HO
Standard name
Human Ortholog
Description Site-specific endonuclease; required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p, and Ash1p


Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.92 0.99 0.98 0.96 0.95 0.76 0.88 0.68 0.78 0.97 0.87 0.85 0.89 0.66 0.73 0.55 0.64 0.81 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.11 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0.05 0.27 0.13 0.47 0.24 0 0 0 0 0 0.05 0.16 0 0 0
Nucleus 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0.05 0.05 0 0.05 0 0 0 0 0 0 0 0.14 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0.1 0.08 0.07 0.13 0 0.1 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 2 0 0 2 0 0 3 1 0 0 0
Bud 0 0 0 1 0 2 2 5 4 0 0 1 1 0 6 1 0 2 0
Bud Neck 1 5 10 2 14 8 5 5 13 0 0 1 0 0 1 0 0 0 1
Bud Site 0 0 0 0 0 1 0 1 1 0 0 0 0
Cell Periphery 0 0 0 0 1 0 0 0 3 1 2 2 3 0 1 0 0 0 0
Cytoplasm 162 179 248 230 352 157 233 147 231 77 188 159 188 4 129 21 14 45 61
Endoplasmic Reticulum 2 0 0 0 0 0 0 2 1 1 15 9 24 0 0 0 0 0 0
Endosome 2 0 0 1 1 0 1 0 0 0 3 5 6 0 3 0 0 1 0
Golgi 0 0 1 0 0 0 0 0 0 0 0 2 0 0 1 0 0 0 0
Mitochondria 0 1 0 17 19 55 34 102 72 0 7 5 5 0 8 6 0 0 1
Nucleus 9 0 2 1 1 0 2 3 1 0 2 7 3 0 11 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 1 1 1 8 10 12 5 15 0 3 2 3 0 3 1 3 0 2
Vac/Vac Membrane 4 1 3 1 1 2 1 1 3 1 21 14 15 0 4 3 0 0 0
Unique Cell Count 176 181 253 240 372 207 266 217 298 79 215 186 212 7 178 39 23 56 76
Labelled Cell Count 180 187 265 254 397 235 290 272 346 80 241 209 249 7 178 39 23 56 76


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 4.7 5.2 4.2 4.2 3.5 4.1 3.1 3.5 5.2 6.8 5.8 5.3 4.3 4.6
Std Deviation (1e-4) 1.5 1.7 1.8 1.6 1.6 1.4 2.6 1.0 1.2 1.8 3.8 1.4 1.3 1.2 1.8
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2323 -0.14 -0.1989 0.1641 -0.6178 -0.2345 -1.2139 -0.7947 -0.6958 -0.9752 -0.4899 -0.5219 -1.8093 -1.5015 -1.8465 -0.7761 -1.7835 -1.716
Actin 0.0151 0.0032 0.0049 0.0153 0.0014 0.0056 0.0065 0.0002 0.0098 0.0048 0.0044 0.0018 0.0054 0.0001 0.0016 0.0207 0.0009 0.0066
Bud 0.0052 0.0069 0.0034 0.0009 0.0007 0.0017 0.0011 0 0.004 0.0028 0.0027 0.0008 0.0015 0.0001 0.0003 0.0042 0.0001 0.0003
Bud Neck 0.001 0.0007 0.0023 0.0003 0.0001 0.0099 0.0033 0.0001 0.0044 0.0006 0.0008 0.0053 0.0007 0.0001 0.0001 0.0011 0 0.0014
Bud Periphery 0.0101 0.0041 0.0054 0.001 0.0015 0.0039 0.0038 0 0.0074 0.0023 0.007 0.0017 0.0021 0.0003 0.0005 0.008 0.0001 0.0007
Bud Site 0.0067 0.0159 0.0068 0.003 0.0002 0.0006 0.0062 0.0003 0.0057 0.0203 0.0128 0.0011 0.0022 0.001 0.0007 0.0104 0.0002 0.0001
Cell Periphery 0.0004 0.0002 0.0003 0.0001 0.0001 0.0002 0.0006 0.0001 0.0008 0.0002 0.0006 0.0002 0.0004 0.0001 0.0001 0.0006 0 0.0001
Cytoplasm 0.0257 0.0435 0.023 0.0117 0.0197 0.0458 0.0481 0.0801 0.0593 0.046 0.0272 0.0913 0.0518 0.0894 0.0405 0.0345 0.0149 0.0384
Cytoplasmic Foci 0.0283 0.0045 0.0074 0.0134 0.0118 0.0134 0.0147 0.0034 0.0119 0.0218 0.0077 0.0114 0.0287 0.0044 0.0149 0.0431 0.0194 0.0205
Eisosomes 0.0004 0.0002 0.0003 0.0002 0.0004 0.0003 0.0004 0.0001 0.0003 0.0001 0.0003 0.0001 0.0006 0.0001 0.0002 0.0004 0.0004 0.0001
Endoplasmic Reticulum 0.0024 0.0006 0.0006 0.0013 0.0004 0.0006 0.0029 0.0011 0.0025 0.0006 0.0033 0.0015 0.0047 0.002 0.001 0.009 0.0003 0.0008
Endosome 0.0108 0.0011 0.0071 0.0191 0.0021 0.0034 0.0178 0.0025 0.0091 0.0031 0.0231 0.0045 0.0162 0.0016 0.0079 0.057 0.0122 0.0036
Golgi 0.0065 0.0006 0.0023 0.0096 0.0011 0.0018 0.0055 0.0002 0.0034 0.0037 0.0074 0.001 0.0073 0.0001 0.0058 0.0459 0.004 0.0043
Lipid Particles 0.0161 0.0008 0.0024 0.0032 0.0015 0.0017 0.0096 0.0003 0.0084 0.0036 0.0181 0.0085 0.0287 0.0006 0.0039 0.0742 0.0071 0.0046
Mitochondria 0.0076 0.0013 0.0165 0.0081 0.0525 0.0079 0.0031 0.0002 0.0082 0.0022 0.0399 0.0019 0.0202 0.0003 0.0019 0.0231 0.0018 0.001
None 0.7876 0.8097 0.8794 0.8858 0.8748 0.8247 0.8452 0.8143 0.8397 0.8571 0.7966 0.7748 0.7452 0.726 0.904 0.5972 0.9128 0.9114
Nuclear Periphery 0.0087 0.0013 0.0036 0.0018 0.0006 0.0026 0.0029 0.001 0.0021 0.0003 0.0047 0.0013 0.0106 0.0087 0.001 0.0087 0.0002 0.0004
Nucleolus 0.0034 0.0015 0.0023 0.0002 0.0002 0.0018 0.001 0.0009 0.0006 0.0003 0.0188 0.0022 0.0031 0.0011 0.0008 0.0008 0.0001 0.0001
Nucleus 0.0222 0.0911 0.0141 0.0007 0.0006 0.0508 0.0048 0.0913 0.0116 0.0006 0.0056 0.0795 0.0367 0.16 0.001 0.0013 0.0001 0.0012
Peroxisomes 0.0202 0.0032 0.0078 0.0162 0.0287 0.0027 0.0135 0.0001 0.0066 0.0282 0.0051 0.0061 0.0081 0.0001 0.0123 0.0476 0.0242 0.0025
Punctate Nuclear 0.0168 0.0086 0.0071 0.0012 0.0011 0.0193 0.0042 0.0027 0.0007 0.0008 0.0041 0.0027 0.0064 0.0019 0.0007 0.0041 0.0009 0.0013
Vacuole 0.0031 0.0007 0.0025 0.004 0.0005 0.001 0.0042 0.0009 0.0026 0.0007 0.0075 0.002 0.0158 0.0014 0.0008 0.0051 0.0002 0.0004
Vacuole Periphery 0.0019 0.0002 0.0008 0.0028 0.0003 0.0004 0.0006 0.0001 0.0011 0.0001 0.0024 0.0004 0.0036 0.0003 0.0002 0.0029 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.1329 1.6804 5.8189 14.4672 109.6721 6.8885 2.6901 3.0579 10.0761 104.3
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2494 1173 140 985 1728 1455 265 2499 4222 2628 405 3484

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 641.42 683.24 954.85 775.62 669.89 674.79 925.39 820.50 653.07 678.56 935.57 807.81
Standard Deviation 64.69 89.44 111.51 105.55 72.20 101.41 92.78 106.65 69.29 96.34 100.64 108.25
Intensity Change Log 2 0.091123 0.574005 0.274081 0.010514 0.466137 0.292579 0.050507 0.519908 0.283560

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000067 0.000547 0.001134 0.001206 0.000077 0.000415 0.000312 0.000293 0.000071 0.000474 0.000596 0.000551
Bud Neck 0.020180 0.037581 0.001964 0.010814 0.011690 0.050450 0.009387 0.018348 0.016705 0.044706 0.006821 0.016218
Bud Site 0.005658 0.016839 0.005079 0.042715 0.002741 0.020338 0.017814 0.037354 0.004464 0.018776 0.013412 0.038870
Cell Periphery 0.000137 0.000140 0.000437 0.000249 0.000107 0.000428 0.000147 0.000153 0.000125 0.000300 0.000247 0.000180
Cytoplasm 0.545577* 0.380508* 0.343209* 0.381042* 0.520318* 0.297402* 0.320730* 0.492684* 0.535239* 0.334496* 0.328500* 0.461120*
Cytoplasmic Foci 0.146312 0.243039* 0.026756 0.030010 0.191198* 0.246912* 0.012899 0.009075 0.164683 0.245183* 0.017689 0.014994
Eisosomes 0.000143 0.000142 0.000128 0.000064 0.000107 0.000212 0.000081 0.000027 0.000128 0.000180 0.000097 0.000037
Endoplasmic Reticulum 0.000428 0.001209 0.013341 0.003245 0.000659 0.001131 0.005569 0.001093 0.000522 0.001166 0.008256 0.001701
Endosome 0.005976 0.021300 0.009846 0.013077 0.006863 0.041000 0.003993 0.002746 0.006339 0.032207 0.006016 0.005667
Golgi 0.001425 0.005463 0.000246 0.009995 0.001594 0.008251 0.000082 0.000630 0.001494 0.007007 0.000139 0.003278
Lipid Particles 0.006030 0.007423 0.004722 0.002020 0.007346 0.015564 0.003142 0.000575 0.006568 0.011930 0.003688 0.000984
Mitochondria 0.004063 0.017449 0.000312 0.005302 0.005007 0.030357 0.000379 0.004041 0.004450 0.024596 0.000356 0.004398
Mitotic Spindle 0.003997 0.007387 0.000306 0.060232 0.001246 0.013422 0.006973 0.072982 0.002871 0.010728 0.004669 0.069378
None 0.007148 0.003742 0.002179 0.006447 0.004304 0.005544 0.003287 0.007819 0.005984 0.004740 0.002904 0.007431
Nuclear Periphery 0.000355 0.000540 0.001584 0.001457 0.000486 0.000894 0.002449 0.001896 0.000408 0.000736 0.002150 0.001772
Nuclear Periphery Foci 0.000254 0.000350 0.011056 0.001891 0.000236 0.000590 0.002511 0.000594 0.000247 0.000483 0.005465 0.000961
Nucleolus 0.002056 0.002581 0.000725 0.001434 0.001038 0.003798 0.000662 0.001466 0.001639 0.003255 0.000684 0.001457
Nucleus 0.205340* 0.160692* 0.377185* 0.331022* 0.210574* 0.143893* 0.481564* 0.289650* 0.207482* 0.151392* 0.445482* 0.301347*
Peroxisomes 0.002861 0.019655 0.000584 0.000941 0.003610 0.021506 0.000339 0.000641 0.003168 0.020680 0.000424 0.000726
Vacuole 0.041145 0.070742 0.198851 0.095359 0.030139 0.091452 0.127397 0.056198 0.036640 0.082208 0.152097 0.067270
Vacuole Periphery 0.000846 0.002671 0.000354 0.001478 0.000660 0.006439 0.000284 0.001734 0.000770 0.004757 0.000308 0.001661

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.50 -9.07 -5.01 -1.23 2.36 -4.48 -8.05 -9.79 0.92 2.89 -4.25 -11.58 -8.67 -0.12 3.98
Bud Neck -5.69 14.86 1.63 6.29 -8.28 -12.99 2.02 -7.25 9.01 -5.87 -12.97 6.38 -3.43 10.55 -8.32
Bud Site -6.47 -0.58 -8.51 -4.77 -8.41 -9.49 -1.87 -13.34 -2.93 -4.53 -11.01 -1.61 -15.10 -5.39 -7.42
Cell Periphery -0.10 -8.25 -4.82 -3.97 6.43 -2.58 -4.20 -1.20 2.50 3.47 -2.51 -8.01 -2.26 2.30 7.03
Cytoplasm 17.46 12.76 27.77 8.52 0.47 23.79 18.65 19.59 -6.33 -8.33 30.47 23.33 33.23 0.15 -6.81
Cytoplasmic Foci -13.04 28.48 28.02 30.34 -2.30 -7.29 36.22 35.50 38.47 -3.03 -15.37 48.24 48.04 49.85 -3.18
Eisosomes 0.20 -0.26 7.93 5.73 5.12 -9.40 -0.76 11.26 17.07 9.11 -6.71 0.42 16.61 18.29 10.74
Endoplasmic Reticulum -2.32 -10.01 -16.84 -8.32 7.10 -2.12 -12.86 -9.62 -4.04 10.50 -3.36 -15.52 -19.25 -7.59 12.00
Endosome -11.86 -3.44 -2.05 7.61 0.90 -15.74 2.64 6.60 17.82 2.99 -19.32 -0.50 3.52 20.29 3.06
Golgi -5.32 5.82 -2.03 1.28 -3.16 -8.17 7.90 5.10 9.63 -5.46 -9.75 9.37 0.16 8.82 -4.09
Lipid Particles -1.54 1.45 6.31 7.79 5.20 -6.68 5.32 9.22 14.11 7.37 -6.94 5.26 11.87 16.36 9.27
Mitochondria -6.59 6.45 1.53 6.94 -2.04 -9.72 4.59 2.55 11.56 -4.84 -11.97 7.62 3.28 13.62 -4.88
Mitotic Spindle -1.90 3.96 -13.32 -12.77 -13.83 -5.47 -1.88 -22.09 -18.19 -19.51 -5.29 -0.61 -25.53 -22.77 -24.14
None 4.30 6.72 -0.15 -4.87 -7.51 -1.32 3.56 -9.04 -4.59 -15.40 1.78 7.58 -5.74 -7.25 -17.16
Nuclear Periphery -3.33 -11.38 -18.93 -17.31 -5.88 -2.88 -15.19 -16.37 -11.93 -0.84 -4.07 -18.29 -21.93 -17.37 -3.01
Nuclear Periphery Foci -2.08 -10.91 -7.43 -7.33 2.32 -2.45 -2.80 -5.52 -4.42 1.59 -2.84 -5.27 -8.99 -8.24 2.46
Nucleolus -1.08 4.11 -5.24 -4.85 -7.20 -5.18 1.94 -10.49 -5.98 -10.97 -4.37 4.41 -10.95 -7.95 -12.88
Nucleus 6.09 -7.26 -10.75 -13.54 2.07 9.57 -13.40 -15.81 -24.02 7.28 11.38 -15.21 -20.61 -27.50 7.12
Peroxisomes -12.02 8.36 3.07 12.89 -6.50 -12.75 7.55 5.42 15.12 -6.56 -17.69 11.41 6.93 19.99 -8.90
Vacuole -8.89 -12.07 -16.71 -11.19 5.31 -15.22 -13.42 -19.00 -1.78 8.94 -16.87 -17.59 -24.20 -8.41 10.73
Vacuole Periphery -5.60 4.11 1.21 6.14 -1.97 -5.84 5.01 -0.03 5.70 -1.95 -6.98 6.26 0.61 6.94 -2.31