Standard name
Description Single stranded DNA-binding protein found at TG1-3 telomere G-tails; key roles in regulation of telomerase, telomere end protection, conventional telomere replication; regulates telomere replication through recruitment of specific sub-complexes, essential function is telomere capping; forms homodimer via N-terminus; disruption of dimerization leads to short telomeres; autophagy and proteasome are involved in Cdc13p degradation; differentially phosphorylated through cell cycle
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0 0.12 0.19 0.17 0.2 0.19 0.19 0.17 – 0.14 0.12 0.15 0.2 0.27 0.26 0.29 0.28 0.08 0 0.08 0.1 0.13 0.09
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.05 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.48 0.68 0.54 0.49 0.27 0.56 0.56 0.62 0.74 – 0.79 0.81 0 0 0.2 0 0 0.06 0 0 0 0 0 0
Nucleus 0.54 0.55 0.45 0.47 0.44 0.4 0.44 0.4 0.41 – 0.41 0.34 0.74 0.66 0.5 0.51 0.52 0.52 0.51 0.57 0.47 0.55 0.45 0.38
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0
Vac/Vac Membrane 0.18 0.18 0.15 0.12 0.24 0.12 0.11 0.07 0.08 – 0.09 0.11 0.16 0.14 0.17 0.26 0.21 0.22 0.18 0.2 0.33 0.26 0.31 0.37
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 1 0 4 7 3 5 – 11 3 0 0 1 0 0 0 0 1 3 3 6 12
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 1 1 1
Bud Site 0 0 0 0 0 3 4 8 11 – 6 7 0 0 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 12 0 10 33 47 56 95 114 57 – 32 45 32 75 120 72 71 76 8 1 7 13 36 39
Endoplasmic Reticulum 0 0 1 1 0 0 0 0 0 – 0 0 0 2 3 16 11 21 0 0 0 0 1 3
Endosome 0 0 0 0 15 1 0 3 0 – 0 0 0 3 2 14 6 10 3 1 1 0 0 10
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Mitochondria 44 15 45 86 76 162 276 378 255 – 184 291 8 11 92 10 6 17 4 3 2 2 6 13
Nucleus 50 12 38 83 125 116 218 240 140 – 95 124 159 241 224 141 126 141 54 40 48 75 126 173
Nuclear Periphery 1 0 1 1 1 0 4 2 4 – 2 3 0 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 1 0 2 1 3 1 2 – 1 2 0 1 1 1 2 0 1 0 0 0 1 3
Peroxisomes 0 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 2 3 4 2 3 2 – 1 1 0 0 0 1 2 1 8 2 2 2 9 17
Vac/Vac Membrane 17 4 13 21 67 35 54 40 26 – 22 41 35 53 75 73 50 61 18 14 33 35 88 167
Unique Cell Count 92 22 84 175 283 287 494 607 344 233 360 216 367 450 277 243 272 105 72 102 137 283 456
Labelled Cell Count 126 31 109 229 337 382 663 792 502 354 517 234 387 521 328 274 327 105 72 102 137 283 456


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 3.6 3.6 3.8 4.0 3.7 3.3 3.4 3.2 – 3.2 3.0 4.8 4.8 4.1 6.4 6.6 7.0 4.4 4.5 5.0
Std Deviation (1e-4) 0.7 0.4 0.7 1.3 1.1 1.4 1.0 0.9 1.2 – 1.2 1.1 1.0 1.3 1.1 1.7 1.7 1.8 0.9 1.0 1.4
Intensity Change (Log2) – – – 0.08 0.14 0.02 -0.11 -0.08 -0.19 – -0.17 -0.28 0.42 0.42 0.2 0.83 0.88 0.96 0.3 0.3 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.0 1.0 1.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9864 1.4838 1.3742 1.0209 0.8899 1.3072 2.0846 1.3264 1.1605 1.4074 1.5593 1.648 -0.9878 -0.8422 -0.6614 -0.5864 -1.2408 -0.7608
Actin 0.0062 0.0003 0.0072 0.0002 0.0005 0.0013 0.113 0.0003 0.0057 0.0267 0.0036 0.0193 0.0208 0.0002 0.0253 0.0035 0.0048 0.0051
Bud 0.0001 0.0001 0.0001 0 0 0.0001 0.0008 0.0002 0.0002 0.001 0.0002 0.0012 0.0005 0 0.0001 0 0.0002 0.0001
Bud Neck 0.0037 0.0015 0.0072 0.001 0.0013 0.0073 0.0009 0.0006 0.0048 0.0013 0.0003 0.0041 0.002 0.0003 0.002 0.0003 0.0014 0.003
Bud Periphery 0.0002 0.0001 0.0002 0 0 0.0002 0.0012 0.0001 0.0004 0.0023 0.0003 0.0019 0.0006 0 0.0001 0 0.0003 0.0001
Bud Site 0.0008 0.001 0.0007 0.0001 0.0001 0.0003 0.0024 0.0014 0.0049 0.0022 0.0007 0.002 0.004 0.0003 0.0034 0.0001 0.0005 0.0002
Cell Periphery 0.0002 0.0001 0.0001 0 0 0 0.0002 0.0001 0.001 0.0007 0 0.0001 0.0002 0 0.0001 0 0.0001 0
Cytoplasm 0.0452 0.0671 0.0313 0.0286 0.0666 0.0267 0.0868 0.1246 0.052 0.1113 0.009 0.0425 0.0748 0.1094 0.0605 0.0576 0.1221 0.0473
Cytoplasmic Foci 0.0048 0.0023 0.0053 0.0011 0.0019 0.0025 0.0136 0.0019 0.0121 0.0073 0.0236 0.0115 0.0194 0.0014 0.0068 0.0153 0.003 0.0011
Eisosomes 0.0002 0 0.0001 0 0.0001 0.0001 0.0007 0 0.0027 0.0001 0 0.0001 0.0003 0 0.0002 0.0001 0.0001 0
Endoplasmic Reticulum 0.0034 0.0028 0.0021 0.001 0.0033 0.001 0.012 0.0049 0.002 0.0028 0.0008 0.0021 0.0057 0.0048 0.004 0.001 0.005 0.0015
Endosome 0.0052 0.0027 0.0037 0.0012 0.0031 0.0021 0.0455 0.003 0.0125 0.0164 0.0505 0.0233 0.0142 0.0016 0.0069 0.0009 0.0046 0.0032
Golgi 0.0009 0.0001 0.0008 0 0.0001 0.0001 0.016 0.0001 0.0047 0.0056 0.0464 0.0194 0.0069 0 0.0037 0.0008 0.0003 0.0009
Lipid Particles 0.0056 0.0004 0.0022 0.0001 0.0004 0.0006 0.0266 0.0002 0.0029 0.0037 0.0294 0.0066 0.0183 0.0002 0.0051 0.0051 0.0004 0.0011
Mitochondria 0.0008 0.0006 0.0018 0.0005 0.0007 0.0005 0.0131 0.0008 0.0012 0.0203 0.0187 0.0071 0.0044 0.0005 0.0008 0.0006 0.0015 0.0008
None 0.1131 0.101 0.0788 0.0564 0.1485 0.0665 0.0383 0.0579 0.0848 0.0259 0.0997 0.0275 0.1378 0.1945 0.1809 0.2248 0.2001 0.0883
Nuclear Periphery 0.0243 0.0279 0.048 0.0207 0.033 0.0109 0.0497 0.0207 0.0218 0.0278 0.0134 0.0111 0.0233 0.0324 0.0491 0.012 0.035 0.0125
Nucleolus 0.0105 0.0152 0.011 0.008 0.0151 0.0188 0.0025 0.0061 0.0066 0.0052 0.0017 0.0051 0.0046 0.0048 0.0063 0.0022 0.0032 0.009
Nucleus 0.6846 0.7296 0.6694 0.7885 0.624 0.7893 0.5217 0.7479 0.7442 0.655 0.5709 0.7818 0.6256 0.6345 0.5418 0.629 0.5759 0.7811
Peroxisomes 0.0013 0.0002 0.0022 0.0001 0.0001 0.0002 0.0078 0.0001 0.001 0.0028 0.0114 0.0122 0.0137 0 0.0015 0.033 0.0005 0.0002
Punctate Nuclear 0.0861 0.0449 0.1266 0.0916 0.0994 0.0703 0.0307 0.0253 0.0328 0.0487 0.1156 0.0187 0.0189 0.0132 0.0993 0.0133 0.0387 0.0433
Vacuole 0.002 0.0018 0.0011 0.0007 0.0013 0.0008 0.0112 0.003 0.0012 0.0274 0.0015 0.0018 0.0028 0.0014 0.0014 0.0003 0.0015 0.0009
Vacuole Periphery 0.0005 0.0004 0.0005 0.0002 0.0005 0.0002 0.0051 0.0005 0.0006 0.0052 0.0021 0.0007 0.0011 0.0004 0.0008 0.0001 0.0008 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.3263 5.3318 6.3302 7.7697 10.0529 6.7559 8.0864 5.8216 7.7237 10.9926
Translational Efficiency 0.4615 0.3826 0.5155 0.3587 0.3772 0.9535 0.3588 0.4575 0.3984 0.4347

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘