Standard name
Description Methylene-fatty-acyl-phospholipid synthase; catalyzes the last two steps in phosphatidylcholine biosynthesis; also known as phospholipid methyltransferase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0 0 0
Cytoplasm 0.46 0.47 0.37 0.37 0.55 0.57 0.59 0.66 0.67 0.66 0.7 0.67 0.19 0.26 0.23 0.33 0.26 0.32 0.05 0 0 0 0 0
Endoplasmic Reticulum 0.34 0.49 0.5 0.6 0.4 0.37 0.31 0.28 0.24 0.24 0.23 0.22 0.72 0.68 0.66 0 0 0 0.6 0.7 0.69 0.78 0.74 0.66
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.3 0.1 0.19 0 0.06 0 0.1 0.07 0.09 0.11 0.1 0.12 0.12 0.06 0.12 0.64 0.71 0.64 0.31 0.24 0.25 0.17 0.2 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 1 5 5 1 0 4 6 2 3 1 3 1 3 0 0 1 0 1 0 0 0 0
Bud 1 1 1 2 2 6 4 3 2 2 2 4 0 1 1 0 0 0 0 1 0 2 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 3 2 9 7 9 11 10 8 10 8 12 2 8 9 1 1 2 0 0 0 0 0 0
Cytoplasm 116 108 52 65 117 131 174 238 199 188 153 178 24 37 36 35 32 33 15 8 3 2 2 2
Endoplasmic Reticulum 87 113 70 105 84 84 91 100 70 69 50 58 92 95 103 1 1 1 176 196 117 166 176 179
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1
Golgi 1 0 0 2 2 0 0 0 0 1 0 0 0 4 1 3 3 4 0 0 0 0 1 2
Mitochondria 0 0 0 1 0 2 3 0 1 2 0 2 0 0 0 0 0 0 1 0 0 0 1 0
Nucleus 1 0 0 0 0 0 0 1 0 0 0 1 0 1 0 3 2 6 0 0 0 0 0 1
Nuclear Periphery 0 0 1 2 0 1 1 0 1 0 1 0 0 0 0 0 1 0 7 2 5 2 2 5
Nucleolus 0 0 0 1 0 1 1 1 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 77 24 26 7 12 9 29 25 26 32 21 31 16 8 19 69 89 65 91 66 43 36 48 71
Unique Cell Count 253 231 140 176 212 228 293 362 295 286 219 267 128 140 155 107 125 102 296 280 171 214 239 271
Labelled Cell Count 286 250 153 199 229 244 314 382 313 306 238 288 137 155 172 112 129 113 296 280 171 214 239 271


Endoplasmic Reticulum, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 31.8 38.3 21.4 28.3 34.0 31.8 29.8 32.5 31.0 30.1 31.2 29.7 34.2 28.8 26.9 40.8 42.2 45.2 37.3 40.1 38.6
Std Deviation (1e-4) 7.0 9.7 5.7 7.9 9.8 8.7 8.5 7.9 9.2 8.4 9.8 8.7 7.7 8.0 7.2 12.2 12.3 13.3 10.3 12.7 12.1
Intensity Change (Log2) – – – 0.4 0.66 0.57 0.48 0.6 0.53 0.49 0.54 0.47 0.67 0.43 0.33 0.93 0.98 1.07 0.8 0.9 0.85


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1.9 2.0 0 0 0
Cytoplasm 0 3.3 3.8 4.3 5.8 6.0 5.6 6.1 5.7 -3.3 -1.9 -2.6 -0.7 -2.0 -0.8
Endoplasmic Reticulum 1.7 -1.9 -2.5 -3.8 -4.8 -5.5 -5.4 -5.3 -5.8 3.7 3.0 2.9 -8.4 -9.0 -8.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.2 -3.8 -4.6 -2.5 -3.9 -2.9 -2.1 -2.5 -1.9 -1.4 -3.3 -1.5 7.3 8.6 7.2