Standard name
Human Ortholog
Description Putative ribokinase

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.96 0.95 0.94 0.91 0.89 0.82 0.78 0.75 0.77 0.8 0.91 0.93 0.98 0.95 1.0 0.99 0.91 0.92 0.83 0.81 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.11 0.19 0.14 0.2 0.11 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.06 0.06 0.07 0.08 0.09 0.07 0.12 0.05 0.12 0.06 0.06 0 0.05 0 0 0 0 0.08 0.11 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 1 0 1 1 1 2 4 8 4 0 0 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cell Periphery 1 1 4 3 7 4 0 2 2 1 2 0 2 1 0 0 0 0 0 0 0 0
Cytoplasm 258 96 182 220 250 248 168 188 184 133 183 113 177 198 18 17 251 39 118 92 115 211
Endoplasmic Reticulum 0 2 0 2 1 0 2 3 5 0 5 1 1 2 1 0 0 0 1 0 1 0
Endosome 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Mitochondria 1 0 0 0 8 24 23 47 33 34 25 1 0 1 0 0 1 0 0 2 0 3
Nucleus 0 1 2 4 4 4 3 7 7 3 7 3 4 3 0 0 0 0 1 3 3 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 1 0 0 0 1 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 11 14 20 21 19 18 30 8 27 7 11 3 1 0 0 1 3 9 15 15
Unique Cell Count 259 100 192 234 274 278 204 242 244 172 230 124 190 203 19 17 255 44 129 112 142 243
Labelled Cell Count 260 101 200 243 291 305 217 268 266 188 253 126 196 208 20 17 255 44 129 112 142 243


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 7.7 8.6 8.6 7.7 7.4 7.2 6.5 6.3 6.5 6.8 8.4 9.6 10.6 16.0 16.1 7.8 8.9 10.5
Std Deviation (1e-4) 0.9 0.9 2.6 1.6 1.5 1.6 1.4 1.4 1.1 1.7 1.3 1.6 1.8 1.9 4.0 4.7 1.2 1.9 2.2
Intensity Change (Log2) 0.16 0.16 0.01 -0.05 -0.09 -0.25 -0.29 -0.25 -0.17 0.12 0.31 0.47 1.06 1.07 0.03 0.2 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 -0.7 -1.5 -2.0 -3.3 -4.1 -4.5 -4.1 -3.8 -1.4 -1.0 0.7 -0.3 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.0 3.5 4.7 3.9 4.8 3.4 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.8 2.8 3.2 2.8 3.7 0 3.6 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin
Bud
Bud Neck
Bud Periphery
Bud Site
Cell Periphery
Cytoplasm
Cytoplasmic Foci
Eisosomes
Endoplasmic Reticulum
Endosome
Golgi
Lipid Particles
Mitochondria
None
Nuclear Periphery
Nucleolus
Nucleus
Peroxisomes
Punctate Nuclear
Vacuole
Vacuole Periphery

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.5746 45.2827 29.2189 29.0864 24.2719 28.6154 30.1088 30.5661 35.9432 28.1603
Translational Efficiency 2.1298 1.0392 1.2488 1.7061 1.373 1.7449 1.7254 1.369 1.3962 1.4418

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1883 434 487 1456 2319 1 493 1106 4202 435 980 2562

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 942.20 1080.66 1266.89 1283.48 918.18 1033.51 1285.07 1212.04 928.94 1080.55 1276.04 1252.64
Standard Deviation 116.19 141.12 164.26 194.67 119.60 158.41 199.73 118.68 140.97 161.60 200.03
Intensity Change Log 2 0.197807 0.427186 0.445956 0.170703 0.484998 0.400588 0.184494 0.456008 0.423743

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000412 0.001057 0.001730 0.001260 0.000116 0.006323 0.000311 0.002587 0.000249 0.001069 0.001016 0.001833
Bud Neck 0.001795 0.003051 0.003974 0.002450 0.001725 0.002179 0.001779 0.006842 0.001757 0.003049 0.002870 0.004346
Bud Site 0.005210 0.012995 0.018359 0.015738 0.008991 0.012971 0.004499 0.046248 0.007297 0.012995 0.011387 0.028909
Cell Periphery 0.000065 0.000104 0.000161 0.000150 0.000090 0.000678 0.000121 0.000448 0.000079 0.000106 0.000141 0.000279
Cytoplasm 0.765748* 0.744737* 0.532172* 0.611251* 0.787583* 0.179242* 0.635337* 0.520402* 0.777798* 0.743437* 0.584070* 0.572032*
Cytoplasmic Foci 0.084401 0.100847 0.013808 0.023703 0.060948 0.042739 0.015416 0.037198 0.071458 0.100713 0.014617 0.029529
Eisosomes 0.000020 0.000024 0.000017 0.000018 0.000016 0.000150 0.000025 0.000029 0.000018 0.000025 0.000021 0.000023
Endoplasmic Reticulum 0.000770 0.001294 0.002040 0.002044 0.000891 0.014341 0.002093 0.003706 0.000837 0.001324 0.002067 0.002761
Endosome 0.003528 0.006160 0.001810 0.010987 0.001884 0.039129 0.002618 0.031185 0.002621 0.006236 0.002216 0.019707
Golgi 0.001121 0.002822 0.000223 0.011981 0.000651 0.005258 0.000129 0.021374 0.000861 0.002828 0.000176 0.016036
Lipid Particles 0.000993 0.001046 0.001015 0.001343 0.000644 0.026625 0.001118 0.001282 0.000801 0.001105 0.001067 0.001316
Mitochondria 0.001188 0.002695 0.000350 0.001731 0.000307 0.000381 0.000172 0.017032 0.000702 0.002689 0.000260 0.008336
Mitotic Spindle 0.005025 0.003260 0.007089 0.051170 0.001056 0.000626 0.002501 0.119295* 0.002835 0.003254 0.004781 0.080579
None 0.004768 0.004062 0.007102 0.004559 0.007705 0.001816 0.006913 0.006042 0.006388 0.004057 0.007007 0.005199
Nuclear Periphery 0.000149 0.000124 0.000987 0.001044 0.000125 0.000604 0.000939 0.000607 0.000136 0.000125 0.000963 0.000855
Nuclear Periphery Foci 0.001864 0.004312 0.003689 0.004112 0.001218 0.464407* 0.007805 0.004135 0.001508 0.005369 0.005760 0.004122
Nucleolus 0.000335 0.000274 0.000169 0.000119 0.000288 0.010072 0.000162 0.000743 0.000309 0.000296 0.000166 0.000388
Nucleus 0.077467 0.048345 0.336056* 0.142256* 0.091793 0.048914 0.176214* 0.046263 0.085374 0.048346 0.255646* 0.100816
Peroxisomes 0.000792 0.002042 0.001493 0.000713 0.000616 0.002036 0.000148 0.007811 0.000695 0.002042 0.000816 0.003777
Vacuole 0.043709 0.059728 0.067603 0.098452 0.032985 0.137322 0.141577 0.115174 0.037791 0.059906 0.104817 0.105671
Vacuole Periphery 0.000639 0.001022 0.000154 0.014921 0.000366 0.004186 0.000121 0.011596 0.000488 0.001029 0.000137 0.013486

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.14 -1.79 -0.97 0.74 1.45 NaN NaN NaN NaN NaN -1.65 -2.67 -3.09 0.76 0.78
Bud Neck -0.76 -5.08 -2.09 0.23 3.57 NaN NaN NaN NaN NaN -0.81 -5.50 -4.33 0.03 1.98
Bud Site -3.40 -2.34 -2.82 2.11 1.26 NaN NaN NaN NaN NaN -2.48 -0.87 -3.21 0.84 -1.53
Cell Periphery -3.37 -11.08 -7.69 -1.95 4.82 NaN NaN NaN NaN NaN -1.97 -8.94 -2.38 -1.02 1.98
Cytoplasm 2.55 26.09 29.13 14.97 -5.75 NaN NaN NaN NaN NaN 3.80 34.11 42.56 16.39 -3.43
Cytoplasmic Foci -2.47 23.32 24.91 13.68 0.47 NaN NaN NaN NaN NaN -4.50 30.52 31.30 13.42 -0.55
Eisosomes -2.21 -3.11 0.06 2.23 3.14 NaN NaN NaN NaN NaN -2.92 -6.21 -1.33 2.43 6.82
Endoplasmic Reticulum -2.40 -7.85 -1.70 1.44 6.79 NaN NaN NaN NaN NaN -2.11 -9.10 -0.78 1.33 6.95
Endosome -2.58 3.43 2.85 3.90 0.36 NaN NaN NaN NaN NaN -3.73 0.72 -2.45 2.15 -2.77
Golgi -1.59 3.83 -1.02 0.81 -2.67 NaN NaN NaN NaN NaN -1.92 5.65 -3.16 0.02 -4.48
Lipid Particles -0.25 -4.38 -5.11 -3.30 -0.73 NaN NaN NaN NaN NaN -1.37 -9.00 -8.41 -2.17 0.50
Mitochondria -0.67 1.26 1.29 1.11 0.28 NaN NaN NaN NaN NaN -0.96 1.37 -1.55 0.47 -2.44
Mitotic Spindle 1.07 -1.59 -4.58 -4.96 -2.32 NaN NaN NaN NaN NaN 0.03 -3.08 -10.36 -8.52 -7.78
None 0.98 -1.89 -0.62 -1.34 1.58 NaN NaN NaN NaN NaN 2.59 0.73 3.01 -1.21 2.36
Nuclear Periphery 0.58 -15.40 -13.19 -13.95 5.60 NaN NaN NaN NaN NaN -0.06 -23.08 -20.44 -20.41 7.62
Nuclear Periphery Foci -5.13 -6.79 -4.48 2.75 3.59 NaN NaN NaN NaN NaN -3.51 -12.53 -6.69 2.33 7.98
Nucleolus 0.98 1.98 3.33 1.23 3.88 NaN NaN NaN NaN NaN 0.40 2.88 0.78 0.14 -0.93
Nucleus 7.20 -25.57 -19.27 -22.05 16.73 NaN NaN NaN NaN NaN 10.05 -27.14 -13.97 -18.46 20.18
Peroxisomes -1.67 0.34 3.43 2.63 1.56 NaN NaN NaN NaN NaN -1.86 1.45 -0.22 1.74 -1.36
Vacuole -4.44 -10.76 -30.09 -20.34 -10.35 NaN NaN NaN NaN NaN -5.72 -19.89 -43.85 -26.01 -10.13
Vacuole Periphery -0.73 1.49 -1.70 -1.34 -2.37 NaN NaN NaN NaN NaN -1.41 2.33 -2.83 -1.81 -3.54