Standard name | |
---|---|
Human Ortholog | |
Description | Ribosomal 60S subunit protein L41A; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41A has a paralog, RPL41B, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | – | 3 | 4 | 8 | 11 | 16 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 2 | 1 | 1 | 1 | – | 1 | 2 | 0 | 4 | 2 | 3 | 4 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 7 | 14 | – | 12 | 14 | 21 | 19 | 20 | 2 | 3 | 6 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 316 | 137 | 130 | 217 | 284 | 307 | – | 364 | 276 | 270 | 245 | 272 | 472 | 806 | 825 | 218 | 353 | 336 | 303 | 129 | 117 | 114 | 403 | 574 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 2 | 20 | – | 90 | 44 | 54 | 42 | 52 | 2 | 5 | 9 | 13 | 18 | 26 | 0 | 1 | 0 | 0 | 1 | 2 |
Endosome | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 3 | 5 | 5 | 9 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 5 | 3 | 11 | 21 | 0 | 0 | 0 | 0 | 2 | 6 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 3 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 2 | 2 | 2 | 4 | 7 | 5 |
Unique Cell Count | 318 | 138 | 130 | 217 | 290 | 333 | 466 | 334 | 345 | 314 | 368 | 475 | 814 | 834 | 221 | 356 | 342 | 311 | 141 | 122 | 125 | 425 | 601 | |
Labelled Cell Count | 318 | 138 | 132 | 219 | 296 | 343 | 474 | 348 | 362 | 337 | 395 | 481 | 819 | 844 | 237 | 376 | 372 | 311 | 141 | 122 | 125 | 425 | 601 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 196.0 | 241.8 | 108.9 | 156.9 | 128.2 | 98.6 | – | 73.4 | 64.5 | 59.2 | 54.3 | 49.8 | 205.8 | 207.6 | 186.2 | 262.7 | 240.1 | 250.3 | 187.4 | 211.2 | 225.8 |
Std Deviation (1e-4) | 42.1 | 52.4 | 25.5 | 32.3 | 32.1 | 22.7 | – | 17.9 | 15.9 | 13.0 | 12.7 | 12.5 | 50.7 | 46.1 | 43.1 | 86.9 | 91.2 | 82.2 | 47.1 | 59.3 | 63.9 |
Intensity Change (Log2) | – | – | – | 0.53 | 0.24 | -0.14 | – | -0.57 | -0.75 | -0.88 | -1.0 | -1.13 | 0.92 | 0.93 | 0.77 | 1.27 | 1.14 | 1.2 | 0.78 | 0.96 | 1.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 2.9 | 2.9 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | -1.7 | -3.3 | – | -5.9 | -5.1 | -5.8 | -5.8 | -6.5 | -0.9 | -1.1 | -1.2 | -1.3 | -1.0 | -1.5 |
Endoplasmic Reticulum | 0 | 0 | 2.9 | – | 5.4 | 4.3 | 4.8 | 4.4 | 4.5 | 0 | 0 | 0 | 0 | 0 | 3.2 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 160.8752 | 285.6369 | 222.2298 | 172.1388 | 211.4679 | 233.9378 | 192.9565 | 277.8344 | 233.928 | 212.5582 | 229.0459 | 190.0723 | 210.1999 | 285.7337 | 289.8093 | 257.5035 | 280.1833 | 211.3374 |
Actin | 0.0103 | 0.0004 | 0.002 | 0.0014 | 0.0054 | 0.0077 | 0.022 | 0.0003 | 0.0018 | 0.0077 | 0.0017 | 0.0021 | 0.0301 | 0.0023 | 0.0008 | 0.0013 | 0.0006 | 0.0034 |
Bud | 0.0076 | 0.001 | 0.0025 | 0.0041 | 0.0077 | 0.0048 | 0.0037 | 0.0007 | 0.0036 | 0.003 | 0.0026 | 0.0011 | 0.0042 | 0.002 | 0.0033 | 0.007 | 0.0014 | 0.0026 |
Bud Neck | 0.0027 | 0.0009 | 0.0014 | 0.0014 | 0.002 | 0.0045 | 0.0016 | 0.0009 | 0.0013 | 0.0018 | 0.0014 | 0.0032 | 0.0014 | 0.0006 | 0.0014 | 0.0014 | 0.0006 | 0.0011 |
Bud Periphery | 0.0078 | 0.0006 | 0.0019 | 0.0052 | 0.0063 | 0.0069 | 0.0057 | 0.0004 | 0.0043 | 0.0013 | 0.0013 | 0.0019 | 0.0033 | 0.0007 | 0.0056 | 0.0051 | 0.0002 | 0.0007 |
Bud Site | 0.0047 | 0.0013 | 0.0023 | 0.0023 | 0.0055 | 0.0012 | 0.0045 | 0.0011 | 0.0025 | 0.0015 | 0.0008 | 0.0009 | 0.0083 | 0.0108 | 0.0009 | 0.0016 | 0.0001 | 0.0004 |
Cell Periphery | 0.0735 | 0.0574 | 0.0545 | 0.0537 | 0.0332 | 0.02 | 0.0806 | 0.0469 | 0.0347 | 0.0558 | 0.0107 | 0.0411 | 0.0291 | 0.0321 | 0.0197 | 0.0352 | 0.0016 | 0.0029 |
Cytoplasm | 0.4047 | 0.7947 | 0.5962 | 0.3124 | 0.5705 | 0.6266 | 0.4856 | 0.8529 | 0.7733 | 0.3928 | 0.7181 | 0.5178 | 0.5453 | 0.8545 | 0.8254 | 0.5401 | 0.9115 | 0.5954 |
Cytoplasmic Foci | 0.0291 | 0.0005 | 0.0224 | 0.0364 | 0.021 | 0.0117 | 0.0141 | 0.0005 | 0.0026 | 0.0034 | 0.0205 | 0.0065 | 0.0174 | 0.0021 | 0.0012 | 0.0048 | 0.0015 | 0.0415 |
Eisosomes | 0.0017 | 0 | 0.0007 | 0.0022 | 0.0025 | 0.0001 | 0.0012 | 0.0001 | 0.001 | 0 | 0.0012 | 0.0004 | 0.0027 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0005 |
Endoplasmic Reticulum | 0.0316 | 0.0041 | 0.0097 | 0.0057 | 0.0038 | 0.0175 | 0.0171 | 0.0028 | 0.0024 | 0.0027 | 0.0054 | 0.042 | 0.0169 | 0.0012 | 0.0018 | 0.0016 | 0.0003 | 0.004 |
Endosome | 0.0185 | 0.0007 | 0.0097 | 0.0557 | 0.0127 | 0.0306 | 0.008 | 0.0004 | 0.0016 | 0.004 | 0.0056 | 0.0053 | 0.0125 | 0.0007 | 0.0014 | 0.0026 | 0.0008 | 0.0433 |
Golgi | 0.0029 | 0.0001 | 0.0028 | 0.0038 | 0.0019 | 0.0053 | 0.0026 | 0.0001 | 0.0003 | 0.0004 | 0.0009 | 0.001 | 0.0034 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0044 |
Lipid Particles | 0.0102 | 0.0014 | 0.0054 | 0.0117 | 0.013 | 0.0085 | 0.0068 | 0.0011 | 0.0035 | 0.0038 | 0.0057 | 0.004 | 0.0081 | 0.0012 | 0.0007 | 0.0016 | 0.0003 | 0.0352 |
Mitochondria | 0.0248 | 0.0009 | 0.0172 | 0.0203 | 0.0198 | 0.0149 | 0.0207 | 0.0009 | 0.0115 | 0.0011 | 0.0126 | 0.0168 | 0.0291 | 0.0029 | 0.0128 | 0.0193 | 0.0021 | 0.0161 |
None | 0.0219 | 0.0006 | 0.013 | 0.0281 | 0.0081 | 0.0018 | 0.0109 | 0.001 | 0.0039 | 0.0007 | 0.0132 | 0.0284 | 0.0199 | 0.0015 | 0.0014 | 0.0181 | 0.0015 | 0.0113 |
Nuclear Periphery | 0.0677 | 0.0102 | 0.0197 | 0.0224 | 0.0158 | 0.0339 | 0.1039 | 0.0101 | 0.0161 | 0.0408 | 0.0288 | 0.0743 | 0.064 | 0.0069 | 0.0109 | 0.016 | 0.0141 | 0.0598 |
Nucleolus | 0.0321 | 0.002 | 0.0167 | 0.0382 | 0.0116 | 0.0031 | 0.0094 | 0.0025 | 0.0212 | 0.0068 | 0.0253 | 0.0463 | 0.0189 | 0.0029 | 0.0019 | 0.0116 | 0.0054 | 0.0891 |
Nucleus | 0.0357 | 0.0102 | 0.0203 | 0.0223 | 0.0209 | 0.0307 | 0.0529 | 0.0125 | 0.0225 | 0.0281 | 0.0442 | 0.0967 | 0.0656 | 0.0122 | 0.0136 | 0.0393 | 0.0295 | 0.0458 |
Peroxisomes | 0.0014 | 0.0001 | 0.0011 | 0.0015 | 0.0035 | 0.0037 | 0.0011 | 0.0001 | 0.0005 | 0.0003 | 0.001 | 0.0003 | 0.0014 | 0.0002 | 0.0002 | 0.0006 | 0.0001 | 0.002 |
Punctate Nuclear | 0.0019 | 0.0001 | 0.0011 | 0.0013 | 0.0009 | 0.0036 | 0.0073 | 0.0001 | 0.0004 | 0.0004 | 0.0025 | 0.0044 | 0.0031 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0109 |
Vacuole | 0.1734 | 0.1047 | 0.1649 | 0.2587 | 0.1848 | 0.1094 | 0.1116 | 0.0596 | 0.0738 | 0.3227 | 0.0673 | 0.0768 | 0.0842 | 0.0572 | 0.0793 | 0.2096 | 0.0137 | 0.0152 |
Vacuole Periphery | 0.0358 | 0.0081 | 0.0345 | 0.1114 | 0.0488 | 0.0534 | 0.0287 | 0.0049 | 0.0173 | 0.1208 | 0.0294 | 0.0285 | 0.0312 | 0.0076 | 0.0173 | 0.0821 | 0.0142 | 0.0144 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8275.5333 | 10205.1996 | 14995.2567 | 7777.3471 | 14333.6327 | 14619.0843 | 8934.3602 | 10247.9185 | 14689.8232 | 7075.598 |
Translational Efficiency | 2.5456 | 2.2162 | 1.3227 | 3.7872 | 1.581 | 1.2385 | 1.9004 | 2.0393 | 1.2259 | 2.1895 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
29 | 1926 | 329 | 887 | 781 | 1092 | 2137 | 1425 | 810 | 3018 | 2466 | 2312 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 6691.54 | 13760.87 | 14653.79 | 15079.09 | 11216.23 | 13103.74 | 15713.68 | 12280.89 | 11054.23 | 13523.10 | 15572.28 | 13354.42 |
Standard Deviation | 2934.82 | 3500.45 | 2342.30 | 3120.82 | 2596.32 | 3363.13 | 2868.91 | 3605.40 | 2741.28 | 3465.81 | 2827.44 | 3687.81 |
Intensity Change Log 2 | — | 1.040162 | 1.130864 | 1.172139 | — | 0.224391 | 0.486433 | 0.130827 | — | 0.585121 | 0.761941 | 0.611481 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001053 | 0.000049 | 0.000138 | 0.000134 | 0.000068 | 0.000069 | 0.000064 | 0.000160 | 0.000103 | 0.000056 | 0.000073 | 0.000150 |
Bud Neck | 0.001931 | 0.000294 | 0.004815 | 0.001540 | 0.000654 | 0.000890 | 0.000440 | 0.001599 | 0.000700 | 0.000510 | 0.001024 | 0.001577 |
Bud Site | 0.002640 | 0.002896 | 0.003536 | 0.004901 | 0.001495 | 0.002395 | 0.001897 | 0.005882 | 0.001536 | 0.002714 | 0.002115 | 0.005505 |
Cell Periphery | 0.080332 | 0.010866 | 0.045299 | 0.032101 | 0.047252 | 0.011608 | 0.023415 | 0.018401 | 0.048437 | 0.011135 | 0.026334 | 0.023657 |
Cytoplasm | 0.078923 | 0.692943* | 0.244305* | 0.479196* | 0.367014* | 0.625726* | 0.644807* | 0.656066* | 0.356699* | 0.668622* | 0.591374* | 0.588210* |
Cytoplasmic Foci | 0.011016 | 0.015916 | 0.018484 | 0.006439 | 0.011189 | 0.016191 | 0.004159 | 0.006186 | 0.011183 | 0.016016 | 0.006070 | 0.006283 |
Eisosomes | 0.000031 | 0.000001 | 0.000008 | 0.000004 | 0.000004 | 0.000002 | 0.000002 | 0.000004 | 0.000005 | 0.000002 | 0.000002 | 0.000004 |
Endoplasmic Reticulum | 0.004084 | 0.000947 | 0.000352 | 0.000246 | 0.001308 | 0.000359 | 0.000637 | 0.000179 | 0.001408 | 0.000735 | 0.000599 | 0.000205 |
Endosome | 0.001922 | 0.000677 | 0.000889 | 0.000732 | 0.000597 | 0.000691 | 0.000328 | 0.000774 | 0.000644 | 0.000682 | 0.000403 | 0.000758 |
Golgi | 0.178833* | 0.066778 | 0.069664 | 0.021276 | 0.114461* | 0.075633 | 0.025812 | 0.014900 | 0.116766* | 0.069982 | 0.031662 | 0.017346 |
Lipid Particles | 0.031338 | 0.007297 | 0.025709 | 0.017933 | 0.024224 | 0.008256 | 0.012844 | 0.012029 | 0.024478 | 0.007644 | 0.014560 | 0.014294 |
Mitochondria | 0.003609 | 0.000546 | 0.000202 | 0.000343 | 0.000200 | 0.000323 | 0.000195 | 0.000373 | 0.000322 | 0.000466 | 0.000196 | 0.000362 |
Mitotic Spindle | 0.001577 | 0.001075 | 0.002508 | 0.000666 | 0.001106 | 0.002872 | 0.000412 | 0.002323 | 0.001123 | 0.001725 | 0.000692 | 0.001687 |
None | 0.004881 | 0.002864 | 0.001272 | 0.001316 | 0.001948 | 0.003554 | 0.001162 | 0.005699 | 0.002053 | 0.003114 | 0.001177 | 0.004018 |
Nuclear Periphery | 0.004489 | 0.000750 | 0.005092 | 0.000845 | 0.002183 | 0.001033 | 0.000825 | 0.000432 | 0.002266 | 0.000852 | 0.001394 | 0.000590 |
Nuclear Periphery Foci | 0.030206 | 0.009373 | 0.020093 | 0.007315 | 0.019792 | 0.014327 | 0.007555 | 0.005277 | 0.020165 | 0.011165 | 0.009228 | 0.006059 |
Nucleolus | 0.003665 | 0.000065 | 0.000194 | 0.000240 | 0.000107 | 0.000164 | 0.000096 | 0.000376 | 0.000234 | 0.000101 | 0.000109 | 0.000324 |
Nucleus | 0.057288 | 0.005538 | 0.019208 | 0.018568 | 0.006459 | 0.013596 | 0.006892 | 0.036049 | 0.008278 | 0.008454 | 0.008535 | 0.029342 |
Peroxisomes | 0.000504 | 0.000019 | 0.000017 | 0.000048 | 0.000011 | 0.000015 | 0.000037 | 0.000152 | 0.000029 | 0.000018 | 0.000035 | 0.000113 |
Vacuole | 0.240691* | 0.061712 | 0.382214* | 0.340479* | 0.177518 | 0.104826 | 0.196093 | 0.200334* | 0.179780 | 0.077312 | 0.220924* | 0.254101* |
Vacuole Periphery | 0.260987* | 0.119393* | 0.156002* | 0.065677 | 0.222409* | 0.117469* | 0.072329 | 0.032804 | 0.223790* | 0.118697* | 0.083492 | 0.045416 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.80 | 1.59 | 1.63 | -3.01 | 0.53 | -0.38 | 0.15 | -4.07 | -3.74 | -4.23 | 2.02 | 1.03 | -2.26 | -5.20 | -4.19 |
Bud Neck | 2.07 | -3.87 | -0.48 | -8.88 | 4.71 | -1.26 | 1.68 | -8.36 | -5.73 | -9.39 | 1.32 | -3.66 | -9.81 | -10.76 | -6.66 |
Bud Site | -0.10 | -0.82 | -1.72 | -3.06 | -1.55 | -1.90 | -0.78 | -6.71 | -5.52 | -6.69 | -2.61 | -1.43 | -7.68 | -6.03 | -7.49 |
Cell Periphery | 3.35 | 1.74 | 2.48 | -8.16 | 4.00 | 12.73 | 8.32 | 11.20 | -2.34 | 5.63 | 13.61 | 7.81 | 9.87 | -7.55 | 4.19 |
Cytoplasm | -18.13 | -4.33 | -11.07 | 16.11 | -11.68 | -15.28 | -18.33 | -17.12 | -0.46 | 0.71 | -22.26 | -15.76 | -14.69 | 10.58 | 1.65 |
Cytoplasmic Foci | -1.17 | -2.47 | 0.41 | 3.67 | 4.55 | -3.71 | 6.85 | 1.82 | 5.28 | -4.43 | -4.15 | 4.44 | 1.73 | 6.52 | -2.78 |
Eisosomes | 2.20 | 1.58 | 1.97 | -4.20 | 4.16 | 2.92 | 4.26 | -0.54 | -2.65 | -3.40 | 4.49 | 2.82 | 0.52 | -4.85 | -2.74 |
Endoplasmic Reticulum | 1.31 | 1.57 | 1.62 | 6.34 | 1.73 | 3.65 | 2.56 | 4.43 | 4.44 | 6.65 | 2.46 | 3.04 | 4.64 | 7.42 | 6.59 |
Endosome | 1.50 | 1.19 | 1.41 | -0.11 | 0.81 | -1.47 | 5.99 | -0.77 | -0.27 | -2.08 | -0.66 | 4.31 | -0.57 | -0.32 | -2.07 |
Golgi | 3.14 | 3.21 | 4.56 | 17.79 | 12.03 | 7.68 | 21.91 | 25.40 | 19.04 | 11.64 | 10.41 | 20.79 | 25.03 | 26.83 | 14.10 |
Lipid Particles | 3.05 | 0.73 | 1.84 | -6.87 | 4.00 | 9.01 | 6.33 | 6.97 | -2.33 | 1.77 | 10.10 | 5.62 | 6.26 | -6.39 | 1.48 |
Mitochondria | 1.29 | 1.41 | 1.36 | 1.36 | -1.17 | -1.20 | 0.13 | -2.73 | -0.42 | -3.01 | -1.19 | 1.30 | -0.53 | 0.91 | -3.16 |
Mitotic Spindle | 0.52 | -1.10 | 0.91 | 2.72 | 3.80 | -2.29 | 6.58 | -2.62 | -0.84 | -3.29 | -1.96 | 3.43 | -2.34 | -1.39 | -3.01 |
None | 0.67 | 1.14 | 1.13 | 2.78 | -0.13 | -1.83 | 3.13 | -3.17 | -1.14 | -3.90 | -2.18 | 3.48 | -2.46 | -0.63 | -3.82 |
Nuclear Periphery | 1.80 | -0.48 | 1.68 | -2.33 | 9.27 | 8.50 | 10.33 | 11.38 | 4.38 | 2.73 | 9.99 | 5.04 | 10.53 | 1.48 | 7.11 |
Nuclear Periphery Foci | 1.05 | 0.50 | 1.14 | 2.02 | 6.56 | 3.57 | 10.50 | 12.31 | 8.60 | 4.49 | 6.55 | 8.22 | 10.65 | 8.15 | 5.93 |
Nucleolus | 1.68 | 1.60 | 1.55 | -6.25 | -2.34 | -1.80 | 0.75 | -3.06 | -2.67 | -3.12 | 1.63 | 1.51 | -2.18 | -3.81 | -3.73 |
Nucleus | 1.76 | 1.14 | 1.09 | -9.25 | -0.47 | -4.03 | -0.57 | -14.59 | -11.95 | -15.23 | -0.08 | -0.64 | -13.28 | -16.16 | -15.67 |
Peroxisomes | 1.04 | 1.04 | 0.96 | -2.58 | -2.65 | -0.91 | -6.39 | -1.95 | -1.88 | -1.54 | 0.66 | -0.30 | -1.74 | -2.19 | -1.76 |
Vacuole | 3.96 | -3.09 | -2.28 | -27.94 | 2.41 | 8.18 | -2.79 | -3.25 | -12.19 | -0.87 | 13.77 | -5.49 | -9.14 | -29.29 | -4.73 |
Vacuole Periphery | 3.16 | 2.57 | 4.61 | 12.24 | 10.38 | 11.40 | 18.76 | 24.91 | 16.10 | 15.62* | 12.88 | 17.99 | 23.91 | 22.62 | 14.98 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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