Standard name
Human Ortholog
Description Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; ELO2 has a paralog, ELO1, that arose from the whole genome duplication; lethality of the elo2 elo3 double null mutation is functionally complemented by human ELOVL1 and weakly complemented by human ELOVL3 or ELOV7
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.1 0.08 0.06 0.05 0.08 – 0.08 0.06 0.09 0 0 0 0.05 0.05 0.07 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0.05 0.06 0.05 0.05 0.1 0.16 0.22 0.24 0.23 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.07 0.1 0.11 0.07 0.08 – 0.08 0.12 0.11 0 0 0 0.17 0.22 0.2 0 0 0 0 0 0
Endoplasmic Reticulum 0.73 0.92 0.92 0.7 0.75 0.7 0.72 0.62 – 0.66 0.55 0.52 0.87 0.9 0.9 0 0 0 0.92 0.94 0.92 0.89 0.85 0.83
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.19 0 0.05 0.22 0.09 0 0 0 – 0 0 0 0 0.07 0.05 0.53 0.37 0.44 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.09 0.08 – 0.08 0.22 0.25 0 0 0 0.09 0.1 0.07 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0.26 0.15 0.08 0 0.11 0.08 – 0.07 0.09 0.09 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0 0 0.06 0.1 0.12 0.12 0.22 – 0.16 0.12 0.14 0.06 0 0 0 0.05 0 0 0 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 4 18 27 23 32 43 – 47 26 56 6 13 13 9 5 6 5 0 2 2 8 4
Bud 0 0 0 0 0 4 1 3 – 10 11 12 0 0 1 0 0 1 0 0 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 12 5 5 3 11 9 17 20 – 32 26 32 11 32 49 36 25 20 0 0 0 1 0 1
Cytoplasm 5 8 9 12 32 41 43 47 – 49 57 64 7 13 11 28 23 18 0 1 1 2 2 3
Endoplasmic Reticulum 209 166 174 126 244 257 433 352 – 402 258 312 187 282 273 1 1 1 340 179 194 123 232 221
Endosome 0 0 0 0 0 1 0 0 – 1 0 3 0 0 0 1 0 0 0 1 0 1 0 0
Golgi 56 3 10 40 30 2 17 18 – 12 2 4 9 21 15 88 38 39 2 0 1 2 6 7
Mitochondria 0 1 0 5 14 33 56 47 – 50 102 151 0 0 0 15 10 6 3 0 1 0 5 2
Nucleus 1 0 0 0 0 4 2 3 – 1 2 0 0 0 0 1 1 2 0 0 0 0 0 0
Nuclear Periphery 13 1 50 26 26 16 63 43 – 43 40 55 9 14 3 3 5 2 7 1 3 0 4 2
Nucleolus 1 0 0 0 2 1 1 3 – 5 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 45 8 2 10 31 42 74 126 – 99 58 84 13 3 2 5 5 3 5 3 2 2 6 15
Unique Cell Count 288 180 189 179 325 365 599 568 605 467 601 216 315 305 167 104 88 371 190 210 139 272 268
Labelled Cell Count 342 193 254 240 417 433 739 705 751 583 774 242 378 367 187 113 98 371 190 210 139 272 268


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 45.5 44.3 25.3 24.9 21.0 16.4 15.3 14.9 – 13.7 11.9 12.0 35.5 35.9 34.2 38.9 39.3 38.6 39.5 39.4 39.2
Std Deviation (1e-4) 11.5 8.5 5.6 6.2 4.7 3.4 3.3 3.1 – 3.0 2.7 2.7 7.0 7.3 6.4 9.1 9.5 9.6 8.1 11.0 9.1
Intensity Change (Log2) – – – -0.02 -0.27 -0.62 -0.72 -0.77 – -0.88 -1.09 -1.07 0.49 0.51 0.44 0.62 0.64 0.61 0.64 0.64 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 3.2 2.8 2.2 1.9 2.7 – 2.8 1.9 3.3 0 1.2 1.3 1.6 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.5 0 0.1 0.6 – 1.5 1.6 1.5 1.3 3.1 4.6 5.6 5.8 5.4
Cytoplasm 0.8 2.1 2.5 1.2 1.6 – 1.5 2.9 2.4 -0.8 -0.3 -0.6 3.7 4.6 4.1
Endoplasmic Reticulum -5.4 -4.8 -5.8 -5.6 -7.8 – -6.9 -9.0 -9.9 -1.8 -0.9 -0.9 -17.2 -15.2 -14.6
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 4.8 1.6 0 -1.6 -1.3 – -2.4 0 0 -0.5 0.6 -0.2 10.0 6.9 7.9
Mitochondria 0 2.9 4.3 4.4 4.1 – 4.1 7.0 7.7 0 0 0 4.2 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery -2.8 -5.7 -7.6 -5.5 -6.9 – -7.2 -6.0 -6.1 -6.3 -7.2 -8.9 -6.5 -4.5 -4.8
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 2.4 3.8 4.3 4.6 6.7 – 5.5 4.6 5.0 2.6 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 29.5099 31.0632 32.4347 31.3991 27.1744 32.0477 32.2277 35.2529 33.7784 30.3407 31.1972 34.3377 35.9884 32.9853 36.498 34.6155 32.2418 33.3491
Actin 0.0122 0.001 0.0014 0.0001 0.0182 0.0003 0.0148 0.0003 0.0155 0.0018 0.0036 0.0001 0.0019 0.0002 0.0001 0 0.0298 0.0017
Bud 0.0003 0 0.0002 0 0.0003 0 0.0003 0 0.0002 0.0001 0 0 0.0003 0 0 0 0.0001 0
Bud Neck 0.0004 0.0003 0.0002 0.0003 0.0006 0.0002 0.0003 0.0002 0.0007 0.0001 0.0002 0.0002 0.0002 0.0004 0.0002 0.0002 0.0005 0.0007
Bud Periphery 0.001 0.0001 0.0003 0 0.0003 0.0001 0.0005 0.0001 0.0002 0.0004 0.0001 0.0001 0.0004 0.0002 0 0 0.0004 0
Bud Site 0.0011 0.0004 0.0002 0 0.0006 0 0.0009 0 0.0015 0.0001 0.0001 0 0.0005 0.0001 0 0 0.0001 0
Cell Periphery 0.0033 0.0081 0.0054 0.0024 0.0011 0.0009 0.0055 0.0054 0.003 0.0042 0.004 0.0009 0.0045 0.0096 0.0042 0.0014 0.0068 0.0007
Cytoplasm 0.0302 0.0663 0.0161 0.0062 0.0017 0.0019 0.0025 0.0032 0.0036 0.002 0.001 0.0005 0.0042 0.0149 0.0052 0.0007 0.0014 0.0008
Cytoplasmic Foci 0.0236 0.0005 0.0109 0.0001 0.0398 0.0021 0.013 0.0002 0.0094 0.0016 0.0248 0 0.0015 0.0003 0 0 0.0008 0.0018
Eisosomes 0.0004 0.0002 0.0006 0.0001 0.0001 0 0.0007 0 0.0002 0.0007 0.0002 0 0.0005 0.0001 0 0.0001 0.0006 0
Endoplasmic Reticulum 0.7099 0.8299 0.8941 0.9079 0.809 0.8855 0.6932 0.9335 0.9012 0.8158 0.8124 0.8501 0.7807 0.9041 0.9539 0.9832 0.867 0.8809
Endosome 0.0152 0.0007 0.0097 0.0022 0.019 0.0031 0.0143 0.0007 0.0024 0.0106 0.0045 0.0005 0.0041 0.001 0.0002 0.0003 0.002 0.0007
Golgi 0.0036 0.0003 0.0018 0.0003 0.0338 0.0012 0.0117 0.0005 0.0018 0.0105 0.0039 0.0004 0.001 0.0013 0.0002 0.0004 0.0017 0.0003
Lipid Particles 0.0058 0.0023 0.0103 0.0001 0.0154 0.01 0.0147 0.007 0.0056 0.0174 0.0346 0.0006 0.0036 0.0139 0.0005 0.0004 0.004 0.0006
Mitochondria 0.004 0.0003 0.0027 0.0001 0.01 0.0081 0.021 0.0001 0.0058 0.0389 0.0072 0.0001 0.003 0.0001 0 0 0.0084 0.0001
None 0.0062 0.0003 0.0015 0 0.0002 0.0005 0.0022 0 0.0091 0.0006 0.0005 0 0.0011 0.0001 0 0 0.0024 0.0035
Nuclear Periphery 0.1448 0.0626 0.0296 0.0687 0.0391 0.0747 0.1668 0.0427 0.031 0.0769 0.0531 0.1382 0.1709 0.0405 0.0305 0.0106 0.0479 0.1012
Nucleolus 0.001 0.0002 0.0003 0 0 0.0007 0.0007 0 0.0003 0.0002 0.0004 0 0.0006 0.0001 0 0 0.0024 0
Nucleus 0.0113 0.0127 0.0026 0.0084 0.0008 0.0031 0.0074 0.0007 0.0014 0.001 0.0011 0.0009 0.0071 0.0028 0.0012 0.0002 0.0027 0.0018
Peroxisomes 0.0029 0 0.0016 0 0.0028 0.0004 0.0047 0 0.0018 0.0003 0.0306 0 0.0001 0 0 0 0.0013 0.0006
Punctate Nuclear 0.0033 0.0006 0.0008 0 0.0006 0.0002 0.009 0.0001 0.001 0.0001 0.0008 0 0.0011 0.0001 0 0 0.0009 0.0006
Vacuole 0.0103 0.0124 0.0077 0.0022 0.0049 0.0046 0.0065 0.004 0.0028 0.0079 0.0071 0.0035 0.0069 0.0092 0.003 0.002 0.0117 0.0017
Vacuole Periphery 0.0092 0.0007 0.0021 0.0007 0.0016 0.0023 0.0093 0.0012 0.0016 0.0086 0.0097 0.004 0.006 0.001 0.0007 0.0004 0.0069 0.0022

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 172.2748 132.2727 112.1737 154.204 168.5491 100.35 136.3678 119.9248 88.0099 176.4932
Translational Efficiency 1.2463 1.6145 1.5533 0.8056 1.1085 2.0816 1.4205 1.1684 1.5095 1.1662

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; ELO2 has a paralog, ELO1, that arose from the whole genome duplication; lethality of the elo2 elo3 double null mutation is functionally complemented by human ELOVL1 and weakly complemented by human ELOVL3 or ELOV7
Localization
Cell Percentages ER (100%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Elo2

Elo2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Elo2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available