Standard name | |
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Description | Methylene-fatty-acyl-phospholipid synthase; catalyzes the last two steps in phosphatidylcholine biosynthesis; also known as phospholipid methyltransferase |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 1 | 5 | 5 | 1 | 0 | 4 | 6 | 2 | 3 | 1 | 3 | 1 | 3 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud | 1 | 1 | 1 | 2 | 2 | 6 | 4 | 3 | 2 | 2 | 2 | 4 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 3 | 2 | 9 | 7 | 9 | 11 | 10 | 8 | 10 | 8 | 12 | 2 | 8 | 9 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 116 | 108 | 52 | 65 | 117 | 131 | 174 | 238 | 199 | 188 | 153 | 178 | 24 | 37 | 36 | 35 | 32 | 33 | 15 | 8 | 3 | 2 | 2 | 2 |
Endoplasmic Reticulum | 87 | 113 | 70 | 105 | 84 | 84 | 91 | 100 | 70 | 69 | 50 | 58 | 92 | 95 | 103 | 1 | 1 | 1 | 176 | 196 | 117 | 166 | 176 | 179 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
Golgi | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 1 | 3 | 3 | 4 | 0 | 0 | 0 | 0 | 1 | 2 |
Mitochondria | 0 | 0 | 0 | 1 | 0 | 2 | 3 | 0 | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 2 | 6 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 7 | 2 | 5 | 2 | 2 | 5 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 77 | 24 | 26 | 7 | 12 | 9 | 29 | 25 | 26 | 32 | 21 | 31 | 16 | 8 | 19 | 69 | 89 | 65 | 91 | 66 | 43 | 36 | 48 | 71 |
Unique Cell Count | 253 | 231 | 140 | 176 | 212 | 228 | 293 | 362 | 295 | 286 | 219 | 267 | 128 | 140 | 155 | 107 | 125 | 102 | 296 | 280 | 171 | 214 | 239 | 271 |
Labelled Cell Count | 286 | 250 | 153 | 199 | 229 | 244 | 314 | 382 | 313 | 306 | 238 | 288 | 137 | 155 | 172 | 112 | 129 | 113 | 296 | 280 | 171 | 214 | 239 | 271 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 31.8 | 38.3 | 21.4 | 28.3 | 34.0 | 31.8 | 29.8 | 32.5 | 31.0 | 30.1 | 31.2 | 29.7 | 34.2 | 28.8 | 26.9 | 40.8 | 42.2 | 45.2 | 37.3 | 40.1 | 38.6 |
Std Deviation (1e-4) | 7.0 | 9.7 | 5.7 | 7.9 | 9.8 | 8.7 | 8.5 | 7.9 | 9.2 | 8.4 | 9.8 | 8.7 | 7.7 | 8.0 | 7.2 | 12.2 | 12.3 | 13.3 | 10.3 | 12.7 | 12.1 |
Intensity Change (Log2) | – | – | – | 0.4 | 0.66 | 0.57 | 0.48 | 0.6 | 0.53 | 0.49 | 0.54 | 0.47 | 0.67 | 0.43 | 0.33 | 0.93 | 0.98 | 1.07 | 0.8 | 0.9 | 0.85 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 2.0 | 0 | 0 | 0 |
Cytoplasm | 0 | 3.3 | 3.8 | 4.3 | 5.8 | 6.0 | 5.6 | 6.1 | 5.7 | -3.3 | -1.9 | -2.6 | -0.7 | -2.0 | -0.8 |
Endoplasmic Reticulum | 1.7 | -1.9 | -2.5 | -3.8 | -4.8 | -5.5 | -5.4 | -5.3 | -5.8 | 3.7 | 3.0 | 2.9 | -8.4 | -9.0 | -8.3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -4.2 | -3.8 | -4.6 | -2.5 | -3.9 | -2.9 | -2.1 | -2.5 | -1.9 | -1.4 | -3.3 | -1.5 | 7.3 | 8.6 | 7.2 |