TheCellVision

COP1

Standard name	COP1 ORF YDL145C Aliases coatomer subunit alpha, RET1, SEC33, SOO1
Human Ortholog	COPA
Description	Alpha subunit of COPI vesicle coatomer complex; complex surrounds transport vesicles in the early secretory pathway

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Scale images

cop1-1

Micrographs

Sub-cellular Localization

LOC-score
Cell count

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0.12	0.16	0.07	0.07	0.09	0.08	0.09	0.1	–	0.05	0.07	0.06	0.11	0.12	0.08	0.17	0.09	–	0	0	0.05	0	0.05	0.06
Bud	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0
Bud Neck	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	–	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0
Cytoplasm	0	0.05	0	0	0	0	0	0	–	0	0	0	0	0	0.11	0.05	0	–	0	0	0	0	0	0
Endoplasmic Reticulum	0	0.1	0	0	0	0	0	0	–	0	0	0	0.07	0.08	0.06	0	0	–	0	0	0	0	0	0
Endosome	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0
Golgi	0.9	0.63	0.64	0.58	0.76	0.31	0.31	0.3	–	0.21	0.14	0.16	0.86	0.86	0.79	0.9	0.85	–	0.9	0.76	0.85	0.85	0.86	0.87
Mitochondria	0.35	0.5	0.58	0.81	0.53	0.84	0.86	0.85	–	0.86	0.91	0.91	0.1	0.11	0	0.07	0.13	–	0	0	0	0	0	0
Nucleus	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0
Nuclear Periphery	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	0.05	0.05	–	0.09	0.09	0.09	0	0	0	0	0.05	–	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0
SpindlePole	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0
Vac/Vac Membrane	0.14	0.12	0.08	0	0.1	0	0	0	–	0.06	0	0	0.17	0.18	0.24	0.12	0.16	–	0	0.12	0	0	0	0

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	67	52	24	13	31	21	28	25	–	15	18	24	26	27	19	7	9	–	13	11	16	7	15	21
Bud	3	3	5	1	2	2	1	4	–	4	7	10	2	2	0	0	0	–	1	2	2	0	1	3
Bud Neck	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	1	0	–	1	1	0	0	0	0	0	0	–	–	–	–	–	–	–
Cell Periphery	0	1	1	0	0	1	0	1	–	0	1	1	0	0	0	0	0	–	0	0	0	0	0	0
Cytoplasm	13	15	12	3	10	7	10	8	–	6	5	7	9	9	26	2	4	–	1	7	1	0	0	1
Endoplasmic Reticulum	16	32	12	4	4	8	8	8	–	3	5	5	16	18	14	1	4	–	1	3	2	0	1	2
Endosome	0	2	1	0	1	0	1	0	–	0	1	0	0	0	1	0	0	–	5	11	7	8	3	3
Golgi	497	201	205	110	250	79	92	77	–	64	38	66	199	196	194	36	86	–	537	300	291	236	242	329
Mitochondria	194	161	186	153	173	212	254	217	–	265	245	363	24	24	10	3	13	–	13	9	8	8	6	6
Nucleus	0	2	0	0	0	1	1	0	–	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0
Nuclear Periphery	0	0	0	0	0	0	0	1	–	2	2	5	0	0	0	0	0	–	1	0	0	0	0	0
Nucleolus	13	4	5	4	5	7	15	14	–	27	23	35	2	2	3	0	5	–	1	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	0	–	0	0	1	0	0	0	0	0	–	1	0	0	1	1	0
SpindlePole	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	–	0	0	1	0	0	0
Vac/Vac Membrane	77	40	27	6	34	10	13	9	–	19	6	15	40	41	60	5	16	–	17	46	4	11	7	3
Unique Cell Count	555	320	322	190	329	251	296	255		307	268	401	231	228	247	40	101		599	398	342	277	283	377
Labelled Cell Count	880	513	478	294	510	348	424	364		406	352	532	318	319	327	54	137		599	398	342	277	283	377

Yeast GFP Assignment

Early Golgi, Golgi

(Huh et al. Nature 2003 425:686)

Protein Abundance

Screen	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	AF100	AF140	AF180
Mean Cell GFP Intensity (1e^-4)	34.0	30.0	26.2	24.4	25.6	24.3	24.9	24.7	–	24.1	22.8	22.9	32.5	32.5	32.8	25.0	28.5	–	31.0	33.2	35.1
Std Deviation (1e^-4)	6.0	8.2	6.3	5.2	5.7	6.1	6.5	6.7	–	6.0	6.4	6.1	7.9	7.9	9.5	5.7	9.7	–	6.4	9.6	7.1
Intensity Change (Log₂)	–	–	–	-0.1	-0.03	-0.11	-0.07	-0.08	–	-0.12	-0.2	-0.19	0.31	0.31	0.33	-0.07	0.12	–	0.24	0.34	0.42

Localization Change

Localization	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3
Cortical Patches	-0.3	0.9	0.4	0.9	1.0	–	-1.3	-0.3	-0.8	1.5	1.7	0.1	0	0.5	–
Bud	0	0	0	0	0	–	0	0.9	0.9	0	0	0	0	0	–
Bud Neck	0	0	0	0	0	–	0	0	0	0	0	0	0	0	–
Bud Site	0	0	0	0	0	–	0	0	0	0	0	0	0	0	–
Cell Periphery	0	0	0	0	0	–	0	0	0	0	0	0	0	0	–
Cytoplasm	-1.4	-0.5	-0.6	-0.2	-0.4	–	-1.3	-1.3	-1.7	0.1	0.1	3.2	0	0	–
Endoplasmic Reticulum	-1.0	-2.1	-0.3	-0.7	-0.4	–	-2.3	-1.3	-2.2	1.7	2.1	1.1	0	0	–
Endosome	0	0	0	0	0	–	0	0	0	0	0	0	0	0	–
Golgi	-1.3	3.4	-7.6	-8.1	-8.0	–	-10.8	-12.2	-13.0	5.9	5.8	3.8	3.3	4.1	–
Mitochondria	5.3	-1.3	6.9	7.7	7.1	–	7.9	9.2	10.2	-11.3	-11.2	-13.4	-6.0	-7.9	–
Nucleus	0	0	0	0	0	–	0	0	0	0	0	0	0	0	–
Nuclear Periphery	0	0	0	0	0	–	0	0	0	0	0	0	0	0	–
Nucleolus	0	0	1.0	2.5	2.6	–	4.1	4.0	4.2	0	0	0	0	0	–
Peroxisomes	0	0	0	0	0	–	0	0	0	0	0	0	0	0	–
SpindlePole	0	0	0	0	0	–	0	0	0	0	0	0	0	0	–
Vacuole	-2.3	0.9	-2.1	-2.0	-2.4	–	-1.1	-3.2	-2.7	3.2	3.4	5.2	0	2.2	–

External localization resources

dHITS | Loqate | YeastGFP | YeastRGB | YeastRC

Images

Protein Concentration and Protein Localization Data

	R1						R2						R3
	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Concentration	24.5511	32.3995	30.975	29.9604	30.6707	31.2572	25.7041	33.4544	30.215	32.6029	28.9145	32.6361	27.3391	36.9467	34.2823	34.4152	31.5688	34.8757
Actin	0.0304	0.0092	0.0072	0.0149	0.0007	0.0036	0.0225	0.0102	0.0481	0.0029	0.0668	0.0021	0.0768	0.0142	0.0328	0.0242	0.0266	0.0019
Bud	0.0005	0.0008	0.0009	0.0004	0.0003	0.0001	0.0007	0.0005	0.0004	0	0.0002	0	0.0003	0.007	0.0005	0.0003	0.0003	0
Bud Neck	0.0009	0.0004	0.0002	0.0006	0.0002	0.0001	0.0025	0.0003	0.0007	0.0001	0.0003	0.0001	0.0005	0.0005	0.0002	0.0002	0.0002	0.0001
Bud Periphery	0.0006	0.0004	0.0014	0.0005	0.0004	0.0001	0.0012	0.0003	0.0006	0.0001	0.0004	0	0.0005	0.0107	0.0008	0.0007	0.0004	0.0001
Bud Site	0.0011	0.006	0.0005	0.0011	0.0001	0.0001	0.0043	0.0026	0.0009	0	0.0017	0	0.001	0.004	0.0008	0.0002	0.0002	0
Cell Periphery	0.0006	0.001	0.0008	0.0007	0.0009	0.0001	0.0006	0.0005	0.0005	0.0002	0.0001	0	0.0004	0.001	0.0004	0.0006	0.0002	0.0001
Cytoplasm	0.0081	0.0049	0.0045	0.0014	0.0014	0.0019	0.0023	0.0159	0.0038	0.0003	0.0003	0.0002	0.0013	0.0015	0.0006	0.0003	0.0005	0.0001
Cytoplasmic Foci	0.0265	0.017	0.0124	0.0136	0.0089	0.0098	0.0187	0.0132	0.0109	0.0043	0.0045	0.0032	0.0307	0.0087	0.0072	0.0049	0.0056	0.0026
Eisosomes	0.0007	0.0003	0.0001	0.0004	0	0.0001	0.0004	0.0001	0.0005	0.0002	0.0002	0	0.0013	0.0004	0.0002	0.001	0.0002	0.0001
Endoplasmic Reticulum	0.004	0.0014	0.0016	0.0011	0.0008	0.001	0.0035	0.0027	0.0048	0.0007	0.0012	0.0003	0.0013	0.001	0.001	0.001	0.0011	0.0005
Endosome	0.1036	0.1543	0.1441	0.228	0.1832	0.0782	0.1107	0.1152	0.1094	0.1709	0.0923	0.0477	0.1225	0.112	0.0733	0.0986	0.1369	0.048
Golgi	0.5416	0.6598	0.6649	0.6532	0.5292	0.8129	0.6026	0.6917	0.6652	0.6303	0.7188	0.9109	0.6341	0.7571	0.6853	0.7908	0.6615	0.8529
Lipid Particles	0.0231	0.0228	0.0178	0.0112	0.0398	0.0057	0.0362	0.003	0.0096	0.0013	0.003	0.0009	0.0084	0.0025	0.0196	0.0024	0.0216	0.007
Mitochondria	0.1722	0.046	0.0669	0.0296	0.0155	0.05	0.1193	0.0962	0.0821	0.0796	0.0414	0.0184	0.0842	0.0285	0.0924	0.0172	0.1234	0.0656
None	0.0062	0.0001	0.0018	0.0001	0	0.0013	0.0047	0.0002	0.0031	0	0.0002	0	0.0011	0.0001	0.0009	0	0.0001	0
Nuclear Periphery	0.0146	0.001	0.0035	0.0005	0.0014	0.0076	0.0131	0.005	0.004	0.0006	0.0358	0.0003	0.0012	0.001	0.0023	0.0004	0.0005	0.0008
Nucleolus	0.0021	0.0037	0.0045	0.0001	0.001	0.0005	0.002	0.001	0.0002	0	0.0001	0	0.0004	0.0003	0.0002	0.0001	0	0.0001
Nucleus	0.01	0.0007	0.0023	0.0001	0.0008	0.0037	0.0036	0.0013	0.0006	0.0001	0.0008	0	0.0004	0.0006	0.0003	0.0001	0.0001	0.0001
Peroxisomes	0.0073	0.0006	0.0005	0.0032	0.0002	0.0001	0.0028	0.0005	0.0049	0	0.0012	0	0.0009	0.0002	0.0022	0.0001	0.0002	0.0001
Punctate Nuclear	0.0192	0.0001	0.0017	0.0001	0	0.0044	0.0067	0.0012	0.0009	0	0.0002	0	0.0122	0	0.0007	0	0	0
Vacuole	0.0087	0.0241	0.0179	0.0136	0.0674	0.0033	0.01	0.0109	0.0119	0.0167	0.0028	0.0041	0.0044	0.0184	0.0156	0.0163	0.0049	0.0032
Vacuole Periphery	0.018	0.0454	0.0448	0.0254	0.1479	0.0155	0.0316	0.0275	0.0368	0.0918	0.0279	0.0118	0.0161	0.0306	0.0626	0.0405	0.0155	0.0166

Sequencing Data

	R1					R2
	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Gene Expression	227.6575	202.1442	185.9266	208.585	191.4227	194.5027	203.7655	201.2286	192.248	225.7676
Translational Efficiency	0.6167	0.6744	0.6405	0.5836	0.5629	0.6537	0.675	0.5124	0.5686	0.5101

Hit Data

Dataset	Hit
Protein Concentration	✔
Protein Localization	✘
Gene Expression	✔
Translational Efficiency	✘

Proteome Dynamics

Micrographs

Cell Count

R1				R2				R1 & R2
WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
1451	1268	221	1021	1031	1721	2892	2330	2482	2989	3113	3351

Protein Abundance

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Mean	2276.66	2183.27	3499.99	3087.22	1524.63	1962.63	3008.78	3148.32	1964.27	2056.23	3043.65	3129.70
Standard Deviation	387.98	438.40	475.39	697.55	364.24	377.75	464.14	586.86	529.58	419.03	481.76	623.31
Intensity Change Log ₂	—	-0.060428	0.620432	0.439389	—	0.364329	0.980719	1.046123	—	0.125196	0.775896	0.714025

Localization Score (DeepLoc)

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Actin	0.003056	0.006582	0.015180	0.007170	0.005387	0.002139	0.001175	0.001048	0.004024	0.004024	0.002169	0.002913
Bud Neck	0.000433	0.000576	0.010669	0.001668	0.000442	0.000305	0.000163	0.000162	0.000437	0.000420	0.000909	0.000620
Bud Site	0.001039	0.001012	0.002623	0.007469	0.002387	0.000481	0.000467	0.000648	0.001599	0.000706	0.000620	0.002726
Cell Periphery	0.000375	0.000290	0.001392	0.007356	0.000483	0.000174	0.000354	0.000424	0.000420	0.000223	0.000428	0.002536
Cytoplasm	0.000110	0.000407	0.000109	0.001089	0.000693	0.000768	0.000097	0.000266	0.000352	0.000615	0.000097	0.000517
Cytoplasmic Foci	0.006576	0.018766	0.013886	0.012338	0.026902	0.031798	0.006742	0.009001	0.015019	0.026269	0.007250	0.010018
Eisosomes	0.000138	0.000273	0.000523	0.000293	0.000150	0.000076	0.000063	0.000031	0.000143	0.000160	0.000096	0.000111
Endoplasmic Reticulum	0.002330	0.002675	0.003981	0.011542	0.004954	0.001346	0.001868	0.001155	0.003420	0.001910	0.002018	0.004320
Endosome	0.009652	0.010738	0.015301	0.018685	0.017693	0.020720	0.005460	0.012742	0.012992	0.016485	0.006158	0.014553
Golgi	0.866902*	0.849722*	0.624889*	0.773378*	0.813095*	0.832772*	0.910469*	0.884713*	0.844551*	0.839962*	0.890195*	0.850791*
Lipid Particles	0.011279	0.027626	0.082097	0.038192	0.011475	0.011308	0.009192	0.006759	0.011360	0.018230	0.014368	0.016336
Mitochondria	0.007478	0.030927	0.011545	0.002297	0.069313	0.037843	0.005791	0.004078	0.033164	0.034909	0.006200	0.003535
Mitotic Spindle	0.001253	0.000205	0.002074	0.001095	0.000665	0.000880	0.000063	0.000892	0.001009	0.000593	0.000206	0.000954
None	0.000205	0.000595	0.000796	0.000239	0.000724	0.000407	0.000162	0.000089	0.000420	0.000486	0.000207	0.000135
Nuclear Periphery	0.000398	0.000407	0.006413	0.000486	0.000319	0.000531	0.000252	0.000390	0.000365	0.000478	0.000689	0.000419
Nuclear Periphery Foci	0.001099	0.000509	0.005464	0.001977	0.000447	0.001017	0.000271	0.000796	0.000828	0.000802	0.000640	0.001156
Nucleolus	0.000040	0.000064	0.000192	0.000033	0.000050	0.000159	0.000010	0.000009	0.000044	0.000119	0.000023	0.000016
Nucleus	0.000021	0.000074	0.000108	0.000052	0.000385	0.000343	0.000007	0.000009	0.000172	0.000229	0.000014	0.000022
Peroxisomes	0.000053	0.000103	0.000181	0.000210	0.000240	0.000125	0.000030	0.000025	0.000130	0.000116	0.000041	0.000081
Vacuole	0.022231	0.008460	0.072997	0.059938	0.007335	0.012466	0.006621	0.023310	0.016043	0.010767	0.011333	0.034470
Vacuole Periphery	0.065331	0.039988	0.129580*	0.054494	0.036861	0.044344	0.050742	0.053452	0.053505	0.042496	0.056339	0.053770

Localization Changes (T-score)

	R1					R2					R1 & R2
	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14
Actin	-2.69	-3.35	-2.63	-0.40	2.11	2.79	4.09	4.05	1.52	0.26	0.02	2.57	1.36	1.45	-1.26
Bud Neck	-1.25	-6.65	-4.38	-3.91	5.75	1.08	2.44	2.42	1.86	0.00	0.26	-3.37	-1.73	-2.05	1.83
Bud Site	0.08	-3.17	-5.26	-5.13	-3.95	2.65	2.67	2.44	-0.80	-0.87	2.24	2.62	-2.31	-4.75	-5.18
Cell Periphery	1.73	-6.66	-8.30	-8.40	-6.99	4.54	1.62	0.78	-5.84	-1.18	5.13	-0.11	-7.98	-8.76	-7.90
Cytoplasm	-1.06	0.22	-3.98	-1.85	-4.01	-0.25	2.56	1.74	1.20	-1.72	-0.98	2.38	-1.27	0.40	-3.92
Cytoplasmic Foci	-10.95	-4.07	-4.40	4.03	0.70	-2.69	14.85	12.70	17.45	-4.51	-10.56	10.95	6.07	16.90	-5.15
Eisosomes	-2.78	-4.36	-2.24	-0.34	2.36	3.87	6.03	8.48	3.10	4.33	-0.59	1.86	1.06	2.12	-0.80
Endoplasmic Reticulum	-0.71	-2.47	-7.37	-7.38	-5.87	5.11	4.38	5.43	1.14	4.38	3.82	3.55	-1.69	-6.10	-5.83
Endosome	-1.15	-3.92	-8.05	-6.88	-2.11	-1.94	10.21	3.69	6.57	-10.71	-3.85	10.42	-1.95	2.23	-14.40
Golgi	2.28	12.04	10.25	8.10	-6.97	-2.14	-12.13	-8.51	-8.28	5.90	0.77	-8.69	-1.00	-1.92	8.51
Lipid Particles	-6.52	-7.57	-7.74	-2.74	4.44	0.09	1.26	2.56	3.44	2.07	-4.66	-2.10	-3.29	1.10	-1.30
Mitochondria	-8.01	-1.67	4.08	10.02	3.93	5.12	11.55	11.83	11.60	1.72	-0.55	10.79	11.90	15.11	3.15
Mitotic Spindle	2.16	-0.93	0.26	-2.88	1.22	-0.48	2.30	-0.55	-0.02	-2.26	1.05	2.61	0.10	-1.00	-2.71
None	-9.72	-7.80	-1.68	8.49	7.22	2.69	9.64	10.90	3.39	7.48	-1.15	7.76	10.55	6.11	6.08
Nuclear Periphery	-0.11	-5.28	-1.43	-1.48	5.20	-2.22	1.06	-0.67	1.22	-1.54	-1.91	-3.40	-0.75	0.74	2.50
Nuclear Periphery Foci	1.91	-4.14	-2.28	-5.67	3.36	-2.34	2.30	-2.88	0.90	-5.18	0.16	1.06	-1.55	-2.02	-4.11
Nucleolus	-0.65	-5.09	0.50	0.96	6.44	-1.23	2.73	2.81	1.69	0.51	-1.39	1.88	2.49	1.93	2.31
Nucleus	-1.13	-7.03	-4.31	0.47	4.44	0.11	1.19	1.19	1.21	-1.72	-0.26	1.20	1.14	1.30	-4.21
Peroxisomes	-1.65	-3.62	-2.60	-1.69	-0.47	1.70	3.65	3.84	2.72	0.38	0.41	3.20	1.56	1.14	-1.81
Vacuole	8.24	-7.26	-9.59	-13.64	1.56	-3.82	0.69	-9.87	-6.86	-12.12	4.66	4.01	-10.59	-14.85	-14.22
Vacuole Periphery	7.30	-6.70	2.74	-4.29	7.83	-2.45	-4.81	-5.44	-3.31	-1.07	4.61	-1.09	-0.14	-5.31	1.05