole Periphery 0.0008 0 0.0006 0.0002 0.0004 0.0001 0.0007 0 0.0003 0.0015 0.0002 0.0002 0.0009 0 0.0002 0.0001 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 183.9007 108.4087 100.0256 174.0709 137.0615 270.316 255.6409 258.4455 290.3919 242.9636
Translational Efficiency 1.1513 0.9296 1.4278 1.1795 1.1735 1.4566 1.2365 1.1787 1.2524 1.1995

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Citrate synthase, peroxisomal isozyme involved in glyoxylate cycle; catalyzes condensation of acetyl coenzyme A and oxaloacetate to form citrate; expression is controlled by Rtg1p and Rtg2p transcription factors; SCF-Ucc1 regulates level of Cit2p to maintain citrate homeostasis; oxaloacetate-dependent positive feedback loop inhibits Cit2p ubiquitination; CIT2 has a paralog, CIT1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (61%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Cit2

Cit2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cit2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available