Standard name
Description Forms dehydrodolichyl diphosphate syntase complex with RER2 or SRT1; tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY, suggesting that Nus1p may be involved in protein trafficking; mutations in human homolog NUS1 have been implicated in congenital scoliosis, neurological impairment, refractory epilepsy, hearing deficit, and visual impairment; human cis-prenyltransferase complex complements yeast null mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.21 0.14 0.18 0 0 0 0 0 0 0 0 0.06 0 0 0 0.06
Bud 0 0 0.09 0.09 0.06 0.07 0 0.08 0.05 0 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.06 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0.08 0.09 0.07 0 0 0 0 0 0 0 0.05 0.06 0 0.9 0.18 0.55
Endoplasmic Reticulum 0.42 0.25 0.06 0 0 0 0 0 0 0 0 0 0.59 0.45 0 0 0
Endosome 0.05 0 0.11 0.17 0.08 0 0 0 0 0 0 0.28 0.06 0.07 0.09 0 0
Golgi 0.09 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0 0 0
Mitochondria 0.06 0.46 0.67 0.61 0.8 0.88 0.79 0.92 0.88 0.91 0.9 0.31 0 0.26 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.31 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08
Nucleolus 0.07 0 0 0 0.06 0.09 0.08 0.07 0.06 0.1 0.09 0 0 0 0 0.4 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.05 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0
Vac/Vac Membrane 0.25 0.06 0.16 0.11 0.12 0.12 0.19 0.1 0.15 0.16 0.19 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 30 17 34 7 2 6 2 1 0 0 0 6 7 0 0 0
Bud 0 6 11 16 14 17 10 18 12 8 12 0 1 0 0 0 0
Bud Neck 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 2 3 5 7 2 1 1
Cell Periphery 0 9 6 9 1 5 1 4 2 3 4 0 0 4 0 0 0
Cytoplasm 16 12 11 14 9 10 5 4 6 7 3 0 6 2 0 0 3
Endoplasmic Reticulum 34 36 7 8 2 0 1 2 2 2 1 0 61 105 0 0 0
Endosome 4 5 14 32 18 4 4 1 0 1 0 1 5 17 0 0 0
Golgi 7 2 2 6 0 1 1 1 0 1 0 0 8 17 0 0 0
Mitochondria 5 67 84 114 176 205 195 212 221 193 188 1 4 61 0 0 0
Nucleus 0 1 0 0 1 1 5 3 3 2 1 0 0 0 0 0 0
Nuclear Periphery 3 0 0 0 2 1 3 3 6 6 6 0 0 2 0 0 0
Nucleolus 6 0 1 0 14 20 19 16 14 21 18 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 3 1 10 8 6 1 4 1 0 2 0 0 0 0 0 0
Vac/Vac Membrane 20 9 20 20 27 28 46 22 37 35 40 0 5 7 0 0 0
Unique Cell Count 80 146 125 187 221 233 246 231 252 213 209 6 104 235 1 2 6
Labelled Cell Count 98 180 174 263 281 304 302 299 307 280 276 6 104 235 1 2 6


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.7 7.5 6.6 7.3 6.7 6.6 7.1 6.5 6.5 6.6 6.7
Std Deviation (1e-4) 9.4 1.4 1.5 1.5 1.6 1.3 1.4 1.7 1.3 1.4 1.5
Intensity Change (Log2) -0.19 -0.04 -0.18 -0.2 -0.09 -0.21 -0.21 -0.19 -0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.5 -0.5 -5.4 -6.7 -6.0 -6.7 -7.2 -6.9 -6.8
Bud 1.6 1.6 0.9 1.3 0 1.4 0.3 -0.2 0.7
Bud Neck 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0
Cell Periphery -0.5 -0.5 0 -2.0 0 -2.3 0 0 -2.1
Cytoplasm 0.2 -0.2 -1.7 -1.6 -2.9 -3.0 -2.7 -2.1 -3.1
Endoplasmic Reticulum -4.3 -5.4 -7.3 -8.0 -7.9 -7.5 -7.8 -7.2 -7.3
Endosome 2.5 3.9 1.8 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0
Mitochondria 3.5 2.7 6.7 8.9 6.8 9.9 9.0 9.3 9.1
Nucleus 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 3.1 3.6 3.4 3.2 2.9 3.9 3.6
Peroxisomes 0 0 0 0 0 0 0 0 0
SpindlePole 0 1.5 0 0 0 0 0 0 0
Vacuole 2.6 1.5 1.9 1.9 3.5 1.2 2.6 2.9 3.5


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nus1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available