Standard name | |
---|---|
Human Ortholog | |
Description | Putative ribokinase |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | – | 0 | 1 | 0 | 1 | 1 | 1 | 2 | 4 | 8 | 4 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | – | 1 | 4 | 3 | 7 | 4 | 0 | 2 | 2 | 1 | 2 | 0 | 2 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 258 | – | 96 | 182 | 220 | 250 | 248 | 168 | 188 | 184 | 133 | 183 | 113 | 177 | 198 | 18 | – | 17 | 251 | 39 | 118 | 92 | 115 | 211 |
Endoplasmic Reticulum | 0 | – | 2 | 0 | 2 | 1 | 0 | 2 | 3 | 5 | 0 | 5 | 1 | 1 | 2 | 1 | – | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Endosome | 0 | – | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Golgi | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | – | 0 | 0 | 0 | 8 | 24 | 23 | 47 | 33 | 34 | 25 | 1 | 0 | 1 | 0 | – | 0 | 1 | 0 | 0 | 2 | 0 | 3 |
Nucleus | 0 | – | 1 | 2 | 4 | 4 | 4 | 3 | 7 | 7 | 3 | 7 | 3 | 4 | 3 | 0 | – | 0 | 0 | 0 | 1 | 3 | 3 | 2 |
Nuclear Periphery | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | – | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
SpindlePole | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | – | 0 | 11 | 14 | 20 | 21 | 19 | 18 | 30 | 8 | 27 | 7 | 11 | 3 | 1 | – | 0 | 0 | 1 | 3 | 9 | 15 | 15 |
Unique Cell Count | 259 | 100 | 192 | 234 | 274 | 278 | 204 | 242 | 244 | 172 | 230 | 124 | 190 | 203 | 19 | 17 | 255 | 44 | 129 | 112 | 142 | 243 | ||
Labelled Cell Count | 260 | 101 | 200 | 243 | 291 | 305 | 217 | 268 | 266 | 188 | 253 | 126 | 196 | 208 | 20 | 17 | 255 | 44 | 129 | 112 | 142 | 243 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.2 | – | 7.7 | 8.6 | 8.6 | 7.7 | 7.4 | 7.2 | 6.5 | 6.3 | 6.5 | 6.8 | 8.4 | 9.6 | 10.6 | 16.0 | – | 16.1 | 7.8 | 8.9 | 10.5 |
Std Deviation (1e-4) | 0.9 | – | 0.9 | 2.6 | 1.6 | 1.5 | 1.6 | 1.4 | 1.4 | 1.1 | 1.7 | 1.3 | 1.6 | 1.8 | 1.9 | 4.0 | – | 4.7 | 1.2 | 1.9 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.16 | 0.16 | 0.01 | -0.05 | -0.09 | -0.25 | -0.29 | -0.25 | -0.17 | 0.12 | 0.31 | 0.47 | 1.06 | – | 1.07 | 0.03 | 0.2 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Cytoplasm | -0.5 | -0.7 | -1.5 | -2.0 | -3.3 | -4.1 | -4.5 | -4.1 | -3.8 | -1.4 | -1.0 | 0.7 | -0.3 | – | 0.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Mitochondria | 0 | 0 | 0 | 3.0 | 3.5 | 4.7 | 3.9 | 4.8 | 3.4 | 0 | 0 | 0 | 0 | – | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Vacuole | 0 | 0 | 2.8 | 2.8 | 3.2 | 2.8 | 3.7 | 0 | 3.6 | 0 | 0 | 0 | 0 | – | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasmic Foci | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Eisosomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Lipid Particles | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
None | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Punctate Nuclear | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.5746 | 45.2827 | 29.2189 | 29.0864 | 24.2719 | 28.6154 | 30.1088 | 30.5661 | 35.9432 | 28.1603 |
Translational Efficiency | 2.1298 | 1.0392 | 1.2488 | 1.7061 | 1.373 | 1.7449 | 1.7254 | 1.369 | 1.3962 | 1.4418 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | – |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1883 | 434 | 487 | 1456 | 2319 | 1 | 493 | 1106 | 4202 | 435 | 980 | 2562 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 942.20 | 1080.66 | 1266.89 | 1283.48 | 918.18 | 1033.51 | 1285.07 | 1212.04 | 928.94 | 1080.55 | 1276.04 | 1252.64 |
Standard Deviation | 116.19 | 141.12 | 164.26 | 194.67 | 119.60 | — | 158.41 | 199.73 | 118.68 | 140.97 | 161.60 | 200.03 |
Intensity Change Log 2 | — | 0.197807 | 0.427186 | 0.445956 | — | 0.170703 | 0.484998 | 0.400588 | — | 0.184494 | 0.456008 | 0.423743 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000412 | 0.001057 | 0.001730 | 0.001260 | 0.000116 | 0.006323 | 0.000311 | 0.002587 | 0.000249 | 0.001069 | 0.001016 | 0.001833 |
Bud Neck | 0.001795 | 0.003051 | 0.003974 | 0.002450 | 0.001725 | 0.002179 | 0.001779 | 0.006842 | 0.001757 | 0.003049 | 0.002870 | 0.004346 |
Bud Site | 0.005210 | 0.012995 | 0.018359 | 0.015738 | 0.008991 | 0.012971 | 0.004499 | 0.046248 | 0.007297 | 0.012995 | 0.011387 | 0.028909 |
Cell Periphery | 0.000065 | 0.000104 | 0.000161 | 0.000150 | 0.000090 | 0.000678 | 0.000121 | 0.000448 | 0.000079 | 0.000106 | 0.000141 | 0.000279 |
Cytoplasm | 0.765748* | 0.744737* | 0.532172* | 0.611251* | 0.787583* | 0.179242* | 0.635337* | 0.520402* | 0.777798* | 0.743437* | 0.584070* | 0.572032* |
Cytoplasmic Foci | 0.084401 | 0.100847 | 0.013808 | 0.023703 | 0.060948 | 0.042739 | 0.015416 | 0.037198 | 0.071458 | 0.100713 | 0.014617 | 0.029529 |
Eisosomes | 0.000020 | 0.000024 | 0.000017 | 0.000018 | 0.000016 | 0.000150 | 0.000025 | 0.000029 | 0.000018 | 0.000025 | 0.000021 | 0.000023 |
Endoplasmic Reticulum | 0.000770 | 0.001294 | 0.002040 | 0.002044 | 0.000891 | 0.014341 | 0.002093 | 0.003706 | 0.000837 | 0.001324 | 0.002067 | 0.002761 |
Endosome | 0.003528 | 0.006160 | 0.001810 | 0.010987 | 0.001884 | 0.039129 | 0.002618 | 0.031185 | 0.002621 | 0.006236 | 0.002216 | 0.019707 |
Golgi | 0.001121 | 0.002822 | 0.000223 | 0.011981 | 0.000651 | 0.005258 | 0.000129 | 0.021374 | 0.000861 | 0.002828 | 0.000176 | 0.016036 |
Lipid Particles | 0.000993 | 0.001046 | 0.001015 | 0.001343 | 0.000644 | 0.026625 | 0.001118 | 0.001282 | 0.000801 | 0.001105 | 0.001067 | 0.001316 |
Mitochondria | 0.001188 | 0.002695 | 0.000350 | 0.001731 | 0.000307 | 0.000381 | 0.000172 | 0.017032 | 0.000702 | 0.002689 | 0.000260 | 0.008336 |
Mitotic Spindle | 0.005025 | 0.003260 | 0.007089 | 0.051170 | 0.001056 | 0.000626 | 0.002501 | 0.119295* | 0.002835 | 0.003254 | 0.004781 | 0.080579 |
None | 0.004768 | 0.004062 | 0.007102 | 0.004559 | 0.007705 | 0.001816 | 0.006913 | 0.006042 | 0.006388 | 0.004057 | 0.007007 | 0.005199 |
Nuclear Periphery | 0.000149 | 0.000124 | 0.000987 | 0.001044 | 0.000125 | 0.000604 | 0.000939 | 0.000607 | 0.000136 | 0.000125 | 0.000963 | 0.000855 |
Nuclear Periphery Foci | 0.001864 | 0.004312 | 0.003689 | 0.004112 | 0.001218 | 0.464407* | 0.007805 | 0.004135 | 0.001508 | 0.005369 | 0.005760 | 0.004122 |
Nucleolus | 0.000335 | 0.000274 | 0.000169 | 0.000119 | 0.000288 | 0.010072 | 0.000162 | 0.000743 | 0.000309 | 0.000296 | 0.000166 | 0.000388 |
Nucleus | 0.077467 | 0.048345 | 0.336056* | 0.142256* | 0.091793 | 0.048914 | 0.176214* | 0.046263 | 0.085374 | 0.048346 | 0.255646* | 0.100816 |
Peroxisomes | 0.000792 | 0.002042 | 0.001493 | 0.000713 | 0.000616 | 0.002036 | 0.000148 | 0.007811 | 0.000695 | 0.002042 | 0.000816 | 0.003777 |
Vacuole | 0.043709 | 0.059728 | 0.067603 | 0.098452 | 0.032985 | 0.137322 | 0.141577 | 0.115174 | 0.037791 | 0.059906 | 0.104817 | 0.105671 |
Vacuole Periphery | 0.000639 | 0.001022 | 0.000154 | 0.014921 | 0.000366 | 0.004186 | 0.000121 | 0.011596 | 0.000488 | 0.001029 | 0.000137 | 0.013486 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.14 | -1.79 | -0.97 | 0.74 | 1.45 | NaN | NaN | NaN | NaN | NaN | -1.65 | -2.67 | -3.09 | 0.76 | 0.78 |
Bud Neck | -0.76 | -5.08 | -2.09 | 0.23 | 3.57 | NaN | NaN | NaN | NaN | NaN | -0.81 | -5.50 | -4.33 | 0.03 | 1.98 |
Bud Site | -3.40 | -2.34 | -2.82 | 2.11 | 1.26 | NaN | NaN | NaN | NaN | NaN | -2.48 | -0.87 | -3.21 | 0.84 | -1.53 |
Cell Periphery | -3.37 | -11.08 | -7.69 | -1.95 | 4.82 | NaN | NaN | NaN | NaN | NaN | -1.97 | -8.94 | -2.38 | -1.02 | 1.98 |
Cytoplasm | 2.55 | 26.09 | 29.13 | 14.97 | -5.75 | NaN | NaN | NaN | NaN | NaN | 3.80 | 34.11 | 42.56 | 16.39 | -3.43 |
Cytoplasmic Foci | -2.47 | 23.32 | 24.91 | 13.68 | 0.47 | NaN | NaN | NaN | NaN | NaN | -4.50 | 30.52 | 31.30 | 13.42 | -0.55 |
Eisosomes | -2.21 | -3.11 | 0.06 | 2.23 | 3.14 | NaN | NaN | NaN | NaN | NaN | -2.92 | -6.21 | -1.33 | 2.43 | 6.82 |
Endoplasmic Reticulum | -2.40 | -7.85 | -1.70 | 1.44 | 6.79 | NaN | NaN | NaN | NaN | NaN | -2.11 | -9.10 | -0.78 | 1.33 | 6.95 |
Endosome | -2.58 | 3.43 | 2.85 | 3.90 | 0.36 | NaN | NaN | NaN | NaN | NaN | -3.73 | 0.72 | -2.45 | 2.15 | -2.77 |
Golgi | -1.59 | 3.83 | -1.02 | 0.81 | -2.67 | NaN | NaN | NaN | NaN | NaN | -1.92 | 5.65 | -3.16 | 0.02 | -4.48 |
Lipid Particles | -0.25 | -4.38 | -5.11 | -3.30 | -0.73 | NaN | NaN | NaN | NaN | NaN | -1.37 | -9.00 | -8.41 | -2.17 | 0.50 |
Mitochondria | -0.67 | 1.26 | 1.29 | 1.11 | 0.28 | NaN | NaN | NaN | NaN | NaN | -0.96 | 1.37 | -1.55 | 0.47 | -2.44 |
Mitotic Spindle | 1.07 | -1.59 | -4.58 | -4.96 | -2.32 | NaN | NaN | NaN | NaN | NaN | 0.03 | -3.08 | -10.36 | -8.52 | -7.78 |
None | 0.98 | -1.89 | -0.62 | -1.34 | 1.58 | NaN | NaN | NaN | NaN | NaN | 2.59 | 0.73 | 3.01 | -1.21 | 2.36 |
Nuclear Periphery | 0.58 | -15.40 | -13.19 | -13.95 | 5.60 | NaN | NaN | NaN | NaN | NaN | -0.06 | -23.08 | -20.44 | -20.41 | 7.62 |
Nuclear Periphery Foci | -5.13 | -6.79 | -4.48 | 2.75 | 3.59 | NaN | NaN | NaN | NaN | NaN | -3.51 | -12.53 | -6.69 | 2.33 | 7.98 |
Nucleolus | 0.98 | 1.98 | 3.33 | 1.23 | 3.88 | NaN | NaN | NaN | NaN | NaN | 0.40 | 2.88 | 0.78 | 0.14 | -0.93 |
Nucleus | 7.20 | -25.57 | -19.27 | -22.05 | 16.73 | NaN | NaN | NaN | NaN | NaN | 10.05 | -27.14 | -13.97 | -18.46 | 20.18 |
Peroxisomes | -1.67 | 0.34 | 3.43 | 2.63 | 1.56 | NaN | NaN | NaN | NaN | NaN | -1.86 | 1.45 | -0.22 | 1.74 | -1.36 |
Vacuole | -4.44 | -10.76 | -30.09 | -20.34 | -10.35 | NaN | NaN | NaN | NaN | NaN | -5.72 | -19.89 | -43.85 | -26.01 | -10.13 |
Vacuole Periphery | -0.73 | 1.49 | -1.70 | -1.34 | -2.37 | NaN | NaN | NaN | NaN | NaN | -1.41 | 2.33 | -2.83 | -1.81 | -3.54 |