Standard name
Human Ortholog
Description Protein involved in assembly of the RNA polymerase III (Pol III) complex; high copy suppressor of Pol III assembly mutation and psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; physically interacts with Pol III; proposed to bind single-stranded nucleic acids via its R3H domain

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.97 0.96 0.96 0.84 0.75 0.71 0.6 0.53 0.51 0.51 0.99 0.98 0.98 0.91 0.91 0.89 0.91 0.91 0.88 0.91 0.84 0.58
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0.05 0 0.06 0 0 0 0 0 0 0.05 0 0 0 0.05 0.05 0.06 0.13
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.28 0.27 0.34 0.47 0.56 0.59 0.65 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 0 1 1 1 0 0 2 1 2 4 0 1 1 1 4 2 1 0 0 0 2 9
Bud 2 1 3 2 2 2 4 8 7 3 7 7 6 5 5 0 0 0 2 0 0 0 1 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 2
Bud Site 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Cell Periphery 1 2 0 5 6 8 12 4 10 10 7 10 5 12 15 16 11 9 0 0 0 0 0 0
Cytoplasm 488 145 116 217 279 242 281 284 189 167 170 202 447 711 844 388 238 101 469 144 107 250 392 334
Endoplasmic Reticulum 0 1 2 4 3 11 22 19 13 20 9 15 3 6 5 14 13 4 19 2 5 12 30 72
Endosome 10 0 0 1 1 0 5 3 5 0 0 0 0 4 6 18 5 2 3 0 1 0 10 52
Golgi 0 0 1 0 0 1 0 0 0 0 1 0 1 2 0 3 0 1 0 0 0 1 8 22
Mitochondria 14 1 2 8 12 82 99 135 149 176 199 258 0 1 1 15 10 4 2 1 2 1 4 19
Nucleus 2 2 0 1 0 0 1 0 2 0 4 2 0 1 1 0 0 1 1 1 0 0 0 1
Nuclear Periphery 0 0 0 0 0 1 1 0 2 3 1 2 0 0 0 0 0 0 1 0 0 0 1 2
Nucleolus 1 0 0 0 0 0 0 0 1 0 0 0 1 0 2 1 2 2 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 2 2 1 0 0 0 3 16
Vac/Vac Membrane 15 0 0 4 4 5 8 10 6 10 12 16 0 1 4 14 10 1 3 6 2 1 7 24
Unique Cell Count 514 148 119 227 290 289 373 402 315 313 335 397 451 727 859 426 261 113 517 159 122 275 469 581
Labelled Cell Count 535 152 124 243 308 353 433 463 386 390 413 517 463 744 885 472 295 129 517 159 122 275 469 581


Cytoplasm, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.2 7.7 7.5 7.9 7.6 6.5 6.1 6.2 5.9 5.4 5.6 5.4 9.3 8.9 8.8 7.7 8.6 10.0 8.6 8.5 9.4
Std Deviation (1e-4) 0.9 1.1 1.0 1.0 1.3 1.0 0.9 1.2 1.1 1.1 1.2 1.0 1.2 1.1 1.0 1.0 1.3 1.4 1.2 1.4 1.4
Intensity Change (Log2) 0.06 0.01 -0.21 -0.32 -0.29 -0.35 -0.48 -0.43 -0.49 0.3 0.24 0.22 0.04 0.19 0.4 0.19 0.18 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.9 -0.6 -3.8 -5.3 -6.1 -7.6 -8.6 -9.1 -9.2 1.4 0.2 0.6 -2.3 -2.3 -2.5
Endoplasmic Reticulum 0 0 0 1.9 0 0 2.0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.1 5.8 6.9 8.9 10.3 10.9 12.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4908 4.9257 4.3481 3.5432 3.5871 4.5198 2.9397 4.6158 4.0849 3.5192 3.5119 4.1227 5.7172 7.319 6.372 6.4011 5.9392 6.6236
Actin 0.0118 0.0012 0.006 0.0184 0.0014 0.0118 0.0301 0.0016 0.0235 0.0218 0 0.0105 0.0211 0.0004 0.0083 0.0034 0.014 0.0054
Bud 0.0041 0.0035 0.0023 0.0025 0.0005 0.001 0.0008 0.0002 0.0012 0.0016 0 0.0002 0.0058 0.0094 0.0127 0.0048 0.0014 0.0033
Bud Neck 0.0006 0.0003 0.0018 0.0022 0.0006 0.0016 0.0016 0.0001 0.0023 0.0026 0.0002 0.0007 0.0016 0.0002 0.0009 0.0003 0.0005 0.0007
Bud Periphery 0.0033 0.0011 0.002 0.0009 0.0005 0.0007 0.0008 0.0001 0.0006 0.0036 0 0.0001 0.0016 0.0014 0.0043 0.0024 0.0008 0.0019
Bud Site 0.0017 0.0133 0.007 0.0024 0.0013 0.0002 0.0344 0.0007 0.0029 0.0097 0 0.0001 0.0056 0.0033 0.0097 0.0022 0.0008 0.0002
Cell Periphery 0.0015 0.001 0.0005 0.0003 0.0002 0.0001 0.0006 0.0002 0.0002 0.0018 0 0.0001 0.0007 0.0002 0.0007 0.0001 0.0002 0.0001
Cytoplasm 0.8415 0.9378 0.8915 0.7824 0.81 0.8906 0.5606 0.9368 0.8225 0.785 0.9717 0.9219 0.82 0.9542 0.8804 0.9288 0.8413 0.9255
Cytoplasmic Foci 0.0209 0.0085 0.0188 0.0444 0.019 0.0219 0.0882 0.0049 0.0537 0.0274 0.0058 0.0092 0.0235 0.0076 0.0176 0.0117 0.0231 0.0143
Eisosomes 0.0001 0 0.0001 0.0004 0.0001 0 0.0003 0 0.0001 0.0005 0 0.0003 0.0001 0 0 0 0.0002 0
Endoplasmic Reticulum 0.0193 0.0079 0.0139 0.0128 0.0167 0.0216 0.0233 0.0043 0.0084 0.0061 0.0057 0.0106 0.0157 0.0007 0.0112 0.0057 0.0054 0.0063
Endosome 0.0288 0.0131 0.0168 0.0472 0.0471 0.0252 0.0894 0.0056 0.0273 0.0583 0.0106 0.0136 0.033 0.0065 0.0139 0.0149 0.0138 0.0118
Golgi 0.0056 0.0012 0.0029 0.025 0.0055 0.0011 0.0284 0.0015 0.0143 0.0186 0.0002 0.0017 0.0074 0.0005 0.0087 0.0012 0.0064 0.0028
Lipid Particles 0.0048 0.0002 0.001 0.0215 0.0045 0.0003 0.0374 0.0001 0.005 0.003 0 0.0009 0.0022 0.0001 0.0007 0.0002 0.0075 0.0005
Mitochondria 0.0045 0.0006 0.0024 0.0039 0.0219 0.0015 0.0063 0.0003 0.005 0.0329 0.0001 0.0013 0.0026 0.0017 0.0094 0.0006 0.0547 0.0012
None 0.0316 0.0045 0.0202 0.0085 0.0267 0.0142 0.0438 0.0419 0.0093 0.0021 0.0028 0.0177 0.0375 0.0085 0.0067 0.0132 0.0126 0.0194
Nuclear Periphery 0.0026 0.0009 0.0021 0.0034 0.0057 0.0014 0.0095 0.0004 0.0045 0.001 0.0006 0.0008 0.0061 0.0002 0.0012 0.0019 0.0035 0.0005
Nucleolus 0.0008 0.0001 0.0003 0.0008 0.0043 0.0003 0.0006 0 0.0006 0.001 0 0.0001 0.0004 0.0016 0.0002 0.0001 0.0004 0.0001
Nucleus 0.0033 0.0013 0.002 0.0049 0.0052 0.0016 0.0026 0.0004 0.0027 0.0017 0.0004 0.0006 0.005 0.0013 0.0049 0.0017 0.0014 0.0009
Peroxisomes 0.0058 0.0001 0.0008 0.0047 0.0009 0.0003 0.0202 0 0.0031 0.001 0 0.0081 0.0016 0.0002 0.0054 0.0003 0.0058 0.0003
Punctate Nuclear 0.0011 0.0003 0.0014 0.0066 0.0215 0.001 0.0147 0.0001 0.0104 0.0008 0 0.0004 0.0048 0.0012 0.0007 0.0044 0.0029 0.0038
Vacuole 0.0052 0.0027 0.0056 0.0051 0.0044 0.0025 0.0043 0.0007 0.0015 0.0176 0.0015 0.0008 0.0028 0.0008 0.0019 0.0016 0.0024 0.0008
Vacuole Periphery 0.0012 0.0005 0.0007 0.0017 0.0019 0.0011 0.002 0.0001 0.0009 0.0017 0.0003 0.0004 0.0009 0.0002 0.0005 0.0004 0.001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.0842 30.8691 26.1024 28.9674 34.3597 39.3282 28.4898 31.5987 30.0477 33.1311
Translational Efficiency 1.4042 1.0443 0.943 0.9486 0.6683 0.8043 1.0558 0.8169 0.7487 0.9354

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1057 1358 2825 1350 662 863 2226 1945 1719 2221 5051 3295

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 810.14 913.77 1101.44 985.85 954.08 954.03 1099.60 1093.65 865.57 929.41 1100.63 1049.48
Standard Deviation 93.08 159.65 135.12 167.98 120.25 132.94 121.81 131.95 125.70 151.12 129.43 157.00
Intensity Change Log 2 0.173660 0.443148 0.283197 -0.000076 0.204797 0.196969 0.082310 0.319155 0.237206

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000270 0.000221 0.000186 0.000366 0.000452 0.000640 0.000330 0.001152 0.000340 0.000384 0.000250 0.000830
Bud Neck 0.002696 0.001874 0.001621 0.003579 0.001411 0.003907 0.001236 0.004981 0.002201 0.002664 0.001451 0.004407
Bud Site 0.020777 0.021355 0.009327 0.031991 0.018137 0.043603 0.011849 0.023645 0.019760 0.030000 0.010438 0.027064
Cell Periphery 0.000310 0.000119 0.000058 0.000083 0.000291 0.000158 0.000071 0.000088 0.000303 0.000134 0.000063 0.000086
Cytoplasm 0.579875* 0.701521* 0.854500* 0.668508* 0.603904* 0.548713* 0.793267* 0.589232* 0.589129* 0.642145* 0.827514* 0.621712*
Cytoplasmic Foci 0.286494* 0.159564 0.049099 0.091757 0.263220* 0.278966* 0.073809 0.074110 0.277531* 0.205959* 0.059989 0.081340
Eisosomes 0.000049 0.000013 0.000005 0.000012 0.000033 0.000041 0.000007 0.000015 0.000043 0.000023 0.000006 0.000014
Endoplasmic Reticulum 0.010789 0.003974 0.000615 0.000471 0.011114 0.002169 0.000684 0.000819 0.010914 0.003273 0.000646 0.000677
Endosome 0.009798 0.014792 0.003076 0.011197 0.018495 0.024763 0.006433 0.015767 0.013147 0.018666 0.004555 0.013895
Golgi 0.018761 0.008944 0.001141 0.004337 0.013214 0.017106 0.001913 0.002754 0.016625 0.012116 0.001481 0.003402
Lipid Particles 0.004807 0.002233 0.001495 0.001169 0.003539 0.001766 0.001592 0.001555 0.004319 0.002051 0.001538 0.001397
Mitochondria 0.015527 0.000830 0.000199 0.000874 0.003240 0.001960 0.000173 0.000965 0.010795 0.001269 0.000187 0.000928
Mitotic Spindle 0.003207 0.001088 0.002071 0.017612 0.001037 0.002263 0.003122 0.032481 0.002371 0.001544 0.002534 0.026389
None 0.002980 0.002717 0.000650 0.001408 0.002129 0.000292 0.000284 0.000672 0.002652 0.001774 0.000488 0.000974
Nuclear Periphery 0.000132 0.000185 0.000077 0.000274 0.000129 0.000050 0.000071 0.000643 0.000130 0.000132 0.000074 0.000492
Nuclear Periphery Foci 0.000621 0.001636 0.001454 0.004120 0.001515 0.000486 0.001474 0.001898 0.000965 0.001189 0.001462 0.002808
Nucleolus 0.000309 0.000450 0.000154 0.000516 0.000188 0.000228 0.000169 0.000605 0.000262 0.000364 0.000161 0.000569
Nucleus 0.012736 0.010404 0.022641 0.016974 0.007889 0.004326 0.012660 0.018783 0.010870 0.008042 0.018243 0.018041
Peroxisomes 0.001517 0.000892 0.000659 0.005178 0.000846 0.003542 0.000634 0.003926 0.001259 0.001922 0.000648 0.004439
Vacuole 0.014120 0.060151 0.050345 0.138423 0.035877 0.057884 0.089073 0.223478* 0.022499 0.059270 0.067412 0.188630
Vacuole Periphery 0.014226 0.007038 0.000629 0.001153 0.013340 0.007138 0.001148 0.002431 0.013885 0.007077 0.000858 0.001908

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.26 2.39 -2.04 -3.60 -3.60 -2.03 1.64 -0.99 -0.25 -1.47 -1.14 2.26 -1.42 -1.10 -1.96
Bud Neck 1.62 1.85 -1.55 -4.48 -4.88 -4.14 0.59 -5.89 -0.80 -6.13 -1.18 2.02 -4.37 -3.52 -7.38
Bud Site -0.26 7.56 -1.30 -1.05 -7.18 -8.67 4.80 -0.38 8.05 -4.48 -5.95 8.82 -0.75 4.90 -8.37
Cell Periphery 8.89 12.43 11.83 5.93 -1.17 4.56 9.01 7.78 4.11 0.19 9.71 15.31 13.04 5.60 -0.20
Cytoplasm -11.51 -31.11 -2.81 8.41 26.55 3.87 -15.11 2.83 -1.65 24.08 -6.24 -32.78 0.33 7.03 37.52
Cytoplasmic Foci 16.57 38.00 30.60 13.88 -7.96 -1.47 24.84 25.86 27.96 2.50 11.42 44.86 41.31 28.96 -4.34
Eisosomes 13.74 17.30 14.17 0.08 -12.18 -1.11 5.56 4.26 5.35 -7.50 6.16 15.45 12.43 5.03 -12.99
Endoplasmic Reticulum 5.00 8.27 8.53 5.99 1.51 5.16 6.26 6.31 3.58 0.72 7.14 10.37 10.55 6.84 1.36
Endosome -4.38 9.86 1.21 5.22 -7.52 -3.09 9.37 4.70 7.54 -5.35 -5.22 13.05 3.41 8.21 -9.14
Golgi 10.39 22.64 14.69 6.80 -1.51 -2.58 13.42 12.92 12.23 0.10 5.56 25.98 21.20 14.57 -1.42
Lipid Particles 6.42 8.54 9.06 3.71 1.40 4.31 5.26 5.30 0.92 1.12 7.72 10.02 10.03 2.99 1.50
Mitochondria 8.52 8.91 8.70 1.65 -1.49 0.94 2.47 2.10 2.52 -2.28 8.01 9.07 8.71 2.92 -2.67
Mitotic Spindle 2.45 0.77 -6.01 -7.13 -6.51 -1.86 -3.67 -9.68 -8.99 -8.22 1.39 -1.21 -10.96 -11.62 -10.62
None 0.39 6.37 4.15 2.44 -1.15 1.89 1.88 1.55 -3.51 -4.14 1.53 4.95 4.12 2.44 -2.09
Nuclear Periphery -0.89 1.65 -3.79 -2.04 -4.54 3.19 0.88 -3.46 -4.16 -3.68 -0.01 1.81 -4.44 -4.08 -4.86
Nuclear Periphery Foci -5.49 -6.43 -4.32 -2.77 -2.77 5.31 -1.10 -0.44 -3.59 0.42 -1.68 -4.96 -4.29 -3.58 -1.98
Nucleolus -1.16 1.46 -0.87 0.04 -2.20 -0.38 0.20 -2.78 -1.99 -2.99 -1.18 1.45 -2.30 -1.33 -3.73
Nucleus 2.19 -13.88 -6.32 -7.56 4.70 5.10 -7.94 -13.37 -20.28 -7.65 4.20 -15.32 -13.21 -16.31 -0.00
Peroxisomes 2.70 3.71 -4.00 -5.32 -5.72 -3.71 1.72 -5.35 0.78 -6.22 -2.09 4.10 -5.95 -3.39 -8.39
Vacuole -14.99 -24.73 -32.56 -22.99 -24.47 -5.06 -18.54 -36.17 -31.28 -23.84 -14.56 -29.48 -50.45 -38.94 -35.68
Vacuole Periphery 5.76 11.88 11.78 12.16 -0.77 3.42 7.23 6.88 8.05 -1.50 6.57 13.61 13.04 13.58 -2.01