Standard name | |
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Description | Alpha subunit of COPI vesicle coatomer complex; complex surrounds transport vesicles in the early secretory pathway |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 67 | 52 | 24 | 13 | 31 | 21 | 28 | 25 | – | 15 | 18 | 24 | 26 | 27 | 19 | 7 | 9 | – | 13 | 11 | 16 | 7 | 15 | 21 |
Bud | 3 | 3 | 5 | 1 | 2 | 2 | 1 | 4 | – | 4 | 7 | 10 | 2 | 2 | 0 | 0 | 0 | – | 1 | 2 | 2 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 13 | 15 | 12 | 3 | 10 | 7 | 10 | 8 | – | 6 | 5 | 7 | 9 | 9 | 26 | 2 | 4 | – | 1 | 7 | 1 | 0 | 0 | 1 |
Endoplasmic Reticulum | 16 | 32 | 12 | 4 | 4 | 8 | 8 | 8 | – | 3 | 5 | 5 | 16 | 18 | 14 | 1 | 4 | – | 1 | 3 | 2 | 0 | 1 | 2 |
Endosome | 0 | 2 | 1 | 0 | 1 | 0 | 1 | 0 | – | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | – | 5 | 11 | 7 | 8 | 3 | 3 |
Golgi | 497 | 201 | 205 | 110 | 250 | 79 | 92 | 77 | – | 64 | 38 | 66 | 199 | 196 | 194 | 36 | 86 | – | 537 | 300 | 291 | 236 | 242 | 329 |
Mitochondria | 194 | 161 | 186 | 153 | 173 | 212 | 254 | 217 | – | 265 | 245 | 363 | 24 | 24 | 10 | 3 | 13 | – | 13 | 9 | 8 | 8 | 6 | 6 |
Nucleus | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 13 | 4 | 5 | 4 | 5 | 7 | 15 | 14 | – | 27 | 23 | 35 | 2 | 2 | 3 | 0 | 5 | – | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 1 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | 77 | 40 | 27 | 6 | 34 | 10 | 13 | 9 | – | 19 | 6 | 15 | 40 | 41 | 60 | 5 | 16 | – | 17 | 46 | 4 | 11 | 7 | 3 |
Unique Cell Count | 555 | 320 | 322 | 190 | 329 | 251 | 296 | 255 | 307 | 268 | 401 | 231 | 228 | 247 | 40 | 101 | 599 | 398 | 342 | 277 | 283 | 377 | ||
Labelled Cell Count | 880 | 513 | 478 | 294 | 510 | 348 | 424 | 364 | 406 | 352 | 532 | 318 | 319 | 327 | 54 | 137 | 599 | 398 | 342 | 277 | 283 | 377 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 34.0 | 30.0 | 26.2 | 24.4 | 25.6 | 24.3 | 24.9 | 24.7 | – | 24.1 | 22.8 | 22.9 | 32.5 | 32.5 | 32.8 | 25.0 | 28.5 | – | 31.0 | 33.2 | 35.1 |
Std Deviation (1e-4) | 6.0 | 8.2 | 6.3 | 5.2 | 5.7 | 6.1 | 6.5 | 6.7 | – | 6.0 | 6.4 | 6.1 | 7.9 | 7.9 | 9.5 | 5.7 | 9.7 | – | 6.4 | 9.6 | 7.1 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.03 | -0.11 | -0.07 | -0.08 | – | -0.12 | -0.2 | -0.19 | 0.31 | 0.31 | 0.33 | -0.07 | 0.12 | – | 0.24 | 0.34 | 0.42 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.3 | 0.9 | 0.4 | 0.9 | 1.0 | – | -1.3 | -0.3 | -0.8 | 1.5 | 1.7 | 0.1 | 0 | 0.5 | – |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0.9 | 0.9 | 0 | 0 | 0 | 0 | 0 | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cytoplasm | -1.4 | -0.5 | -0.6 | -0.2 | -0.4 | – | -1.3 | -1.3 | -1.7 | 0.1 | 0.1 | 3.2 | 0 | 0 | – |
Endoplasmic Reticulum | -1.0 | -2.1 | -0.3 | -0.7 | -0.4 | – | -2.3 | -1.3 | -2.2 | 1.7 | 2.1 | 1.1 | 0 | 0 | – |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Golgi | -1.3 | 3.4 | -7.6 | -8.1 | -8.0 | – | -10.8 | -12.2 | -13.0 | 5.9 | 5.8 | 3.8 | 3.3 | 4.1 | – |
Mitochondria | 5.3 | -1.3 | 6.9 | 7.7 | 7.1 | – | 7.9 | 9.2 | 10.2 | -11.3 | -11.2 | -13.4 | -6.0 | -7.9 | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nucleolus | 0 | 0 | 1.0 | 2.5 | 2.6 | – | 4.1 | 4.0 | 4.2 | 0 | 0 | 0 | 0 | 0 | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Vacuole | -2.3 | 0.9 | -2.1 | -2.0 | -2.4 | – | -1.1 | -3.2 | -2.7 | 3.2 | 3.4 | 5.2 | 0 | 2.2 | – |