Standard name
Human Ortholog
Description Single stranded DNA-binding protein found at TG1-3 telomere G-tails; key roles in regulation of telomerase, telomere end protection, conventional telomere replication; regulates telomere replication through recruitment of specific sub-complexes, essential function is telomere capping; forms homodimer via N-terminus; disruption of dimerization leads to short telomeres; autophagy and proteasome are involved in Cdc13p degradation; differentially phosphorylated through cell cycle

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0 0.12 0.19 0.17 0.2 0.19 0.19 0.17 0.14 0.12 0.15 0.2 0.27 0.26 0.29 0.28 0.08 0 0.08 0.1 0.13 0.09
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.48 0.68 0.54 0.49 0.27 0.56 0.56 0.62 0.74 0.79 0.81 0 0 0.2 0 0 0.06 0 0 0 0 0 0
Nucleus 0.54 0.55 0.45 0.47 0.44 0.4 0.44 0.4 0.41 0.41 0.34 0.74 0.66 0.5 0.51 0.52 0.52 0.51 0.57 0.47 0.55 0.45 0.38
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0
Vac/Vac Membrane 0.18 0.18 0.15 0.12 0.24 0.12 0.11 0.07 0.08 0.09 0.11 0.16 0.14 0.17 0.26 0.21 0.22 0.18 0.2 0.33 0.26 0.31 0.37
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 1 0 4 7 3 5 11 3 0 0 1 0 0 0 0 1 3 3 6 12
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 1
Bud Site 0 0 0 0 0 3 4 8 11 6 7 0 0 2 0 0 0
Cell Periphery 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 12 0 10 33 47 56 95 114 57 32 45 32 75 120 72 71 76 8 1 7 13 36 39
Endoplasmic Reticulum 0 0 1 1 0 0 0 0 0 0 0 0 2 3 16 11 21 0 0 0 0 1 3
Endosome 0 0 0 0 15 1 0 3 0 0 0 0 3 2 14 6 10 3 1 1 0 0 10
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Mitochondria 44 15 45 86 76 162 276 378 255 184 291 8 11 92 10 6 17 4 3 2 2 6 13
Nucleus 50 12 38 83 125 116 218 240 140 95 124 159 241 224 141 126 141 54 40 48 75 126 173
Nuclear Periphery 1 0 1 1 1 0 4 2 4 2 3 0 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 1 0 2 1 3 1 2 1 2 0 1 1 1 2 0 1 0 0 0 1 3
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 2 3 4 2 3 2 1 1 0 0 0 1 2 1 8 2 2 2 9 17
Vac/Vac Membrane 17 4 13 21 67 35 54 40 26 22 41 35 53 75 73 50 61 18 14 33 35 88 167
Unique Cell Count 92 22 84 175 283 287 494 607 344 233 360 216 367 450 277 243 272 105 72 102 137 283 456
Labelled Cell Count 126 31 109 229 337 382 663 792 502 354 517 234 387 521 328 274 327 105 72 102 137 283 456


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 3.6 3.6 3.8 4.0 3.7 3.3 3.4 3.2 3.2 3.0 4.8 4.8 4.1 6.4 6.6 7.0 4.4 4.5 5.0
Std Deviation (1e-4) 0.7 0.4 0.7 1.3 1.1 1.4 1.0 0.9 1.2 1.2 1.1 1.0 1.3 1.1 1.7 1.7 1.8 0.9 1.0 1.4
Intensity Change (Log2) 0.08 0.14 0.02 -0.11 -0.08 -0.19 -0.17 -0.28 0.42 0.42 0.2 0.83 0.88 0.96 0.3 0.3 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 2.0 1.0 1.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9864 1.4838 1.3742 1.0209 0.8899 1.3072 2.0846 1.3264 1.1605 1.4074 1.5593 1.648 -0.9878 -0.8422 -0.6614 -0.5864 -1.2408 -0.7608
Actin 0.0062 0.0003 0.0072 0.0002 0.0005 0.0013 0.113 0.0003 0.0057 0.0267 0.0036 0.0193 0.0208 0.0002 0.0253 0.0035 0.0048 0.0051
Bud 0.0001 0.0001 0.0001 0 0 0.0001 0.0008 0.0002 0.0002 0.001 0.0002 0.0012 0.0005 0 0.0001 0 0.0002 0.0001
Bud Neck 0.0037 0.0015 0.0072 0.001 0.0013 0.0073 0.0009 0.0006 0.0048 0.0013 0.0003 0.0041 0.002 0.0003 0.002 0.0003 0.0014 0.003
Bud Periphery 0.0002 0.0001 0.0002 0 0 0.0002 0.0012 0.0001 0.0004 0.0023 0.0003 0.0019 0.0006 0 0.0001 0 0.0003 0.0001
Bud Site 0.0008 0.001 0.0007 0.0001 0.0001 0.0003 0.0024 0.0014 0.0049 0.0022 0.0007 0.002 0.004 0.0003 0.0034 0.0001 0.0005 0.0002
Cell Periphery 0.0002 0.0001 0.0001 0 0 0 0.0002 0.0001 0.001 0.0007 0 0.0001 0.0002 0 0.0001 0 0.0001 0
Cytoplasm 0.0452 0.0671 0.0313 0.0286 0.0666 0.0267 0.0868 0.1246 0.052 0.1113 0.009 0.0425 0.0748 0.1094 0.0605 0.0576 0.1221 0.0473
Cytoplasmic Foci 0.0048 0.0023 0.0053 0.0011 0.0019 0.0025 0.0136 0.0019 0.0121 0.0073 0.0236 0.0115 0.0194 0.0014 0.0068 0.0153 0.003 0.0011
Eisosomes 0.0002 0 0.0001 0 0.0001 0.0001 0.0007 0 0.0027 0.0001 0 0.0001 0.0003 0 0.0002 0.0001 0.0001 0
Endoplasmic Reticulum 0.0034 0.0028 0.0021 0.001 0.0033 0.001 0.012 0.0049 0.002 0.0028 0.0008 0.0021 0.0057 0.0048 0.004 0.001 0.005 0.0015
Endosome 0.0052 0.0027 0.0037 0.0012 0.0031 0.0021 0.0455 0.003 0.0125 0.0164 0.0505 0.0233 0.0142 0.0016 0.0069 0.0009 0.0046 0.0032
Golgi 0.0009 0.0001 0.0008 0 0.0001 0.0001 0.016 0.0001 0.0047 0.0056 0.0464 0.0194 0.0069 0 0.0037 0.0008 0.0003 0.0009
Lipid Particles 0.0056 0.0004 0.0022 0.0001 0.0004 0.0006 0.0266 0.0002 0.0029 0.0037 0.0294 0.0066 0.0183 0.0002 0.0051 0.0051 0.0004 0.0011
Mitochondria 0.0008 0.0006 0.0018 0.0005 0.0007 0.0005 0.0131 0.0008 0.0012 0.0203 0.0187 0.0071 0.0044 0.0005 0.0008 0.0006 0.0015 0.0008
None 0.1131 0.101 0.0788 0.0564 0.1485 0.0665 0.0383 0.0579 0.0848 0.0259 0.0997 0.0275 0.1378 0.1945 0.1809 0.2248 0.2001 0.0883
Nuclear Periphery 0.0243 0.0279 0.048 0.0207 0.033 0.0109 0.0497 0.0207 0.0218 0.0278 0.0134 0.0111 0.0233 0.0324 0.0491 0.012 0.035 0.0125
Nucleolus 0.0105 0.0152 0.011 0.008 0.0151 0.0188 0.0025 0.0061 0.0066 0.0052 0.0017 0.0051 0.0046 0.0048 0.0063 0.0022 0.0032 0.009
Nucleus 0.6846 0.7296 0.6694 0.7885 0.624 0.7893 0.5217 0.7479 0.7442 0.655 0.5709 0.7818 0.6256 0.6345 0.5418 0.629 0.5759 0.7811
Peroxisomes 0.0013 0.0002 0.0022 0.0001 0.0001 0.0002 0.0078 0.0001 0.001 0.0028 0.0114 0.0122 0.0137 0 0.0015 0.033 0.0005 0.0002
Punctate Nuclear 0.0861 0.0449 0.1266 0.0916 0.0994 0.0703 0.0307 0.0253 0.0328 0.0487 0.1156 0.0187 0.0189 0.0132 0.0993 0.0133 0.0387 0.0433
Vacuole 0.002 0.0018 0.0011 0.0007 0.0013 0.0008 0.0112 0.003 0.0012 0.0274 0.0015 0.0018 0.0028 0.0014 0.0014 0.0003 0.0015 0.0009
Vacuole Periphery 0.0005 0.0004 0.0005 0.0002 0.0005 0.0002 0.0051 0.0005 0.0006 0.0052 0.0021 0.0007 0.0011 0.0004 0.0008 0.0001 0.0008 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.3263 5.3318 6.3302 7.7697 10.0529 6.7559 8.0864 5.8216 7.7237 10.9926
Translational Efficiency 0.4615 0.3826 0.5155 0.3587 0.3772 0.9535 0.3588 0.4575 0.3984 0.4347

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
829 1299 318 277 699 1850 179 109 1528 3149 497 386

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 611.72 737.72 912.86 870.04 670.38 711.35 943.93 907.97 638.55 722.23 924.05 880.75
Standard Deviation 80.26 96.96 93.11 116.65 75.64 90.11 99.77 92.88 83.46 93.90 96.72 111.77
Intensity Change Log 2 0.270202 0.577522 0.508210 0.085580 0.493701 0.437666 0.176618 0.534302 0.471755

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000059 0.000196 0.000749 0.000648 0.000063 0.000205 0.001130 0.000536 0.000061 0.000201 0.000886 0.000616
Bud Neck 0.002252 0.008831 0.004276 0.008807 0.002922 0.013450 0.003466 0.007822 0.002559 0.011545 0.003984 0.008529
Bud Site 0.003803 0.002737 0.001772 0.004398 0.000408 0.006139 0.001419 0.004403 0.002250 0.004736 0.001645 0.004399
Cell Periphery 0.000203 0.000113 0.000100 0.000193 0.000112 0.000326 0.000142 0.000102 0.000162 0.000238 0.000115 0.000168
Cytoplasm 0.418015* 0.160360* 0.014744 0.088757 0.186062* 0.202473* 0.017951 0.047246 0.311906* 0.185101* 0.015899 0.077035
Cytoplasmic Foci 0.071142 0.029103 0.000763 0.004150 0.025088 0.039523 0.000844 0.001441 0.050074 0.035225 0.000792 0.003385
Eisosomes 0.000070 0.000044 0.000057 0.000054 0.000032 0.000055 0.000072 0.000041 0.000053 0.000050 0.000062 0.000050
Endoplasmic Reticulum 0.000518 0.003005 0.012213 0.009261 0.001147 0.002038 0.018095 0.010006 0.000806 0.002437 0.014332 0.009472
Endosome 0.001876 0.003885 0.000886 0.005949 0.000857 0.003059 0.001337 0.003130 0.001410 0.003400 0.001048 0.005153
Golgi 0.000799 0.000455 0.000065 0.006069 0.000131 0.000986 0.000093 0.000073 0.000493 0.000767 0.000075 0.004376
Lipid Particles 0.005975 0.003681 0.001150 0.001665 0.003925 0.005680 0.001780 0.000908 0.005037 0.004855 0.001377 0.001451
Mitochondria 0.002854 0.004736 0.001173 0.003050 0.002223 0.007009 0.000813 0.000717 0.002565 0.006072 0.001043 0.002391
Mitotic Spindle 0.001410 0.003320 0.000605 0.026857 0.002878 0.012787 0.000565 0.001006 0.002082 0.008882 0.000590 0.019557
None 0.016809 0.007821 0.001892 0.009289 0.006742 0.005035 0.003605 0.004328 0.012204 0.006184 0.002509 0.007888
Nuclear Periphery 0.000558 0.002378 0.001578 0.005095 0.001790 0.002414 0.003167 0.001887 0.001122 0.002399 0.002150 0.004189
Nuclear Periphery Foci 0.000761 0.002246 0.000864 0.005164 0.001070 0.001182 0.015275 0.001105 0.000903 0.001621 0.006054 0.004018
Nucleolus 0.002590 0.006557 0.002173 0.002398 0.004775 0.005751 0.004349 0.001098 0.003590 0.006083 0.002957 0.002031
Nucleus 0.457857* 0.742108* 0.941003* 0.771182* 0.753755* 0.673687* 0.904091* 0.865492* 0.593219* 0.701912* 0.927709* 0.797813*
Peroxisomes 0.001416 0.003569 0.000354 0.000423 0.001394 0.004301 0.000505 0.000380 0.001406 0.003999 0.000408 0.000411
Vacuole 0.010770 0.014278 0.013387 0.042000 0.004494 0.013102 0.021120 0.048000 0.007899 0.013587 0.016172 0.043694
Vacuole Periphery 0.000262 0.000573 0.000197 0.004592 0.000131 0.000800 0.000181 0.000279 0.000202 0.000706 0.000191 0.003374

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.10 -7.23 -10.74 -8.95 -0.30 -3.55 -8.34 -6.10 -5.03 2.66 -6.41 -10.63 -12.27 -10.15 1.20
Bud Neck -6.17 -4.60 -7.42 -2.22 -4.11 -7.24 -2.20 -3.15 2.42 -1.76 -9.63 -4.87 -7.86 -0.04 -4.57
Bud Site 0.46 0.51 -1.94 -3.19 -3.09 -5.62 -5.53 -3.13 1.10 -1.40 -2.38 -0.46 -3.35 -1.65 -3.45
Cell Periphery 2.51 2.09 0.13 -4.16 -3.13 -1.15 -2.34 -0.14 1.13 2.29 -0.70 0.86 -0.54 0.58 -1.82
Cytoplasm 24.24 42.20 27.28 7.96 -6.65 -1.81 18.83 11.54 15.02 -2.65 16.29 40.95 25.44 15.03 -7.11
Cytoplasmic Foci 9.09 19.09 17.67 11.46 -2.79 -4.03 9.84 9.72 17.90 -0.09 4.77 20.86 19.16 19.66 -2.71
Eisosomes 3.76 -0.31 -2.58 -5.35 -1.66 -1.56 -5.03 -3.13 -0.74 2.71 0.32 -3.45 -4.33 -3.71 -0.13
Endoplasmic Reticulum -12.77 -11.97 -13.46 -10.73 0.15 -3.63 -8.48 -8.33 -7.84 1.81 -11.36 -14.28 -15.68 -13.71 1.40
Endosome -2.42 2.72 -3.20 -0.42 -4.28 -6.09 -3.32 -1.78 0.76 -0.21 -4.85 -0.35 -3.96 -0.58 -3.80
Golgi 0.84 2.28 -0.70 -0.85 -1.08 -2.79 -0.22 0.92 2.95 1.31 -1.17 2.27 -0.78 -0.56 -1.06
Lipid Particles 1.28 3.53 2.53 2.72 -2.19 -1.88 2.08 4.35 7.62 2.70 -0.18 4.30 3.72 6.14 -0.70
Mitochondria -1.19 1.14 0.52 2.16 -1.00 -4.45 2.48 2.09 6.70 -1.32 -3.56 1.88 0.94 5.28 -1.19
Mitotic Spindle -1.86 0.74 -3.86 -3.51 -3.95 -4.02 0.76 -1.58 -0.40 -1.75 -4.76 1.14 -4.12 -2.90 -4.31
None 4.06 6.98 3.68 0.32 -3.42 2.00 3.84 0.98 -0.04 -1.25 4.81 8.03 3.33 -0.34 -3.56
Nuclear Periphery -9.95 -8.39 -7.73 -4.67 -5.45 -2.13 -2.25 -3.12 -2.60 -1.48 -7.86 -4.83 -7.75 -5.43 -4.97
Nuclear Periphery Foci -3.05 -1.19 -2.51 -1.62 -2.34 0.03 -2.67 -1.19 -1.23 2.41 -2.77 -2.73 -2.60 -2.07 0.08
Nucleolus -4.96 0.28 -0.85 3.83 -1.09 -0.34 -0.30 2.57 3.65 1.73 -3.03 0.12 1.68 5.13 1.06
Nucleus -21.66 -38.03 -12.60 0.86 10.79 6.64 -6.03 -1.61 -4.65 2.26 -10.85 -28.40 -8.71 -2.74 9.83
Peroxisomes -3.72 3.36 2.81 5.42 -0.55 -3.78 1.80 3.53 7.03 2.33 -5.41 3.70 4.18 8.53 0.84
Vacuole -2.71 -3.43 -8.24 -7.73 -6.67 -5.72 -4.93 -4.95 -4.48 -2.95 -5.60 -6.07 -9.71 -9.00 -6.98
Vacuole Periphery -2.81 0.39 -1.22 -0.97 -1.25 -4.67 -3.01 -1.53 3.84 0.06 -5.10 -0.39 -1.28 -0.70 -1.25
Standard name
Human Ortholog
Description Single stranded DNA-binding protein found at TG1-3 telomere G-tails; key roles in regulation of telomerase, telomere end protection, conventional telomere replication; regulates telomere replication through recruitment of specific sub-complexes, essential function is telomere capping; forms homodimer via N-terminus; disruption of dimerization leads to short telomeres; autophagy and proteasome are involved in Cdc13p degradation; differentially phosphorylated through cell cycle
Localization
Cell Percentages nucleus (39%), cytoplasm (9%), mixed (41%)
Cell Cycle Regulation Yes
cytoplasm - MA (2.3e-10)
Subcompartmental Group N/A

Cdc13

Cdc13


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc13-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available