Standard name | |
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Description | Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; binds to mRNAs under glucose starvation, most often in the 3' UTR; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress; phosphorylation by PKA inhibits P body foci formation |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 2 | 2 | 3 | 5 | 9 | 15 | 13 | – | 8 | 15 | 6 | 24 | 19 | 0 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 2 | 4 | 3 | 6 | 11 | 9 | 10 | 6 | – | 6 | 3 | 2 | 12 | 21 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 3 | 10 | 10 | 11 | 11 | 8 | 6 | 5 | – | 9 | 10 | 2 | 4 | 4 | 24 | 21 | 42 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 143 | 198 | 209 | 196 | 233 | 165 | 230 | 203 | 112 | – | 105 | 107 | 82 | 120 | 114 | 299 | 301 | 259 | 143 | 218 | 213 | 112 | 146 | 167 |
Endoplasmic Reticulum | 4 | 15 | 2 | 0 | 10 | 7 | 14 | 4 | 11 | – | 31 | 19 | 19 | 14 | 19 | 15 | 5 | 4 | 10 | 7 | 8 | 33 | 40 | 42 |
Endosome | 0 | 0 | 2 | 2 | 2 | 6 | 8 | 16 | 2 | – | 1 | 2 | 1 | 24 | 50 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 0 | – | 0 | 2 | 6 | 31 | 38 | 4 | 2 | 4 | 0 | 1 | 0 | 0 | 1 | 1 |
Mitochondria | 1 | 7 | 6 | 26 | 24 | 62 | 121 | 91 | 111 | – | 130 | 92 | 8 | 9 | 17 | 4 | 3 | 6 | 2 | 0 | 6 | 2 | 5 | 3 |
Nucleus | 1 | 0 | 0 | 2 | 1 | 0 | 3 | 3 | 1 | – | 0 | 1 | 0 | 0 | 0 | 2 | 3 | 2 | 0 | 1 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 3 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 2 | 1 | 18 | 8 | 20 | 27 | 47 | 102 | 32 | – | 14 | 36 | 0 | 2 | 3 | 8 | 17 | 5 | 3 | 7 | 9 | 4 | 7 | 9 |
Unique Cell Count | 151 | 215 | 228 | 219 | 282 | 250 | 376 | 385 | 231 | 244 | 239 | 114 | 205 | 248 | 329 | 332 | 300 | 163 | 244 | 247 | 158 | 207 | 228 | |
Labelled Cell Count | 154 | 226 | 253 | 250 | 312 | 294 | 450 | 453 | 295 | 304 | 287 | 127 | 246 | 307 | 357 | 357 | 326 | 163 | 244 | 247 | 158 | 207 | 228 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.0 | 11.7 | 9.5 | 8.8 | 9.5 | 7.8 | 7.8 | 8.3 | 7.3 | – | 7.0 | 7.5 | 10.8 | 11.4 | 11.7 | 16.4 | 15.9 | 16.2 | 10.0 | 10.1 | 10.7 |
Std Deviation (1e-4) | 1.5 | 2.4 | 1.3 | 1.0 | 1.2 | 1.2 | 1.3 | 1.3 | 1.3 | – | 1.4 | 1.4 | 1.8 | 1.4 | 1.6 | 2.9 | 2.9 | 2.8 | 1.4 | 1.5 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.01 | -0.3 | -0.28 | -0.2 | -0.38 | – | -0.45 | -0.35 | 0.18 | 0.26 | 0.29 | 0.78 | 0.74 | 0.77 | 0.07 | 0.08 | 0.16 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 2.2 | 2.9 | – | 0 | 3.1 | 0 | 4.7 | 3.6 | 0 | 0 | 0 |
Bud | 0 | 0 | 1.7 | 0 | 0.7 | 0 | – | 0 | 0 | 0 | 2.3 | 3.3 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.1 | -0.3 | 0 | -1.6 | -2.1 | -1.3 | – | -0.4 | -0.1 | 0 | -1.4 | -1.8 | 1.4 | 1.0 | 3.7 |
Cytoplasm | -0.8 | -3.0 | -6.8 | -8.2 | -9.9 | -10.1 | – | -11.2 | -10.8 | -4.8 | -8.1 | -10.7 | -0.3 | -0.4 | -1.9 |
Endoplasmic Reticulum | 0 | 2.0 | 0 | 2.1 | 0 | 2.5 | – | 5.0 | 3.7 | 5.7 | 3.3 | 3.6 | 2.5 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 2.3 | 0 | – | 0 | 0 | 0 | 4.7 | 6.7 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 6.1 | 6.2 | 0 | 0 | 0 |
Mitochondria | 3.8 | 2.8 | 6.9 | 8.6 | 6.9 | 11.2 | – | 12.1 | 9.5 | 0 | 1.0 | 2.1 | 0 | 0 | -0.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3.4 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.9 | -0.3 | 1.1 | 1.8 | 5.6 | 2.0 | – | -0.9 | 2.4 | -3.1 | -3.4 | -3.5 | -3.0 | -1.3 | -3.5 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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