Standard name
Description Ribosomal 60S subunit protein L41A; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41A has a paralog, RPL41B, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0.06 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 1.0 1.0 0.98 0.92 – 0.78 0.83 0.78 0.78 0.74 0.99 0.99 0.99 0.99 0.99 0.98 0.98 0.92 0.96 0.92 0.95 0.96
Endoplasmic Reticulum 0 0 0 0 0 0.06 – 0.19 0.13 0.16 0.13 0.14 0 0 0 0.06 0.05 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 – 3 4 8 11 16 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 2 1 1 1 – 1 2 0 4 2 3 4 3 0 0 0 0 1 0 0 3 8
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 7 14 – 12 14 21 19 20 2 3 6 3 0 1 0 0 0 0 0 0
Cytoplasm 316 137 130 217 284 307 – 364 276 270 245 272 472 806 825 218 353 336 303 129 117 114 403 574
Endoplasmic Reticulum 0 0 0 0 2 20 – 90 44 54 42 52 2 5 9 13 18 26 0 1 0 0 1 2
Endosome 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 1 0 – 1 3 5 5 9 1 0 0 0 1 2 0 0 0 0 0 0
Mitochondria 0 0 0 0 1 0 – 1 5 3 11 21 0 0 0 0 2 6 0 0 1 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 1 0 1 1 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 1 0 0 0 3 1 0 1 2 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 0 0 0 – 1 0 0 0 0 0 1 0 1 2 1 2 2 2 4 7 5
Unique Cell Count 318 138 130 217 290 333 466 334 345 314 368 475 814 834 221 356 342 311 141 122 125 425 601
Labelled Cell Count 318 138 132 219 296 343 474 348 362 337 395 481 819 844 237 376 372 311 141 122 125 425 601


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 196.0 241.8 108.9 156.9 128.2 98.6 – 73.4 64.5 59.2 54.3 49.8 205.8 207.6 186.2 262.7 240.1 250.3 187.4 211.2 225.8
Std Deviation (1e-4) 42.1 52.4 25.5 32.3 32.1 22.7 – 17.9 15.9 13.0 12.7 12.5 50.7 46.1 43.1 86.9 91.2 82.2 47.1 59.3 63.9
Intensity Change (Log2) – – – 0.53 0.24 -0.14 – -0.57 -0.75 -0.88 -1.0 -1.13 0.92 0.93 0.77 1.27 1.14 1.2 0.78 0.96 1.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 2.9 2.9 2.7 0 0 0 0 0 0
Cytoplasm 0 -1.7 -3.3 – -5.9 -5.1 -5.8 -5.8 -6.5 -0.9 -1.1 -1.2 -1.3 -1.0 -1.5
Endoplasmic Reticulum 0 0 2.9 – 5.4 4.3 4.8 4.4 4.5 0 0 0 0 0 3.2
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 2.8 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0