Standard name
Description Regulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.14 0.17 0.28 0.21 0.33 0.29 0.27 0.32 0.28 0.33 0.28 0.3 0.22 0.36 0.46 0.2 0.18 0.17 0.07 0.18 0.19 0.21 0.25 0.22
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.09 0.05 0 0 0 0 0 0
Endosome 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.09 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.36 0.45 0.52 0.64 0.52 0.7 0.73 0.71 0.73 0.74 0.75 0.81 0.06 0.06 0.05 0.1 0 0.07 0 0 0 0 0 0
Nucleus 0.45 0.3 0.37 0.41 0.22 0.23 0.16 0.2 0.25 0.24 0.19 0.14 0.4 0.23 0.15 0.42 0.49 0.48 0.55 0.46 0.39 0.47 0.34 0.18
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.15
Vac/Vac Membrane 0.24 0.37 0.13 0.06 0.14 0.08 0.06 0 0.06 0 0 0.05 0.42 0.46 0.46 0.27 0.27 0.31 0.28 0.24 0.3 0.21 0.21 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2
Bud 2 0 0 0 1 5 3 7 0 2 5 1 0 0 1 2 0 0 6 2 2 3 9 23
Bud Neck 0 0 0 0 0 0 0 2 1 0 1 0 0 0 0 0 0 0 2 0 0 0 3 10
Bud Site 2 0 0 1 1 1 0 6 7 6 2 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 46 18 28 29 61 83 97 172 74 89 66 81 72 126 188 33 12 18 31 21 22 40 89 89
Endoplasmic Reticulum 0 1 0 0 0 3 0 0 1 0 0 1 0 0 1 17 6 5 0 0 0 0 2 4
Endosome 23 0 0 1 4 0 0 1 0 1 0 0 9 7 7 13 4 10 5 3 0 3 9 14
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 3
Mitochondria 120 47 52 89 98 202 261 380 194 199 175 218 18 21 20 16 3 8 7 1 5 2 11 14
Nucleus 148 32 37 57 41 65 59 107 67 64 45 39 128 81 61 69 33 52 262 55 46 89 124 75
Nuclear Periphery 0 1 0 1 0 1 1 2 1 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0
Nucleolus 6 1 0 0 1 0 0 0 0 2 0 0 0 1 1 6 0 2 0 0 0 1 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 1 1 5 0 0 0 1 0 0 0 6 2 2 11 3 2 6 24 60
Vac/Vac Membrane 81 39 13 9 26 23 21 24 16 9 8 14 134 163 187 45 18 33 133 29 35 40 75 98
Unique Cell Count 331 105 100 139 187 288 359 536 266 269 232 269 322 354 408 166 67 108 473 122 120 192 362 413
Labelled Cell Count 428 139 130 187 233 384 443 706 361 373 302 357 361 400 467 207 78 131 473 122 120 192 362 413


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 3.9 3.6 3.4 3.7 3.1 2.8 3.1 3.1 2.7 2.9 2.7 4.9 4.6 4.7 5.0 6.0 7.1 4.7 4.5 5.3
Std Deviation (1e-4) 0.6 0.6 0.9 0.7 1.1 1.0 0.9 1.2 1.3 1.2 1.2 1.0 0.9 1.0 1.5 1.3 1.3 1.8 1.1 1.1 1.8
Intensity Change (Log2) – – – -0.06 0.03 -0.21 -0.37 -0.19 -0.22 -0.38 -0.29 -0.4 0.44 0.35 0.39 0.49 0.74 0.99 0.4 0.32 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.7 2.3 3.0