Standard name | |
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Description | Single stranded DNA-binding protein found at TG1-3 telomere G-tails; key roles in regulation of telomerase, telomere end protection, conventional telomere replication; regulates telomere replication through recruitment of specific sub-complexes, essential function is telomere capping; forms homodimer via N-terminus; disruption of dimerization leads to short telomeres; autophagy and proteasome are involved in Cdc13p degradation; differentially phosphorylated through cell cycle |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 1 | 0 | 4 | 7 | 3 | 5 | – | 11 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 3 | 6 | 12 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 8 | 11 | – | 6 | 7 | 0 | 0 | 2 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 12 | 0 | 10 | 33 | 47 | 56 | 95 | 114 | 57 | – | 32 | 45 | 32 | 75 | 120 | 72 | 71 | 76 | 8 | 1 | 7 | 13 | 36 | 39 |
Endoplasmic Reticulum | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 3 | 16 | 11 | 21 | 0 | 0 | 0 | 0 | 1 | 3 |
Endosome | 0 | 0 | 0 | 0 | 15 | 1 | 0 | 3 | 0 | – | 0 | 0 | 0 | 3 | 2 | 14 | 6 | 10 | 3 | 1 | 1 | 0 | 0 | 10 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Mitochondria | 44 | 15 | 45 | 86 | 76 | 162 | 276 | 378 | 255 | – | 184 | 291 | 8 | 11 | 92 | 10 | 6 | 17 | 4 | 3 | 2 | 2 | 6 | 13 |
Nucleus | 50 | 12 | 38 | 83 | 125 | 116 | 218 | 240 | 140 | – | 95 | 124 | 159 | 241 | 224 | 141 | 126 | 141 | 54 | 40 | 48 | 75 | 126 | 173 |
Nuclear Periphery | 1 | 0 | 1 | 1 | 1 | 0 | 4 | 2 | 4 | – | 2 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 1 | 0 | 2 | 1 | 3 | 1 | 2 | – | 1 | 2 | 0 | 1 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 3 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 2 | 3 | 4 | 2 | 3 | 2 | – | 1 | 1 | 0 | 0 | 0 | 1 | 2 | 1 | 8 | 2 | 2 | 2 | 9 | 17 |
Vac/Vac Membrane | 17 | 4 | 13 | 21 | 67 | 35 | 54 | 40 | 26 | – | 22 | 41 | 35 | 53 | 75 | 73 | 50 | 61 | 18 | 14 | 33 | 35 | 88 | 167 |
Unique Cell Count | 92 | 22 | 84 | 175 | 283 | 287 | 494 | 607 | 344 | 233 | 360 | 216 | 367 | 450 | 277 | 243 | 272 | 105 | 72 | 102 | 137 | 283 | 456 | |
Labelled Cell Count | 126 | 31 | 109 | 229 | 337 | 382 | 663 | 792 | 502 | 354 | 517 | 234 | 387 | 521 | 328 | 274 | 327 | 105 | 72 | 102 | 137 | 283 | 456 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.0 | 3.6 | 3.6 | 3.8 | 4.0 | 3.7 | 3.3 | 3.4 | 3.2 | – | 3.2 | 3.0 | 4.8 | 4.8 | 4.1 | 6.4 | 6.6 | 7.0 | 4.4 | 4.5 | 5.0 |
Std Deviation (1e-4) | 0.7 | 0.4 | 0.7 | 1.3 | 1.1 | 1.4 | 1.0 | 0.9 | 1.2 | – | 1.2 | 1.1 | 1.0 | 1.3 | 1.1 | 1.7 | 1.7 | 1.8 | 0.9 | 1.0 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.08 | 0.14 | 0.02 | -0.11 | -0.08 | -0.19 | – | -0.17 | -0.28 | 0.42 | 0.42 | 0.2 | 0.83 | 0.88 | 0.96 | 0.3 | 0.3 | 0.48 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 2.0 | 1.0 | 1.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.9864 | 1.4838 | 1.3742 | 1.0209 | 0.8899 | 1.3072 | 2.0846 | 1.3264 | 1.1605 | 1.4074 | 1.5593 | 1.648 | -0.9878 | -0.8422 | -0.6614 | -0.5864 | -1.2408 | -0.7608 |
Actin | 0.0062 | 0.0003 | 0.0072 | 0.0002 | 0.0005 | 0.0013 | 0.113 | 0.0003 | 0.0057 | 0.0267 | 0.0036 | 0.0193 | 0.0208 | 0.0002 | 0.0253 | 0.0035 | 0.0048 | 0.0051 |
Bud | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0008 | 0.0002 | 0.0002 | 0.001 | 0.0002 | 0.0012 | 0.0005 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 |
Bud Neck | 0.0037 | 0.0015 | 0.0072 | 0.001 | 0.0013 | 0.0073 | 0.0009 | 0.0006 | 0.0048 | 0.0013 | 0.0003 | 0.0041 | 0.002 | 0.0003 | 0.002 | 0.0003 | 0.0014 | 0.003 |
Bud Periphery | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0.0002 | 0.0012 | 0.0001 | 0.0004 | 0.0023 | 0.0003 | 0.0019 | 0.0006 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 |
Bud Site | 0.0008 | 0.001 | 0.0007 | 0.0001 | 0.0001 | 0.0003 | 0.0024 | 0.0014 | 0.0049 | 0.0022 | 0.0007 | 0.002 | 0.004 | 0.0003 | 0.0034 | 0.0001 | 0.0005 | 0.0002 |
Cell Periphery | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.001 | 0.0007 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0452 | 0.0671 | 0.0313 | 0.0286 | 0.0666 | 0.0267 | 0.0868 | 0.1246 | 0.052 | 0.1113 | 0.009 | 0.0425 | 0.0748 | 0.1094 | 0.0605 | 0.0576 | 0.1221 | 0.0473 |
Cytoplasmic Foci | 0.0048 | 0.0023 | 0.0053 | 0.0011 | 0.0019 | 0.0025 | 0.0136 | 0.0019 | 0.0121 | 0.0073 | 0.0236 | 0.0115 | 0.0194 | 0.0014 | 0.0068 | 0.0153 | 0.003 | 0.0011 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0007 | 0 | 0.0027 | 0.0001 | 0 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0034 | 0.0028 | 0.0021 | 0.001 | 0.0033 | 0.001 | 0.012 | 0.0049 | 0.002 | 0.0028 | 0.0008 | 0.0021 | 0.0057 | 0.0048 | 0.004 | 0.001 | 0.005 | 0.0015 |
Endosome | 0.0052 | 0.0027 | 0.0037 | 0.0012 | 0.0031 | 0.0021 | 0.0455 | 0.003 | 0.0125 | 0.0164 | 0.0505 | 0.0233 | 0.0142 | 0.0016 | 0.0069 | 0.0009 | 0.0046 | 0.0032 |
Golgi | 0.0009 | 0.0001 | 0.0008 | 0 | 0.0001 | 0.0001 | 0.016 | 0.0001 | 0.0047 | 0.0056 | 0.0464 | 0.0194 | 0.0069 | 0 | 0.0037 | 0.0008 | 0.0003 | 0.0009 |
Lipid Particles | 0.0056 | 0.0004 | 0.0022 | 0.0001 | 0.0004 | 0.0006 | 0.0266 | 0.0002 | 0.0029 | 0.0037 | 0.0294 | 0.0066 | 0.0183 | 0.0002 | 0.0051 | 0.0051 | 0.0004 | 0.0011 |
Mitochondria | 0.0008 | 0.0006 | 0.0018 | 0.0005 | 0.0007 | 0.0005 | 0.0131 | 0.0008 | 0.0012 | 0.0203 | 0.0187 | 0.0071 | 0.0044 | 0.0005 | 0.0008 | 0.0006 | 0.0015 | 0.0008 |
None | 0.1131 | 0.101 | 0.0788 | 0.0564 | 0.1485 | 0.0665 | 0.0383 | 0.0579 | 0.0848 | 0.0259 | 0.0997 | 0.0275 | 0.1378 | 0.1945 | 0.1809 | 0.2248 | 0.2001 | 0.0883 |
Nuclear Periphery | 0.0243 | 0.0279 | 0.048 | 0.0207 | 0.033 | 0.0109 | 0.0497 | 0.0207 | 0.0218 | 0.0278 | 0.0134 | 0.0111 | 0.0233 | 0.0324 | 0.0491 | 0.012 | 0.035 | 0.0125 |
Nucleolus | 0.0105 | 0.0152 | 0.011 | 0.008 | 0.0151 | 0.0188 | 0.0025 | 0.0061 | 0.0066 | 0.0052 | 0.0017 | 0.0051 | 0.0046 | 0.0048 | 0.0063 | 0.0022 | 0.0032 | 0.009 |
Nucleus | 0.6846 | 0.7296 | 0.6694 | 0.7885 | 0.624 | 0.7893 | 0.5217 | 0.7479 | 0.7442 | 0.655 | 0.5709 | 0.7818 | 0.6256 | 0.6345 | 0.5418 | 0.629 | 0.5759 | 0.7811 |
Peroxisomes | 0.0013 | 0.0002 | 0.0022 | 0.0001 | 0.0001 | 0.0002 | 0.0078 | 0.0001 | 0.001 | 0.0028 | 0.0114 | 0.0122 | 0.0137 | 0 | 0.0015 | 0.033 | 0.0005 | 0.0002 |
Punctate Nuclear | 0.0861 | 0.0449 | 0.1266 | 0.0916 | 0.0994 | 0.0703 | 0.0307 | 0.0253 | 0.0328 | 0.0487 | 0.1156 | 0.0187 | 0.0189 | 0.0132 | 0.0993 | 0.0133 | 0.0387 | 0.0433 |
Vacuole | 0.002 | 0.0018 | 0.0011 | 0.0007 | 0.0013 | 0.0008 | 0.0112 | 0.003 | 0.0012 | 0.0274 | 0.0015 | 0.0018 | 0.0028 | 0.0014 | 0.0014 | 0.0003 | 0.0015 | 0.0009 |
Vacuole Periphery | 0.0005 | 0.0004 | 0.0005 | 0.0002 | 0.0005 | 0.0002 | 0.0051 | 0.0005 | 0.0006 | 0.0052 | 0.0021 | 0.0007 | 0.0011 | 0.0004 | 0.0008 | 0.0001 | 0.0008 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.3263 | 5.3318 | 6.3302 | 7.7697 | 10.0529 | 6.7559 | 8.0864 | 5.8216 | 7.7237 | 10.9926 |
Translational Efficiency | 0.4615 | 0.3826 | 0.5155 | 0.3587 | 0.3772 | 0.9535 | 0.3588 | 0.4575 | 0.3984 | 0.4347 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |