Standard name | |
---|---|
Human Ortholog | |
Description | Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 2 | 1 | 6 | 14 | 6 | 7 | 5 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 |
Bud | 2 | 0 | 0 | 2 | 0 | 3 | 10 | 3 | 6 | 14 | 11 | 17 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 12 | 3 | 18 | 8 | 36 | 36 | 23 | 37 | 27 | 26 | 14 | 21 | 17 | 3 | 10 | 5 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 134 | 177 | 270 | 277 | 406 | 375 | 419 | 360 | 273 | 337 | 233 | 273 | 475 | 612 | 562 | 156 | 244 | 181 | 127 | 170 | 263 | 301 | 300 | 425 |
Endoplasmic Reticulum | 0 | 4 | 19 | 4 | 10 | 13 | 45 | 39 | 22 | 24 | 13 | 11 | 11 | 13 | 8 | 3 | 5 | 3 | 1 | 2 | 13 | 1 | 2 | 10 |
Endosome | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 5 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 |
Golgi | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 |
Mitochondria | 2 | 0 | 3 | 14 | 40 | 77 | 219 | 150 | 121 | 238 | 174 | 198 | 0 | 0 | 0 | 0 | 4 | 3 | 0 | 0 | 3 | 0 | 1 | 0 |
Nucleus | 0 | 1 | 0 | 0 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 2 | 1 | 0 | 2 | 0 | 1 | 0 | 4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 2 | 2 | 9 | 10 | 23 | 36 | 22 | 24 | 18 | 30 | 1 | 0 | 3 | 4 | 7 | 17 | 0 | 2 | 0 | 5 | 4 | 4 |
Unique Cell Count | 135 | 184 | 292 | 291 | 445 | 440 | 633 | 547 | 402 | 548 | 402 | 456 | 483 | 621 | 571 | 162 | 255 | 202 | 134 | 179 | 294 | 314 | 315 | 460 |
Labelled Cell Count | 139 | 184 | 307 | 305 | 488 | 496 | 770 | 635 | 474 | 685 | 479 | 563 | 503 | 648 | 590 | 168 | 272 | 212 | 134 | 179 | 294 | 314 | 315 | 460 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.3 | 16.1 | 13.3 | 12.1 | 10.9 | 9.4 | 8.6 | 8.5 | 8.0 | 7.6 | 7.2 | 7.1 | 15.3 | 14.9 | 14.3 | 22.0 | 24.5 | 23.3 | 15.8 | 15.7 | 16.3 |
Std Deviation (1e-4) | 2.1 | 2.9 | 2.1 | 1.7 | 1.5 | 1.6 | 1.6 | 1.7 | 1.6 | 1.7 | 1.4 | 1.5 | 2.8 | 2.7 | 2.6 | 7.8 | 8.1 | 7.8 | 2.6 | 2.9 | 3.2 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.29 | -0.51 | -0.63 | -0.64 | -0.73 | -0.81 | -0.9 | -0.91 | 0.2 | 0.16 | 0.1 | 0.73 | 0.88 | 0.81 | 0.24 | 0.24 | 0.29 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.3 | 0 | -1.9 | 1.0 | 1.5 | 1.0 | 1.6 | 1.5 | 1.0 | -0.9 | -0.5 | -0.9 | -1.3 | -0.1 | -1.0 |
Cytoplasm | 1.4 | -0.6 | -3.0 | -8.5 | -8.5 | -7.7 | -9.5 | -10.0 | -9.8 | 4.1 | 4.7 | 4.5 | 1.6 | 1.6 | -1.1 |
Endoplasmic Reticulum | -3.2 | -2.9 | -2.3 | 0.3 | 0.3 | -0.6 | -1.3 | -2.0 | -2.8 | -3.0 | -3.4 | -4.1 | -2.2 | -2.6 | -2.7 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.7 | 4.5 | 7.0 | 11.1 | 9.4 | 9.9 | 12.9 | 12.6 | 12.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 2.6 | 3.9 | 3.4 | 2.9 | 2.9 | 3.9 | 0 | 0 | 0 | 0 | 0 | 4.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.7598 | 14.1615 | 12.6967 | 12.322 | 10.8947 | 12.7188 | 9.1941 | 13.3073 | 12.2707 | 10.6878 | 11.3606 | 12.2369 | 6.8945 | 11.7274 | 9.8708 | 8.8211 | 9.9018 | 10.8437 |
Actin | 0.021 | 0.0002 | 0.0029 | 0.0007 | 0.0378 | 0.0024 | 0.0188 | 0.0004 | 0.0042 | 0.0189 | 0.0011 | 0.0007 | 0.0296 | 0.0011 | 0.0071 | 0.0225 | 0.0004 | 0.0002 |
Bud | 0.0052 | 0.0002 | 0.006 | 0.0004 | 0.0005 | 0.0005 | 0.0008 | 0.0002 | 0.0002 | 0.0014 | 0.0002 | 0.0002 | 0.0019 | 0.0002 | 0.0004 | 0.0012 | 0.0004 | 0.0002 |
Bud Neck | 0.0012 | 0.0002 | 0.0007 | 0.0007 | 0.0007 | 0.0013 | 0.0037 | 0.0002 | 0.0004 | 0.0013 | 0.0004 | 0.001 | 0.0028 | 0.0001 | 0.0005 | 0.0006 | 0.0005 | 0.0009 |
Bud Periphery | 0.0022 | 0.0001 | 0.0025 | 0.0001 | 0.0003 | 0.0003 | 0.0008 | 0.0001 | 0.0001 | 0.0013 | 0.0001 | 0.0001 | 0.0015 | 0.0001 | 0.0001 | 0.0014 | 0.0001 | 0 |
Bud Site | 0.0054 | 0.0004 | 0.0023 | 0.0004 | 0.0005 | 0.0001 | 0.0019 | 0.0005 | 0.0003 | 0.0026 | 0.0003 | 0 | 0.0075 | 0.0003 | 0.0007 | 0.0034 | 0.0002 | 0 |
Cell Periphery | 0.0006 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 |
Cytoplasm | 0.8236 | 0.9542 | 0.8907 | 0.8128 | 0.8098 | 0.892 | 0.7937 | 0.9568 | 0.9091 | 0.7762 | 0.8757 | 0.9132 | 0.7419 | 0.9571 | 0.8792 | 0.7851 | 0.8635 | 0.9484 |
Cytoplasmic Foci | 0.0234 | 0.0079 | 0.0131 | 0.0215 | 0.0157 | 0.0123 | 0.017 | 0.0058 | 0.0105 | 0.0236 | 0.0216 | 0.0103 | 0.0328 | 0.0054 | 0.0135 | 0.0315 | 0.0133 | 0.0088 |
Eisosomes | 0.0005 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 |
Endoplasmic Reticulum | 0.0293 | 0.0106 | 0.0214 | 0.0074 | 0.0159 | 0.0274 | 0.0412 | 0.0138 | 0.0087 | 0.0144 | 0.0071 | 0.0357 | 0.0192 | 0.0104 | 0.0115 | 0.0122 | 0.0101 | 0.0116 |
Endosome | 0.0432 | 0.0192 | 0.0365 | 0.116 | 0.0752 | 0.0337 | 0.0519 | 0.0125 | 0.0322 | 0.0797 | 0.0634 | 0.0256 | 0.0648 | 0.0126 | 0.0431 | 0.0531 | 0.0786 | 0.0219 |
Golgi | 0.0086 | 0.0015 | 0.004 | 0.0061 | 0.0073 | 0.0057 | 0.017 | 0.0021 | 0.0021 | 0.0121 | 0.0066 | 0.0041 | 0.0254 | 0.0022 | 0.013 | 0.0294 | 0.0173 | 0.0019 |
Lipid Particles | 0.0052 | 0 | 0.0009 | 0.0001 | 0.0007 | 0.0006 | 0.0056 | 0.0001 | 0.0029 | 0.0016 | 0.0058 | 0.0001 | 0.0149 | 0.0004 | 0.0017 | 0.0025 | 0.0025 | 0 |
Mitochondria | 0.0056 | 0.0004 | 0.0019 | 0.0011 | 0.0048 | 0.0075 | 0.0135 | 0.0005 | 0.0078 | 0.0184 | 0.0045 | 0.0015 | 0.0136 | 0.0025 | 0.0132 | 0.0276 | 0.0015 | 0.0012 |
None | 0.0046 | 0.0005 | 0.0013 | 0.0026 | 0.0006 | 0.0031 | 0.0036 | 0.0005 | 0.001 | 0.0017 | 0.0008 | 0.0005 | 0.0029 | 0.0022 | 0.001 | 0.0006 | 0.0003 | 0.0003 |
Nuclear Periphery | 0.0027 | 0.0004 | 0.0036 | 0.0043 | 0.0017 | 0.0067 | 0.0072 | 0.0008 | 0.0021 | 0.0075 | 0.0017 | 0.0017 | 0.0115 | 0.001 | 0.0033 | 0.0015 | 0.0025 | 0.0005 |
Nucleolus | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 |
Nucleus | 0.0015 | 0.0005 | 0.0015 | 0.0067 | 0.0013 | 0.001 | 0.0041 | 0.0009 | 0.001 | 0.0034 | 0.0012 | 0.001 | 0.0072 | 0.001 | 0.0012 | 0.001 | 0.0009 | 0.0007 |
Peroxisomes | 0.0045 | 0 | 0.0002 | 0.0001 | 0.0028 | 0.0002 | 0.0026 | 0 | 0.0043 | 0.0028 | 0.0036 | 0 | 0.0073 | 0.0001 | 0.0009 | 0.0061 | 0.0003 | 0 |
Punctate Nuclear | 0.0028 | 0 | 0.0006 | 0.0009 | 0.0001 | 0.0003 | 0.004 | 0.0001 | 0.0006 | 0.0005 | 0.0008 | 0.0001 | 0.0022 | 0.0004 | 0.0011 | 0.0003 | 0.0003 | 0 |
Vacuole | 0.0069 | 0.0028 | 0.0077 | 0.0138 | 0.0207 | 0.0029 | 0.0093 | 0.0039 | 0.01 | 0.0242 | 0.0033 | 0.0028 | 0.0088 | 0.0024 | 0.0067 | 0.0148 | 0.0045 | 0.0022 |
Vacuole Periphery | 0.0017 | 0.0007 | 0.0018 | 0.004 | 0.0032 | 0.0017 | 0.0025 | 0.0006 | 0.0025 | 0.0077 | 0.0017 | 0.001 | 0.003 | 0.0005 | 0.0018 | 0.0046 | 0.0026 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 92.4268 | 63.8845 | 77.1122 | 64.8462 | 69.1833 | 68.5675 | 55.3351 | 65.9757 | 58.7128 | 58.8145 |
Translational Efficiency | 1.1734 | 1.5563 | 1.1825 | 1.4838 | 1.2994 | 1.2842 | 1.3892 | 1.102 | 1.2282 | 1.2417 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1931 | 640 | 1035 | 1137 | 1267 | 1434 | 376 | 1259 | 3198 | 2074 | 1411 | 2396 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1278.24 | 1307.60 | 1553.82 | 1886.95 | 1145.42 | 1314.65 | 1673.71 | 1990.34 | 1225.62 | 1312.47 | 1585.77 | 1941.28 |
Standard Deviation | 170.58 | 221.16 | 234.20 | 325.37 | 188.41 | 187.22 | 225.76 | 370.98 | 189.35 | 198.34 | 237.96 | 353.86 |
Intensity Change Log 2 | — | 0.032763 | 0.281661 | 0.561897 | — | 0.198802 | 0.547173 | 0.797138 | — | 0.113618 | 0.413243 | 0.677861 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000215 | 0.000382 | 0.000487 | 0.000768 | 0.000239 | 0.000374 | 0.000723 | 0.000568 | 0.000224 | 0.000376 | 0.000550 | 0.000663 |
Bud Neck | 0.000280 | 0.000447 | 0.002476 | 0.001826 | 0.000300 | 0.000453 | 0.004265 | 0.001093 | 0.000288 | 0.000452 | 0.002952 | 0.001440 |
Bud Site | 0.016592 | 0.029357 | 0.024131 | 0.041007 | 0.015081 | 0.022760 | 0.027855 | 0.018995 | 0.015993 | 0.024796 | 0.025124 | 0.029441 |
Cell Periphery | 0.000232 | 0.000297 | 0.000364 | 0.010288 | 0.000250 | 0.000222 | 0.000310 | 0.006574 | 0.000239 | 0.000245 | 0.000350 | 0.008337 |
Cytoplasm | 0.789313* | 0.836120* | 0.741262* | 0.769364* | 0.812172* | 0.803694* | 0.501897* | 0.870866* | 0.798369* | 0.813700* | 0.677477* | 0.822699* |
Cytoplasmic Foci | 0.133268 | 0.084193 | 0.070844 | 0.022118 | 0.098597 | 0.120408 | 0.095857 | 0.011313 | 0.119532 | 0.109233 | 0.077509 | 0.016440 |
Eisosomes | 0.000005 | 0.000003 | 0.000005 | 0.000006 | 0.000003 | 0.000005 | 0.000012 | 0.000006 | 0.000005 | 0.000004 | 0.000007 | 0.000006 |
Endoplasmic Reticulum | 0.003090 | 0.000948 | 0.002358 | 0.001824 | 0.009270 | 0.001990 | 0.004782 | 0.000585 | 0.005538 | 0.001669 | 0.003004 | 0.001173 |
Endosome | 0.005372 | 0.004912 | 0.015188 | 0.003746 | 0.006350 | 0.006900 | 0.029197 | 0.002332 | 0.005760 | 0.006286 | 0.018921 | 0.003003 |
Golgi | 0.018637 | 0.013293 | 0.008674 | 0.036354 | 0.010758 | 0.015364 | 0.008556 | 0.015142 | 0.015516 | 0.014725 | 0.008642 | 0.025208 |
Lipid Particles | 0.000935 | 0.001000 | 0.005523 | 0.017753 | 0.000641 | 0.000901 | 0.006124 | 0.012756 | 0.000818 | 0.000932 | 0.005684 | 0.015127 |
Mitochondria | 0.000686 | 0.001204 | 0.000043 | 0.000179 | 0.000648 | 0.000417 | 0.000024 | 0.000935 | 0.000671 | 0.000660 | 0.000038 | 0.000577 |
Mitotic Spindle | 0.000978 | 0.002963 | 0.009413 | 0.002109 | 0.000649 | 0.001976 | 0.005979 | 0.000983 | 0.000848 | 0.002280 | 0.008498 | 0.001517 |
None | 0.000320 | 0.000272 | 0.000175 | 0.000223 | 0.000535 | 0.000197 | 0.000067 | 0.000438 | 0.000405 | 0.000220 | 0.000146 | 0.000336 |
Nuclear Periphery | 0.000015 | 0.000015 | 0.000169 | 0.000063 | 0.000064 | 0.000013 | 0.000182 | 0.000061 | 0.000035 | 0.000014 | 0.000172 | 0.000062 |
Nuclear Periphery Foci | 0.000332 | 0.000755 | 0.004082 | 0.003700 | 0.001122 | 0.000393 | 0.004673 | 0.003092 | 0.000645 | 0.000505 | 0.004239 | 0.003381 |
Nucleolus | 0.000054 | 0.000028 | 0.000064 | 0.000069 | 0.000047 | 0.000024 | 0.000091 | 0.000085 | 0.000051 | 0.000025 | 0.000071 | 0.000077 |
Nucleus | 0.001561 | 0.002061 | 0.004745 | 0.002816 | 0.002756 | 0.001606 | 0.005390 | 0.004375 | 0.002034 | 0.001747 | 0.004916 | 0.003635 |
Peroxisomes | 0.000217 | 0.000393 | 0.000473 | 0.000278 | 0.000168 | 0.000478 | 0.000974 | 0.000267 | 0.000197 | 0.000452 | 0.000606 | 0.000272 |
Vacuole | 0.013219 | 0.014824 | 0.102107 | 0.064302 | 0.023439 | 0.015881 | 0.293677* | 0.043003 | 0.017268 | 0.015555 | 0.153156 | 0.053110 |
Vacuole Periphery | 0.014680 | 0.006532 | 0.007418 | 0.021207 | 0.016912 | 0.005941 | 0.009365 | 0.006531 | 0.015564 | 0.006124 | 0.007937 | 0.013495 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.34 | -5.08 | -7.46 | -4.22 | -3.20 | -2.51 | -4.80 | -1.74 | -1.03 | 0.87 | -3.94 | -6.77 | -4.30 | -2.80 | -0.93 |
Bud Neck | -2.91 | -12.74 | -10.59 | -9.18 | 3.43 | -3.97 | -9.07 | -9.54 | -7.46 | 7.13 | -4.86 | -15.47 | -14.00 | -11.79 | 8.26 |
Bud Site | -4.29 | -4.68 | -9.08 | -3.15 | -5.55 | -4.90 | -5.28 | -2.41 | 1.94 | 3.37 | -6.34 | -6.80 | -8.67 | -2.58 | -2.11 |
Cell Periphery | -2.30 | -4.81 | -11.22 | -11.15 | -11.07 | 1.10 | -2.09 | -7.95 | -7.98 | -7.86 | -0.35 | -5.15 | -13.56 | -13.54 | -13.36 |
Cytoplasm | -4.59 | 5.67 | 2.38 | 6.01 | -3.06 | 0.92 | 17.32 | -6.32 | -7.60 | -20.84 | -2.34 | 13.93 | -3.30 | -0.82 | -15.78 |
Cytoplasmic Foci | 7.75 | 12.87 | 27.50 | 12.00 | 15.38 | -3.78 | 0.58 | 21.53 | 25.59 | 15.73 | 2.40 | 11.07 | 35.30 | 27.47 | 22.64 |
Eisosomes | 1.33 | 0.22 | -0.39 | -3.52 | -1.67 | -2.72 | -8.06 | -1.23 | -0.54 | 2.29 | 0.06 | -2.60 | -1.04 | -1.23 | 0.63 |
Endoplasmic Reticulum | 3.84 | 1.27 | 2.01 | -2.01 | 0.84 | 4.68 | 2.77 | 5.68 | 4.42 | 4.70 | 5.40 | 3.47 | 6.18 | 1.80 | 4.10 |
Endosome | 0.76 | -9.72 | 4.31 | 1.99 | 11.35 | -0.73 | -10.56 | 6.47 | 6.53 | 12.60 | -1.05 | -13.72 | 7.87 | 6.66 | 16.66 |
Golgi | 4.29 | 12.21 | -6.94 | -8.66 | -11.33 | -3.97 | 2.46 | -2.49 | 0.28 | -3.70 | 0.90 | 11.22 | -6.24 | -6.23 | -11.18 |
Lipid Particles | -0.37 | -10.51 | -11.82 | -11.71 | -7.85 | -2.38 | -7.25 | -11.26 | -10.98 | -4.83 | -1.18 | -12.86 | -16.22 | -16.05 | -9.35 |
Mitochondria | -0.62 | 1.95 | 1.53 | 1.34 | -3.60 | 0.53 | 1.58 | -0.41 | -0.96 | -1.87 | 0.04 | 2.49 | 0.33 | 0.29 | -2.11 |
Mitotic Spindle | -1.74 | -5.80 | -1.97 | 0.70 | 4.98 | -3.27 | -3.29 | -1.55 | 2.22 | 3.06 | -2.91 | -6.70 | -2.04 | 1.51 | 6.12 |
None | 0.49 | 1.41 | 1.04 | 0.83 | -1.42 | 4.10 | 6.71 | 0.98 | -2.92 | -5.31 | 2.52 | 3.77 | 0.96 | -2.25 | -4.48 |
Nuclear Periphery | -0.00 | -3.04 | -6.60 | -6.91 | 2.10 | 2.07 | -3.55 | 0.08 | -3.62 | 4.43 | 2.04 | -3.58 | -2.29 | -6.32 | 2.91 |
Nuclear Periphery Foci | -4.43 | -9.77 | -16.05 | -12.91 | 1.40 | 4.89 | -6.06 | -5.30 | -7.65 | 2.38 | 1.94 | -11.11 | -12.47 | -13.39 | 2.60 |
Nucleolus | 0.96 | -0.37 | -0.41 | -7.69 | -0.14 | 1.72 | -3.23 | -2.03 | -4.13 | 0.58 | 1.44 | -1.16 | -1.21 | -6.36 | -0.26 |
Nucleus | -1.38 | -8.09 | -5.43 | -3.02 | 4.72 | 4.00 | -5.41 | -4.81 | -8.52 | 1.68 | 1.38 | -9.29 | -7.49 | -9.39 | 3.61 |
Peroxisomes | -2.50 | -6.31 | -2.57 | 1.58 | 4.57 | -6.05 | -7.34 | -2.62 | 3.57 | 6.23 | -6.12 | -9.73 | -3.27 | 4.00 | 7.43 |
Vacuole | -0.85 | -16.97 | -16.09 | -13.71 | 6.52 | 3.22 | -17.73 | -7.11 | -10.94 | 16.15 | 1.13 | -22.71 | -17.29 | -17.80 | 15.80 |
Vacuole Periphery | 4.53 | 5.11 | -2.64 | -6.06 | -6.46 | 6.15 | 4.04 | 5.59 | -0.37 | 1.55 | 8.16 | 6.64 | 1.72 | -5.81 | -4.47 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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