Standard name
Description NAD(+)-dependent glutamate synthase (GOGAT); synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source; assembles into filaments as cells approach stationary phase and under cytosolic acidification and starvation conditions

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0.06 0.08 0.09 0.11 0 0 0 0 0 0 0 0 0 0 0
Bud 0.05 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.06 0.05 0.06 0 0 0.09 0.09 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.83 0.86 0.81 0.78 0.69 0.64 0.63 0.59 0.49 0.69 0.73 0.8 0.6 0.62 0.67 0 0.82 0.76 0.52 0.47
Endoplasmic Reticulum 0.09 0.1 0.15 0.17 0.19 0.19 0.23 0.13 0.21 0.05 0.06 0.05 0.05 0 0.05 0 0.07 0.07 0.1 0.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0.08 0.08 0.06 0.09 0.11 0 0.09 0 0.16 0.2 0.11 0 0 0 0.08 0
Mitochondria 0 0 0 0 0 0 0.09 0.14 0.1 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0.19 0.1 0 0.08 0.11 0.08 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.13
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0 0.07 0 0 0.05 0.17 0.1 0.13 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 5 5 20 17 33 30 29 46 3 14 24 4 0 0 0 2 4 15 10
Bud 20 8 5 12 12 11 6 10 18 8 5 34 6 1 4 0 7 12 16 36
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 8 14
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Cell Periphery 12 9 25 23 35 20 14 27 37 14 9 12 3 10 2 0 0 1 0 0
Cytoplasm 367 280 357 392 385 379 245 188 211 409 383 474 351 268 348 0 453 421 285 307
Endoplasmic Reticulum 39 31 65 86 105 110 89 41 92 31 29 27 30 17 24 0 39 40 57 63
Endosome 0 0 0 0 0 0 0 0 0 0 2 3 0 0 0 0 2 7 16 19
Golgi 6 7 11 8 44 50 25 30 47 25 47 23 92 85 57 0 6 13 41 23
Mitochondria 1 4 0 8 8 6 36 43 44 23 6 8 17 18 17 0 2 1 2 6
Nucleus 0 3 0 0 0 1 0 0 0 0 0 1 6 2 3 0 1 2 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 2
Nucleolus 4 3 2 3 4 4 2 2 9 115 53 17 49 46 42 0 0 3 14 30
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 0 2 4 15 20
SpindlePole 0 0 0 0 0 0 0 0 0 2 7 2 2 2 3 0 3 12 42 82
Vac/Vac Membrane 22 0 2 2 8 25 3 7 30 11 10 29 97 43 66 0 24 14 15 16
Unique Cell Count 441 326 440 505 556 592 388 316 433 591 526 592 585 434 519 1 555 555 548 654
Labelled Cell Count 473 350 472 554 618 639 450 377 534 642 565 655 657 493 568 1 555 555 548 654


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 50.2 49.8 53.3 49.8 50.0 47.4 47.0 46.0 45.3 53.3 41.8 32.7 64.8 74.1 66.4 57.9 63.2 62.9
Std Deviation (1e-4) 12.6 11.8 12.7 13.2 12.7 12.7 14.2 15.0 14.4 11.8 9.9 8.1 24.7 26.1 24.9 14.6 17.2 16.8
Intensity Change (Log2) -0.01 0.09 -0.01 -0.01 -0.08 -0.1 -0.13 -0.15 0.09 -0.26 -0.62 0.37 0.56 0.4 0.21 0.33 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 3.6 3.0 4.5 5.4 6.0 6.6 0 2.7 3.7 0 0 0
Bud -1.5 -3.0 -1.8 -2.1 -2.5 -2.5 -1.0 -0.3 -3.1 -3.5 0.9 -3.5 -4.2 -3.7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2.2 1.5 2.6 0.6 0.7 3.6 3.7 -0.4 -1.1 -0.7 -2.9 -0.4 -3.0
Cytoplasm 1.0 -0.8 -2.2 -5.1 -6.8 -6.6 -7.3 -10.8 -5.2 -3.9 -1.3 -8.0 -7.1 -5.7
Endoplasmic Reticulum 0.3 2.7 3.7 4.5 4.4 5.6 1.8 5.1 -2.3 -2.0 -2.8 -2.4 -3.0 -2.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.8 1.2 0.3 4.7 5.0 3.8 5.2 5.9 2.7 5.2 2.4 7.8 8.8 6.0
Mitochondria 0 0 0 0 0 6.3 7.8 6.6 3.9 0 0 3.2 4.0 3.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1.4 9.2 6.0 2.2 5.4 6.2 5.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.1 -4.1 -4.5 -3.3 -0.6 -3.5 -2.0 1.2 -2.8 -2.7 -0.1 5.7 2.8 4.1