Standard name
Description Anti-silencing protein; causes derepression of silent loci when overexpressed
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.34 0.36 0.54 0.52 0.42 0.38 0.35 0.34 0.4 0.35 0.28 0.44 0.42 0.43 0.53 0.29 0.16 0.25 0.18 0.25 0.23 0.41 0.33 0.41
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.1 0 0 0 0 0 0 0 0.1 0.15 0.24 0.34 0.25 0.28 0.23 0.12 0.12 0.1 0.14 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.64 0.63 0.42 0.43 0.35 0.63 0.67 0.66 0.69 0.73 0.72 0.64 0.16 0.15 0.06 0.17 0.21 0.12 0.16 0.15 0.18 0.14 0.17 0.15
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0.18 0.09 0 0.14 0.13 0.19 0 0.05 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0.05 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.07 0.13 0.05 0.07 0.06 0 0 0 0 0.05 0.05 0.05 0.12 0.08 0.09 0.17 0.2 0.13 0.2 0.17 0.1
Vac/Vac Membrane 0.12 0.22 0.05 0.06 0.1 0 0 0 0 0 0 0 0.2 0.25 0.21 0.21 0.4 0.19 0.07 0.09 0.11 0.06 0 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 3 1 2 5
Bud 0 4 4 3 2 9 7 15 8 7 2 6 0 0 1 0 0 0 1 3 0 0 2 4
Bud Neck 0 1 1 1 5 2 2 10 3 1 2 0 0 0 0 0 0 0 1 1 1 0 2 2
Bud Site 0 0 1 1 0 5 0 2 6 0 3 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 82 64 64 71 91 111 141 123 124 95 33 85 51 115 121 42 10 8 57 55 34 56 73 74
Endoplasmic Reticulum 3 1 4 1 3 0 0 2 0 0 3 1 0 3 4 10 4 1 6 1 1 2 5 1
Endosome 6 4 1 3 21 4 2 1 4 1 0 0 12 39 55 50 16 9 72 27 17 13 30 12
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 2 3
Mitochondria 156 112 50 58 76 185 274 241 216 197 84 125 19 40 14 25 13 4 49 33 26 19 37 26
Nucleus 2 5 4 3 2 6 9 9 11 5 1 6 22 24 9 20 8 6 5 10 6 1 1 1
Nuclear Periphery 3 4 0 1 0 0 2 3 0 1 0 0 0 2 0 1 0 0 11 5 4 0 1 0
Nucleolus 2 0 0 0 0 1 2 0 5 0 0 1 9 4 3 6 3 2 2 3 0 1 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 2 2 2 0 0 0 1 0 0 0 0 0
SpindlePole 5 3 3 10 27 15 27 21 14 3 0 6 6 12 12 17 5 3 51 43 19 27 37 18
Vac/Vac Membrane 30 39 6 8 22 5 9 15 6 12 1 6 24 66 48 31 25 6 21 20 16 8 9 20
Unique Cell Count 244 177 119 136 215 293 407 367 312 269 116 195 122 265 228 147 63 32 309 221 149 139 219 181
Labelled Cell Count 290 237 138 160 249 343 475 444 398 322 129 240 145 307 269 203 84 39 309 221 149 139 219 181


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 3.1 3.3 3.4 3.3 3.0 2.6 2.9 2.8 2.5 2.6 2.6 4.4 4.1 4.2 5.1 5.2 5.8 4.4 4.3 4.8
Std Deviation (1e-4) 0.5 0.6 0.8 1.0 1.0 1.2 0.8 0.8 1.3 0.8 0.7 1.2 1.0 1.0 1.2 1.8 1.2 0.8 2.2 1.8 1.7
Intensity Change (Log2) – – – 0.04 0.03 -0.14 -0.33 -0.17 -0.23 -0.41 -0.31 -0.35 0.42 0.33 0.35 0.64 0.65 0.82 0.44 0.38 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 –
Bud Neck – – – – – – – – – – – – 0 0 –
Bud Site – – – – – – – – – – – – 0 0 –
Cell Periphery – – – – – – – – – – – – 0 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 –
Endosome – – – – – – – – – – – – 0 0 –
Golgi – – – – – – – – – – – – 0 0 –
Mitochondria – – – – – – – – – – – – 0 0 –
Nuclear Periphery – – – – – – – – – – – – 0 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 –
Peroxisomes – – – – – – – – – – – – 0 0 –
SpindlePole – – – – – – – – – – – – 0 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 –
Cytoplasm – – – – – – – – – – – – 0 0 –
Nucleus – – – – – – – – – – – – 0 0 –
Vacuole – – – – – – – – – – – – 3.8 5.9 –