| Standard name | |
|---|---|
| Description | Low-affinity inorganic phosphate (Pi) transporter; acts upstream of Pho81p in regulation of the PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | 4 | 4 | 21 | 4 | 12 | 14 |
| Bud | 0 | 2 | 3 | 4 | 3 | 7 | 8 | 6 | – | 18 | 20 | 28 | 0 | 0 | 0 | – | – | – | 2 | 0 | 5 | 2 | 22 | 12 |
| Bud Neck | 0 | 1 | 4 | 0 | 1 | 0 | 1 | 2 | – | 3 | 1 | 1 | 1 | 2 | 2 | – | – | – | 1 | 3 | 5 | 1 | 8 | 8 |
| Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 4 | 2 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
| Cell Periphery | 3 | 3 | 1 | 2 | 0 | 4 | 7 | 3 | – | 4 | 1 | 3 | 4 | 11 | 3 | – | – | – | 1 | 3 | 8 | 4 | 12 | 9 |
| Cytoplasm | 194 | 259 | 423 | 272 | 464 | 560 | 748 | 715 | – | 587 | 314 | 485 | 262 | 264 | 380 | – | – | – | 127 | 223 | 217 | 112 | 357 | 328 |
| Endoplasmic Reticulum | 0 | 6 | 2 | 5 | 1 | 3 | 3 | 0 | – | 0 | 1 | 2 | 8 | 5 | 2 | – | – | – | 23 | 12 | 48 | 12 | 14 | 28 |
| Endosome | 0 | 0 | 1 | 1 | 13 | 7 | 12 | 9 | – | 0 | 1 | 1 | 0 | 0 | 0 | – | – | – | 1 | 2 | 7 | 0 | 8 | 5 |
| Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | 0 | 1 | 1 | 0 | 0 | 0 |
| Mitochondria | 1 | 8 | 6 | 12 | 29 | 167 | 276 | 250 | – | 347 | 270 | 430 | 0 | 0 | 0 | – | – | – | 23 | 21 | 96 | 32 | 64 | 56 |
| Nucleus | 0 | 0 | 3 | 2 | 1 | 0 | 5 | 6 | – | 5 | 1 | 3 | 0 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 |
| Nuclear Periphery | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | – | 2 | 1 | 2 | 0 | 0 | 0 | – | – | – | 0 | 0 | 2 | 0 | 1 | 0 |
| Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 |
| SpindlePole | 1 | 1 | 1 | 2 | 5 | 3 | 8 | 7 | – | 12 | 12 | 14 | 0 | 0 | 0 | – | – | – | 1 | 2 | 4 | 0 | 7 | 7 |
| Vac/Vac Membrane | 2 | 0 | 0 | 1 | 17 | 19 | 33 | 18 | – | 17 | 6 | 12 | 0 | 0 | 0 | – | – | – | 1 | 3 | 6 | 4 | 20 | 18 |
| Unique Cell Count | 196 | 274 | 436 | 294 | 506 | 677 | 949 | 904 | 811 | 480 | 780 | 271 | 270 | 382 | 194 | 286 | 442 | 182 | 545 | 505 | ||||
| Labelled Cell Count | 201 | 280 | 445 | 306 | 535 | 770 | 1101 | 1022 | 998 | 632 | 984 | 277 | 282 | 388 | 194 | 286 | 442 | 182 | 545 | 505 | ||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 4.6 | 3.8 | 3.9 | 4.3 | 4.3 | 3.5 | 3.3 | 3.5 | – | 3.0 | 3.1 | 3.0 | 5.2 | 5.1 | 5.3 | – | 4.1 | 4.9 | 5.5 | 5.5 | 5.7 |
| Std Deviation (1e-4) | 2.1 | 0.6 | 1.2 | 1.9 | 1.8 | 1.3 | 1.3 | 1.1 | – | 1.0 | 1.3 | 0.9 | 0.8 | 0.8 | 1.0 | – | 1.2 | 1.5 | 1.5 | 1.7 | 1.8 |
| Intensity Change (Log2) | – | – | – | 0.14 | 0.14 | -0.17 | -0.24 | -0.13 | – | -0.36 | -0.32 | -0.36 | 0.44 | 0.4 | 0.46 | – | 0.07 | 0.33 | 0.5 | 0.51 | 0.56 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
