Standard name
Human Ortholog
Description Conserved phosphatase acting as a metabolite repair enzyme; shows specific dephosphorylating activity on two side-products of central carbohydrate metabolism, 2-phosphoglycolate and 4-phosphoerythronate; alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity; human ortholog PGP shows similar substrate specificity, deletion causes similar metabolite accumulation phenotypes, suggesting conserved role in eliminating glycolytic byproducts

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 1.0 1.0 0.99 0.99 0.98 0.93 0.9 0.87 0.84 0.83 0.83 0.79 0.99 1.0 0.99 0.97 0.96 0.97 0.99 0.96 0.93 0.95 0.99 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0.07 0 0.07 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 0.06 0.08 0.11 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 1 1 2 2 5 1 2 0 0 0 0 0 1 0 0 0 0 0 0
Bud 0 0 0 0 0 2 1 0 4 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 2 1 7 8 12 4 8 15 17 3 16 3 2 5 2 9 5 0 0 0 0 0 0
Cytoplasm 245 168 138 286 373 343 392 322 229 294 186 297 338 496 517 69 181 140 238 159 145 124 396 335
Endoplasmic Reticulum 0 0 0 2 4 13 25 27 10 24 6 26 2 2 3 2 7 3 0 0 1 0 1 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Mitochondria 1 0 0 0 0 0 4 3 12 5 15 5 0 0 0 1 0 1 0 0 1 0 0 0
Nucleus 0 0 1 2 2 3 7 3 5 10 4 4 1 0 0 0 1 1 0 2 1 3 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 5 11 15 21 7 21 19 42 1 0 6 3 6 0 0 3 1 0 0 1
Unique Cell Count 246 168 139 289 382 368 437 372 272 355 225 378 340 498 523 71 188 145 240 166 157 131 402 349
Labelled Cell Count 247 170 140 299 392 385 449 386 285 377 234 395 345 500 531 77 204 152 240 166 157 131 402 349


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.5 26.8 21.7 20.8 21.2 18.0 15.9 16.4 14.2 14.6 13.3 14.2 23.1 23.5 22.1 33.5 32.5 31.6 20.9 23.6 24.0
Std Deviation (1e-4) 3.5 4.7 4.4 3.8 3.7 3.3 3.2 3.3 3.0 2.8 2.9 3.0 4.6 4.1 4.2 8.0 7.5 9.6 4.8 4.0 4.8
Intensity Change (Log2) -0.06 -0.03 -0.27 -0.45 -0.4 -0.61 -0.57 -0.71 -0.61 0.1 0.12 0.03 0.63 0.58 0.54 -0.05 0.12 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 2.4 2.2 0 0 0 0 0 0 0 0
Cytoplasm -0.3 -1.2 -2.8 -3.6 -4.3 -4.7 -5.0 -4.9 -5.7 0.2 0.5 -0.4 -1.2 -1.7 -1.6
Endoplasmic Reticulum 0 0 0 2.9 3.3 0 3.1 0 3.2 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 3.1 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.9 0 2.9 3.5 4.1 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.6238 14.9206 13.612 11.4812 11.7327 14.0985 13.7159 18.0983 15.8797 17.0315 12.9297 16.4828 14.0586 18.7526 15.4526 17.3463 9.8577 15.3939
Actin 0.0091 0.0004 0.0035 0.0044 0.0091 0.0003 0.0082 0.0015 0.01 0.0002 0.0091 0.0044 0.0132 0.0011 0.0127 0.012 0.029 0.0008
Bud 0.0005 0.0006 0.0003 0.0013 0.0003 0.0002 0.0005 0.0031 0.0009 0.0001 0.0016 0.0005 0.0007 0.0018 0.0012 0.0001 0.0003 0.0003
Bud Neck 0.0006 0.0002 0.0005 0.0005 0.0007 0.0009 0.0013 0.0002 0.0005 0.0004 0.0017 0.0011 0.001 0.0001 0.0006 0.0003 0.0007 0.0009
Bud Periphery 0.0013 0.0001 0.0002 0.0008 0.0002 0.0001 0.0004 0.0005 0.0005 0 0.0022 0.0002 0.0006 0.0003 0.0018 0.0001 0.0004 0.0009
Bud Site 0.0004 0.0012 0.0006 0.0007 0.0004 0 0.0014 0.0007 0.0007 0 0.0022 0.0002 0.0036 0.0014 0.0064 0.0002 0.0016 0.0001
Cell Periphery 0.0001 0 0.0002 0.0001 0.0001 0 0.0002 0 0.0001 0 0.0003 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001
Cytoplasm 0.8959 0.9774 0.9394 0.897 0.8292 0.9583 0.8701 0.9806 0.9316 0.9802 0.749 0.9373 0.896 0.9866 0.908 0.9375 0.7598 0.9554
Cytoplasmic Foci 0.0089 0.0016 0.0068 0.0099 0.0145 0.0016 0.0186 0.0011 0.0059 0.0005 0.0081 0.0037 0.0141 0.0014 0.0133 0.0045 0.046 0.0012
Eisosomes 0.0001 0 0.0001 0 0.0002 0 0.0001 0 0.0001 0 0.0002 0.0001 0.0001 0 0.0002 0.0001 0.0004 0
Endoplasmic Reticulum 0.0061 0.0022 0.0039 0.0079 0.0102 0.002 0.0064 0.0005 0.0047 0.0007 0.0108 0.0024 0.0058 0.0003 0.0022 0.0006 0.0056 0.0012
Endosome 0.0154 0.0005 0.0073 0.012 0.0349 0.0013 0.0181 0.0002 0.0043 0.0002 0.0383 0.0059 0.0142 0.0002 0.0062 0.005 0.0261 0.0029
Golgi 0.0022 0 0.0038 0.0018 0.0114 0.0001 0.0029 0 0.0013 0 0.0076 0.0017 0.0034 0 0.0023 0.0048 0.0141 0.0009
Lipid Particles 0.0015 0 0.0015 0.001 0.0258 0 0.007 0 0.0031 0 0.0383 0.0026 0.0054 0 0.0038 0.0169 0.0609 0.0008
Mitochondria 0.0049 0.0001 0.0011 0.0053 0.0056 0.0019 0.0039 0.0003 0.0024 0.0001 0.0171 0.0019 0.0071 0.0001 0.0133 0.0018 0.0133 0.0186
None 0.0046 0.0012 0.0011 0.003 0.0031 0.0008 0.006 0.0008 0.0027 0.0004 0.0046 0.0022 0.0025 0.0006 0.0012 0.0011 0.0043 0.0005
Nuclear Periphery 0.0088 0.0029 0.0042 0.013 0.0176 0.0043 0.0077 0.0015 0.0081 0.0022 0.0315 0.0056 0.0075 0.0008 0.0064 0.0015 0.0054 0.0022
Nucleolus 0.0006 0 0.0001 0.0001 0.0003 0.0001 0.0005 0 0.0001 0 0.0032 0.001 0.0002 0 0.0005 0 0.0003 0.0001
Nucleus 0.0253 0.0105 0.0211 0.032 0.0177 0.0267 0.0263 0.0083 0.0181 0.0144 0.0334 0.0237 0.013 0.0046 0.0146 0.0061 0.0076 0.0083
Peroxisomes 0.0012 0 0.0012 0.0009 0.0086 0 0.0051 0.0001 0.0018 0 0.017 0.0004 0.0046 0 0.0023 0.0058 0.0153 0.0001
Punctate Nuclear 0.0071 0.0005 0.0006 0.0037 0.0053 0.0004 0.01 0.0003 0.0016 0.0002 0.0126 0.0025 0.0043 0.0002 0.0015 0.0006 0.0061 0.0004
Vacuole 0.0034 0.0004 0.0019 0.0031 0.0027 0.0008 0.0039 0.0002 0.0011 0.0003 0.0072 0.0016 0.0017 0.0002 0.0007 0.0004 0.0015 0.0016
Vacuole Periphery 0.002 0.0001 0.0004 0.0014 0.002 0.0003 0.0013 0 0.0005 0.0001 0.004 0.001 0.0008 0 0.0005 0.0003 0.0013 0.0027

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 59.3418 56.7245 48.3279 58.7503 68.5597 44.6285 52.3091 56.2731 60.432 61.5363
Translational Efficiency 2.2263 1.9328 2.2231 1.9289 1.466 2.9166 2.4905 1.9176 1.9843 2.0775

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1663 605 2128 1448 1359 838 142 1024 3022 1443 2270 2472

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1750.64 1928.71 1933.46 2212.42 1622.74 1829.96 2245.10 2278.84 1693.12 1871.36 1952.95 2239.93
Standard Deviation 313.39 355.64 388.93 515.77 302.76 312.44 430.40 555.26 315.14 334.80 398.86 533.49
Intensity Change Log 2 0.139754 0.143302 0.337743 0.173380 0.468348 0.489868 0.156027 0.308811 0.412925

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000164 0.000213 0.000191 0.001014 0.000433 0.000254 0.000835 0.000330 0.000285 0.000237 0.000231 0.000730
Bud Neck 0.000300 0.000593 0.000586 0.003465 0.000397 0.000759 0.008648 0.002233 0.000344 0.000690 0.001090 0.002955
Bud Site 0.002048 0.001677 0.001900 0.017001 0.002310 0.005516 0.006394 0.011166 0.002166 0.003906 0.002181 0.014584
Cell Periphery 0.000104 0.000144 0.000100 0.000282 0.000113 0.000157 0.000643 0.000235 0.000108 0.000152 0.000134 0.000262
Cytoplasm 0.888934* 0.836395* 0.738739* 0.685653* 0.866946* 0.846241* 0.569464* 0.736471* 0.879046* 0.842113* 0.728150* 0.706704*
Cytoplasmic Foci 0.009497 0.008713 0.003868 0.008727 0.007739 0.018378 0.018394 0.012386 0.008707 0.014326 0.004776 0.010243
Eisosomes 0.000001 0.000001 0.000001 0.000008 0.000001 0.000002 0.000015 0.000005 0.000001 0.000002 0.000002 0.000007
Endoplasmic Reticulum 0.000248 0.000389 0.000051 0.000674 0.000537 0.000366 0.001720 0.000189 0.000378 0.000376 0.000156 0.000473
Endosome 0.000248 0.000554 0.000066 0.010563 0.000358 0.001627 0.003819 0.008146 0.000297 0.001177 0.000301 0.009562
Golgi 0.000709 0.001085 0.000133 0.009348 0.001361 0.002896 0.001379 0.010488 0.001002 0.002137 0.000211 0.009820
Lipid Particles 0.000387 0.000512 0.000388 0.001250 0.000447 0.000557 0.003683 0.001497 0.000414 0.000539 0.000594 0.001353
Mitochondria 0.000076 0.000105 0.000132 0.005975 0.000095 0.000124 0.000261 0.006559 0.000085 0.000116 0.000140 0.006217
Mitotic Spindle 0.001585 0.002331 0.002457 0.049513 0.001624 0.005870 0.011480 0.020148 0.001602 0.004386 0.003021 0.037349
None 0.007976 0.011386 0.009727 0.005992 0.007009 0.007389 0.003615 0.007908 0.007541 0.009064 0.009345 0.006785
Nuclear Periphery 0.000086 0.000086 0.000110 0.000578 0.000138 0.000501 0.001545 0.000235 0.000109 0.000327 0.000200 0.000436
Nuclear Periphery Foci 0.003979 0.003185 0.000656 0.005124 0.003518 0.005241 0.010710 0.002088 0.003772 0.004379 0.001285 0.003867
Nucleolus 0.000053 0.000115 0.000076 0.000404 0.000089 0.000132 0.000407 0.000113 0.000069 0.000125 0.000097 0.000283
Nucleus 0.076826 0.124180 0.234425* 0.134188* 0.099019 0.088213 0.217230* 0.150265* 0.086806 0.103293 0.233350* 0.140848*
Peroxisomes 0.000077 0.000031 0.000033 0.000397 0.000025 0.000072 0.000166 0.000290 0.000054 0.000055 0.000041 0.000353
Vacuole 0.005574 0.007768 0.006319 0.043325 0.005856 0.013660 0.138554 0.024136 0.005700 0.011190 0.014591 0.035376
Vacuole Periphery 0.001128 0.000535 0.000041 0.016520 0.001984 0.002046 0.001040 0.005110 0.001513 0.001413 0.000103 0.011793

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.28 -1.63 -2.55 -1.51 -0.93 0.65 -3.05 0.45 -0.65 5.71 -0.15 -0.70 -1.22 -1.56 -0.39
Bud Neck -4.07 -8.87 -11.44 -8.05 -7.57 -5.35 -7.04 -9.62 -6.76 5.51 -6.96 -9.86 -14.92 -10.41 -5.02
Bud Site -0.02 -0.82 -5.34 -5.54 -5.08 -2.75 -6.31 -5.52 -2.97 0.14 -2.77 -1.74 -7.63 -4.95 -6.82
Cell Periphery -2.98 -4.34 -6.10 -2.15 -2.60 -3.48 -7.42 -9.70 -5.59 5.67 -4.68 -7.39 -10.77 -4.98 -2.18
Cytoplasm 9.26 36.48 32.09 17.07 3.48 4.92 18.64 21.56 14.28 -10.54 10.01 38.64 38.12 22.32 0.40
Cytoplasmic Foci -0.20 3.71 0.35 0.53 -4.01 -7.24 -5.65 -7.23 0.39 2.14 -6.32 1.66 -5.36 2.02 -7.01
Eisosomes -2.01 -3.76 -7.88 -5.27 -6.25 -4.08 -7.71 -8.21 -4.06 6.01 -4.69 -7.16 -11.16 -6.16 -5.09
Endoplasmic Reticulum -0.86 4.77 1.25 1.23 -2.88 1.53 -2.64 4.47 4.34 4.04 0.03 4.01 4.33 2.80 0.52
Endosome -1.82 4.81 -3.70 -2.95 -3.97 -2.88 -6.54 -2.41 -0.67 1.45 -3.38 0.19 -4.36 -2.17 -4.38
Golgi -0.80 5.93 -1.72 -1.07 -2.49 -1.05 0.23 -1.13 0.02 -1.46 -1.41 3.19 -1.95 -0.32 -3.56
Lipid Particles -2.10 -2.40 -8.65 -6.36 -3.78 -2.25 -7.71 -6.94 -6.06 3.67 -3.28 -3.89 -10.41 -8.45 -4.43
Mitochondria -0.97 -6.27 -2.14 -2.08 -1.98 -0.76 -4.92 -1.91 -1.88 -1.65 -1.35 -7.73 -2.87 -2.80 -2.67
Mitotic Spindle -0.91 -2.70 -6.14 -5.67 -5.22 -2.67 -2.11 -4.02 -1.36 -0.18 -2.88 -3.16 -7.28 -5.02 -5.61
None -1.75 -1.10 3.97 3.82 9.97 0.29 5.99 2.12 1.44 -5.92 -1.02 -1.65 4.48 4.04 9.74
Nuclear Periphery -1.06 -5.62 -10.70 -11.20 -8.47 -1.32 -6.17 -5.30 0.42 5.27 -1.44 -7.16 -11.47 -0.84 -4.88
Nuclear Periphery Foci 0.26 8.17 2.43 1.95 -3.16 -2.29 -4.72 2.65 3.41 5.38 -1.92 7.64 3.29 3.79 -2.81
Nucleolus -1.27 -8.99 -5.74 -0.34 -4.59 -1.10 -4.77 -1.12 0.55 4.69 -1.72 -3.41 -5.50 -0.51 -4.13
Nucleus -9.58 -39.67 -18.09 -4.38 18.10 1.27 -8.56 -9.27 -9.71 5.11 -6.06 -39.49 -19.45 -10.75 21.16
Peroxisomes 0.98 0.76 -1.12 -3.33 -2.76 -2.76 -7.64 -3.74 -2.13 0.79 -0.07 0.11 -2.68 -3.56 -3.34
Vacuole -3.28 -9.81 -24.61 -20.49 -21.40 -6.20 -10.62 -18.48 -13.19 6.24 -7.06 -12.37 -30.72 -23.36 -19.66
Vacuole Periphery 0.81 1.70 -2.03 -2.97 -3.80 0.17 1.75 0.89 0.97 -1.35 0.38 2.86 -1.22 -1.80 -4.40