Standard name
Human Ortholog
Description Low-affinity inorganic phosphate (Pi) transporter; acts upstream of Pho81p in regulation of the PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0.05 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.99 0.95 0.97 0.93 0.92 0.83 0.79 0.79 – 0.72 0.65 0.62 0.97 0.98 0.99 – – – 0.66 0.78 0.49 0.62 0.66 0.65
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0.12 0.05 0.11 0.07 0 0.06
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.06 0.25 0.29 0.28 – 0.43 0.56 0.55 0 0 0 – – – 0.12 0.08 0.22 0.18 0.12 0.11
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 3 0 0 0 0 – 0 0 0 1 0 0 – – – 4 4 21 4 12 14
Bud 0 2 3 4 3 7 8 6 – 18 20 28 0 0 0 – – – 2 0 5 2 22 12
Bud Neck 0 1 4 0 1 0 1 2 – 3 1 1 1 2 2 – – – 1 3 5 1 8 8
Bud Site 0 0 0 0 0 0 0 1 – 1 4 2 0 0 0 – – – – – – – – –
Cell Periphery 3 3 1 2 0 4 7 3 – 4 1 3 4 11 3 – – – 1 3 8 4 12 9
Cytoplasm 194 259 423 272 464 560 748 715 – 587 314 485 262 264 380 – – – 127 223 217 112 357 328
Endoplasmic Reticulum 0 6 2 5 1 3 3 0 – 0 1 2 8 5 2 – – – 23 12 48 12 14 28
Endosome 0 0 1 1 13 7 12 9 – 0 1 1 0 0 0 – – – 1 2 7 0 8 5
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 – – – 0 1 1 0 0 0
Mitochondria 1 8 6 12 29 167 276 250 – 347 270 430 0 0 0 – – – 23 21 96 32 64 56
Nucleus 0 0 3 2 1 0 5 6 – 5 1 3 0 0 1 – – – 0 0 0 0 1 0
Nuclear Periphery 0 0 0 2 0 0 0 2 – 2 1 2 0 0 0 – – – 0 0 2 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 – 2 0 1 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 3 – 0 0 0 0 0 0 – – – 0 0 1 0 0 0
SpindlePole 1 1 1 2 5 3 8 7 – 12 12 14 0 0 0 – – – 1 2 4 0 7 7
Vac/Vac Membrane 2 0 0 1 17 19 33 18 – 17 6 12 0 0 0 – – – 1 3 6 4 20 18
Unique Cell Count 196 274 436 294 506 677 949 904 811 480 780 271 270 382 194 286 442 182 545 505
Labelled Cell Count 201 280 445 306 535 770 1101 1022 998 632 984 277 282 388 194 286 442 182 545 505


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 3.8 3.9 4.3 4.3 3.5 3.3 3.5 – 3.0 3.1 3.0 5.2 5.1 5.3 – 4.1 4.9 5.5 5.5 5.7
Std Deviation (1e-4) 2.1 0.6 1.2 1.9 1.8 1.3 1.3 1.1 – 1.0 1.3 0.9 0.8 0.8 1.0 – 1.2 1.5 1.5 1.7 1.8
Intensity Change (Log2) – – – 0.14 0.14 -0.17 -0.24 -0.13 – -0.36 -0.32 -0.36 0.44 0.4 0.46 – 0.07 0.33 0.5 0.51 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5026 2.0799 2.2047 2.439 1.2167 2.0275 1.6501 1.759 1.3982 1.6667 1.5495 1.2239 0.7869 1.161 1.0763 0.7142 0.1747 0.8851
Actin 0.0093 0.0036 0.0121 0.0125 0.0044 0.0023 0.0969 0.0074 0.0487 0.0035 0.0173 0.0035 0.1002 0.0058 0.0232 0.0495 0.0011 0.0028
Bud 0.0001 0.0003 0.0003 0.0003 0.0002 0.0001 0.0008 0.0002 0.002 0.0137 0.0001 0.0042 0.0007 0.0002 0.0008 0.0012 0 0.0001
Bud Neck 0.0013 0.0008 0.0008 0.0018 0.0015 0.0038 0.0028 0.0005 0.0022 0.0004 0.0004 0.0027 0.0012 0.001 0.0017 0.0012 0.0005 0.001
Bud Periphery 0.0003 0.0006 0.0005 0.0003 0.0003 0.0001 0.001 0.0003 0.0037 0.0356 0.0001 0.0015 0.001 0.0002 0.0007 0.002 0 0.0001
Bud Site 0.0005 0.0117 0.003 0.0008 0.0008 0.0002 0.0025 0.0035 0.0078 0.0017 0.0004 0.0002 0.0048 0.0062 0.0223 0.0037 0 0.0002
Cell Periphery 0.0009 0.003 0.0016 0.0006 0.0011 0.0005 0.0012 0.004 0.0018 0.0012 0.0004 0.0007 0.001 0.0047 0.0029 0.0013 0.0005 0.0002
Cytoplasm 0.3868 0.4234 0.4604 0.4089 0.5247 0.4472 0.2012 0.3638 0.3503 0.1648 0.4757 0.4351 0.229 0.4782 0.3252 0.4591 0.3146 0.3604
Cytoplasmic Foci 0.0235 0.0209 0.0237 0.0069 0.0219 0.016 0.0233 0.0136 0.0381 0.0203 0.0235 0.0098 0.0172 0.0192 0.0381 0.0167 0.0052 0.0195
Eisosomes 0.0007 0.0004 0.0003 0.0002 0.0001 0.0001 0.0006 0.0009 0.0028 0.0004 0.0002 0.0002 0.0007 0.0001 0.0003 0.0002 0.0001 0
Endoplasmic Reticulum 0.1649 0.176 0.1945 0.2754 0.1782 0.2677 0.0941 0.2528 0.2099 0.3232 0.3448 0.238 0.3568 0.1499 0.343 0.2166 0.3388 0.3621
Endosome 0.0236 0.0221 0.0278 0.018 0.0263 0.0146 0.0489 0.0082 0.0214 0.0791 0.0058 0.0129 0.0302 0.0472 0.0182 0.0451 0.0124 0.0234
Golgi 0.0028 0.002 0.0054 0.0012 0.0038 0.0011 0.0181 0.0044 0.0176 0.0318 0.0021 0.002 0.0155 0.0032 0.013 0.0178 0.0013 0.0064
Lipid Particles 0.0107 0.0077 0.0069 0.003 0.0074 0.0033 0.0296 0.014 0.0114 0.008 0.0059 0.0021 0.0195 0.021 0.0074 0.0055 0.0108 0.0141
Mitochondria 0.0016 0.0008 0.001 0.0016 0.0007 0.0008 0.0065 0.0004 0.0075 0.0072 0.0003 0.0162 0.0057 0.0003 0.0324 0.0047 0.0001 0.002
None 0.3489 0.3039 0.2372 0.2354 0.1866 0.2274 0.3777 0.3191 0.2501 0.2912 0.1161 0.26 0.1871 0.2491 0.1501 0.1451 0.3094 0.1913
Nuclear Periphery 0.0103 0.0072 0.0069 0.0156 0.0167 0.0068 0.0596 0.0024 0.0026 0.0055 0.0023 0.0042 0.01 0.0068 0.0049 0.0153 0.0031 0.004
Nucleolus 0.0003 0.0005 0.0005 0.0003 0.0004 0.0001 0.001 0.0002 0.0001 0.0005 0.0001 0.0001 0.0006 0.0001 0.0002 0.0002 0 0.0003
Nucleus 0.0028 0.0043 0.0034 0.0095 0.0113 0.0032 0.0153 0.0008 0.0009 0.0015 0.0007 0.0027 0.0019 0.0014 0.001 0.0029 0.0005 0.0011
Peroxisomes 0.0012 0.0008 0.0015 0.0005 0.0008 0.0003 0.0051 0.0005 0.0171 0.0028 0.0022 0.0002 0.0048 0.0002 0.0022 0.0011 0 0.0016
Punctate Nuclear 0.0037 0.0021 0.0067 0.0022 0.0065 0.0011 0.0054 0.0008 0.0016 0.0014 0.0007 0.001 0.0084 0.0012 0.0099 0.0022 0.0003 0.0074
Vacuole 0.0048 0.0075 0.0049 0.0043 0.0058 0.0031 0.0044 0.0021 0.0022 0.0048 0.0009 0.0024 0.003 0.0036 0.0022 0.0068 0.001 0.0017
Vacuole Periphery 0.0008 0.0006 0.0005 0.0007 0.0005 0.0004 0.004 0.0002 0.0003 0.0014 0.0001 0.0004 0.0009 0.0004 0.0004 0.0019 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.9075 19.9268 20.8596 18.5603 14.1926 18.3003 24.1955 23.9188 21.4851 18.7077
Translational Efficiency 0.481 0.6495 0.3296 0.3165 0.6089 0.478 0.444 0.3607 0.3189 0.4688

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Low-affinity inorganic phosphate (Pi) transporter; acts upstream of Pho81p in regulation of the PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (30%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pho87

Pho87


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pho87-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available