ORF
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.95 0.96 0.96 0.91 0.9 0.88 0.87 0.86 0.83 0.8 0.8 0.99 0.99 0.99 0.75 0.79 0.75 0.34 0.54 0.28 0.38 0.4 0.43
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0 0 0.15 0.2 0.17 0.29 0.27 0.32 0.23 0 0 0 0 0 0 0 0 0.05 0.09 0.07 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0 0.06 0 0.16 0.06 0 0 0.06 0.07 0.06 0.08 0 0 0 0.28 0.26 0.25 0.6 0.4 0.55 0.48 0.44 0.44
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 0 1 2
Bud 0 1 1 3 1 7 18 11 18 22 26 19 0 0 0 0 0 0 0 1 6 0 0 1
Bud Neck 0 0 0 0 1 0 0 0 1 0 0 1 0 1 0 0 0 0 1 0 3 0 2 1
Bud Site 0 0 0 0 0 0 0 1 0 1 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 0 1 0 0 1 1 0 2 1 0 1 0 0 0 0 0 1
Cytoplasm 214 251 403 339 384 628 775 788 508 519 588 735 154 195 195 165 131 152 88 150 114 48 104 144
Endoplasmic Reticulum 1 2 3 0 0 0 1 1 0 0 1 0 1 2 0 6 5 10 0 0 7 1 5 6
Endosome 0 1 1 2 7 2 4 5 0 3 3 17 0 0 0 1 2 2 3 2 11 0 3 4
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3 1 0 0
Mitochondria 4 15 7 9 10 101 177 157 171 168 232 211 0 0 0 4 7 3 3 4 18 11 19 12
Nucleus 0 0 0 2 1 0 5 13 2 4 6 2 1 0 1 10 3 5 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 6 5 8 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 1 0 0 1 0 0 5 1 2 1 5 1 0 0 0 0 0 0 0 0 1 0 0 0
Peroxisomes 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 2 3 3 9 0 4 9 8 0 0 0 0 0 0 1 1 1 0 1 3
Vac/Vac Membrane 16 1 23 9 67 44 34 32 35 43 41 76 0 0 0 62 43 51 153 112 226 61 114 150
Unique Cell Count 226 263 418 353 423 694 885 905 594 624 731 917 156 197 196 221 166 203 257 279 411 128 259 339
Labelled Cell Count 236 272 440 365 474 792 1027 1026 738 766 911 1074 157 198 198 250 191 225 257 279 411 128 259 339


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.5 4.2 4.0 4.1 3.3 3.5 3.7 3.3 3.3 3.3 3.3 4.7 4.6 4.7 5.3 5.2 5.0 4.4 4.6 4.8
Std Deviation (1e-4) 0.6 0.9 2.2 2.0 1.6 1.4 1.8 1.3 1.3 1.2 1.1 0.9 1.1 1.1 0.8 1.0 1.0 1.0 1.2 1.3 1.2
Intensity Change (Log2) – – – -0.08 -0.02 -0.35 -0.27 -0.19 -0.36 -0.32 -0.36 -0.33 0.18 0.15 0.16 0.33 0.31 0.26 0.07 0.15 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 8.0 7.0 7.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.5241 -0.9063 -1.0444 -1.147 -1.078 -0.9718 0.0873 0.8072 0.5829 0.6357 0.4572 0.7114 -1.0151 -0.2559 -0.6135 -0.4699 0.3574 -0.4379
Actin 0.0037 0.0001 0.0002 0.0001 0.0001 0.0003 0.0195 0.0001 0.0022 0.0076 0.0137 0.0041 0.001 0 0.0125 0.0002 0.0015 0.0005
Bud 0.0002 0.0002 0.0007 0.0064 0.0004 0.0003 0.0011 0.0033 0.0015 0.0029 0.0038 0.0009 0.0001 0.0002 0.0004 0.0002 0.0001 0.0007
Bud Neck 0.0004 0.0051 0.0003 0.0008 0.0003 0.0032 0.0039 0.0015 0.0009 0.0007 0.0012 0.005 0.0001 0.0003 0.0003 0.0001 0.0008 0.0022
Bud Periphery 0.0003 0.0004 0.0007 0.0104 0.0007 0.0005 0.0023 0.0122 0.0028 0.0059 0.0043 0.0019 0.0001 0.0003 0.0005 0.0002 0.0005 0.0007
Bud Site 0.0016 0.0033 0.002 0.0084 0.0003 0.0004 0.0044 0.0171 0.004 0.0008 0.0016 0.0007 0.0002 0.0006 0.0024 0.0003 0.0011 0.0075
Cell Periphery 0.0002 0.0005 0.0002 0.0004 0.0002 0.0003 0.0009 0.0013 0.0004 0.0004 0.0005 0.0005 0.0002 0.0006 0.0002 0.0001 0.0017 0.0007
Cytoplasm 0.1005 0.1168 0.0906 0.1493 0.1883 0.217 0.1269 0.2125 0.1826 0.1184 0.1643 0.2397 0.1422 0.1594 0.1706 0.0864 0.1714 0.2226
Cytoplasmic Foci 0.0192 0.0306 0.0341 0.0844 0.0175 0.0441 0.0588 0.0623 0.0547 0.0907 0.0721 0.0608 0.0319 0.0234 0.0417 0.0177 0.0181 0.0513
Eisosomes 0.0003 0.0001 0.0003 0.0001 0.0001 0.0001 0.0007 0.0001 0.0002 0.0002 0.0055 0.0001 0.0002 0.0002 0.0001 0.0003 0.0013 0.0001
Endoplasmic Reticulum 0.0023 0.0016 0.0022 0.0021 0.0033 0.003 0.0106 0.0194 0.0021 0.0022 0.0023 0.0026 0.0022 0.0026 0.0024 0.0014 0.0105 0.0035
Endosome 0.0188 0.0267 0.0274 0.0508 0.0479 0.0488 0.0614 0.0766 0.0633 0.1238 0.0751 0.0554 0.067 0.0373 0.0649 0.0643 0.0108 0.0827
Golgi 0.0004 0.0006 0.0008 0.0014 0.0007 0.0007 0.0048 0.0017 0.0023 0.0031 0.0042 0.0016 0.0037 0.001 0.0026 0.0007 0.001 0.0011
Lipid Particles 0.0015 0.003 0.006 0.0031 0.0008 0.0027 0.011 0.0041 0.004 0.0056 0.0058 0.0041 0.0135 0.0082 0.0063 0.0011 0.0138 0.0046
Mitochondria 0.0006 0.0013 0.0035 0.0037 0.0022 0.0016 0.0135 0.0042 0.0058 0.0109 0.0068 0.0025 0.0013 0.0075 0.0039 0.001 0.0019 0.0018
None 0.8238 0.7437 0.7837 0.6322 0.6607 0.5623 0.5841 0.4728 0.5912 0.4482 0.5553 0.5108 0.654 0.682 0.6025 0.6754 0.7472 0.4956
Nuclear Periphery 0.0012 0.0011 0.0026 0.0022 0.0019 0.0033 0.0047 0.002 0.0026 0.003 0.006 0.0028 0.0013 0.0035 0.003 0.0015 0.0023 0.0032
Nucleolus 0.0006 0.0019 0.0022 0.0016 0.0006 0.0023 0.0024 0.0034 0.0021 0.0021 0.0025 0.0018 0.0003 0.0011 0.0008 0.0005 0.0017 0.0014
Nucleus 0.0018 0.0041 0.0037 0.0056 0.0034 0.0147 0.0064 0.0071 0.0058 0.0055 0.0063 0.0095 0.0011 0.0033 0.0023 0.0019 0.0038 0.0102
Peroxisomes 0.0003 0.0005 0.0015 0.0014 0.0002 0.0009 0.0058 0.0007 0.002 0.0183 0.0042 0.0016 0.0046 0.001 0.0065 0.0004 0.0011 0.0011
Punctate Nuclear 0.0012 0.0011 0.0044 0.0034 0.0009 0.0036 0.0067 0.002 0.0052 0.0035 0.0056 0.0061 0.0008 0.0005 0.0036 0.0008 0.0028 0.0015
Vacuole 0.0198 0.0536 0.03 0.0286 0.0618 0.084 0.0642 0.0902 0.0579 0.1241 0.0523 0.0824 0.0689 0.0619 0.0633 0.1323 0.006 0.0987
Vacuole Periphery 0.0013 0.0036 0.0028 0.0033 0.0076 0.0062 0.0058 0.0054 0.0066 0.0222 0.0065 0.0052 0.0054 0.005 0.0091 0.013 0.0005 0.0084

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.0473 8.3998 8.9043 16.8837 10.0073 10.7328 13.8012 16.5825 15.602 15.6647
Translational Efficiency 0.8296 0.7497 0.6685 0.3883 0.4815 0.8253 0.7016 0.5115 0.5444 0.5779

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘