Standard name
Description Component of the SF3b subcomplex of the U2 snRNP; increases efficiency of first and second step pre-mRNA splicing; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.06 0.1 0.05 0.08 0 0.06 0 0.1 0.14 0.13 0.11 0.11 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.14 0.16 0.13 0.13 0.19 0 0 0 0 0.07 0 0 0 0 0 0 0
Nucleus 0.97 0.96 0.91 0.85 0.88 0.77 0.64 0.73 0.77 0.72 0.74 0.87 0.77 0.67 0.72 0.71 0.75 0.96 0.95 0.87 0.82 0.64 0.48
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.19 0.09 0.15 0.26 0.26 0.25 0.26 0.26 0.07 0.16 0.2 0.08 0.07 0.1 0 0 0 0.06 0.11 0.12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.09 0.06 0 0 0.06 0.07 0.18 0.29
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Bud 0 0 0 0 1 0 1 0 1 0 0 0 0 0 1 1 1 1 0 0 0 2 5
Bud Neck 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 3 1 6 5 3 1 1 3 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 9 2 3 3 6 13 10 10 8 6 3 24 28 34 30 22 20 0 1 0 3 1 3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 2 6 0 0 0 0 1 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 1 0 5 3 2 3 0 1 0 1 1 3
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 0 3 2
Mitochondria 1 2 2 1 0 10 29 21 24 13 26 2 0 2 6 15 4 0 0 1 0 1 4
Nucleus 362 207 77 50 90 99 129 94 138 71 103 217 158 171 201 144 177 363 213 86 139 164 136
Nuclear Periphery 0 0 0 0 0 4 6 1 1 0 4 2 0 4 5 3 2 0 0 0 0 0 2
Nucleolus 2 4 2 11 9 19 52 34 44 26 36 17 33 51 22 14 24 2 0 1 10 28 33
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 7 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 1 2 8 1 2 1 0 0 0 0 0 2
Vac/Vac Membrane 4 3 1 0 1 2 4 1 1 0 1 2 6 7 31 18 15 8 5 5 12 44 81
Unique Cell Count 375 216 85 59 102 129 201 129 179 99 140 249 205 256 279 202 237 378 225 100 170 255 282
Labelled Cell Count 378 219 85 65 107 147 236 163 223 121 177 267 230 292 303 224 255 378 225 100 170 255 282


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.0 15.2 8.9 11.4 10.2 7.9 7.4 6.5 6.9 7.0 6.6 14.0 13.0 12.5 14.8 12.9 13.1 12.2 13.2 12.9
Std Deviation (1e-4) 3.8 3.8 3.2 3.2 3.3 2.5 2.9 2.3 2.4 3.5 2.2 4.6 4.5 5.0 4.7 4.5 4.1 3.2 3.6 4.4
Intensity Change (Log2) 0.36 0.2 -0.17 -0.26 -0.44 -0.36 -0.33 -0.43 0.66 0.55 0.49 0.73 0.54 0.57 0.46 0.58 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 1.8 0 1.3 0 0 0 1.8 2.5 2.5 2.0 2.0 1.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.0 3.2 2.8 2.7 3.6 0 0 0 0 1.7 0
Nucleus -1.1 -0.5 -2.6 -4.5 -3.2 -2.6 -3.2 -3.1 -0.8 -2.7 -4.3 -3.5 -3.5 -3.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 3.4 1.9 3.0 4.6 4.6 4.4 4.5 4.5 0 3.3 3.9 1.8 0 2.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 2.8 2.4 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bud31-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available