Standard name
Description Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0.24 0.13 0 0 0 0 0 0 0 0 0 0.18 0.09 0 0.15 0.12 0.11 0.13 0.17 0.21 0.13 0.14 0.16
Bud 0 0.05 0.05 0.07 0.1 0.06 0.11 0.09 0.07 0.09 0.1 0.11 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.07 0 0.05 0 0 0 0 0 0 0 0 0.07 0.09 0.09 0.09 0.12 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0.07 0.22 0.08 0 0 0 0 0 0 0 0 0 0.18 0.07 0 0.05 0.05 0.05 0 0 0 0 0 0
Endosome 0.06 0.05 0.14 0.13 0.15 0 0 0.08 0 0 0 0 0.23 0.22 0.38 0.26 0.28 0.34 0.22 0.17 0.15 0.16 0.13 0.15
Golgi 0.12 0.3 0.21 0 0 0 0 0 0 0 0 0 0.35 0.42 0.16 0.3 0.29 0.19 0.47 0.47 0.45 0.42 0.46 0.49
Mitochondria 0.79 0.56 0.62 0.78 0.77 0.92 0.93 0.83 0.92 0.91 0.96 0.9 0.19 0.12 0.27 0.11 0.13 0.11 0.06 0.05 0.05 0.13 0.12 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0.1 0.15 0.05 0 0.08 0 0 0 0.06 0 0.05 0.22 0 0 0 0.05 0 0.09 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0.09 0 0.09 0.1 0.17 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0.07 0.07 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 19 49 49 8 18 10 18 6 3 6 3 4 48 24 9 53 33 23 39 44 99 49 51 69
Bud 9 10 17 21 41 29 72 55 27 35 35 49 7 6 6 18 8 9 1 3 1 3 6 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1
Bud Site 0 0 1 1 2 13 14 11 10 23 10 11 0 0 0 0 1 1
Cell Periphery 1 1 5 3 1 2 8 6 3 2 1 9 2 2 1 5 3 5 0 0 0 0 0 0
Cytoplasm 9 15 9 17 15 14 13 12 8 4 5 5 18 23 27 32 34 30 1 0 0 4 2 4
Endoplasmic Reticulum 19 44 28 7 9 15 11 8 4 4 5 9 48 17 5 17 15 10 6 6 5 11 10 9
Endosome 16 10 51 40 63 10 11 54 15 9 1 18 61 57 115 92 80 74 67 43 70 61 50 65
Golgi 32 62 78 11 17 0 1 3 0 1 2 3 93 108 47 106 83 41 142 119 208 160 173 210
Mitochondria 203 115 228 246 323 440 613 534 377 344 351 394 50 30 81 40 37 23 18 13 23 50 46 30
Nucleus 0 0 0 0 2 1 4 2 3 1 1 3 1 1 0 8 6 2 0 0 1 0 0 2
Nuclear Periphery 2 0 1 1 0 4 4 6 5 2 5 4 1 0 0 3 1 2 1 0 1 2 1 1
Nucleolus 5 2 1 5 6 10 25 26 12 14 14 15 0 0 4 5 2 3 0 0 0 0 0 0
Peroxisomes 2 2 19 33 64 23 16 51 15 16 8 25 11 14 65 1 12 2 15 7 41 12 14 13
SpindlePole 4 1 12 12 8 5 20 29 13 15 5 7 14 24 8 32 28 37 3 2 2 2 3 1
Vac/Vac Membrane 6 3 4 5 8 2 3 18 10 7 5 13 12 17 27 25 20 12 7 10 3 16 12 10
Unique Cell Count 256 204 365 315 419 478 662 640 408 380 365 438 267 257 299 350 285 217 306 255 465 383 379 429
Labelled Cell Count 327 314 503 410 577 578 833 821 505 483 451 569 366 323 395 437 363 274 306 255 465 383 379 429


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 8.6 7.4 6.9 6.7 5.9 5.7 5.8 5.5 5.4 5.0 5.6 9.1 9.0 8.5 8.7 8.4 7.7 9.1 9.3 9.8
Std Deviation (1e-4) 1.0 1.6 1.0 1.5 1.4 1.3 1.4 1.3 1.7 1.1 1.4 3.6 1.5 1.4 1.5 1.7 1.7 1.5 1.5 1.5 1.6
Intensity Change (Log2) -0.1 -0.14 -0.32 -0.38 -0.35 -0.43 -0.45 -0.55 -0.39 0.3 0.28 0.2 0.24 0.19 0.06 0.3 0.33 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -5.1 -4.6 -6.4 -6.6 -8.4 -7.0 -6.2 -6.6 -7.1 1.6 -1.6 -4.7 0.7 -0.7 -1.0
Bud 1.1 2.7 0.9 3.4 2.3 1.2 2.4 2.6 3.4 -1.3 -1.5 -1.9 0.3 -1.2 -0.3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 2.8 2.4 0 2.6 4.5 2.7 2.6 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.7 0 0 0 0 0 0
Cytoplasm 2.0 0.9 0.4 -0.5 -0.6 -0.5 -1.5 -1.1 -1.4 2.6 3.6 3.7 3.8 4.8 5.3
Endoplasmic Reticulum -3.2 -3.6 -3.0 -4.8 -5.3 -4.7 -4.5 -4.1 -3.8 3.9 -0.5 -3.5 -1.5 -1.2 -1.4
Endosome -0.5 0.4 -6.6 -7.9 -2.8 -5.1 -5.8 -7.2 -5.0 2.9 2.7 7.3 4.1 4.4 5.7
Golgi -6.9 -7.4 -10.6 -12.2 -11.7 -9.8 -9.4 -9.0 -9.7 3.8 5.5 -1.9 2.7 2.3 -0.7
Mitochondria 4.4 4.5 10.5 12.0 7.5 10.1 9.1 11.2 9.3 -10.9 -12.7 -9.1 -14.1 -12.7 -12.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 3.4 3.6 2.9 3.3 3.4 3.2 0 0 0 0 0 0
Peroxisomes 2.6 4.6 -0.3 -2.4 1.7 -1.0 -0.6 -2.2 0.3 -0.6 0.1 6.4 -4.0 -0.6 -2.7
SpindlePole 0.4 -1.2 -2.3 -0.2 1.0 -0.1 0.5 -1.7 -1.6 1.2 3.2 -0.5 3.3 3.4 5.8
Vacuole 0 0.9 0 0 1.8 1.4 0.8 0 1.8 2.7 3.8 4.8 4.1 4.0 3.2


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc50-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available