Standard name
Description Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; ELO2 has a paralog, ELO1, that arose from the whole genome duplication; lethality of the elo2 elo3 double null mutation is functionally complemented by human ELOVL1 and weakly complemented by human ELOVL3 or ELOV7

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.10 0.08 0.06 0.05 0.08 0.08 0.06 0.09 0 0 0 0.05 0.05 0.07 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0.05 0.10 0.16 0.22 0.24 0.23 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.07 0.10 0.11 0.07 0.08 0.08 0.12 0.11 0 0 0 0.17 0.22 0.20 0 0 0 0 0 0
Endoplasmic Reticulum 0.73 0.92 0.92 0.70 0.75 0.70 0.72 0.62 0.66 0.55 0.52 0.87 0.90 0.90 0 0 0 0.92 0.94 0.92 0.89 0.85 0.83
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.19 0 0.05 0.22 0.09 0 0 0 0 0 0 0 0.07 0.05 0.53 0.37 0.44 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.09 0.08 0.08 0.22 0.25 0 0 0 0.09 0.10 0.07 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0.26 0.15 0.08 0 0.11 0.08 0.07 0.09 0.09 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0 0 0.06 0.10 0.12 0.12 0.22 0.16 0.12 0.14 0.06 0 0 0 0.05 0 0 0 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 4 18 27 23 32 43 47 26 56 6 13 13 9 5 6 5 0 2 2 8 4
Bud 0 0 0 0 0 4 1 3 10 11 12 0 0 1 0 0 1 0 0 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 12 5 5 3 11 9 17 20 32 26 32 11 32 49 36 25 20 0 0 0 1 0 1
Cytoplasm 5 8 9 12 32 41 43 47 49 57 64 7 13 11 28 23 18 0 1 1 2 2 3
Endoplasmic Reticulum 209 166 174 126 244 257 433 352 402 258 312 187 282 273 1 1 1 340 179 194 123 232 221
Endosome 0 0 0 0 0 1 0 0 1 0 3 0 0 0 1 0 0 0 1 0 1 0 0
Golgi 56 3 10 40 30 2 17 18 12 2 4 9 21 15 88 38 39 2 0 1 2 6 7
Mitochondria 0 1 0 5 14 33 56 47 50 102 151 0 0 0 15 10 6 3 0 1 0 5 2
Nucleus 1 0 0 0 0 4 2 3 1 2 0 0 0 0 1 1 2 0 0 0 0 0 0
Nuclear Periphery 13 1 50 26 26 16 63 43 43 40 55 9 14 3 3 5 2 7 1 3 0 4 2
Nucleolus 1 0 0 0 2 1 1 3 5 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 45 8 2 10 31 42 74 126 99 58 84 13 3 2 5 5 3 5 3 2 2 6 15
Unique Cell Count 288 180 189 179 325 365 599 568 605 467 601 216 315 305 167 104 88 371 190 210 139 272 268
Labelled Cell Count 342 193 254 240 417 433 739 705 751 583 774 242 378 367 187 113 98 371 190 210 139 272 268

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Endoplasmic Reticulum

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 45.5 44.3 25.3 24.9 21.0 16.4 15.3 14.9 13.7 11.9 12.0 35.5 35.9 34.2 38.9 39.3 38.6 39.5 39.4 39.2
Std Deviation (1e-4) 11.5 8.5 5.6 6.2 4.7 3.4 3.3 3.1 3.0 2.7 2.7 7.0 7.3 6.4 9.1 9.5 9.6 8.1 11.0 9.1
Intensity Change (Log2) -0.02 -0.27 -0.62 -0.72 -0.77 -0.88 -1.09 -1.07 0.49 0.51 0.44 0.62 0.64 0.61 0.64 0.64 0.63

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 3.2 2.8 2.2 1.9 2.7 2.8 1.9 3.3 0 1.2 1.3 1.6 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.5 0 0.1 0.6 1.5 1.6 1.5 1.3 3.1 4.6 5.6 5.8 5.4
Cytoplasm 0.1 1.1 1.0 0.2 0.5 1.3 1.6 1.9 0 0 -0.4 3.0 2.9 3.8
Endoplasmic Reticulum -5.4 -4.8 -5.8 -5.6 -7.8 -6.9 -9.0 -9.9 -1.8 -0.9 -0.9 -17.2 -15.2 -14.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 4.8 1.6 0 -1.6 -1.3 -2.4 0 0 -0.5 0.6 -0.2 10.0 6.9 7.9
Mitochondria 0 2.9 4.3 4.4 4.1 4.1 7.0 7.7 0 0 0 4.2 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -2.8 -5.7 -7.6 -5.5 -6.9 -7.2 -6.0 -6.1 -6.3 -7.2 -8.9 -6.5 -4.5 -4.8
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0