Standard name | |
---|---|
Human Ortholog | |
Description | Methylene-fatty-acyl-phospholipid synthase; catalyzes the last two steps in phosphatidylcholine biosynthesis; also known as phospholipid methyltransferase |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 1 | 5 | 5 | 1 | 0 | 4 | 6 | 2 | 3 | 1 | 3 | 1 | 3 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud | 1 | 1 | 1 | 2 | 2 | 6 | 4 | 3 | 2 | 2 | 2 | 4 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 3 | 2 | 9 | 7 | 9 | 11 | 10 | 8 | 10 | 8 | 12 | 2 | 8 | 9 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 116 | 108 | 52 | 65 | 117 | 131 | 174 | 238 | 199 | 188 | 153 | 178 | 24 | 37 | 36 | 35 | 32 | 33 | 15 | 8 | 3 | 2 | 2 | 2 |
Endoplasmic Reticulum | 87 | 113 | 70 | 105 | 84 | 84 | 91 | 100 | 70 | 69 | 50 | 58 | 92 | 95 | 103 | 1 | 1 | 1 | 176 | 196 | 117 | 166 | 176 | 179 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
Golgi | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 1 | 3 | 3 | 4 | 0 | 0 | 0 | 0 | 1 | 2 |
Mitochondria | 0 | 0 | 0 | 1 | 0 | 2 | 3 | 0 | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 2 | 6 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 7 | 2 | 5 | 2 | 2 | 5 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 77 | 24 | 26 | 7 | 12 | 9 | 29 | 25 | 26 | 32 | 21 | 31 | 16 | 8 | 19 | 69 | 89 | 65 | 91 | 66 | 43 | 36 | 48 | 71 |
Unique Cell Count | 253 | 231 | 140 | 176 | 212 | 228 | 293 | 362 | 295 | 286 | 219 | 267 | 128 | 140 | 155 | 107 | 125 | 102 | 296 | 280 | 171 | 214 | 239 | 271 |
Labelled Cell Count | 286 | 250 | 153 | 199 | 229 | 244 | 314 | 382 | 313 | 306 | 238 | 288 | 137 | 155 | 172 | 112 | 129 | 113 | 296 | 280 | 171 | 214 | 239 | 271 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 31.8 | 38.3 | 21.4 | 28.3 | 34.0 | 31.8 | 29.8 | 32.5 | 31.0 | 30.1 | 31.2 | 29.7 | 34.2 | 28.8 | 26.9 | 40.8 | 42.2 | 45.2 | 37.3 | 40.1 | 38.6 |
Std Deviation (1e-4) | 7.0 | 9.7 | 5.7 | 7.9 | 9.8 | 8.7 | 8.5 | 7.9 | 9.2 | 8.4 | 9.8 | 8.7 | 7.7 | 8.0 | 7.2 | 12.2 | 12.3 | 13.3 | 10.3 | 12.7 | 12.1 |
Intensity Change (Log2) | – | – | – | 0.4 | 0.66 | 0.57 | 0.48 | 0.6 | 0.53 | 0.49 | 0.54 | 0.47 | 0.67 | 0.43 | 0.33 | 0.93 | 0.98 | 1.07 | 0.8 | 0.9 | 0.85 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 2.0 | 0 | 0 | 0 |
Cytoplasm | 0 | 3.3 | 3.8 | 4.3 | 5.8 | 6.0 | 5.6 | 6.1 | 5.7 | -3.3 | -1.9 | -2.6 | -0.7 | -2.0 | -0.8 |
Endoplasmic Reticulum | 1.7 | -1.9 | -2.5 | -3.8 | -4.8 | -5.5 | -5.4 | -5.3 | -5.8 | 3.7 | 3.0 | 2.9 | -8.4 | -9.0 | -8.3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -4.2 | -3.8 | -4.6 | -2.5 | -3.9 | -2.9 | -2.1 | -2.5 | -1.9 | -1.4 | -3.3 | -1.5 | 7.3 | 8.6 | 7.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 32.2212 | 38.7672 | 33.5083 | 28.3789 | 32.486 | 35.2274 | 39.2674 | 50.1015 | 43.6663 | 40.7836 | 41.9466 | 45.3281 | 37.921 | 45.4359 | 38.0212 | 36.6986 | 34.1921 | 41.2838 |
Actin | 0.0082 | 0.0001 | 0.0109 | 0.0003 | 0.0059 | 0.0017 | 0.0078 | 0 | 0.0096 | 0.0001 | 0.0041 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 |
Bud | 0.0003 | 0.0001 | 0.0006 | 0.0006 | 0.0006 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 |
Bud Neck | 0.0007 | 0.0003 | 0.0007 | 0.001 | 0.001 | 0.0008 | 0.0003 | 0.0003 | 0.0006 | 0.0014 | 0.0007 | 0.0005 | 0.0003 | 0.0003 | 0.0008 | 0.0006 | 0.0005 | 0.0008 |
Bud Periphery | 0.0014 | 0.0008 | 0.0016 | 0.0025 | 0.0037 | 0.0018 | 0.001 | 0.0005 | 0.001 | 0.0023 | 0.0013 | 0.0011 | 0.0007 | 0.0006 | 0.0014 | 0.0021 | 0.0011 | 0.0017 |
Bud Site | 0.0014 | 0.0001 | 0.0025 | 0.0031 | 0.0006 | 0.0002 | 0.0002 | 0 | 0.0003 | 0.0002 | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 |
Cell Periphery | 0.1249 | 0.17 | 0.1071 | 0.1261 | 0.1037 | 0.108 | 0.0796 | 0.124 | 0.1267 | 0.1333 | 0.1127 | 0.0691 | 0.071 | 0.1284 | 0.1049 | 0.0817 | 0.0807 | 0.0651 |
Cytoplasm | 0.0093 | 0.0061 | 0.0143 | 0.0092 | 0.0074 | 0.0037 | 0.0098 | 0.0055 | 0.0092 | 0.0072 | 0.0099 | 0.0042 | 0.0063 | 0.0038 | 0.0062 | 0.0049 | 0.0041 | 0.0038 |
Cytoplasmic Foci | 0.003 | 0.0002 | 0.0092 | 0.0113 | 0.008 | 0.0015 | 0.0062 | 0 | 0.0024 | 0.0001 | 0.014 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Eisosomes | 0.0002 | 0 | 0.0005 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.255 | 0.211 | 0.2683 | 0.0677 | 0.0468 | 0.1333 | 0.3661 | 0.4339 | 0.2922 | 0.2009 | 0.1659 | 0.2651 | 0.4014 | 0.4361 | 0.407 | 0.3058 | 0.2586 | 0.4507 |
Endosome | 0.0076 | 0.0016 | 0.0135 | 0.0094 | 0.0056 | 0.0037 | 0.0082 | 0.0009 | 0.0043 | 0.0027 | 0.0097 | 0.002 | 0.0027 | 0.0006 | 0.0023 | 0.0103 | 0.0029 | 0.0032 |
Golgi | 0.0017 | 0.0001 | 0.0037 | 0.0023 | 0.003 | 0.0004 | 0.0007 | 0 | 0.0006 | 0.0001 | 0.0017 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0002 |
Lipid Particles | 0.007 | 0.0045 | 0.0101 | 0.0081 | 0.0255 | 0.0057 | 0.0083 | 0.0033 | 0.0117 | 0.0035 | 0.0083 | 0.0029 | 0.0066 | 0.0039 | 0.0033 | 0.0021 | 0.0058 | 0.0081 |
Mitochondria | 0.0082 | 0.0001 | 0.002 | 0.0103 | 0.0018 | 0.002 | 0.0019 | 0.0001 | 0.0012 | 0.0003 | 0.0004 | 0.0001 | 0.0008 | 0 | 0.0002 | 0.0007 | 0.0002 | 0.0003 |
None | 0.0006 | 0 | 0.0079 | 0.0042 | 0.0005 | 0.0011 | 0.0045 | 0 | 0.003 | 0.0001 | 0.0024 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Nuclear Periphery | 0.0957 | 0.0458 | 0.0461 | 0.0303 | 0.0408 | 0.0713 | 0.13 | 0.0543 | 0.0626 | 0.0884 | 0.1067 | 0.1121 | 0.2201 | 0.0767 | 0.0787 | 0.0793 | 0.087 | 0.1252 |
Nucleolus | 0.0034 | 0.0017 | 0.0038 | 0.0082 | 0.0013 | 0.0057 | 0.0091 | 0.0002 | 0.0026 | 0.0009 | 0.002 | 0.0004 | 0.0052 | 0.0001 | 0.0004 | 0.0049 | 0.0012 | 0.0006 |
Nucleus | 0.0096 | 0.0036 | 0.0079 | 0.0094 | 0.0061 | 0.009 | 0.0119 | 0.0032 | 0.0045 | 0.0065 | 0.0142 | 0.0037 | 0.0122 | 0.0026 | 0.0032 | 0.0095 | 0.0046 | 0.0077 |
Peroxisomes | 0.0005 | 0 | 0.0039 | 0.0011 | 0.046 | 0.0001 | 0.0004 | 0 | 0.0011 | 0 | 0.0031 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0006 | 0.0001 | 0.0094 | 0.0006 | 0.0046 | 0.001 | 0.0035 | 0 | 0.0008 | 0 | 0.0062 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 |
Vacuole | 0.4168 | 0.5292 | 0.4449 | 0.6471 | 0.6192 | 0.592 | 0.3073 | 0.3579 | 0.4263 | 0.5015 | 0.4875 | 0.4777 | 0.2421 | 0.3269 | 0.3637 | 0.4336 | 0.4637 | 0.2975 |
Vacuole Periphery | 0.0442 | 0.0247 | 0.031 | 0.0467 | 0.068 | 0.0567 | 0.0428 | 0.0159 | 0.039 | 0.0503 | 0.0487 | 0.061 | 0.0296 | 0.0197 | 0.0274 | 0.0634 | 0.0891 | 0.0347 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 163.4673 | 127.0694 | 90.7551 | 85.4827 | 85.2378 | 159.8252 | 111.6168 | 103.5162 | 139.4716 | 106.8394 |
Translational Efficiency | 1.2524 | 1.1493 | 0.9656 | 1.3972 | 0.9821 | 1.5155 | 0.991 | 1.0841 | 0.9215 | 0.8619 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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