Standard name
Human Ortholog
Description Low-affinity inorganic phosphate (Pi) transporter; acts upstream of Pho81p in regulation of the PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.95 0.97 0.93 0.92 0.83 0.79 0.79 0.72 0.65 0.62 0.97 0.98 0.99 0.66 0.78 0.49 0.62 0.66 0.65
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.05 0.11 0.07 0 0.06
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.06 0.25 0.29 0.28 0.43 0.56 0.55 0 0 0 0.12 0.08 0.22 0.18 0.12 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 3 0 0 0 0 0 0 0 1 0 0 4 4 21 4 12 14
Bud 0 2 3 4 3 7 8 6 18 20 28 0 0 0 2 0 5 2 22 12
Bud Neck 0 1 4 0 1 0 1 2 3 1 1 1 2 2 1 3 5 1 8 8
Bud Site 0 0 0 0 0 0 0 1 1 4 2 0 0 0
Cell Periphery 3 3 1 2 0 4 7 3 4 1 3 4 11 3 1 3 8 4 12 9
Cytoplasm 194 259 423 272 464 560 748 715 587 314 485 262 264 380 127 223 217 112 357 328
Endoplasmic Reticulum 0 6 2 5 1 3 3 0 0 1 2 8 5 2 23 12 48 12 14 28
Endosome 0 0 1 1 13 7 12 9 0 1 1 0 0 0 1 2 7 0 8 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 0 0 0
Mitochondria 1 8 6 12 29 167 276 250 347 270 430 0 0 0 23 21 96 32 64 56
Nucleus 0 0 3 2 1 0 5 6 5 1 3 0 0 1 0 0 0 0 1 0
Nuclear Periphery 0 0 0 2 0 0 0 2 2 1 2 0 0 0 0 0 2 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 3 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 1 1 1 2 5 3 8 7 12 12 14 0 0 0 1 2 4 0 7 7
Vac/Vac Membrane 2 0 0 1 17 19 33 18 17 6 12 0 0 0 1 3 6 4 20 18
Unique Cell Count 196 274 436 294 506 677 949 904 811 480 780 271 270 382 194 286 442 182 545 505
Labelled Cell Count 201 280 445 306 535 770 1101 1022 998 632 984 277 282 388 194 286 442 182 545 505


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 3.8 3.9 4.3 4.3 3.5 3.3 3.5 3.0 3.1 3.0 5.2 5.1 5.3 4.1 4.9 5.5 5.5 5.7
Std Deviation (1e-4) 2.1 0.6 1.2 1.9 1.8 1.3 1.3 1.1 1.0 1.3 0.9 0.8 0.8 1.0 1.2 1.5 1.5 1.7 1.8
Intensity Change (Log2) 0.14 0.14 -0.17 -0.24 -0.13 -0.36 -0.32 -0.36 0.44 0.4 0.46 0.07 0.33 0.5 0.51 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5026 2.0799 2.2047 2.439 1.2167 2.0275 1.6501 1.759 1.3982 1.6667 1.5495 1.2239 0.7869 1.161 1.0763 0.7142 0.1747 0.8851
Actin 0.0093 0.0036 0.0121 0.0125 0.0044 0.0023 0.0969 0.0074 0.0487 0.0035 0.0173 0.0035 0.1002 0.0058 0.0232 0.0495 0.0011 0.0028
Bud 0.0001 0.0003 0.0003 0.0003 0.0002 0.0001 0.0008 0.0002 0.002 0.0137 0.0001 0.0042 0.0007 0.0002 0.0008 0.0012 0 0.0001
Bud Neck 0.0013 0.0008 0.0008 0.0018 0.0015 0.0038 0.0028 0.0005 0.0022 0.0004 0.0004 0.0027 0.0012 0.001 0.0017 0.0012 0.0005 0.001
Bud Periphery 0.0003 0.0006 0.0005 0.0003 0.0003 0.0001 0.001 0.0003 0.0037 0.0356 0.0001 0.0015 0.001 0.0002 0.0007 0.002 0 0.0001
Bud Site 0.0005 0.0117 0.003 0.0008 0.0008 0.0002 0.0025 0.0035 0.0078 0.0017 0.0004 0.0002 0.0048 0.0062 0.0223 0.0037 0 0.0002
Cell Periphery 0.0009 0.003 0.0016 0.0006 0.0011 0.0005 0.0012 0.004 0.0018 0.0012 0.0004 0.0007 0.001 0.0047 0.0029 0.0013 0.0005 0.0002
Cytoplasm 0.3868 0.4234 0.4604 0.4089 0.5247 0.4472 0.2012 0.3638 0.3503 0.1648 0.4757 0.4351 0.229 0.4782 0.3252 0.4591 0.3146 0.3604
Cytoplasmic Foci 0.0235 0.0209 0.0237 0.0069 0.0219 0.016 0.0233 0.0136 0.0381 0.0203 0.0235 0.0098 0.0172 0.0192 0.0381 0.0167 0.0052 0.0195
Eisosomes 0.0007 0.0004 0.0003 0.0002 0.0001 0.0001 0.0006 0.0009 0.0028 0.0004 0.0002 0.0002 0.0007 0.0001 0.0003 0.0002 0.0001 0
Endoplasmic Reticulum 0.1649 0.176 0.1945 0.2754 0.1782 0.2677 0.0941 0.2528 0.2099 0.3232 0.3448 0.238 0.3568 0.1499 0.343 0.2166 0.3388 0.3621
Endosome 0.0236 0.0221 0.0278 0.018 0.0263 0.0146 0.0489 0.0082 0.0214 0.0791 0.0058 0.0129 0.0302 0.0472 0.0182 0.0451 0.0124 0.0234
Golgi 0.0028 0.002 0.0054 0.0012 0.0038 0.0011 0.0181 0.0044 0.0176 0.0318 0.0021 0.002 0.0155 0.0032 0.013 0.0178 0.0013 0.0064
Lipid Particles 0.0107 0.0077 0.0069 0.003 0.0074 0.0033 0.0296 0.014 0.0114 0.008 0.0059 0.0021 0.0195 0.021 0.0074 0.0055 0.0108 0.0141
Mitochondria 0.0016 0.0008 0.001 0.0016 0.0007 0.0008 0.0065 0.0004 0.0075 0.0072 0.0003 0.0162 0.0057 0.0003 0.0324 0.0047 0.0001 0.002
None 0.3489 0.3039 0.2372 0.2354 0.1866 0.2274 0.3777 0.3191 0.2501 0.2912 0.1161 0.26 0.1871 0.2491 0.1501 0.1451 0.3094 0.1913
Nuclear Periphery 0.0103 0.0072 0.0069 0.0156 0.0167 0.0068 0.0596 0.0024 0.0026 0.0055 0.0023 0.0042 0.01 0.0068 0.0049 0.0153 0.0031 0.004
Nucleolus 0.0003 0.0005 0.0005 0.0003 0.0004 0.0001 0.001 0.0002 0.0001 0.0005 0.0001 0.0001 0.0006 0.0001 0.0002 0.0002 0 0.0003
Nucleus 0.0028 0.0043 0.0034 0.0095 0.0113 0.0032 0.0153 0.0008 0.0009 0.0015 0.0007 0.0027 0.0019 0.0014 0.001 0.0029 0.0005 0.0011
Peroxisomes 0.0012 0.0008 0.0015 0.0005 0.0008 0.0003 0.0051 0.0005 0.0171 0.0028 0.0022 0.0002 0.0048 0.0002 0.0022 0.0011 0 0.0016
Punctate Nuclear 0.0037 0.0021 0.0067 0.0022 0.0065 0.0011 0.0054 0.0008 0.0016 0.0014 0.0007 0.001 0.0084 0.0012 0.0099 0.0022 0.0003 0.0074
Vacuole 0.0048 0.0075 0.0049 0.0043 0.0058 0.0031 0.0044 0.0021 0.0022 0.0048 0.0009 0.0024 0.003 0.0036 0.0022 0.0068 0.001 0.0017
Vacuole Periphery 0.0008 0.0006 0.0005 0.0007 0.0005 0.0004 0.004 0.0002 0.0003 0.0014 0.0001 0.0004 0.0009 0.0004 0.0004 0.0019 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.9075 19.9268 20.8596 18.5603 14.1926 18.3003 24.1955 23.9188 21.4851 18.7077
Translational Efficiency 0.481 0.6495 0.3296 0.3165 0.6089 0.478 0.444 0.3607 0.3189 0.4688

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
202 1279 176 128 1901 2362 1329 3201 2103 3641 1505 3329

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 833.35 704.37 907.54 1068.34 608.68 695.37 886.50 995.20 630.26 698.53 888.96 998.01
Standard Deviation 127.51 97.39 103.68 141.88 68.24 98.04 104.20 509.27 100.77 97.91 104.36 500.36
Intensity Change Log 2 -0.242589 0.123039 0.358376 0.192097 0.542437 0.709303 -0.042942 0.315111 0.517020

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.005559 0.000189 0.000819 0.003182 0.000167 0.000392 0.002668 0.002341 0.000685 0.000321 0.002452 0.002373
Bud Neck 0.008713 0.013016 0.002127 0.007703 0.025147 0.032683 0.005069 0.015018 0.023569 0.025774 0.004725 0.014736
Bud Site 0.037387 0.005049 0.004869 0.036335 0.004916 0.011755 0.018062 0.023488 0.008035 0.009399 0.016519 0.023982
Cell Periphery 0.000734 0.000121 0.000402 0.001638 0.000222 0.000221 0.001086 0.001126 0.000272 0.000186 0.001006 0.001146
Cytoplasm 0.294550* 0.516945* 0.441108* 0.111141* 0.424796* 0.367949* 0.231216* 0.301799* 0.412285* 0.420288* 0.255762* 0.294469*
Cytoplasmic Foci 0.113081 0.209306* 0.076046 0.058536 0.293280* 0.247229* 0.041837 0.029659 0.275971* 0.233908* 0.045837 0.030770
Eisosomes 0.000228 0.000104 0.000114 0.000453 0.000403 0.000329 0.000340 0.000740 0.000386 0.000250 0.000313 0.000729
Endoplasmic Reticulum 0.012349 0.004655 0.016770 0.222250* 0.003149 0.020385 0.425506* 0.336564* 0.004032 0.014859 0.377707* 0.332168*
Endosome 0.030638 0.011964 0.015323 0.027014 0.009488 0.026429 0.015749 0.011807 0.011520 0.021348 0.015700 0.012392
Golgi 0.011265 0.002245 0.000268 0.010686 0.006441 0.007557 0.003864 0.005502 0.006904 0.005691 0.003444 0.005701
Lipid Particles 0.009529 0.010775 0.011035 0.054105 0.021330 0.037044 0.039225 0.050385 0.020197 0.027816 0.035928 0.050528
Mitochondria 0.012168 0.003156 0.000173 0.008653 0.009905 0.015097 0.000898 0.006444 0.010123 0.010903 0.000813 0.006529
Mitotic Spindle 0.006686 0.004271 0.000218 0.018573 0.000099 0.003402 0.002643 0.002437 0.000732 0.003707 0.002360 0.003058
None 0.013775 0.005035 0.001375 0.000458 0.003431 0.001809 0.000327 0.009218 0.004425 0.002942 0.000449 0.008881
Nuclear Periphery 0.000964 0.001342 0.002810 0.002340 0.000483 0.001945 0.006395 0.005722 0.000529 0.001733 0.005976 0.005592
Nuclear Periphery Foci 0.004911 0.002024 0.046010 0.088355 0.000306 0.001516 0.047397 0.016691 0.000748 0.001695 0.047235 0.019447
Nucleolus 0.000846 0.002172 0.000601 0.000163 0.000915 0.001765 0.000203 0.000891 0.000909 0.001908 0.000250 0.000863
Nucleus 0.122551 0.147315* 0.177856* 0.021814 0.154785* 0.115937 0.059615 0.114048 0.151689* 0.126959 0.073443 0.110501
Peroxisomes 0.007155 0.005483 0.000746 0.001336 0.006389 0.010103 0.001282 0.002506 0.006462 0.008480 0.001220 0.002461
Vacuole 0.298769* 0.053733 0.201032* 0.323622* 0.032973 0.093601 0.095886 0.062296 0.058503 0.079596 0.108182 0.072344
Vacuole Periphery 0.008141 0.001101 0.000296 0.001643 0.001375 0.002854 0.000732 0.001318 0.002025 0.002238 0.000681 0.001330

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.36 1.13 0.25 -3.29 -2.57 -5.50 -5.27 -8.17 -7.27 0.66 0.82 -3.77 -4.75 -7.95 0.21
Bud Neck -2.05 2.15 0.10 3.76 -4.76 -3.43 12.26 5.62 9.43 -8.79 -1.28 12.48 5.19 7.52 -9.36
Bud Site 3.26 3.20 -0.50 -3.31 -3.26 -5.91 -5.62 -11.19 -6.22 -1.65 -1.70 -4.23 -9.19 -8.52 -2.58
Cell Periphery 2.02 0.87 -2.36 -5.20 -4.06 0.17 -8.90 -17.00 -17.14 -0.24 2.77 -8.37 -15.45 -18.60 -1.20
Cytoplasm -10.94 -4.71 6.59 22.04 11.89 7.66 23.08 17.82 9.52 -9.29 -0.96 19.36 17.18 20.15 -5.71
Cytoplasmic Foci -10.12 2.87 4.30 17.34 2.00 7.17 47.92 52.61 50.37 5.07 7.10 45.81 50.99 57.52 6.48
Eisosomes 4.00 3.12 -2.54 -4.17 -3.89 2.69 1.87 -6.98 -8.19 -7.46 6.33 2.34 -7.43 -10.52 -8.19
Endoplasmic Reticulum 1.25 -0.99 -6.24 -6.59 -6.13 -8.74 -42.42* -53.74* -48.80* 7.73 -7.55 -39.60 -53.64 -50.98* 4.36
Endosome 2.20 1.74 0.29 -3.13 -2.28 -14.25 -6.25 -3.19 12.31 3.95 -9.90 -4.35 -1.40 10.01 3.65
Golgi 2.07 2.77 0.11 -0.78 -1.07 -1.57 3.82 1.99 3.52 -2.43 1.60 4.78 1.89 0.46 -3.22
Lipid Particles -1.28 -0.83 -4.58 -4.35 -4.40 -8.06 -8.05 -14.01 -5.77 -4.46 -5.11 -7.85 -15.12 -11.18 -6.10
Mitochondria 1.72 2.54 0.44 -0.51 -1.00 -4.14 11.58 4.30 8.53 -15.15 -0.98 11.53 4.08 5.99 -13.29
Mitotic Spindle 0.35 1.89 -1.18 -1.35 -1.87 -4.53 -3.60 -5.37 0.65 0.03 -4.59 -2.72 -4.30 0.48 -0.83
None 0.79 1.32 1.45 5.14 2.29 3.95 9.37 -5.28 -7.00 -8.71 1.47 5.01 -3.74 -5.64 -8.58
Nuclear Periphery -0.70 -4.13 -2.25 -1.58 0.94 -5.88 -14.15 -12.53 -7.91 0.91 -6.19 -14.53 -12.53 -8.80 0.46
Nuclear Periphery Foci 2.40 -8.15 -6.94 -7.24 -2.77 -3.74 -21.75 -18.80 -16.38 12.90 -3.76 -23.23 -19.23 -17.78 12.50
Nucleolus -3.18 0.62 2.65 4.73 4.33 -3.26 13.70 -0.04 3.24 -12.81 -4.51 12.41 0.12 4.53 -11.78
Nucleus -1.16 -2.11 7.37 21.28 10.68 8.77 20.67 9.08 0.14 -13.41 6.13 16.85 9.54 4.28 -9.04
Peroxisomes 0.02 2.08 1.86 7.38 -1.47 -5.16 11.11 8.12 12.75 -6.16 -3.60 10.80 7.87 13.69 -6.60
Vacuole 12.72 3.51 -1.90 -12.71 -5.11 -21.67 -16.06 -14.78 7.13 7.01 -5.11 -10.71 -5.00 -0.54 7.26
Vacuole Periphery 2.38 2.76 2.23 -0.23 -1.52 -6.20 3.07 0.42 6.28 -2.50 -1.32 4.15 1.92 4.37 -2.93