Standard name
Human Ortholog
Description Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; binds to mRNAs under glucose starvation, most often in the 3' UTR; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress; phosphorylation by PKA inhibits P body foci formation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.06 – 0 0.06 0.05 0.12 0.08 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0.08 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0.07 0.06 0.14 0 0 0 0 0 0
Cytoplasm 0.95 0.92 0.92 0.89 0.83 0.66 0.61 0.53 0.48 – 0.43 0.45 0.72 0.59 0.46 0.91 0.91 0.86 0.88 0.9 0.87 0.71 0.71 0.73
Endoplasmic Reticulum 0 0.07 0 0 0 0 0 0 0.05 – 0.13 0.08 0.17 0.07 0.08 0.05 0 0 0.06 0 0 0.21 0.19 0.19
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0.12 0.2 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0.15 0.15 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.12 0.09 0.25 0.32 0.24 0.48 – 0.53 0.38 0.07 0 0.07 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.08 0 0.07 0.11 0.12 0.26 0.14 – 0.06 0.15 0 0 0 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 2 3 5 9 15 13 – 8 15 6 24 19 0 4 3 0 0 0 0 0 0
Bud 0 2 4 3 6 11 9 10 6 – 6 3 2 12 21 0 0 1 0 2 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 2 0 0 0
Bud Site 0 0 0 0 0 0 1 0 0 – 0 0 0 1 0 0 0 0 – – – – – –
Cell Periphery 2 3 10 10 11 11 8 6 5 – 9 10 2 4 4 24 21 42 0 0 0 0 0 0
Cytoplasm 143 198 209 196 233 165 230 203 112 – 105 107 82 120 114 299 301 259 143 218 213 112 146 167
Endoplasmic Reticulum 4 15 2 0 10 7 14 4 11 – 31 19 19 14 19 15 5 4 10 7 8 33 40 42
Endosome 0 0 2 2 2 6 8 16 2 – 1 2 1 24 50 0 0 0 0 0 1 1 1 0
Golgi 0 0 0 0 2 0 0 2 0 – 0 2 6 31 38 4 2 4 0 1 0 0 1 1
Mitochondria 1 7 6 26 24 62 121 91 111 – 130 92 8 9 17 4 3 6 2 0 6 2 5 3
Nucleus 1 0 0 2 1 0 3 3 1 – 0 1 0 0 0 2 3 2 0 1 1 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 1 1 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 – 0 0 0 0 1 1 1 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 2 12 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 – 0 0 0 3 9 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 1 18 8 20 27 47 102 32 – 14 36 0 2 3 8 17 5 3 7 9 4 7 9
Unique Cell Count 151 215 228 219 282 250 376 385 231 244 239 114 205 248 329 332 300 163 244 247 158 207 228
Labelled Cell Count 154 226 253 250 312 294 450 453 295 304 287 127 246 307 357 357 326 163 244 247 158 207 228


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.0 11.7 9.5 8.8 9.5 7.8 7.8 8.3 7.3 – 7.0 7.5 10.8 11.4 11.7 16.4 15.9 16.2 10.0 10.1 10.7
Std Deviation (1e-4) 1.5 2.4 1.3 1.0 1.2 1.2 1.3 1.3 1.3 – 1.4 1.4 1.8 1.4 1.6 2.9 2.9 2.8 1.4 1.5 1.4
Intensity Change (Log2) – – – -0.11 -0.01 -0.3 -0.28 -0.2 -0.38 – -0.45 -0.35 0.18 0.26 0.29 0.78 0.74 0.77 0.07 0.08 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 2.2 2.9 – 0 3.1 0 4.7 3.6 0 0 0
Bud 0 0 1.7 0 0.7 0 – 0 0 0 2.3 3.3 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0.1 -0.3 0 -1.6 -2.1 -1.3 – -0.4 -0.1 0 -1.4 -1.8 1.4 1.0 3.7
Cytoplasm -0.8 -3.0 -6.8 -8.2 -9.9 -10.1 – -11.2 -10.8 -4.8 -8.1 -10.7 -0.3 -0.4 -1.9
Endoplasmic Reticulum 0 2.0 0 2.1 0 2.5 – 5.0 3.7 5.7 3.3 3.6 2.5 0 0
Endosome 0 0 0 0 2.3 0 – 0 0 0 4.7 6.7 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 6.1 6.2 0 0 0
Mitochondria 3.8 2.8 6.9 8.6 6.9 11.2 – 12.1 9.5 0 1.0 2.1 0 0 -0.5
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 3.4 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole -1.9 -0.3 1.1 1.8 5.6 2.0 – -0.9 2.4 -3.1 -3.4 -3.5 -3.0 -1.3 -3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.8289 11.4378 10.1369 8.5425 8.1503 10.0127 7.4177 10.5373 9.4806 8.946 8.2846 9.1946 6.3507 9.3889 8.5362 7.371 7.1834 8.2778
Actin 0.0153 0.002 0.0022 0.0017 0.0106 0.0013 0.0185 0.0011 0.0038 0.0046 0.0063 0.006 0.0281 0.0017 0.0062 0.0386 0.0307 0.0016
Bud 0.0008 0.001 0.0011 0.0015 0.001 0.0007 0.0011 0.0008 0.0012 0.001 0.0019 0.0005 0.0009 0.0006 0.0009 0.0004 0.0049 0.0004
Bud Neck 0.0008 0.0004 0.0008 0.001 0.0029 0.0015 0.001 0.0003 0.0008 0.0008 0.0013 0.0012 0.0043 0.0002 0.0005 0.0026 0.0011 0.001
Bud Periphery 0.0011 0.0013 0.0008 0.0008 0.0015 0.0008 0.0013 0.0003 0.0007 0.0007 0.0018 0.0006 0.0009 0.0002 0.0004 0.0002 0.0117 0.0003
Bud Site 0.0018 0.0024 0.0036 0.0009 0.0015 0.0002 0.0042 0.0015 0.0034 0.0004 0.008 0.0002 0.0043 0.0018 0.0009 0.0006 0.0038 0.0001
Cell Periphery 0.0011 0.0011 0.0008 0.0002 0.0007 0.0004 0.0055 0.0002 0.0004 0.0002 0.0002 0.0002 0.0004 0.0002 0.0002 0.0001 0.0003 0.0001
Cytoplasm 0.6561 0.8896 0.7725 0.7223 0.6281 0.7579 0.5945 0.8988 0.7762 0.6673 0.694 0.7513 0.5746 0.8974 0.7764 0.8148 0.5732 0.8093
Cytoplasmic Foci 0.0338 0.0188 0.0206 0.0298 0.0484 0.0235 0.0462 0.0158 0.0317 0.0293 0.0478 0.0255 0.0546 0.0159 0.0291 0.0273 0.0402 0.0235
Eisosomes 0.0003 0 0 0 0.0006 0 0.0005 0 0 0 0.0001 0.0001 0.0007 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0942 0.0257 0.05 0.0242 0.0443 0.0615 0.0773 0.0197 0.034 0.1059 0.0572 0.0784 0.0543 0.0212 0.0514 0.012 0.0122 0.0332
Endosome 0.0867 0.0323 0.0821 0.1194 0.1457 0.0927 0.1178 0.0343 0.088 0.0938 0.0958 0.0864 0.1101 0.0393 0.0852 0.0765 0.2103 0.0844
Golgi 0.0196 0.0071 0.0131 0.0121 0.021 0.0178 0.0368 0.0089 0.0264 0.0287 0.0273 0.0208 0.0323 0.0076 0.0208 0.0075 0.0482 0.0223
Lipid Particles 0.0123 0.0001 0.0004 0.0013 0.0082 0.0001 0.0228 0.0001 0.0012 0.0126 0.0042 0.0008 0.0214 0.001 0.0005 0.0008 0.0029 0.0004
Mitochondria 0.0201 0.008 0.0227 0.007 0.025 0.0298 0.0346 0.0103 0.0148 0.0318 0.0394 0.0173 0.0558 0.0056 0.0125 0.0017 0.0276 0.016
None 0.0097 0.0016 0.0021 0.0009 0.0056 0.0014 0.0025 0.0007 0.001 0.0012 0.0009 0.0017 0.0041 0.001 0.0009 0.0016 0.0012 0.0008
Nuclear Periphery 0.0079 0.0016 0.0033 0.0013 0.0059 0.0013 0.0061 0.0006 0.0026 0.003 0.002 0.0015 0.0177 0.0016 0.0025 0.0014 0.0048 0.0011
Nucleolus 0.0004 0 0 0 0.0008 0 0.0004 0 0.0001 0 0.0002 0 0.0004 0.0001 0 0 0.0005 0
Nucleus 0.0019 0.0008 0.0012 0.0009 0.0026 0.0009 0.0017 0.0006 0.0009 0.0008 0.0011 0.0008 0.0075 0.0008 0.0008 0.0011 0.0044 0.0006
Peroxisomes 0.0112 0.0002 0.0001 0.0591 0.0243 0.0001 0.0102 0.0001 0.0013 0.0051 0.003 0.0004 0.0135 0.0002 0.0018 0.0014 0.0022 0.0001
Punctate Nuclear 0.0064 0.0001 0.0001 0.0001 0.0045 0.0001 0.0022 0.0001 0.0003 0.0003 0.0007 0.0004 0.0033 0.0003 0.0002 0.0017 0.0007 0.0001
Vacuole 0.0143 0.004 0.0176 0.0112 0.0108 0.0052 0.0112 0.0046 0.008 0.0087 0.0043 0.0035 0.0068 0.0023 0.0061 0.007 0.0115 0.0025
Vacuole Periphery 0.0039 0.0017 0.005 0.0045 0.0061 0.0029 0.0033 0.0011 0.0031 0.0038 0.0027 0.0025 0.0039 0.001 0.0027 0.0026 0.0077 0.0022

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 76.3753 54.8674 53.8772 63.7242 75.9882 62.7269 72.5782 72.3519 70.3174 75.0762
Translational Efficiency 1.0616 1.28 1.3316 0.9903 1.058 1.4855 0.9779 1.0566 1.089 1.1015

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; binds to mRNAs under glucose starvation, most often in the 3' UTR; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress; phosphorylation by PKA inhibits P body foci formation
Localization
Cell Percentages cytoplasm (94%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Pat1

Pat1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pat1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available