Standard name
Human Ortholog
Description Anti-silencing protein; causes derepression of silent loci when overexpressed

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.34 0.36 0.54 0.52 0.42 0.38 0.35 0.34 0.4 0.35 0.28 0.44 0.42 0.43 0.53 0.29 0.16 0.25 0.18 0.25 0.23 0.41 0.33 0.41
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.1 0 0 0 0 0 0 0 0.1 0.15 0.24 0.34 0.25 0.28 0.23 0.12 0.12 0.1 0.14 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.64 0.63 0.42 0.43 0.35 0.63 0.67 0.66 0.69 0.73 0.72 0.64 0.16 0.15 0.06 0.17 0.21 0.12 0.16 0.15 0.18 0.14 0.17 0.15
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0.18 0.09 0 0.14 0.13 0.19 0 0.05 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0.05 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.07 0.13 0.05 0.07 0.06 0 0 0 0 0.05 0.05 0.05 0.12 0.08 0.09 0.17 0.2 0.13 0.2 0.17 0.1
Vac/Vac Membrane 0.12 0.22 0.05 0.06 0.1 0 0 0 0 0 0 0 0.2 0.25 0.21 0.21 0.4 0.19 0.07 0.09 0.11 0.06 0 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 3 1 2 5
Bud 0 4 4 3 2 9 7 15 8 7 2 6 0 0 1 0 0 0 1 3 0 0 2 4
Bud Neck 0 1 1 1 5 2 2 10 3 1 2 0 0 0 0 0 0 0 1 1 1 0 2 2
Bud Site 0 0 1 1 0 5 0 2 6 0 3 4 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 82 64 64 71 91 111 141 123 124 95 33 85 51 115 121 42 10 8 57 55 34 56 73 74
Endoplasmic Reticulum 3 1 4 1 3 0 0 2 0 0 3 1 0 3 4 10 4 1 6 1 1 2 5 1
Endosome 6 4 1 3 21 4 2 1 4 1 0 0 12 39 55 50 16 9 72 27 17 13 30 12
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 2 3
Mitochondria 156 112 50 58 76 185 274 241 216 197 84 125 19 40 14 25 13 4 49 33 26 19 37 26
Nucleus 2 5 4 3 2 6 9 9 11 5 1 6 22 24 9 20 8 6 5 10 6 1 1 1
Nuclear Periphery 3 4 0 1 0 0 2 3 0 1 0 0 0 2 0 1 0 0 11 5 4 0 1 0
Nucleolus 2 0 0 0 0 1 2 0 5 0 0 1 9 4 3 6 3 2 2 3 0 1 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 2 2 2 0 0 0 1 0 0 0 0 0
SpindlePole 5 3 3 10 27 15 27 21 14 3 0 6 6 12 12 17 5 3 51 43 19 27 37 18
Vac/Vac Membrane 30 39 6 8 22 5 9 15 6 12 1 6 24 66 48 31 25 6 21 20 16 8 9 20
Unique Cell Count 244 177 119 136 215 293 407 367 312 269 116 195 122 265 228 147 63 32 309 221 149 139 219 181
Labelled Cell Count 290 237 138 160 249 343 475 444 398 322 129 240 145 307 269 203 84 39 309 221 149 139 219 181


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 3.1 3.3 3.4 3.3 3.0 2.6 2.9 2.8 2.5 2.6 2.6 4.4 4.1 4.2 5.1 5.2 5.8 4.4 4.3 4.8
Std Deviation (1e-4) 0.5 0.6 0.8 1.0 1.0 1.2 0.8 0.8 1.3 0.8 0.7 1.2 1.0 1.0 1.2 1.8 1.2 0.8 2.2 1.8 1.7
Intensity Change (Log2) 0.04 0.03 -0.14 -0.33 -0.17 -0.23 -0.41 -0.31 -0.35 0.42 0.33 0.35 0.64 0.65 0.82 0.44 0.38 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0
Bud Neck 0 0
Bud Site 0 0
Cell Periphery 0 0
Cyto
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nuclear Periphery 0 0
Nuc
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac
Cortical Patches 0 0
Cytoplasm 0 0
Nucleus 0 0
Vacuole 3.8 5.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2061 2.6226 2.4179 2.2133 1.4821 2.1055 -0.2476 0.2246 0.2212 -0.4448 -0.308 -0.2733 1.2405 1.4102 1.274 0.867 1.227 0.8977
Actin 0.0366 0.002 0.0124 0.0064 0.0012 0.0018 0.0214 0.0013 0.0382 0.0034 0.0017 0.0029 0.0742 0.0022 0.0269 0.0035 0.0215 0.0051
Bud 0.0005 0.0002 0.0002 0.0004 0.0001 0.0001 0.0005 0.0001 0.0006 0.0115 0 0.0001 0.0007 0.0001 0.0002 0.0001 0.0002 0.0002
Bud Neck 0.0022 0.0054 0.0028 0.012 0.0083 0.0087 0.0011 0.0007 0.0019 0.0013 0.0009 0.0038 0.0022 0.0008 0.0015 0.0011 0.004 0.0069
Bud Periphery 0.0007 0.0003 0.0003 0.0006 0.0001 0.0002 0.0008 0.0001 0.0008 0.0036 0 0.0002 0.0014 0.0001 0.0002 0.0001 0.0005 0.0006
Bud Site 0.0097 0.0075 0.0034 0.0074 0.0003 0.0007 0.0015 0.0017 0.0117 0.0017 0.0001 0.0003 0.0035 0.0024 0.002 0.0005 0.0006 0.0007
Cell Periphery 0.0003 0.0034 0.0002 0.0005 0.0001 0.0003 0.0003 0.0002 0.0004 0.001 0.0001 0.0003 0.0004 0.0001 0.0001 0.0001 0.0001 0.0003
Cytoplasm 0.0826 0.0956 0.0732 0.0262 0.0546 0.0865 0.1027 0.1173 0.0535 0.0725 0.0896 0.076 0.0745 0.0909 0.0752 0.089 0.1 0.1063
Cytoplasmic Foci 0.0689 0.0248 0.0294 0.0431 0.0455 0.0408 0.0584 0.0183 0.0396 0.0338 0.0235 0.0532 0.0811 0.1029 0.0282 0.0416 0.0464 0.036
Eisosomes 0.0006 0.0002 0.0002 0.0001 0.0002 0.0002 0.0005 0.0002 0.0013 0.0004 0.0003 0.0002 0.001 0.0001 0.0002 0.0001 0.0004 0.0002
Endoplasmic Reticulum 0.0097 0.0175 0.0119 0.0056 0.0062 0.0081 0.0181 0.0198 0.0121 0.0086 0.0193 0.0185 0.0142 0.0173 0.0145 0.0122 0.0139 0.0115
Endosome 0.0286 0.0262 0.0211 0.0335 0.0284 0.0242 0.0363 0.0276 0.0511 0.0119 0.0343 0.0448 0.0518 0.0464 0.0317 0.0462 0.062 0.0256
Golgi 0.0054 0.0017 0.0031 0.0061 0.0016 0.0015 0.0047 0.002 0.0084 0.0022 0.0007 0.0025 0.0105 0.0034 0.0041 0.0037 0.0066 0.0035
Lipid Particles 0.101 0.0252 0.0479 0.0159 0.0365 0.0211 0.0177 0.012 0.0394 0.036 0.0122 0.0495 0.0451 0.0407 0.0332 0.0535 0.0683 0.0309
Mitochondria 0.0039 0.0008 0.0011 0.0026 0.0012 0.0012 0.0046 0.0321 0.0235 0.0056 0.0006 0.0025 0.0053 0.0011 0.0016 0.0017 0.0048 0.0042
None 0.2329 0.228 0.1702 0.0814 0.1943 0.1262 0.3659 0.3263 0.1524 0.162 0.4875 0.2209 0.212 0.1516 0.1437 0.0982 0.151 0.1398
Nuclear Periphery 0.1291 0.1249 0.1413 0.1409 0.0991 0.0955 0.0874 0.1176 0.2174 0.0708 0.105 0.1291 0.1056 0.126 0.1936 0.1268 0.1478 0.0881
Nucleolus 0.0093 0.0195 0.0184 0.0556 0.0141 0.0307 0.0125 0.0191 0.0174 0.0148 0.0031 0.0287 0.0071 0.0089 0.0232 0.0103 0.0167 0.0251
Nucleus 0.0435 0.1878 0.1656 0.2389 0.1442 0.2358 0.0834 0.1402 0.1325 0.1903 0.0835 0.1393 0.0741 0.1018 0.1596 0.234 0.0714 0.1574
Peroxisomes 0.0191 0.0026 0.0068 0.0039 0.0047 0.0031 0.0016 0.0009 0.0084 0.0427 0.001 0.0031 0.0187 0.0057 0.0116 0.0041 0.0114 0.0039
Punctate Nuclear 0.2092 0.219 0.2857 0.3108 0.3558 0.3081 0.1742 0.1544 0.1817 0.3214 0.1252 0.2119 0.2091 0.2916 0.2422 0.2662 0.2664 0.3467
Vacuole 0.0045 0.0059 0.003 0.0045 0.0022 0.0038 0.0041 0.0059 0.0037 0.003 0.0098 0.0085 0.0043 0.0038 0.0035 0.0041 0.0029 0.0046
Vacuole Periphery 0.002 0.0016 0.0019 0.0036 0.0014 0.0016 0.0023 0.0022 0.004 0.0018 0.0013 0.0038 0.0034 0.0021 0.0029 0.0028 0.0031 0.0022

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.1239 6.8759 5.1022 9.3917 24.0249 28.6531 13.5884 8.7447 10.7993 19.5913
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Anti-silencing protein; causes derepression of silent loci when overexpressed
Localization
Cell Percentages nucleus (28%), cytoplasm (5%), mixed (36%)
Cell Cycle Regulation Yes
cytoplasm - T/G1 (0.00019)
nucleus - S/G2 (9e-05)
Subcompartmental Group N/A

Asf2

Asf2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Asf2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available