Standard name
Description Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.07 0.12 0.11 0.09 0.07 0.09 0.07 0.06 0.07 0 0.12 0.14 0.17 0 0.05 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.3 0 0 0.08 0.09 0.4 0.54 0.45 0.58 0.51 0.72 0.42 0.06 0 0 0 0.05 0.06 0 0 0 0 0 0
Nucleus 0.93 0.93 0.9 0.85 0.84 0.75 0.75 0.77 0.72 0.79 0.71 0.87 0.84 0.85 0.82 0.93 0.84 0.85 0.88 0.88 0.81 0.81 0.69 0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.08 0 0.06 0.12 0.09 0.16 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud 1 0 0 1 0 0 4 2 4 1 7 2 0 0 0 0 0 0 3 1 1 1 7 8
Bud Neck 0 0 1 0 0 0 0 2 0 2 1 0 2 0 0 0 0 0 0 0 0 0 2 3
Bud Site 0 0 0 0 4 7 11 11 9 10 21 14 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 3 4 16 20 25 24 23 24 20 19 21 10 39 79 90 7 9 9 1 1 1 2 5 3
Endoplasmic Reticulum 0 0 1 1 1 0 0 0 0 0 0 0 3 0 1 3 3 4 1 0 0 1 1 2
Endosome 0 0 1 1 0 3 0 0 0 0 0 0 1 2 2 2 2 0 3 0 1 0 2 6
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1 0 0 0 1
Mitochondria 85 0 7 14 20 104 171 118 171 161 205 113 19 0 2 5 9 9 1 1 2 5 9 12
Nucleus 268 115 209 142 189 194 236 202 212 246 204 234 281 486 442 212 147 131 252 122 215 231 336 339
Nuclear Periphery 1 0 0 0 0 2 1 0 0 0 2 2 0 0 0 1 2 1 0 0 0 0 2 3
Nucleolus 1 0 0 0 0 0 1 1 3 2 3 4 1 1 0 0 0 4 3 1 0 4 23 23
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 4 0 5 2 2 3 1 2 0 2 1 3 5 2 0 1 2 0 2 5 14 9
Vac/Vac Membrane 2 6 2 1 5 5 5 3 3 2 4 3 3 9 6 10 18 13 12 8 31 24 78 63
Unique Cell Count 287 124 232 168 225 259 314 264 293 313 286 268 334 571 540 229 176 155 288 139 266 285 491 485
Labelled Cell Count 361 125 241 180 250 341 454 366 423 446 468 384 350 580 550 243 191 173 288 139 266 285 491 485


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 6.2 5.4 5.3 5.3 4.1 4.0 4.4 3.7 4.0 3.6 4.1 5.6 6.0 5.9 8.0 7.6 7.2 5.7 5.5 6.3
Std Deviation (1e-4) 0.7 1.1 1.0 1.6 2.1 1.2 1.7 1.3 1.1 1.3 1.2 1.3 1.8 1.2 1.3 1.4 1.5 1.4 1.4 1.0 1.9
Intensity Change (Log2) -0.03 -0.01 -0.38 -0.44 -0.29 -0.53 -0.43 -0.57 -0.38 0.05 0.15 0.12 0.58 0.5 0.41 0.09 0.04 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.7 1.6 1.0 0.2 0.9 0 -0.4 0.2 -1.6 1.9 2.8 3.6 -1.9 -0.7 -0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.4 2.7 0 0 0 0 0 0 0 1.5 0 0 -0.6 1.1 1.4
Nucleus -1.7 -1.9 -4.4 -4.4 -4.0 -5.1 -3.6 -5.3 -1.0 -2.0 -1.9 -2.9 0.9 -2.0 -1.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 2.4 4.3 3.8


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cwc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available