Standard name
Description Citrate synthase, peroxisomal isozyme involved in glyoxylate cycle; catalyzes condensation of acetyl coenzyme A and oxaloacetate to form citrate; expression is controlled by Rtg1p and Rtg2p transcription factors; SCF-Ucc1 regulates level of Cit2p to maintain citrate homeostasis; oxaloacetate-dependent positive feedback loop inhibits Cit2p ubiquitination; CIT2 has a paralog, CIT1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.09 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.97 0.96 0.94 0.9 0.88 0.9 0.76 0.79 0.69 0.78 0.97 0.99 0.99 0.95 0.98 0.96 0.77 0.83 0.72 0.73 0.77 0.74
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.07 0.09 0.06 0.09 0.15 0.26 0.16 0.32 0.41 0.47 0.4 0 0 0 0.05 0 0 0.09 0.07 0.15 0 0 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 4 3 1 5 5 8
Bud 3 0 2 1 2 4 4 4 7 7 8 6 10 6 0 0 0 0 3 1 1 22 28 22
Bud Neck 2 1 0 0 0 1 2 4 0 2 0 0 0 0 0 0 0 0 2 1 1 3 3 5
Bud Site 0 0 0 0 0 0 1 0 2 1 0 1 0 0 0 0 0 0
Cell Periphery 4 0 0 0 4 2 1 2 0 1 1 3 7 4 10 3 9 0 0 0 0 0 1 3
Cytoplasm 250 198 116 153 100 181 220 258 161 222 139 236 420 569 620 250 162 101 223 174 82 185 247 340
Endoplasmic Reticulum 1 0 0 1 0 0 0 1 2 0 1 0 0 0 2 21 9 6 9 0 0 3 3 5
Endosome 0 0 0 1 1 2 0 2 0 0 0 0 0 1 1 9 1 1 4 1 1 0 0 5
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1 0 1 1 1 0 0 1 2
Mitochondria 13 14 11 10 10 31 65 46 68 115 95 120 1 0 1 14 4 2 25 15 17 9 8 26
Nucleus 0 1 0 0 0 1 0 2 1 2 4 0 1 1 0 1 1 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 0 1 3 2 9 0 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 1 0 0 1 0 1 0 1 1 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 3 0 0 0 0 2 1 3 1 2 0 4 3 0 0 0 0 1 4 1 0 11 10 8
Vac/Vac Membrane 1 0 0 0 3 2 2 4 4 6 13 1 2 4 3 2 1 3 3 3 2 3 3 14
Unique Cell Count 262 202 119 159 106 201 250 287 212 282 202 301 431 577 627 264 166 105 289 210 115 253 324 460
Labelled Cell Count 278 214 129 168 121 227 296 328 250 361 270 371 445 587 638 302 187 115 289 210 115 253 324 460


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 3.5 3.4 4.5 6.1 3.9 3.0 3.5 3.5 2.7 4.3 2.9 8.6 7.1 6.5 5.3 6.1 6.1 4.8 5.4 5.7
Std Deviation (1e-4) 0.9 0.7 1.4 1.5 2.7 1.2 1.0 1.6 1.5 1.0 1.9 1.3 5.8 4.3 3.0 2.7 2.3 1.9 2.2 2.1 1.9
Intensity Change (Log2) 0.43 0.86 0.21 -0.18 0.06 0.07 -0.32 0.36 -0.23 1.34 1.07 0.95 0.65 0.84 0.86 0.52 0.69 0.75


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cit2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available