Standard name
Description Mismatch repair protein; forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0.05 0.06 0 0.06 0.06 0.05 0.08 0.17 0.2 0.06 0.07 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.27 0.53 0.56 0.38 0.69 0.68 0.63 0.82 0.84 0.84 0.9 0 0 0.1 0.07 0.05 0.09 0 0 0 0 0 0
Nucleus 0.92 0.85 0.86 0.86 0.82 0.75 0.66 0.66 0.61 0.56 0.57 0.46 0.9 0.82 0.75 0.87 0.84 0.78 0.88 0.87 0.86 0.86 0.74 0.59
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.11 0.11 0.06 0.08 0.12 0.09 0.17 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0
Bud 0 0 0 0 1 4 5 5 11 8 6 6 0 0 1 0 0 0 1 0 0 0 5 1
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 5 4 11 13 11 17 17 16 7 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0
Cytoplasm 7 3 4 5 18 22 30 45 24 39 24 30 23 65 92 20 16 36 0 1 0 0 3 3
Endoplasmic Reticulum 2 0 1 0 0 0 0 0 0 0 0 0 0 2 0 10 7 8 0 0 0 0 0 1
Endosome 1 0 0 0 1 0 0 0 0 0 0 0 1 0 1 2 2 4 3 0 0 0 3 6
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 1 1 1 0 1
Mitochondria 9 35 113 161 157 333 439 481 455 525 359 517 10 14 48 21 10 29 1 1 0 1 3 12
Nucleus 180 111 182 248 334 360 427 503 335 351 244 262 264 322 344 280 183 253 176 111 184 159 299 218
Nuclear Periphery 0 0 0 0 1 2 2 3 1 4 1 1 1 0 0 4 1 1 0 0 0 0 0 1
Nucleolus 0 0 0 2 2 0 4 3 4 2 3 2 0 2 0 0 0 0 0 0 0 0 11 18
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 1 2 2 5 3 5 2 2 0 0 0 0 0 5 0 0 0 0 4 2
Vac/Vac Membrane 6 9 1 2 10 9 14 18 10 4 8 11 2 8 14 28 25 37 11 9 25 17 67 98
Unique Cell Count 195 130 212 287 409 483 643 764 553 624 425 573 292 392 460 323 219 326 200 128 214 185 403 371
Labelled Cell Count 206 158 302 424 529 743 937 1074 860 955 663 839 301 414 502 366 244 376 200 128 214 185 403 371


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 4.3 3.6 3.5 3.7 3.3 3.2 3.2 2.8 2.8 2.8 2.5 5.2 5.2 4.5 6.3 6.7 7.0 5.3 4.7 5.4
Std Deviation (1e-4) 1.1 0.6 0.7 0.7 0.6 0.7 0.8 0.7 0.7 0.6 0.7 0.6 1.1 1.3 1.2 1.3 1.6 1.9 1.0 1.0 1.3
Intensity Change (Log2) – – – -0.05 0.03 -0.14 -0.18 -0.16 -0.4 -0.4 -0.4 -0.53 0.52 0.52 0.3 0.8 0.89 0.95 0.53 0.37 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.1 4.8 4.8


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Msh3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available