Standard name | |
---|---|
Human Ortholog | |
Description | Conserved phosphatase acting as a metabolite repair enzyme; shows specific dephosphorylating activity on two side-products of central carbohydrate metabolism, 2-phosphoglycolate and 4-phosphoerythronate; alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity; human ortholog PGP shows similar substrate specificity, deletion causes similar metabolite accumulation phenotypes, suggesting conserved role in eliminating glycolytic byproducts |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 2 | 5 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 4 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 1 | 7 | 8 | 12 | 4 | 8 | 15 | 17 | 3 | 16 | 3 | 2 | 5 | 2 | 9 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 245 | 168 | 138 | 286 | 373 | 343 | 392 | 322 | 229 | 294 | 186 | 297 | 338 | 496 | 517 | 69 | 181 | 140 | 238 | 159 | 145 | 124 | 396 | 335 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2 | 4 | 13 | 25 | 27 | 10 | 24 | 6 | 26 | 2 | 2 | 3 | 2 | 7 | 3 | 0 | 0 | 1 | 0 | 1 | 3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 3 | 12 | 5 | 15 | 5 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 1 | 2 | 2 | 3 | 7 | 3 | 5 | 10 | 4 | 4 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 3 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 5 | 11 | 15 | 21 | 7 | 21 | 19 | 42 | 1 | 0 | 6 | 3 | 6 | 0 | 0 | 3 | 1 | 0 | 0 | 1 |
Unique Cell Count | 246 | 168 | 139 | 289 | 382 | 368 | 437 | 372 | 272 | 355 | 225 | 378 | 340 | 498 | 523 | 71 | 188 | 145 | 240 | 166 | 157 | 131 | 402 | 349 |
Labelled Cell Count | 247 | 170 | 140 | 299 | 392 | 385 | 449 | 386 | 285 | 377 | 234 | 395 | 345 | 500 | 531 | 77 | 204 | 152 | 240 | 166 | 157 | 131 | 402 | 349 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 18.5 | 26.8 | 21.7 | 20.8 | 21.2 | 18.0 | 15.9 | 16.4 | 14.2 | 14.6 | 13.3 | 14.2 | 23.1 | 23.5 | 22.1 | 33.5 | 32.5 | 31.6 | 20.9 | 23.6 | 24.0 |
Std Deviation (1e-4) | 3.5 | 4.7 | 4.4 | 3.8 | 3.7 | 3.3 | 3.2 | 3.3 | 3.0 | 2.8 | 2.9 | 3.0 | 4.6 | 4.1 | 4.2 | 8.0 | 7.5 | 9.6 | 4.8 | 4.0 | 4.8 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.03 | -0.27 | -0.45 | -0.4 | -0.61 | -0.57 | -0.71 | -0.61 | 0.1 | 0.12 | 0.03 | 0.63 | 0.58 | 0.54 | -0.05 | 0.12 | 0.15 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 2.4 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.3 | -1.2 | -2.8 | -3.6 | -4.3 | -4.7 | -5.0 | -4.9 | -5.7 | 0.2 | 0.5 | -0.4 | -1.2 | -1.7 | -1.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2.9 | 3.3 | 0 | 3.1 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 2.9 | 0 | 2.9 | 3.5 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 12.6238 | 14.9206 | 13.612 | 11.4812 | 11.7327 | 14.0985 | 13.7159 | 18.0983 | 15.8797 | 17.0315 | 12.9297 | 16.4828 | 14.0586 | 18.7526 | 15.4526 | 17.3463 | 9.8577 | 15.3939 |
Actin | 0.0091 | 0.0004 | 0.0035 | 0.0044 | 0.0091 | 0.0003 | 0.0082 | 0.0015 | 0.01 | 0.0002 | 0.0091 | 0.0044 | 0.0132 | 0.0011 | 0.0127 | 0.012 | 0.029 | 0.0008 |
Bud | 0.0005 | 0.0006 | 0.0003 | 0.0013 | 0.0003 | 0.0002 | 0.0005 | 0.0031 | 0.0009 | 0.0001 | 0.0016 | 0.0005 | 0.0007 | 0.0018 | 0.0012 | 0.0001 | 0.0003 | 0.0003 |
Bud Neck | 0.0006 | 0.0002 | 0.0005 | 0.0005 | 0.0007 | 0.0009 | 0.0013 | 0.0002 | 0.0005 | 0.0004 | 0.0017 | 0.0011 | 0.001 | 0.0001 | 0.0006 | 0.0003 | 0.0007 | 0.0009 |
Bud Periphery | 0.0013 | 0.0001 | 0.0002 | 0.0008 | 0.0002 | 0.0001 | 0.0004 | 0.0005 | 0.0005 | 0 | 0.0022 | 0.0002 | 0.0006 | 0.0003 | 0.0018 | 0.0001 | 0.0004 | 0.0009 |
Bud Site | 0.0004 | 0.0012 | 0.0006 | 0.0007 | 0.0004 | 0 | 0.0014 | 0.0007 | 0.0007 | 0 | 0.0022 | 0.0002 | 0.0036 | 0.0014 | 0.0064 | 0.0002 | 0.0016 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.8959 | 0.9774 | 0.9394 | 0.897 | 0.8292 | 0.9583 | 0.8701 | 0.9806 | 0.9316 | 0.9802 | 0.749 | 0.9373 | 0.896 | 0.9866 | 0.908 | 0.9375 | 0.7598 | 0.9554 |
Cytoplasmic Foci | 0.0089 | 0.0016 | 0.0068 | 0.0099 | 0.0145 | 0.0016 | 0.0186 | 0.0011 | 0.0059 | 0.0005 | 0.0081 | 0.0037 | 0.0141 | 0.0014 | 0.0133 | 0.0045 | 0.046 | 0.0012 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0004 | 0 |
Endoplasmic Reticulum | 0.0061 | 0.0022 | 0.0039 | 0.0079 | 0.0102 | 0.002 | 0.0064 | 0.0005 | 0.0047 | 0.0007 | 0.0108 | 0.0024 | 0.0058 | 0.0003 | 0.0022 | 0.0006 | 0.0056 | 0.0012 |
Endosome | 0.0154 | 0.0005 | 0.0073 | 0.012 | 0.0349 | 0.0013 | 0.0181 | 0.0002 | 0.0043 | 0.0002 | 0.0383 | 0.0059 | 0.0142 | 0.0002 | 0.0062 | 0.005 | 0.0261 | 0.0029 |
Golgi | 0.0022 | 0 | 0.0038 | 0.0018 | 0.0114 | 0.0001 | 0.0029 | 0 | 0.0013 | 0 | 0.0076 | 0.0017 | 0.0034 | 0 | 0.0023 | 0.0048 | 0.0141 | 0.0009 |
Lipid Particles | 0.0015 | 0 | 0.0015 | 0.001 | 0.0258 | 0 | 0.007 | 0 | 0.0031 | 0 | 0.0383 | 0.0026 | 0.0054 | 0 | 0.0038 | 0.0169 | 0.0609 | 0.0008 |
Mitochondria | 0.0049 | 0.0001 | 0.0011 | 0.0053 | 0.0056 | 0.0019 | 0.0039 | 0.0003 | 0.0024 | 0.0001 | 0.0171 | 0.0019 | 0.0071 | 0.0001 | 0.0133 | 0.0018 | 0.0133 | 0.0186 |
None | 0.0046 | 0.0012 | 0.0011 | 0.003 | 0.0031 | 0.0008 | 0.006 | 0.0008 | 0.0027 | 0.0004 | 0.0046 | 0.0022 | 0.0025 | 0.0006 | 0.0012 | 0.0011 | 0.0043 | 0.0005 |
Nuclear Periphery | 0.0088 | 0.0029 | 0.0042 | 0.013 | 0.0176 | 0.0043 | 0.0077 | 0.0015 | 0.0081 | 0.0022 | 0.0315 | 0.0056 | 0.0075 | 0.0008 | 0.0064 | 0.0015 | 0.0054 | 0.0022 |
Nucleolus | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0 | 0.0001 | 0 | 0.0032 | 0.001 | 0.0002 | 0 | 0.0005 | 0 | 0.0003 | 0.0001 |
Nucleus | 0.0253 | 0.0105 | 0.0211 | 0.032 | 0.0177 | 0.0267 | 0.0263 | 0.0083 | 0.0181 | 0.0144 | 0.0334 | 0.0237 | 0.013 | 0.0046 | 0.0146 | 0.0061 | 0.0076 | 0.0083 |
Peroxisomes | 0.0012 | 0 | 0.0012 | 0.0009 | 0.0086 | 0 | 0.0051 | 0.0001 | 0.0018 | 0 | 0.017 | 0.0004 | 0.0046 | 0 | 0.0023 | 0.0058 | 0.0153 | 0.0001 |
Punctate Nuclear | 0.0071 | 0.0005 | 0.0006 | 0.0037 | 0.0053 | 0.0004 | 0.01 | 0.0003 | 0.0016 | 0.0002 | 0.0126 | 0.0025 | 0.0043 | 0.0002 | 0.0015 | 0.0006 | 0.0061 | 0.0004 |
Vacuole | 0.0034 | 0.0004 | 0.0019 | 0.0031 | 0.0027 | 0.0008 | 0.0039 | 0.0002 | 0.0011 | 0.0003 | 0.0072 | 0.0016 | 0.0017 | 0.0002 | 0.0007 | 0.0004 | 0.0015 | 0.0016 |
Vacuole Periphery | 0.002 | 0.0001 | 0.0004 | 0.0014 | 0.002 | 0.0003 | 0.0013 | 0 | 0.0005 | 0.0001 | 0.004 | 0.001 | 0.0008 | 0 | 0.0005 | 0.0003 | 0.0013 | 0.0027 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 59.3418 | 56.7245 | 48.3279 | 58.7503 | 68.5597 | 44.6285 | 52.3091 | 56.2731 | 60.432 | 61.5363 |
Translational Efficiency | 2.2263 | 1.9328 | 2.2231 | 1.9289 | 1.466 | 2.9166 | 2.4905 | 1.9176 | 1.9843 | 2.0775 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1663 | 605 | 2128 | 1448 | 1359 | 838 | 142 | 1024 | 3022 | 1443 | 2270 | 2472 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1750.64 | 1928.71 | 1933.46 | 2212.42 | 1622.74 | 1829.96 | 2245.10 | 2278.84 | 1693.12 | 1871.36 | 1952.95 | 2239.93 |
Standard Deviation | 313.39 | 355.64 | 388.93 | 515.77 | 302.76 | 312.44 | 430.40 | 555.26 | 315.14 | 334.80 | 398.86 | 533.49 |
Intensity Change Log 2 | — | 0.139754 | 0.143302 | 0.337743 | — | 0.173380 | 0.468348 | 0.489868 | — | 0.156027 | 0.308811 | 0.412925 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000164 | 0.000213 | 0.000191 | 0.001014 | 0.000433 | 0.000254 | 0.000835 | 0.000330 | 0.000285 | 0.000237 | 0.000231 | 0.000730 |
Bud Neck | 0.000300 | 0.000593 | 0.000586 | 0.003465 | 0.000397 | 0.000759 | 0.008648 | 0.002233 | 0.000344 | 0.000690 | 0.001090 | 0.002955 |
Bud Site | 0.002048 | 0.001677 | 0.001900 | 0.017001 | 0.002310 | 0.005516 | 0.006394 | 0.011166 | 0.002166 | 0.003906 | 0.002181 | 0.014584 |
Cell Periphery | 0.000104 | 0.000144 | 0.000100 | 0.000282 | 0.000113 | 0.000157 | 0.000643 | 0.000235 | 0.000108 | 0.000152 | 0.000134 | 0.000262 |
Cytoplasm | 0.888934* | 0.836395* | 0.738739* | 0.685653* | 0.866946* | 0.846241* | 0.569464* | 0.736471* | 0.879046* | 0.842113* | 0.728150* | 0.706704* |
Cytoplasmic Foci | 0.009497 | 0.008713 | 0.003868 | 0.008727 | 0.007739 | 0.018378 | 0.018394 | 0.012386 | 0.008707 | 0.014326 | 0.004776 | 0.010243 |
Eisosomes | 0.000001 | 0.000001 | 0.000001 | 0.000008 | 0.000001 | 0.000002 | 0.000015 | 0.000005 | 0.000001 | 0.000002 | 0.000002 | 0.000007 |
Endoplasmic Reticulum | 0.000248 | 0.000389 | 0.000051 | 0.000674 | 0.000537 | 0.000366 | 0.001720 | 0.000189 | 0.000378 | 0.000376 | 0.000156 | 0.000473 |
Endosome | 0.000248 | 0.000554 | 0.000066 | 0.010563 | 0.000358 | 0.001627 | 0.003819 | 0.008146 | 0.000297 | 0.001177 | 0.000301 | 0.009562 |
Golgi | 0.000709 | 0.001085 | 0.000133 | 0.009348 | 0.001361 | 0.002896 | 0.001379 | 0.010488 | 0.001002 | 0.002137 | 0.000211 | 0.009820 |
Lipid Particles | 0.000387 | 0.000512 | 0.000388 | 0.001250 | 0.000447 | 0.000557 | 0.003683 | 0.001497 | 0.000414 | 0.000539 | 0.000594 | 0.001353 |
Mitochondria | 0.000076 | 0.000105 | 0.000132 | 0.005975 | 0.000095 | 0.000124 | 0.000261 | 0.006559 | 0.000085 | 0.000116 | 0.000140 | 0.006217 |
Mitotic Spindle | 0.001585 | 0.002331 | 0.002457 | 0.049513 | 0.001624 | 0.005870 | 0.011480 | 0.020148 | 0.001602 | 0.004386 | 0.003021 | 0.037349 |
None | 0.007976 | 0.011386 | 0.009727 | 0.005992 | 0.007009 | 0.007389 | 0.003615 | 0.007908 | 0.007541 | 0.009064 | 0.009345 | 0.006785 |
Nuclear Periphery | 0.000086 | 0.000086 | 0.000110 | 0.000578 | 0.000138 | 0.000501 | 0.001545 | 0.000235 | 0.000109 | 0.000327 | 0.000200 | 0.000436 |
Nuclear Periphery Foci | 0.003979 | 0.003185 | 0.000656 | 0.005124 | 0.003518 | 0.005241 | 0.010710 | 0.002088 | 0.003772 | 0.004379 | 0.001285 | 0.003867 |
Nucleolus | 0.000053 | 0.000115 | 0.000076 | 0.000404 | 0.000089 | 0.000132 | 0.000407 | 0.000113 | 0.000069 | 0.000125 | 0.000097 | 0.000283 |
Nucleus | 0.076826 | 0.124180 | 0.234425* | 0.134188* | 0.099019 | 0.088213 | 0.217230* | 0.150265* | 0.086806 | 0.103293 | 0.233350* | 0.140848* |
Peroxisomes | 0.000077 | 0.000031 | 0.000033 | 0.000397 | 0.000025 | 0.000072 | 0.000166 | 0.000290 | 0.000054 | 0.000055 | 0.000041 | 0.000353 |
Vacuole | 0.005574 | 0.007768 | 0.006319 | 0.043325 | 0.005856 | 0.013660 | 0.138554 | 0.024136 | 0.005700 | 0.011190 | 0.014591 | 0.035376 |
Vacuole Periphery | 0.001128 | 0.000535 | 0.000041 | 0.016520 | 0.001984 | 0.002046 | 0.001040 | 0.005110 | 0.001513 | 0.001413 | 0.000103 | 0.011793 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.28 | -1.63 | -2.55 | -1.51 | -0.93 | 0.65 | -3.05 | 0.45 | -0.65 | 5.71 | -0.15 | -0.70 | -1.22 | -1.56 | -0.39 |
Bud Neck | -4.07 | -8.87 | -11.44 | -8.05 | -7.57 | -5.35 | -7.04 | -9.62 | -6.76 | 5.51 | -6.96 | -9.86 | -14.92 | -10.41 | -5.02 |
Bud Site | -0.02 | -0.82 | -5.34 | -5.54 | -5.08 | -2.75 | -6.31 | -5.52 | -2.97 | 0.14 | -2.77 | -1.74 | -7.63 | -4.95 | -6.82 |
Cell Periphery | -2.98 | -4.34 | -6.10 | -2.15 | -2.60 | -3.48 | -7.42 | -9.70 | -5.59 | 5.67 | -4.68 | -7.39 | -10.77 | -4.98 | -2.18 |
Cytoplasm | 9.26 | 36.48 | 32.09 | 17.07 | 3.48 | 4.92 | 18.64 | 21.56 | 14.28 | -10.54 | 10.01 | 38.64 | 38.12 | 22.32 | 0.40 |
Cytoplasmic Foci | -0.20 | 3.71 | 0.35 | 0.53 | -4.01 | -7.24 | -5.65 | -7.23 | 0.39 | 2.14 | -6.32 | 1.66 | -5.36 | 2.02 | -7.01 |
Eisosomes | -2.01 | -3.76 | -7.88 | -5.27 | -6.25 | -4.08 | -7.71 | -8.21 | -4.06 | 6.01 | -4.69 | -7.16 | -11.16 | -6.16 | -5.09 |
Endoplasmic Reticulum | -0.86 | 4.77 | 1.25 | 1.23 | -2.88 | 1.53 | -2.64 | 4.47 | 4.34 | 4.04 | 0.03 | 4.01 | 4.33 | 2.80 | 0.52 |
Endosome | -1.82 | 4.81 | -3.70 | -2.95 | -3.97 | -2.88 | -6.54 | -2.41 | -0.67 | 1.45 | -3.38 | 0.19 | -4.36 | -2.17 | -4.38 |
Golgi | -0.80 | 5.93 | -1.72 | -1.07 | -2.49 | -1.05 | 0.23 | -1.13 | 0.02 | -1.46 | -1.41 | 3.19 | -1.95 | -0.32 | -3.56 |
Lipid Particles | -2.10 | -2.40 | -8.65 | -6.36 | -3.78 | -2.25 | -7.71 | -6.94 | -6.06 | 3.67 | -3.28 | -3.89 | -10.41 | -8.45 | -4.43 |
Mitochondria | -0.97 | -6.27 | -2.14 | -2.08 | -1.98 | -0.76 | -4.92 | -1.91 | -1.88 | -1.65 | -1.35 | -7.73 | -2.87 | -2.80 | -2.67 |
Mitotic Spindle | -0.91 | -2.70 | -6.14 | -5.67 | -5.22 | -2.67 | -2.11 | -4.02 | -1.36 | -0.18 | -2.88 | -3.16 | -7.28 | -5.02 | -5.61 |
None | -1.75 | -1.10 | 3.97 | 3.82 | 9.97 | 0.29 | 5.99 | 2.12 | 1.44 | -5.92 | -1.02 | -1.65 | 4.48 | 4.04 | 9.74 |
Nuclear Periphery | -1.06 | -5.62 | -10.70 | -11.20 | -8.47 | -1.32 | -6.17 | -5.30 | 0.42 | 5.27 | -1.44 | -7.16 | -11.47 | -0.84 | -4.88 |
Nuclear Periphery Foci | 0.26 | 8.17 | 2.43 | 1.95 | -3.16 | -2.29 | -4.72 | 2.65 | 3.41 | 5.38 | -1.92 | 7.64 | 3.29 | 3.79 | -2.81 |
Nucleolus | -1.27 | -8.99 | -5.74 | -0.34 | -4.59 | -1.10 | -4.77 | -1.12 | 0.55 | 4.69 | -1.72 | -3.41 | -5.50 | -0.51 | -4.13 |
Nucleus | -9.58 | -39.67 | -18.09 | -4.38 | 18.10 | 1.27 | -8.56 | -9.27 | -9.71 | 5.11 | -6.06 | -39.49 | -19.45 | -10.75 | 21.16 |
Peroxisomes | 0.98 | 0.76 | -1.12 | -3.33 | -2.76 | -2.76 | -7.64 | -3.74 | -2.13 | 0.79 | -0.07 | 0.11 | -2.68 | -3.56 | -3.34 |
Vacuole | -3.28 | -9.81 | -24.61 | -20.49 | -21.40 | -6.20 | -10.62 | -18.48 | -13.19 | 6.24 | -7.06 | -12.37 | -30.72 | -23.36 | -19.66 |
Vacuole Periphery | 0.81 | 1.70 | -2.03 | -2.97 | -3.80 | 0.17 | 1.75 | 0.89 | 0.97 | -1.35 | 0.38 | 2.86 | -1.22 | -1.80 | -4.40 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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