ORF
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.68 0.84 0.90 0.89 0.85 0.80 0.84 0.80 0.78 0.67 0.78 0.96 0.86 0.81 0.11 0.15 0.12 0.19 0.27 0.24 0.42 0.50 0.43
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0.07 0 0 0.05 0.08 0.06 0 0 0 0 0 0 0.11 0.12 0.14 0.17 0.24 0.21 0.10 0.06 0 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.40 0.36 0.37 0 0 0 0 0 0
Mitochondria 0.22 0.05 0 0 0 0.05 0.07 0.18 0.09 0.40 0.15 0 0 0 0.10 0.09 0.13 0.20 0.24 0.30 0.21 0.18 0.17
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.06 0.05 0 0 0.06 0 0 0 0.08 0.06 0.06 0.07 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0.07 0 0 0 0 0 0.06 0 0.05 0 0 0 0.53 0.46 0.45 0.26 0.16 0.24 0.13 0.15 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 2 2 5 4 3 5 0 2 0 0 0 6 1 15 7 2 3 4 4 1 3 9
Bud 3 2 1 3 1 4 7 6 6 7 2 0 1 1 2 0 3 0 0 1 0 2 6
Bud Neck 1 0 4 2 3 5 11 6 13 4 6 0 2 2 0 0 0 0 2 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0
Cell Periphery 0 1 0 1 1 0 1 2 1 0 1 1 1 0 1 2 0 0 0 0 0 0 0
Cytoplasm 117 112 78 87 183 180 296 177 221 131 161 23 205 209 39 43 26 41 43 29 22 121 139
Endoplasmic Reticulum 3 3 0 0 1 3 0 0 1 3 2 1 4 4 17 8 12 1 0 1 0 2 3
Endosome 6 9 1 3 10 19 20 5 11 0 3 0 4 28 44 40 37 53 33 12 3 10 25
Golgi 0 0 0 0 0 1 0 0 2 0 0 0 2 3 141 102 81 5 3 1 0 0 2
Mitochondria 38 7 1 0 1 11 24 40 26 78 32 0 0 3 37 25 28 44 39 36 11 44 55
Nucleus 0 1 0 0 0 0 0 2 2 2 2 0 0 2 1 3 1 0 0 0 0 1 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 2 0 0 0 1 5 8 1 0 0 0 0 0 0
Peroxisomes 0 1 0 1 0 1 5 0 1 0 0 0 1 8 0 1 3 5 2 1 0 0 1
SpindlePole 3 2 0 4 13 11 15 8 18 8 9 0 18 15 22 20 9 4 2 0 1 9 9
Vac/Vac Membrane 9 4 6 1 7 6 11 9 17 4 10 0 2 5 187 128 99 57 26 30 7 35 61
Unique Cell Count 173 134 87 98 216 224 353 222 283 196 207 24 239 259 356 280 219 222 161 123 55 241 327
Labelled Cell Count 182 144 93 107 224 244 395 255 321 241 229 25 246 282 512 388 302 222 161 123 55 241 327

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Punctate Nuclear

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.9 5.4 5.2 5.8 4.6 4.7 3.9 4.3 3.4 3.8 5.1 5.5 5.6 12.2 12.5 11.3 4.8 5.2 5.5
Std Deviation (1e-4) 0.5 1.0 1.6 1.8 1.7 1.2 1.5 0.8 1.5 0.8 0.8 0.7 1.1 1.6 2.5 2.5 2.5 1.0 1.4 1.3
Intensity Change (Log2) -0.05 0.1 -0.25 -0.22 -0.49 -0.33 -0.68 -0.5 -0.09 0.03 0.04 1.17 1.21 1.06 -0.18 -0.05 0.02

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.7 1.5 0 0 0 0 0 0 0 1.3 1.9 0.8 1.1 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 2.8 3.1 3.4 3.8
Golgi 0 0 0 0 0 0 0 0 0 0 0 7.1 6.6 6.6
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 2.8 2.5 3.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 2.1 0 2.3