Standard name | |
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Human Ortholog | |
Description | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 2 | 2 | 5 | 4 | 3 | 5 | – | 0 | 2 | 0 | 0 | 0 | 6 | 1 | 15 | 7 | 2 | 3 | 4 | 4 | 1 | 3 | 9 |
Bud | 3 | 2 | 1 | 3 | 1 | 4 | 7 | – | 6 | 6 | 7 | 2 | 0 | 1 | 1 | 2 | 0 | 3 | 0 | 0 | 1 | 0 | 2 | 6 |
Bud Neck | 1 | 0 | 4 | 2 | 3 | 5 | 11 | – | 6 | 13 | 4 | 6 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 1 | 1 | 0 | 1 | – | 2 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 117 | 112 | 78 | 87 | 183 | 180 | 296 | – | 177 | 221 | 131 | 161 | 23 | 205 | 209 | 39 | 43 | 26 | 41 | 43 | 29 | 22 | 121 | 139 |
Endoplasmic Reticulum | 3 | 3 | 0 | 0 | 1 | 3 | 0 | – | 0 | 1 | 3 | 2 | 1 | 4 | 4 | 17 | 8 | 12 | 1 | 0 | 1 | 0 | 2 | 3 |
Endosome | 6 | 9 | 1 | 3 | 10 | 19 | 20 | – | 5 | 11 | 0 | 3 | 0 | 4 | 28 | 44 | 40 | 37 | 53 | 33 | 12 | 3 | 10 | 25 |
Golgi | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 2 | 0 | 0 | 0 | 2 | 3 | 141 | 102 | 81 | 5 | 3 | 1 | 0 | 0 | 2 |
Mitochondria | 38 | 7 | 1 | 0 | 1 | 11 | 24 | – | 40 | 26 | 78 | 32 | 0 | 0 | 3 | 37 | 25 | 28 | 44 | 39 | 36 | 11 | 44 | 55 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 2 | 2 | 2 | 2 | 0 | 0 | 2 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 5 | 8 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1 | 0 | 1 | 0 | 1 | 5 | – | 0 | 1 | 0 | 0 | 0 | 1 | 8 | 0 | 1 | 3 | 5 | 2 | 1 | 0 | 0 | 1 |
SpindlePole | 3 | 2 | 0 | 4 | 13 | 11 | 15 | – | 8 | 18 | 8 | 9 | 0 | 18 | 15 | 22 | 20 | 9 | 4 | 2 | 0 | 1 | 9 | 9 |
Vac/Vac Membrane | 9 | 4 | 6 | 1 | 7 | 6 | 11 | – | 9 | 17 | 4 | 10 | 0 | 2 | 5 | 187 | 128 | 99 | 57 | 26 | 30 | 7 | 35 | 61 |
Unique Cell Count | 173 | 134 | 87 | 98 | 216 | 224 | 353 | 222 | 283 | 196 | 207 | 24 | 239 | 259 | 356 | 280 | 219 | 222 | 161 | 123 | 55 | 241 | 327 | |
Labelled Cell Count | 182 | 144 | 93 | 107 | 224 | 244 | 395 | 255 | 321 | 241 | 229 | 25 | 246 | 282 | 512 | 388 | 302 | 222 | 161 | 123 | 55 | 241 | 327 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 4.9 | 5.4 | 5.2 | 5.8 | 4.6 | 4.7 | – | 3.9 | 4.3 | 3.4 | 3.8 | 5.1 | 5.5 | 5.6 | 12.2 | 12.5 | 11.3 | 4.8 | 5.2 | 5.5 |
Std Deviation (1e-4) | 0.5 | 1.0 | 1.6 | 1.8 | 1.7 | 1.2 | 1.5 | – | 0.8 | 1.5 | 0.8 | 0.8 | 0.7 | 1.1 | 1.6 | 2.5 | 2.5 | 2.5 | 1.0 | 1.4 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.1 | -0.25 | -0.22 | – | -0.49 | -0.33 | -0.68 | -0.5 | -0.09 | 0.03 | 0.04 | 1.17 | 1.21 | 1.06 | -0.18 | -0.05 | 0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.2 | -1.1 | -2.0 | -1.4 | – | -2.1 | -2.4 | -4.0 | -2.4 | 0.9 | -0.9 | -1.9 | -14.9 | -12.9 | -13.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 3.1 | 3.4 | 3.8 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7.1 | 6.6 | 6.6 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 2.5 | 3.1 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | -0.3 | 0 | 0 | 0 | 0 | 0 | 7.7 | 6.6 | 6.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.8894 | -0.6689 | -0.7384 | -1.002 | -0.8039 | -0.7442 | 0.3934 | 0.9824 | 0.5965 | 0.464 | 0.4567 | 0.6265 | -0.4398 | -0.6105 | -0.571 | 0.4214 | -0.0645 | -0.585 |
Actin | 0.0193 | 0.0044 | 0.0041 | 0.0355 | 0.0006 | 0.0273 | 0.0061 | 0.0125 | 0.0095 | 0.0011 | 0.0105 | 0.0063 | 0.0227 | 0.0096 | 0.0128 | 0.011 | 0.0604 | 0.007 |
Bud | 0.0007 | 0.0014 | 0.0113 | 0.0022 | 0.0004 | 0.0041 | 0.0004 | 0.0022 | 0.0009 | 0.0017 | 0.0005 | 0.0005 | 0.0007 | 0.0004 | 0.0007 | 0.0018 | 0.0004 | 0.0001 |
Bud Neck | 0.001 | 0.0006 | 0.005 | 0.0007 | 0.0266 | 0.0091 | 0.0007 | 0.0008 | 0.0006 | 0.0008 | 0.0005 | 0.0042 | 0.0013 | 0.0003 | 0.0075 | 0.0008 | 0.0008 | 0.001 |
Bud Periphery | 0.002 | 0.0045 | 0.0027 | 0.0031 | 0.0009 | 0.0037 | 0.0007 | 0.0037 | 0.0024 | 0.0024 | 0.0007 | 0.001 | 0.0008 | 0.0003 | 0.0005 | 0.0023 | 0.0004 | 0.0002 |
Bud Site | 0.004 | 0.0054 | 0.0071 | 0.0035 | 0.0819 | 0.0021 | 0.0018 | 0.009 | 0.0016 | 0.0028 | 0.0016 | 0.0004 | 0.0022 | 0.0025 | 0.0032 | 0.009 | 0.0011 | 0.0002 |
Cell Periphery | 0.0006 | 0.0006 | 0.0005 | 0.0005 | 0.0021 | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0001 |
Cytoplasm | 0.0441 | 0.0518 | 0.0534 | 0.1131 | 0.0549 | 0.056 | 0.0609 | 0.0805 | 0.0578 | 0.0355 | 0.0276 | 0.0705 | 0.0605 | 0.1358 | 0.0696 | 0.0348 | 0.0765 | 0.0968 |
Cytoplasmic Foci | 0.1616 | 0.177 | 0.171 | 0.246 | 0.0552 | 0.1621 | 0.2339 | 0.2204 | 0.2434 | 0.2638 | 0.2365 | 0.2132 | 0.2063 | 0.2165 | 0.2147 | 0.141 | 0.2321 | 0.2168 |
Eisosomes | 0.0008 | 0.0022 | 0.0004 | 0.0004 | 0.0005 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0011 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0036 | 0.003 | 0.007 | 0.0067 | 0.0078 | 0.0027 | 0.002 | 0.0021 | 0.0022 | 0.0009 | 0.0018 | 0.0014 | 0.0058 | 0.0031 | 0.0028 | 0.0012 | 0.0028 | 0.0031 |
Endosome | 0.1247 | 0.1648 | 0.197 | 0.2517 | 0.2562 | 0.093 | 0.2018 | 0.23 | 0.2158 | 0.2831 | 0.2064 | 0.1729 | 0.2248 | 0.2406 | 0.2124 | 0.3375 | 0.2528 | 0.2259 |
Golgi | 0.0436 | 0.0209 | 0.0444 | 0.0452 | 0.0051 | 0.0315 | 0.0545 | 0.0513 | 0.0505 | 0.0472 | 0.063 | 0.0583 | 0.0359 | 0.0353 | 0.0346 | 0.0732 | 0.0562 | 0.0481 |
Lipid Particles | 0.0935 | 0.0481 | 0.0564 | 0.0634 | 0.09 | 0.0439 | 0.0534 | 0.0347 | 0.0262 | 0.0363 | 0.0669 | 0.0285 | 0.0771 | 0.0403 | 0.0282 | 0.0729 | 0.081 | 0.0406 |
Mitochondria | 0.0515 | 0.0445 | 0.0249 | 0.0227 | 0.0189 | 0.0351 | 0.021 | 0.0391 | 0.0319 | 0.0331 | 0.0292 | 0.0278 | 0.0164 | 0.0119 | 0.0102 | 0.0121 | 0.0042 | 0.007 |
None | 0.3659 | 0.4211 | 0.316 | 0.1707 | 0.3428 | 0.444 | 0.2322 | 0.2068 | 0.2182 | 0.1601 | 0.1913 | 0.2493 | 0.23 | 0.2537 | 0.3389 | 0.2506 | 0.1091 | 0.2743 |
Nuclear Periphery | 0.0062 | 0.0014 | 0.0023 | 0.0019 | 0.0075 | 0.0006 | 0.0011 | 0.0019 | 0.0013 | 0.0006 | 0.0007 | 0.0026 | 0.0142 | 0.0015 | 0.0026 | 0.0018 | 0.003 | 0.0038 |
Nucleolus | 0.0022 | 0.0008 | 0.0014 | 0.0004 | 0.0066 | 0.0006 | 0.0018 | 0.0018 | 0.0008 | 0.0004 | 0.0003 | 0.0005 | 0.0017 | 0.0004 | 0.0015 | 0.0101 | 0.0009 | 0.0003 |
Nucleus | 0.0047 | 0.0009 | 0.0014 | 0.0011 | 0.0034 | 0.0006 | 0.0028 | 0.0035 | 0.0014 | 0.0008 | 0.0004 | 0.0014 | 0.0039 | 0.0012 | 0.0015 | 0.0027 | 0.0014 | 0.0009 |
Peroxisomes | 0.0458 | 0.035 | 0.0589 | 0.0144 | 0.01 | 0.071 | 0.1126 | 0.0802 | 0.122 | 0.1154 | 0.1545 | 0.1498 | 0.0671 | 0.0318 | 0.041 | 0.0241 | 0.062 | 0.0637 |
Punctate Nuclear | 0.0071 | 0.0017 | 0.0159 | 0.0039 | 0.0025 | 0.0033 | 0.004 | 0.0058 | 0.0048 | 0.0017 | 0.0022 | 0.006 | 0.0175 | 0.0017 | 0.008 | 0.0049 | 0.0496 | 0.0031 |
Vacuole | 0.0127 | 0.0083 | 0.0157 | 0.0096 | 0.02 | 0.0057 | 0.0063 | 0.0104 | 0.0061 | 0.0099 | 0.0035 | 0.0037 | 0.0068 | 0.01 | 0.0064 | 0.0055 | 0.0035 | 0.0049 |
Vacuole Periphery | 0.0045 | 0.0017 | 0.0031 | 0.0032 | 0.0059 | 0.0025 | 0.0016 | 0.0027 | 0.0021 | 0.0022 | 0.0014 | 0.0016 | 0.0031 | 0.0026 | 0.0021 | 0.0021 | 0.0016 | 0.0019 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 36.6638 | 33.8156 | 29.7252 | 29.8544 | 26.0317 | 57.0009 | 55.4946 | 56.8039 | 49.9437 | 39.8509 |
Translational Efficiency | 1.1281 | 1.0839 | 1.215 | 1.2816 | 1.0122 | 1.0381 | 1.0826 | 1.0897 | 1.4139 | 0.9949 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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