Standard name
Description Alpha subunit of COPI vesicle coatomer complex; complex surrounds transport vesicles in the early secretory pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.12 0.16 0.07 0.07 0.09 0.08 0.09 0.1 – 0.05 0.07 0.06 0.11 0.12 0.08 0.17 0.09 – 0 0 0.05 0 0.05 0.06
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0.11 0.05 0 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.1 0 0 0 0 0 0 – 0 0 0 0.07 0.08 0.06 0 0 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0.9 0.63 0.64 0.58 0.76 0.31 0.31 0.3 – 0.21 0.14 0.16 0.86 0.86 0.79 0.9 0.85 – 0.9 0.76 0.85 0.85 0.86 0.87
Mitochondria 0.35 0.5 0.58 0.81 0.53 0.84 0.86 0.85 – 0.86 0.91 0.91 0.1 0.11 0 0.07 0.13 – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.05 0.05 – 0.09 0.09 0.09 0 0 0 0 0.05 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0.12 0.08 0 0.1 0 0 0 – 0.06 0 0 0.17 0.18 0.24 0.12 0.16 – 0 0.12 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 67 52 24 13 31 21 28 25 – 15 18 24 26 27 19 7 9 – 13 11 16 7 15 21
Bud 3 3 5 1 2 2 1 4 – 4 7 10 2 2 0 0 0 – 1 2 2 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 0 – 1 1 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 1 1 0 0 1 0 1 – 0 1 1 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 13 15 12 3 10 7 10 8 – 6 5 7 9 9 26 2 4 – 1 7 1 0 0 1
Endoplasmic Reticulum 16 32 12 4 4 8 8 8 – 3 5 5 16 18 14 1 4 – 1 3 2 0 1 2
Endosome 0 2 1 0 1 0 1 0 – 0 1 0 0 0 1 0 0 – 5 11 7 8 3 3
Golgi 497 201 205 110 250 79 92 77 – 64 38 66 199 196 194 36 86 – 537 300 291 236 242 329
Mitochondria 194 161 186 153 173 212 254 217 – 265 245 363 24 24 10 3 13 – 13 9 8 8 6 6
Nucleus 0 2 0 0 0 1 1 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 – 2 2 5 0 0 0 0 0 – 1 0 0 0 0 0
Nucleolus 13 4 5 4 5 7 15 14 – 27 23 35 2 2 3 0 5 – 1 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 – 1 0 0 1 1 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0
Vac/Vac Membrane 77 40 27 6 34 10 13 9 – 19 6 15 40 41 60 5 16 – 17 46 4 11 7 3
Unique Cell Count 555 320 322 190 329 251 296 255 307 268 401 231 228 247 40 101 599 398 342 277 283 377
Labelled Cell Count 880 513 478 294 510 348 424 364 406 352 532 318 319 327 54 137 599 398 342 277 283 377


Early Golgi, Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 34.0 30.0 26.2 24.4 25.6 24.3 24.9 24.7 – 24.1 22.8 22.9 32.5 32.5 32.8 25.0 28.5 – 31.0 33.2 35.1
Std Deviation (1e-4) 6.0 8.2 6.3 5.2 5.7 6.1 6.5 6.7 – 6.0 6.4 6.1 7.9 7.9 9.5 5.7 9.7 – 6.4 9.6 7.1
Intensity Change (Log2) – – – -0.1 -0.03 -0.11 -0.07 -0.08 – -0.12 -0.2 -0.19 0.31 0.31 0.33 -0.07 0.12 – 0.24 0.34 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.3 0.9 0.4 0.9 1.0 – -1.3 -0.3 -0.8 1.5 1.7 0.1 0 0.5 –
Bud 0 0 0 0 0 – 0 0.9 0.9 0 0 0 0 0 –
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 –
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 –
Cytoplasm -1.4 -0.5 -0.6 -0.2 -0.4 – -1.3 -1.3 -1.7 0.1 0.1 3.2 0 0 –
Endoplasmic Reticulum -1.0 -2.1 -0.3 -0.7 -0.4 – -2.3 -1.3 -2.2 1.7 2.1 1.1 0 0 –
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 –
Golgi -1.3 3.4 -7.6 -8.1 -8.0 – -10.8 -12.2 -13.0 5.9 5.8 3.8 3.3 4.1 –
Mitochondria 5.3 -1.3 6.9 7.7 7.1 – 7.9 9.2 10.2 -11.3 -11.2 -13.4 -6.0 -7.9 –
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 –
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 –
Nucleolus 0 0 1.0 2.5 2.6 – 4.1 4.0 4.2 0 0 0 0 0 –
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 –
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 –
Vacuole -2.3 0.9 -2.1 -2.0 -2.4 – -1.1 -3.2 -2.7 3.2 3.4 5.2 0 2.2 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 24.5511 32.3995 30.975 29.9604 30.6707 31.2572 25.7041 33.4544 30.215 32.6029 28.9145 32.6361 27.3391 36.9467 34.2823 34.4152 31.5688 34.8757
Actin 0.0304 0.0092 0.0072 0.0149 0.0007 0.0036 0.0225 0.0102 0.0481 0.0029 0.0668 0.0021 0.0768 0.0142 0.0328 0.0242 0.0266 0.0019
Bud 0.0005 0.0008 0.0009 0.0004 0.0003 0.0001 0.0007 0.0005 0.0004 0 0.0002 0 0.0003 0.007 0.0005 0.0003 0.0003 0
Bud Neck 0.0009 0.0004 0.0002 0.0006 0.0002 0.0001 0.0025 0.0003 0.0007 0.0001 0.0003 0.0001 0.0005 0.0005 0.0002 0.0002 0.0002 0.0001
Bud Periphery 0.0006 0.0004 0.0014 0.0005 0.0004 0.0001 0.0012 0.0003 0.0006 0.0001 0.0004 0 0.0005 0.0107 0.0008 0.0007 0.0004 0.0001
Bud Site 0.0011 0.006 0.0005 0.0011 0.0001 0.0001 0.0043 0.0026 0.0009 0 0.0017 0 0.001 0.004 0.0008 0.0002 0.0002 0
Cell Periphery 0.0006 0.001 0.0008 0.0007 0.0009 0.0001 0.0006 0.0005 0.0005 0.0002 0.0001 0 0.0004 0.001 0.0004 0.0006 0.0002 0.0001
Cytoplasm 0.0081 0.0049 0.0045 0.0014 0.0014 0.0019 0.0023 0.0159 0.0038 0.0003 0.0003 0.0002 0.0013 0.0015 0.0006 0.0003 0.0005 0.0001
Cytoplasmic Foci 0.0265 0.017 0.0124 0.0136 0.0089 0.0098 0.0187 0.0132 0.0109 0.0043 0.0045 0.0032 0.0307 0.0087 0.0072 0.0049 0.0056 0.0026
Eisosomes 0.0007 0.0003 0.0001 0.0004 0 0.0001 0.0004 0.0001 0.0005 0.0002 0.0002 0 0.0013 0.0004 0.0002 0.001 0.0002 0.0001
Endoplasmic Reticulum 0.004 0.0014 0.0016 0.0011 0.0008 0.001 0.0035 0.0027 0.0048 0.0007 0.0012 0.0003 0.0013 0.001 0.001 0.001 0.0011 0.0005
Endosome 0.1036 0.1543 0.1441 0.228 0.1832 0.0782 0.1107 0.1152 0.1094 0.1709 0.0923 0.0477 0.1225 0.112 0.0733 0.0986 0.1369 0.048
Golgi 0.5416 0.6598 0.6649 0.6532 0.5292 0.8129 0.6026 0.6917 0.6652 0.6303 0.7188 0.9109 0.6341 0.7571 0.6853 0.7908 0.6615 0.8529
Lipid Particles 0.0231 0.0228 0.0178 0.0112 0.0398 0.0057 0.0362 0.003 0.0096 0.0013 0.003 0.0009 0.0084 0.0025 0.0196 0.0024 0.0216 0.007
Mitochondria 0.1722 0.046 0.0669 0.0296 0.0155 0.05 0.1193 0.0962 0.0821 0.0796 0.0414 0.0184 0.0842 0.0285 0.0924 0.0172 0.1234 0.0656
None 0.0062 0.0001 0.0018 0.0001 0 0.0013 0.0047 0.0002 0.0031 0 0.0002 0 0.0011 0.0001 0.0009 0 0.0001 0
Nuclear Periphery 0.0146 0.001 0.0035 0.0005 0.0014 0.0076 0.0131 0.005 0.004 0.0006 0.0358 0.0003 0.0012 0.001 0.0023 0.0004 0.0005 0.0008
Nucleolus 0.0021 0.0037 0.0045 0.0001 0.001 0.0005 0.002 0.001 0.0002 0 0.0001 0 0.0004 0.0003 0.0002 0.0001 0 0.0001
Nucleus 0.01 0.0007 0.0023 0.0001 0.0008 0.0037 0.0036 0.0013 0.0006 0.0001 0.0008 0 0.0004 0.0006 0.0003 0.0001 0.0001 0.0001
Peroxisomes 0.0073 0.0006 0.0005 0.0032 0.0002 0.0001 0.0028 0.0005 0.0049 0 0.0012 0 0.0009 0.0002 0.0022 0.0001 0.0002 0.0001
Punctate Nuclear 0.0192 0.0001 0.0017 0.0001 0 0.0044 0.0067 0.0012 0.0009 0 0.0002 0 0.0122 0 0.0007 0 0 0
Vacuole 0.0087 0.0241 0.0179 0.0136 0.0674 0.0033 0.01 0.0109 0.0119 0.0167 0.0028 0.0041 0.0044 0.0184 0.0156 0.0163 0.0049 0.0032
Vacuole Periphery 0.018 0.0454 0.0448 0.0254 0.1479 0.0155 0.0316 0.0275 0.0368 0.0918 0.0279 0.0118 0.0161 0.0306 0.0626 0.0405 0.0155 0.0166

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 227.6575 202.1442 185.9266 208.585 191.4227 194.5027 203.7655 201.2286 192.248 225.7676
Translational Efficiency 0.6167 0.6744 0.6405 0.5836 0.5629 0.6537 0.675 0.5124 0.5686 0.5101

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘