ORF | |
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Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 2 |
Bud | 0 | 1 | 1 | 3 | 1 | 7 | 18 | 11 | 18 | 22 | 26 | 19 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 214 | 251 | 403 | 339 | 384 | 628 | 775 | 788 | 508 | 519 | 588 | 735 | 154 | 195 | 195 | 165 | 131 | 152 | 88 | 150 | 114 | 48 | 104 | 144 |
Endoplasmic Reticulum | 1 | 2 | 3 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 6 | 5 | 10 | 0 | 0 | 7 | 1 | 5 | 6 |
Endosome | 0 | 1 | 1 | 2 | 7 | 2 | 4 | 5 | 0 | 3 | 3 | 17 | 0 | 0 | 0 | 1 | 2 | 2 | 3 | 2 | 11 | 0 | 3 | 4 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 0 |
Mitochondria | 4 | 15 | 7 | 9 | 10 | 101 | 177 | 157 | 171 | 168 | 232 | 211 | 0 | 0 | 0 | 4 | 7 | 3 | 3 | 4 | 18 | 11 | 19 | 12 |
Nucleus | 0 | 0 | 0 | 2 | 1 | 0 | 5 | 13 | 2 | 4 | 6 | 2 | 1 | 0 | 1 | 10 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 6 | 5 | 8 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 1 | 0 | 0 | 1 | 0 | 0 | 5 | 1 | 2 | 1 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 2 | 3 | 3 | 9 | 0 | 4 | 9 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 3 |
Vac/Vac Membrane | 16 | 1 | 23 | 9 | 67 | 44 | 34 | 32 | 35 | 43 | 41 | 76 | 0 | 0 | 0 | 62 | 43 | 51 | 153 | 112 | 226 | 61 | 114 | 150 |
Unique Cell Count | 226 | 263 | 418 | 353 | 423 | 694 | 885 | 905 | 594 | 624 | 731 | 917 | 156 | 197 | 196 | 221 | 166 | 203 | 257 | 279 | 411 | 128 | 259 | 339 |
Labelled Cell Count | 236 | 272 | 440 | 365 | 474 | 792 | 1027 | 1026 | 738 | 766 | 911 | 1074 | 157 | 198 | 198 | 250 | 191 | 225 | 257 | 279 | 411 | 128 | 259 | 339 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 3.5 | 4.2 | 4.0 | 4.1 | 3.3 | 3.5 | 3.7 | 3.3 | 3.3 | 3.3 | 3.3 | 4.7 | 4.6 | 4.7 | 5.3 | 5.2 | 5.0 | 4.4 | 4.6 | 4.8 |
Std Deviation (1e-4) | 0.6 | 0.9 | 2.2 | 2.0 | 1.6 | 1.4 | 1.8 | 1.3 | 1.3 | 1.2 | 1.1 | 0.9 | 1.1 | 1.1 | 0.8 | 1.0 | 1.0 | 1.0 | 1.2 | 1.3 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.02 | -0.35 | -0.27 | -0.19 | -0.36 | -0.32 | -0.36 | -0.33 | 0.18 | 0.15 | 0.16 | 0.33 | 0.31 | 0.26 | 0.07 | 0.15 | 0.19 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 8.0 | 7.0 | 7.1 |
R1 | R2 | R3 | ||||||||||||||||
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G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.5241 | -0.9063 | -1.0444 | -1.147 | -1.078 | -0.9718 | 0.0873 | 0.8072 | 0.5829 | 0.6357 | 0.4572 | 0.7114 | -1.0151 | -0.2559 | -0.6135 | -0.4699 | 0.3574 | -0.4379 |
Actin | 0.0037 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0195 | 0.0001 | 0.0022 | 0.0076 | 0.0137 | 0.0041 | 0.001 | 0 | 0.0125 | 0.0002 | 0.0015 | 0.0005 |
Bud | 0.0002 | 0.0002 | 0.0007 | 0.0064 | 0.0004 | 0.0003 | 0.0011 | 0.0033 | 0.0015 | 0.0029 | 0.0038 | 0.0009 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0007 |
Bud Neck | 0.0004 | 0.0051 | 0.0003 | 0.0008 | 0.0003 | 0.0032 | 0.0039 | 0.0015 | 0.0009 | 0.0007 | 0.0012 | 0.005 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0008 | 0.0022 |
Bud Periphery | 0.0003 | 0.0004 | 0.0007 | 0.0104 | 0.0007 | 0.0005 | 0.0023 | 0.0122 | 0.0028 | 0.0059 | 0.0043 | 0.0019 | 0.0001 | 0.0003 | 0.0005 | 0.0002 | 0.0005 | 0.0007 |
Bud Site | 0.0016 | 0.0033 | 0.002 | 0.0084 | 0.0003 | 0.0004 | 0.0044 | 0.0171 | 0.004 | 0.0008 | 0.0016 | 0.0007 | 0.0002 | 0.0006 | 0.0024 | 0.0003 | 0.0011 | 0.0075 |
Cell Periphery | 0.0002 | 0.0005 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0009 | 0.0013 | 0.0004 | 0.0004 | 0.0005 | 0.0005 | 0.0002 | 0.0006 | 0.0002 | 0.0001 | 0.0017 | 0.0007 |
Cytoplasm | 0.1005 | 0.1168 | 0.0906 | 0.1493 | 0.1883 | 0.217 | 0.1269 | 0.2125 | 0.1826 | 0.1184 | 0.1643 | 0.2397 | 0.1422 | 0.1594 | 0.1706 | 0.0864 | 0.1714 | 0.2226 |
Cytoplasmic Foci | 0.0192 | 0.0306 | 0.0341 | 0.0844 | 0.0175 | 0.0441 | 0.0588 | 0.0623 | 0.0547 | 0.0907 | 0.0721 | 0.0608 | 0.0319 | 0.0234 | 0.0417 | 0.0177 | 0.0181 | 0.0513 |
Eisosomes | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0002 | 0.0055 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0013 | 0.0001 |
Endoplasmic Reticulum | 0.0023 | 0.0016 | 0.0022 | 0.0021 | 0.0033 | 0.003 | 0.0106 | 0.0194 | 0.0021 | 0.0022 | 0.0023 | 0.0026 | 0.0022 | 0.0026 | 0.0024 | 0.0014 | 0.0105 | 0.0035 |
Endosome | 0.0188 | 0.0267 | 0.0274 | 0.0508 | 0.0479 | 0.0488 | 0.0614 | 0.0766 | 0.0633 | 0.1238 | 0.0751 | 0.0554 | 0.067 | 0.0373 | 0.0649 | 0.0643 | 0.0108 | 0.0827 |
Golgi | 0.0004 | 0.0006 | 0.0008 | 0.0014 | 0.0007 | 0.0007 | 0.0048 | 0.0017 | 0.0023 | 0.0031 | 0.0042 | 0.0016 | 0.0037 | 0.001 | 0.0026 | 0.0007 | 0.001 | 0.0011 |
Lipid Particles | 0.0015 | 0.003 | 0.006 | 0.0031 | 0.0008 | 0.0027 | 0.011 | 0.0041 | 0.004 | 0.0056 | 0.0058 | 0.0041 | 0.0135 | 0.0082 | 0.0063 | 0.0011 | 0.0138 | 0.0046 |
Mitochondria | 0.0006 | 0.0013 | 0.0035 | 0.0037 | 0.0022 | 0.0016 | 0.0135 | 0.0042 | 0.0058 | 0.0109 | 0.0068 | 0.0025 | 0.0013 | 0.0075 | 0.0039 | 0.001 | 0.0019 | 0.0018 |
None | 0.8238 | 0.7437 | 0.7837 | 0.6322 | 0.6607 | 0.5623 | 0.5841 | 0.4728 | 0.5912 | 0.4482 | 0.5553 | 0.5108 | 0.654 | 0.682 | 0.6025 | 0.6754 | 0.7472 | 0.4956 |
Nuclear Periphery | 0.0012 | 0.0011 | 0.0026 | 0.0022 | 0.0019 | 0.0033 | 0.0047 | 0.002 | 0.0026 | 0.003 | 0.006 | 0.0028 | 0.0013 | 0.0035 | 0.003 | 0.0015 | 0.0023 | 0.0032 |
Nucleolus | 0.0006 | 0.0019 | 0.0022 | 0.0016 | 0.0006 | 0.0023 | 0.0024 | 0.0034 | 0.0021 | 0.0021 | 0.0025 | 0.0018 | 0.0003 | 0.0011 | 0.0008 | 0.0005 | 0.0017 | 0.0014 |
Nucleus | 0.0018 | 0.0041 | 0.0037 | 0.0056 | 0.0034 | 0.0147 | 0.0064 | 0.0071 | 0.0058 | 0.0055 | 0.0063 | 0.0095 | 0.0011 | 0.0033 | 0.0023 | 0.0019 | 0.0038 | 0.0102 |
Peroxisomes | 0.0003 | 0.0005 | 0.0015 | 0.0014 | 0.0002 | 0.0009 | 0.0058 | 0.0007 | 0.002 | 0.0183 | 0.0042 | 0.0016 | 0.0046 | 0.001 | 0.0065 | 0.0004 | 0.0011 | 0.0011 |
Punctate Nuclear | 0.0012 | 0.0011 | 0.0044 | 0.0034 | 0.0009 | 0.0036 | 0.0067 | 0.002 | 0.0052 | 0.0035 | 0.0056 | 0.0061 | 0.0008 | 0.0005 | 0.0036 | 0.0008 | 0.0028 | 0.0015 |
Vacuole | 0.0198 | 0.0536 | 0.03 | 0.0286 | 0.0618 | 0.084 | 0.0642 | 0.0902 | 0.0579 | 0.1241 | 0.0523 | 0.0824 | 0.0689 | 0.0619 | 0.0633 | 0.1323 | 0.006 | 0.0987 |
Vacuole Periphery | 0.0013 | 0.0036 | 0.0028 | 0.0033 | 0.0076 | 0.0062 | 0.0058 | 0.0054 | 0.0066 | 0.0222 | 0.0065 | 0.0052 | 0.0054 | 0.005 | 0.0091 | 0.013 | 0.0005 | 0.0084 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.0473 | 8.3998 | 8.9043 | 16.8837 | 10.0073 | 10.7328 | 13.8012 | 16.5825 | 15.602 | 15.6647 |
Translational Efficiency | 0.8296 | 0.7497 | 0.6685 | 0.3883 | 0.4815 | 0.8253 | 0.7016 | 0.5115 | 0.5444 | 0.5779 |
Dataset | Hit |
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Protein Abundance | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |