Standard name | |
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Description | Mismatch repair protein; forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 4 | 5 | 5 | 11 | 8 | 6 | 6 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 5 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 5 | 4 | 11 | 13 | 11 | 17 | 17 | 16 | 7 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 3 | 4 | 5 | 18 | 22 | 30 | 45 | 24 | 39 | 24 | 30 | 23 | 65 | 92 | 20 | 16 | 36 | 0 | 1 | 0 | 0 | 3 | 3 |
Endoplasmic Reticulum | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 10 | 7 | 8 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 2 | 4 | 3 | 0 | 0 | 0 | 3 | 6 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 1 | 1 | 0 | 1 |
Mitochondria | 9 | 35 | 113 | 161 | 157 | 333 | 439 | 481 | 455 | 525 | 359 | 517 | 10 | 14 | 48 | 21 | 10 | 29 | 1 | 1 | 0 | 1 | 3 | 12 |
Nucleus | 180 | 111 | 182 | 248 | 334 | 360 | 427 | 503 | 335 | 351 | 244 | 262 | 264 | 322 | 344 | 280 | 183 | 253 | 176 | 111 | 184 | 159 | 299 | 218 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 3 | 1 | 4 | 1 | 1 | 1 | 0 | 0 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 2 | 2 | 0 | 4 | 3 | 4 | 2 | 3 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 18 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 1 | 1 | 2 | 2 | 5 | 3 | 5 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 0 | 4 | 2 |
Vac/Vac Membrane | 6 | 9 | 1 | 2 | 10 | 9 | 14 | 18 | 10 | 4 | 8 | 11 | 2 | 8 | 14 | 28 | 25 | 37 | 11 | 9 | 25 | 17 | 67 | 98 |
Unique Cell Count | 195 | 130 | 212 | 287 | 409 | 483 | 643 | 764 | 553 | 624 | 425 | 573 | 292 | 392 | 460 | 323 | 219 | 326 | 200 | 128 | 214 | 185 | 403 | 371 |
Labelled Cell Count | 206 | 158 | 302 | 424 | 529 | 743 | 937 | 1074 | 860 | 955 | 663 | 839 | 301 | 414 | 502 | 366 | 244 | 376 | 200 | 128 | 214 | 185 | 403 | 371 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 5.8 | 4.3 | 3.6 | 3.5 | 3.7 | 3.3 | 3.2 | 3.2 | 2.8 | 2.8 | 2.8 | 2.5 | 5.2 | 5.2 | 4.5 | 6.3 | 6.7 | 7.0 | 5.3 | 4.7 | 5.4 |
Std Deviation (1e-4) | 1.1 | 0.6 | 0.7 | 0.7 | 0.6 | 0.7 | 0.8 | 0.7 | 0.7 | 0.6 | 0.7 | 0.6 | 1.1 | 1.3 | 1.2 | 1.3 | 1.6 | 1.9 | 1.0 | 1.0 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.03 | -0.14 | -0.18 | -0.16 | -0.4 | -0.4 | -0.4 | -0.53 | 0.52 | 0.52 | 0.3 | 0.8 | 0.89 | 0.95 | 0.53 | 0.37 | 0.59 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 4.1 | 4.8 | 4.8 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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