Standard name
Human Ortholog
Description NAD(+)-dependent glutamate synthase (GOGAT); synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source; assembles into filaments as cells approach stationary phase and under cytosolic acidification and starvation conditions
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0 0.06 0.08 – 0.09 0.11 0 0 0 0 0 0 – 0 0 0 0 0
Bud – – 0.05 0 0 0 0 0 0 – 0 0 0 0 0.06 0 0 0 – 0 0 0 0 0.06
Bud Neck – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 0 0 0.06 0.05 0.06 0 0 – 0.09 0.09 0 0 0 0 0 0 – 0 0 0 0 0
Cytoplasm – – 0.83 0.86 0.81 0.78 0.69 0.64 0.63 – 0.59 0.49 0.69 0.73 0.8 0.6 0.62 0.67 – 0 0.82 0.76 0.52 0.47
Endoplasmic Reticulum – – 0.09 0.1 0.15 0.17 0.19 0.19 0.23 – 0.13 0.21 0.05 0.06 0.05 0.05 0 0.05 – 0 0.07 0.07 0.1 0.1
Endosome – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi – – 0 0 0 0 0.08 0.08 0.06 – 0.09 0.11 0 0.09 0 0.16 0.2 0.11 – 0 0 0 0.08 0
Mitochondria – – 0 0 0 0 0 0 0.09 – 0.14 0.1 0 0 0 0 0 0 – 0 0 0 0 0
Nucleus – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 1.0 0 0 0 0
Nuclear Periphery – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus – – 0 0 0 0 0 0 0 – 0 0 0.19 0.1 0 0.08 0.11 0.08 – 0 0 0 0 0.05
Peroxisomes – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0.08 0.13
Vac/Vac Membrane – – 0.05 0 0 0 0 0 0 – 0 0.07 0 0 0.05 0.17 0.1 0.13 – 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 2 5 5 20 17 33 30 – 29 46 3 14 24 4 0 0 – 0 2 4 15 10
Bud – – 20 8 5 12 12 11 6 – 10 18 8 5 34 6 1 4 – 0 7 12 16 36
Bud Neck – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 – 0 0 2 8 14
Bud Site – – 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 – – – – – –
Cell Periphery – – 12 9 25 23 35 20 14 – 27 37 14 9 12 3 10 2 – 0 0 1 0 0
Cytoplasm – – 367 280 357 392 385 379 245 – 188 211 409 383 474 351 268 348 – 0 453 421 285 307
Endoplasmic Reticulum – – 39 31 65 86 105 110 89 – 41 92 31 29 27 30 17 24 – 0 39 40 57 63
Endosome – – 0 0 0 0 0 0 0 – 0 0 0 2 3 0 0 0 – 0 2 7 16 19
Golgi – – 6 7 11 8 44 50 25 – 30 47 25 47 23 92 85 57 – 0 6 13 41 23
Mitochondria – – 1 4 0 8 8 6 36 – 43 44 23 6 8 17 18 17 – 0 2 1 2 6
Nucleus – – 0 3 0 0 0 1 0 – 0 0 0 0 1 6 2 3 – 0 1 2 0 0
Nuclear Periphery – – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 1 1 1 2
Nucleolus – – 4 3 2 3 4 4 2 – 2 9 115 53 17 49 46 42 – 0 0 3 14 30
Peroxisomes – – 0 0 0 0 0 0 0 – 0 0 1 0 0 0 1 1 – 0 2 4 15 20
SpindlePole – – 0 0 0 0 0 0 0 – 0 0 2 7 2 2 2 3 – 0 3 12 42 82
Vac/Vac Membrane – – 22 0 2 2 8 25 3 – 7 30 11 10 29 97 43 66 – 0 24 14 15 16
Unique Cell Count 441 326 440 505 556 592 388 316 433 591 526 592 585 434 519 1 555 555 548 654
Labelled Cell Count 473 350 472 554 618 639 450 377 534 642 565 655 657 493 568 1 555 555 548 654


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – 50.2 49.8 53.3 49.8 50.0 47.4 47.0 – 46.0 45.3 53.3 41.8 32.7 64.8 74.1 66.4 57.9 63.2 62.9
Std Deviation (1e-4) – – 12.6 11.8 12.7 13.2 12.7 12.7 14.2 – 15.0 14.4 11.8 9.9 8.1 24.7 26.1 24.9 14.6 17.2 16.8
Intensity Change (Log2) – – – -0.01 0.09 -0.01 -0.01 -0.08 -0.1 – -0.13 -0.15 0.09 -0.26 -0.62 0.37 0.56 0.4 0.21 0.33 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 3.6 3.0 4.5 5.4 – 6.0 6.6 0 2.7 3.7 0 0 0
Bud -1.5 -3.0 -1.8 -2.1 -2.5 -2.5 – -1.0 -0.3 -3.1 -3.5 0.9 -3.5 -4.2 -3.7
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 2.2 1.5 2.6 0.6 0.7 – 3.6 3.7 -0.4 -1.1 -0.7 -2.9 -0.4 -3.0
Cytoplasm 1.0 -0.8 -2.2 -5.1 -6.8 -6.6 – -7.3 -10.8 -5.2 -3.9 -1.3 -8.0 -7.1 -5.7
Endoplasmic Reticulum 0.3 2.7 3.7 4.5 4.4 5.6 – 1.8 5.1 -2.3 -2.0 -2.8 -2.4 -3.0 -2.6
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0.8 1.2 0.3 4.7 5.0 3.8 – 5.2 5.9 2.7 5.2 2.4 7.8 8.8 6.0
Mitochondria 0 0 0 0 0 6.3 – 7.8 6.6 3.9 0 0 3.2 4.0 3.5
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 1.4 9.2 6.0 2.2 5.4 6.2 5.2
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole -4.1 -4.1 -4.5 -3.3 -0.6 -3.5 – -2.0 1.2 -2.8 -2.7 -0.1 5.7 2.8 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 36.0082 75.3924 67.5519 49.1101 55.9378 53.1203 37.5103 79.9334 70.6491 63.3838 58.9011 67.3954 49.3138 94.9088 75.9952 71.0519 69.2625 73.3492
Actin 0.0232 0.001 0.0016 0.0044 0.001 0.0056 0.0099 0.0015 0.0011 0.0052 0.0132 0.0024 0.0152 0.0016 0.0023 0.0001 0.0053 0.0014
Bud 0.0094 0.0128 0.0029 0.0375 0.0008 0.0015 0.0238 0.0159 0.0112 0.0116 0.0328 0.0155 0.0115 0.0151 0.0086 0.0046 0.0118 0.01
Bud Neck 0.0023 0.0113 0.0113 0.0018 0.0007 0.0034 0.0012 0.0077 0.0046 0.0046 0.0189 0.0495 0.002 0.0086 0.0215 0.005 0.0385 0.0208
Bud Periphery 0.0076 0.0034 0.001 0.0094 0.0007 0.0017 0.0058 0.0035 0.0028 0.0028 0.0094 0.0081 0.0036 0.0037 0.0029 0.0014 0.0085 0.0025
Bud Site 0.0386 0.0409 0.0144 0.0637 0.0029 0.0016 0.0294 0.0438 0.0223 0.0365 0.0143 0.0059 0.0271 0.0907 0.026 0.0108 0.031 0.0063
Cell Periphery 0.0058 0.0109 0.0049 0.004 0.0007 0.0026 0.0122 0.0126 0.0076 0.012 0.0051 0.0077 0.0084 0.0085 0.0065 0.0023 0.0046 0.0045
Cytoplasm 0.212 0.4693 0.4628 0.2373 0.3594 0.2923 0.3892 0.3933 0.3719 0.4213 0.2656 0.4968 0.3351 0.383 0.368 0.4322 0.2669 0.3153
Cytoplasmic Foci 0.2071 0.098 0.1389 0.2842 0.1185 0.155 0.1749 0.2198 0.1879 0.1873 0.4236 0.0947 0.2863 0.2498 0.2858 0.283 0.3235 0.3818
Eisosomes 0.0009 0.0006 0.001 0.0004 0.0002 0.0004 0.0015 0.0007 0.0004 0 0.0006 0.0003 0.0007 0.0004 0.0004 0 0.0003 0.0001
Endoplasmic Reticulum 0.0193 0.0024 0.0051 0.0017 0.0024 0.0081 0.0149 0.0053 0.0024 0.0106 0.0033 0.0092 0.0093 0.0006 0.0067 0.0011 0.0009 0.0044
Endosome 0.0285 0.0045 0.012 0.0126 0.0119 0.0177 0.0255 0.0022 0.0093 0.0116 0.0194 0.0225 0.0201 0.0049 0.0079 0.0144 0.0209 0.0232
Golgi 0.0043 0.0008 0.0015 0.0135 0.0017 0.0038 0.0042 0.0008 0.0017 0.0008 0.0075 0.0229 0.0075 0.002 0.0023 0.0007 0.0024 0.0096
Lipid Particles 0.0331 0.0068 0.01 0.0178 0.0519 0.0361 0.0192 0.0106 0.0106 0.0134 0.01 0.0051 0.0249 0.0104 0.011 0.0153 0.0319 0.0254
Mitochondria 0.1225 0.0832 0.084 0.2121 0.2246 0.1555 0.0455 0.0565 0.0758 0.0089 0.0389 0.035 0.0401 0.0398 0.0393 0.0284 0.0671 0.0505
None 0.0043 0.0038 0.001 0.0087 0.0156 0.0017 0.0161 0.0067 0.008 0.0054 0.0093 0.0071 0.0081 0.0039 0.0041 0.0005 0.0025 0.0025
Nuclear Periphery 0.067 0.0036 0.0082 0.0074 0.0054 0.0296 0.0418 0.0017 0.0035 0.0077 0.0129 0.002 0.0082 0.0004 0.0022 0.0015 0.0022 0.0022
Nucleolus 0.0561 0.141 0.1262 0.0259 0.0143 0.1664 0.0674 0.1389 0.1907 0.0922 0.0179 0.1079 0.0942 0.1088 0.124 0.141 0.0893 0.0707
Nucleus 0.0244 0.0118 0.0084 0.0072 0.0057 0.0352 0.0516 0.0174 0.0149 0.0064 0.0088 0.0131 0.0103 0.0118 0.0127 0.0068 0.0062 0.0094
Peroxisomes 0.0251 0.0077 0.0133 0.0049 0.0773 0.0201 0.0061 0.0081 0.017 0.012 0.0037 0.0059 0.0089 0.011 0.0145 0.0092 0.0264 0.0165
Punctate Nuclear 0.0021 0.0006 0.001 0.0018 0.0027 0.0079 0.0056 0.0046 0.0011 0.0017 0.0017 0.0008 0.0034 0.0009 0.0018 0.0024 0.0131 0.0018
Vacuole 0.0697 0.0697 0.0629 0.0341 0.0564 0.0235 0.0395 0.0392 0.0445 0.1172 0.0674 0.0604 0.0618 0.0381 0.0413 0.0325 0.0273 0.0331
Vacuole Periphery 0.0367 0.016 0.0276 0.0096 0.0454 0.0305 0.0145 0.0091 0.0108 0.0307 0.0157 0.0272 0.0132 0.0059 0.0102 0.0068 0.0195 0.0079

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 156.9069 154.8969 154.1631 168.2597 169.2158 117.508 141.7445 130.2235 121.1336 129.6625
Translational Efficiency 0.6568 0.6885 0.6367 0.5978 0.5705 0.6086 0.6014 0.4431 0.465 0.444

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description NAD(+)-dependent glutamate synthase (GOGAT); synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source; assembles into filaments as cells approach stationary phase and under cytosolic acidification and starvation conditions
Localization
Cell Percentages cytoplasm (83%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Glt1

Glt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Glt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available