Standard name
Human Ortholog
Description Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.95 0.96 0.85 0.77 0.72 0.68 0.67 0.64 0.63 0.98 0.99 0.98 0.99 0.98 0.97 0.96 0.87 0.92 0.9 0.89 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.17 0.25 0.21 0.36 0.41 0.45 0.42 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.05 0.07 0.15 0.15 0.12 0.09 0.13 0 0 0.05 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2
Bud 0 0 0 0 0 1 9 5 9 14 5 9 1 1 0 0 0 1 4 1 7 13 17 26
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 0 0 7 2 0 5 11 9 7 7 6 6 1 1 0 2 8 2 0 0 0 0 0 0
Cytoplasm 273 159 310 272 377 318 449 371 285 309 225 251 561 616 633 334 474 340 256 139 296 343 402 435
Endoplasmic Reticulum 1 0 4 2 0 3 4 6 0 2 1 2 0 1 0 8 21 5 0 1 4 1 2 1
Endosome 0 0 0 0 0 0 0 4 1 1 0 1 0 0 3 0 1 0 0 0 3 2 0 3
Golgi 0 1 0 0 1 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 1 1 0 0
Mitochondria 1 2 0 5 14 64 148 111 151 189 159 166 0 0 0 1 3 1 0 0 2 1 2 3
Nucleus 0 0 2 2 1 2 9 8 6 5 5 2 2 0 1 0 2 2 0 9 0 4 7 2
Nuclear Periphery 0 0 1 0 0 1 2 3 6 2 1 1 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 1 0 1 1 0 0 0 0 0 0 0 0 2 4 5
Vac/Vac Membrane 1 1 6 9 10 19 41 75 62 54 30 50 10 18 34 4 5 17 0 0 1 4 1 3
Unique Cell Count 276 160 318 285 393 372 584 517 418 463 350 397 571 624 649 337 486 351 267 160 324 382 453 492
Labelled Cell Count 276 163 330 292 404 413 673 594 528 584 432 491 576 638 671 349 516 368 267 160 324 382 453 492


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.6 11.5 12.3 11.1 9.8 8.3 7.4 6.6 6.0 5.6 5.2 4.9 11.4 12.2 11.2 35.0 36.6 31.9 12.0 11.6 12.2
Std Deviation (1e-4) 4.3 4.7 4.2 4.1 3.0 3.8 3.5 2.5 2.7 2.2 2.3 2.2 4.6 5.0 4.5 11.9 11.4 12.0 4.3 4.5 4.7
Intensity Change (Log2) -0.16 -0.33 -0.57 -0.73 -0.89 -1.03 -1.13 -1.25 -1.33 -0.11 -0.02 -0.14 1.51 1.57 1.37 -0.04 -0.09 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 3.1 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 -0.9 -0.3 -0.5 -0.5 -0.7 -0.5 -0.7 0 0 0 0 -0.6 0
Cytoplasm -1.4 -1.1 -5.5 -8.1 -9.3 -10.0 -10.4 -10.7 -11.1 0.8 1.4 0 1.6 0 -0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 1.1 2.4 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.4 7.8 9.8 8.9 12.0 13.1 13.8 13.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.0 0.6 2.3 3.3 6.0 6.0 5.0 3.8 5.3 -0.1 0.9 2.5 0 0 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.2367 15.9526 13.7791 11.8439 10.7267 14.126 10.0921 16.4977 13.748 10.2234 9.5411 12.8888 8.3841 11.2029 8.8128 9.3044 9.8114 9.245
Actin 0.0235 0.0029 0.008 0.049 0.0391 0.0032 0.0508 0.0001 0.0099 0.016 0.0321 0.0114 0.0176 0.0008 0.0144 0.0307 0.0003 0.0007
Bud 0.0039 0.0005 0.0043 0.0014 0.0003 0.0007 0.0015 0.0001 0.0006 0.0032 0.0027 0.0005 0.0006 0.0019 0.0022 0.0025 0.0001 0.0007
Bud Neck 0.0017 0.0003 0.0014 0.0007 0.001 0.0011 0.0023 0.0001 0.0012 0.0013 0.001 0.0011 0.0047 0.0003 0.0009 0.0019 0.0006 0.0007
Bud Periphery 0.0023 0.0001 0.003 0.001 0.0004 0.0003 0.0014 0 0.0007 0.003 0.0036 0.0013 0.0005 0.0009 0.0015 0.0031 0 0.0005
Bud Site 0.004 0.0012 0.0021 0.0009 0.0009 0.0001 0.0147 0.0001 0.0014 0.0081 0.0029 0.0002 0.0008 0.0064 0.0096 0.006 0 0.0001
Cell Periphery 0.0002 0 0.0001 0.0001 0.0001 0 0.0007 0 0.0001 0.0008 0.0002 0.0001 0.0001 0.0001 0.0002 0.0005 0 0
Cytoplasm 0.8713 0.9803 0.9239 0.7736 0.7622 0.9438 0.7793 0.9936 0.9142 0.802 0.6889 0.9311 0.8625 0.9651 0.8983 0.791 0.9123 0.9502
Cytoplasmic Foci 0.0207 0.003 0.0055 0.024 0.0111 0.0055 0.0202 0.0006 0.0098 0.01 0.0239 0.0024 0.0149 0.0021 0.0127 0.0336 0.0022 0.0084
Eisosomes 0.0001 0 0 0.0003 0.0005 0 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0003 0.0003 0 0
Endoplasmic Reticulum 0.0036 0.0006 0.0035 0.0047 0.0066 0.0036 0.011 0.0002 0.002 0.006 0.0028 0.0053 0.0033 0.0007 0.0029 0.0016 0.0006 0.0022
Endosome 0.011 0.0011 0.0055 0.0209 0.03 0.0043 0.0378 0.0001 0.0178 0.0567 0.1227 0.0128 0.0187 0.0004 0.015 0.0215 0.0009 0.0108
Golgi 0.0034 0.0008 0.0009 0.0121 0.0138 0.0005 0.0094 0 0.0062 0.0251 0.0796 0.0048 0.0053 0.0001 0.0057 0.0199 0.0001 0.0027
Lipid Particles 0.0106 0 0.0011 0.0094 0.0503 0.0008 0.0093 0 0.0036 0.0031 0.0066 0.0007 0.008 0 0.0064 0.0113 0 0.0023
Mitochondria 0.0042 0.0002 0.0008 0.0032 0.0241 0.0006 0.0066 0 0.0129 0.018 0.0158 0.0044 0.0024 0.0001 0.0028 0.038 0.0767 0.0004
None 0.0074 0.0017 0.0091 0.0023 0.0021 0.0018 0.0054 0.0007 0.0019 0.0016 0.0018 0.001 0.0154 0.011 0.0055 0.0022 0.0015 0.0037
Nuclear Periphery 0.0037 0.0009 0.0045 0.0065 0.0092 0.0077 0.0112 0.0004 0.002 0.0047 0.002 0.0083 0.0098 0.0012 0.0016 0.0015 0.0006 0.0026
Nucleolus 0.0002 0 0.0002 0.0001 0.0011 0.0001 0.001 0 0.0001 0.0003 0.0001 0.0001 0.0004 0 0.0001 0.0002 0 0
Nucleus 0.0122 0.0051 0.02 0.0422 0.0179 0.0218 0.0137 0.0037 0.0076 0.0215 0.0033 0.0116 0.0199 0.0074 0.0138 0.0192 0.0035 0.0109
Peroxisomes 0.006 0.0003 0.0018 0.0398 0.0167 0.0002 0.0059 0 0.0029 0.0009 0.0028 0.0002 0.0079 0 0.0035 0.0113 0.0001 0.0009
Punctate Nuclear 0.0086 0.0006 0.0028 0.0057 0.0072 0.0028 0.0104 0.0001 0.0011 0.0008 0.0008 0.0008 0.0022 0.0005 0.001 0.0012 0.0002 0.0009
Vacuole 0.0012 0.0004 0.0011 0.001 0.0036 0.0007 0.0053 0.0001 0.0029 0.0132 0.0035 0.0009 0.0031 0.0008 0.0012 0.0018 0.0002 0.0008
Vacuole Periphery 0.0004 0.0001 0.0002 0.0009 0.0016 0.0004 0.0018 0 0.0011 0.0038 0.0027 0.0011 0.0017 0.0001 0.0003 0.0007 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 53.6971 50.1259 59.8912 58.7017 53.6017 46.5434 66.1447 57.3984 80.1999 55.7824
Translational Efficiency 3.4591 3.5174 2.5956 2.9207 2.7857 3.8826 2.4267 2.4877 1.8508 2.2906

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1187 370 2624 1412 1431 192 242 1285 2618 562 2866 2697

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 907.04 1205.97 1140.29 1709.08 789.67 1713.70 1375.15 1803.36 842.89 1379.43 1160.12 1754.00
Standard Deviation 207.44 345.72 286.43 722.33 168.04 418.84 414.29 756.17 195.86 443.40 306.39 740.15
Intensity Change Log 2 0.410956 0.330163 0.913982 1.117793 0.800267 1.191366 0.783065 0.568071 1.049734

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000197 0.000224 0.000121 0.001723 0.000152 0.000479 0.000580 0.002705 0.000172 0.000311 0.000160 0.002191
Bud Neck 0.003556 0.001321 0.005475 0.003564 0.003607 0.002662 0.003244 0.003615 0.003584 0.001779 0.005287 0.003589
Bud Site 0.006058 0.006286 0.008555 0.018783 0.005766 0.012967 0.007819 0.019272 0.005898 0.008568 0.008493 0.019016
Cell Periphery 0.000131 0.000067 0.000079 0.000555 0.000144 0.000220 0.000369 0.000253 0.000138 0.000119 0.000103 0.000411
Cytoplasm 0.722597* 0.841790* 0.628556* 0.675678* 0.723153* 0.787634* 0.515559* 0.692594* 0.722901* 0.823288* 0.619015* 0.683738*
Cytoplasmic Foci 0.123538 0.041877 0.003422 0.014768 0.103898 0.048534 0.021413 0.018287 0.112803 0.044151 0.004941 0.016445
Eisosomes 0.000054 0.000011 0.000008 0.000019 0.000043 0.000010 0.000073 0.000014 0.000048 0.000011 0.000014 0.000016
Endoplasmic Reticulum 0.001312 0.000505 0.000752 0.001019 0.001401 0.000408 0.003778 0.000517 0.001361 0.000472 0.001008 0.000780
Endosome 0.008223 0.002690 0.000577 0.005649 0.005443 0.009302 0.005784 0.011358 0.006704 0.004949 0.001017 0.008369
Golgi 0.002452 0.000401 0.000076 0.011562 0.000657 0.001576 0.000301 0.012718 0.001471 0.000802 0.000095 0.012113
Lipid Particles 0.003038 0.000647 0.000306 0.001499 0.002546 0.001092 0.003264 0.001171 0.002769 0.000799 0.000556 0.001343
Mitochondria 0.002299 0.000111 0.000595 0.004023 0.001981 0.000119 0.000344 0.005196 0.002125 0.000114 0.000574 0.004582
Mitotic Spindle 0.002188 0.007243 0.009207 0.040956 0.002753 0.017510 0.011035 0.054183 0.002497 0.010750 0.009362 0.047258
None 0.003693 0.009150 0.010762 0.008836 0.009307 0.003886 0.009132 0.009440 0.006761 0.007352 0.010624 0.009124
Nuclear Periphery 0.000295 0.000091 0.000859 0.000685 0.000243 0.000146 0.001397 0.000543 0.000267 0.000110 0.000904 0.000618
Nuclear Periphery Foci 0.002234 0.002223 0.000505 0.002954 0.001838 0.003222 0.006002 0.002462 0.002018 0.002564 0.000969 0.002720
Nucleolus 0.000557 0.000364 0.000236 0.000292 0.000556 0.000280 0.000711 0.000325 0.000556 0.000335 0.000276 0.000308
Nucleus 0.059804 0.059241 0.305639* 0.152131* 0.093686 0.066761 0.239617* 0.100486 0.078324 0.061810 0.300065* 0.127524
Peroxisomes 0.001665 0.000879 0.000075 0.000350 0.000857 0.000686 0.000324 0.002389 0.001223 0.000813 0.000096 0.001321
Vacuole 0.055172 0.024585 0.024077 0.052433 0.040634 0.041853 0.168972 0.052673 0.047226 0.030485 0.036312 0.052547
Vacuole Periphery 0.000936 0.000294 0.000115 0.002521 0.001332 0.000652 0.000282 0.009799 0.001153 0.000416 0.000129 0.005989

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.50 0.73 -3.19 -2.74 -3.30 -2.63 -8.01 -2.27 -1.15 -0.61 -3.15 -2.41 -3.78 -2.74 -3.54
Bud Neck 4.77 -2.78 -0.60 -7.58 2.77 0.74 -1.10 0.50 -0.47 1.66 4.01 -3.14 -0.07 -4.74 3.38
Bud Site -0.41 -0.92 -7.09 -5.63 -5.24 -2.05 -2.78 -4.05 0.56 -1.44 -1.99 -1.39 -7.82 -2.69 -4.81
Cell Periphery 4.71 6.03 -12.29 -13.66 -13.84 -1.40 -6.03 -6.48 -0.39 2.12 0.03 4.03 -13.71 -8.52 -15.28
Cytoplasm -8.65 18.48 11.40 17.95 -6.32 -1.59 16.83 8.73 6.21 -12.02 -8.07 25.95 14.46 17.60 -11.44
Cytoplasmic Foci 11.88 26.18 23.27 4.98 -15.70 8.99 20.70 23.14 5.10 0.90 14.54 37.36 32.86 6.80 -16.20
Eisosomes 9.91 10.80 9.18 -2.12 -2.87 12.25 -2.38 13.72 -0.93 8.29 14.78 14.51 15.02 -2.03 1.70
Endoplasmic Reticulum 2.46 0.68 2.62 -0.43 6.34 5.30 -4.03 6.01 0.34 6.79 4.53 0.32 5.66 -0.07 11.45
Endosome 6.30 11.05 8.37 1.12 -3.54 -0.13 2.17 3.57 0.99 1.79 2.47 14.27 8.44 1.22 -3.83
Golgi 2.66 3.09 -0.50 -2.57 -2.88 -0.87 2.92 -2.59 -1.73 -2.96 2.06 3.89 -1.97 -3.31 -4.24
Lipid Particles 5.09 6.71 0.54 -5.92 -9.60 3.04 -1.57 1.14 -2.25 2.46 6.11 8.59 1.03 -6.10 -10.18
Mitochondria 2.33 1.74 0.93 -2.18 -1.15 3.20 2.83 1.03 -2.54 -2.04 3.80 2.81 1.36 -3.27 -1.78
Mitotic Spindle -1.58 -6.30 -8.62 -4.33 -5.36 -2.49 -2.91 -8.61 -2.86 -5.66 -2.69 -6.76 -12.12 -5.39 -7.86
None -2.49 -13.91 -5.09 1.18 8.28 5.33 3.31 2.50 -5.18 -1.73 0.01 -4.33 0.56 0.25 8.30
Nuclear Periphery 2.87 -13.85 -5.98 -12.64 8.36 0.11 -13.56 -12.60 -11.72 9.76 3.40 -23.72 -10.86 -17.67 13.63
Nuclear Periphery Foci -0.93 4.98 -1.49 -0.08 -10.68 -3.70 -6.40 -2.39 2.51 5.47 -2.95 3.96 -2.84 1.34 -9.50
Nucleolus 1.12 1.70 1.88 0.55 2.03 3.22 1.50 5.01 0.30 1.79 1.74 2.83 3.30 0.60 2.67
Nucleus -0.60 -54.88 -23.64 -16.03 28.06 2.57 -11.30 -8.37 -7.73 8.36 2.37 -52.98 -21.81 -17.11 35.30
Peroxisomes 2.10 4.74 4.48 2.89 -2.89 1.37 3.80 0.64 -0.33 -1.24 2.15 6.46 3.60 1.44 -1.95
Vacuole 5.30 1.08 -13.66 -16.43 -15.03 -4.10 -13.29 -16.65 -5.17 6.82 -0.15 -6.85 -21.70 -15.66 -14.93
Vacuole Periphery 2.90 3.81 0.68 -1.93 -2.76 2.27 3.17 -1.00 -2.43 -2.87 3.60 4.79 -0.54 -3.06 -3.79