Standard name
Description Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.06 0.07 0.06 0.07 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.96 0.92 0.95 0.91 0.85 0.66 0.66 0.68 0.61 0.58 0.6 0.98 0.99 0.98 0.96 0.96 0.9 0.95 0.95 0.9 0.96 0.96 0.93
Endoplasmic Reticulum 0 0 0.07 0 0 0 0.07 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0.09 0.17 0.35 0.27 0.3 0.43 0.43 0.43 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0.05 0 0 0.07 0 0 0 0 0 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 2 1 6 14 6 7 5 1 3 0 0 0 0 0 0 0 0 3 0 0 0
Bud 2 0 0 2 0 3 10 3 6 14 11 17 1 1 0 0 0 0 1 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 12 3 18 8 36 36 23 37 27 26 14 21 17 3 10 5 0 0 1 0 0 0
Cytoplasm 134 177 270 277 406 375 419 360 273 337 233 273 475 612 562 156 244 181 127 170 263 301 300 425
Endoplasmic Reticulum 0 4 19 4 10 13 45 39 22 24 13 11 11 13 8 3 5 3 1 2 13 1 2 10
Endosome 0 0 0 0 1 2 1 0 0 5 1 2 1 0 0 0 0 0 0 0 0 1 0 2
Golgi 1 0 1 1 1 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 0 0 0
Mitochondria 2 0 3 14 40 77 219 150 121 238 174 198 0 0 0 0 4 3 0 0 3 0 1 0
Nucleus 0 1 0 0 2 1 1 2 0 0 0 1 0 1 0 2 2 1 0 2 0 1 0 4
Nuclear Periphery 0 0 0 0 0 0 1 2 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 2 2 9 10 23 36 22 24 18 30 1 0 3 4 7 17 0 2 0 5 4 4
Unique Cell Count 135 184 292 291 445 440 633 547 402 548 402 456 483 621 571 162 255 202 134 179 294 314 315 460
Labelled Cell Count 139 184 307 305 488 496 770 635 474 685 479 563 503 648 590 168 272 212 134 179 294 314 315 460


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.3 16.1 13.3 12.1 10.9 9.4 8.6 8.5 8.0 7.6 7.2 7.1 15.3 14.9 14.3 22.0 24.5 23.3 15.8 15.7 16.3
Std Deviation (1e-4) 2.1 2.9 2.1 1.7 1.5 1.6 1.6 1.7 1.6 1.7 1.4 1.5 2.8 2.7 2.6 7.8 8.1 7.8 2.6 2.9 3.2
Intensity Change (Log2) -0.14 -0.29 -0.51 -0.63 -0.64 -0.73 -0.81 -0.9 -0.91 0.2 0.16 0.1 0.73 0.88 0.81 0.24 0.24 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 3.3 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.3 0 -1.9 1.0 1.5 1.0 1.6 1.5 1.0 -0.9 -0.5 -0.9 -1.3 -0.1 -1.0
Cytoplasm 1.4 -0.6 -3.0 -8.5 -8.5 -7.7 -9.5 -10.0 -9.8 4.1 4.7 4.5 1.6 1.6 -1.1
Endoplasmic Reticulum -3.2 -2.9 -2.3 0.3 0.3 -0.6 -1.3 -2.0 -2.8 -3.0 -3.4 -4.1 -2.2 -2.6 -2.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.7 4.5 7.0 11.1 9.4 9.9 12.9 12.6 12.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.6 3.9 3.4 2.9 2.9 3.9 0 0 0 0 0 4.4


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Npa3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available