Standard name | |
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Description | Alpha subunit of COPI vesicle coatomer complex; complex surrounds transport vesicles in the early secretory pathway |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 67 | 52 | 24 | 13 | 31 | 21 | 28 | 25 | – | 15 | 18 | 24 | 26 | 27 | 19 | 7 | 9 | – | 13 | 11 | 16 | 7 | 15 | 21 |
Bud | 3 | 3 | 5 | 1 | 2 | 2 | 1 | 4 | – | 4 | 7 | 10 | 2 | 2 | 0 | 0 | 0 | – | 1 | 2 | 2 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 13 | 15 | 12 | 3 | 10 | 7 | 10 | 8 | – | 6 | 5 | 7 | 9 | 9 | 26 | 2 | 4 | – | 1 | 7 | 1 | 0 | 0 | 1 |
Endoplasmic Reticulum | 16 | 32 | 12 | 4 | 4 | 8 | 8 | 8 | – | 3 | 5 | 5 | 16 | 18 | 14 | 1 | 4 | – | 1 | 3 | 2 | 0 | 1 | 2 |
Endosome | 0 | 2 | 1 | 0 | 1 | 0 | 1 | 0 | – | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | – | 5 | 11 | 7 | 8 | 3 | 3 |
Golgi | 497 | 201 | 205 | 110 | 250 | 79 | 92 | 77 | – | 64 | 38 | 66 | 199 | 196 | 194 | 36 | 86 | – | 537 | 300 | 291 | 236 | 242 | 329 |
Mitochondria | 194 | 161 | 186 | 153 | 173 | 212 | 254 | 217 | – | 265 | 245 | 363 | 24 | 24 | 10 | 3 | 13 | – | 13 | 9 | 8 | 8 | 6 | 6 |
Nucleus | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 13 | 4 | 5 | 4 | 5 | 7 | 15 | 14 | – | 27 | 23 | 35 | 2 | 2 | 3 | 0 | 5 | – | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 1 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | 77 | 40 | 27 | 6 | 34 | 10 | 13 | 9 | – | 19 | 6 | 15 | 40 | 41 | 60 | 5 | 16 | – | 17 | 46 | 4 | 11 | 7 | 3 |
Unique Cell Count | 555 | 320 | 322 | 190 | 329 | 251 | 296 | 255 | 307 | 268 | 401 | 231 | 228 | 247 | 40 | 101 | 599 | 398 | 342 | 277 | 283 | 377 | ||
Labelled Cell Count | 880 | 513 | 478 | 294 | 510 | 348 | 424 | 364 | 406 | 352 | 532 | 318 | 319 | 327 | 54 | 137 | 599 | 398 | 342 | 277 | 283 | 377 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 34.0 | 30.0 | 26.2 | 24.4 | 25.6 | 24.3 | 24.9 | 24.7 | – | 24.1 | 22.8 | 22.9 | 32.5 | 32.5 | 32.8 | 25.0 | 28.5 | – | 31.0 | 33.2 | 35.1 |
Std Deviation (1e-4) | 6.0 | 8.2 | 6.3 | 5.2 | 5.7 | 6.1 | 6.5 | 6.7 | – | 6.0 | 6.4 | 6.1 | 7.9 | 7.9 | 9.5 | 5.7 | 9.7 | – | 6.4 | 9.6 | 7.1 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.03 | -0.11 | -0.07 | -0.08 | – | -0.12 | -0.2 | -0.19 | 0.31 | 0.31 | 0.33 | -0.07 | 0.12 | – | 0.24 | 0.34 | 0.42 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.3 | 0.9 | 0.4 | 0.9 | 1.0 | – | -1.3 | -0.3 | -0.8 | 1.5 | 1.7 | 0.1 | 0 | 0.5 | – |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0.9 | 0.9 | 0 | 0 | 0 | 0 | 0 | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cytoplasm | -1.4 | -0.5 | -0.6 | -0.2 | -0.4 | – | -1.3 | -1.3 | -1.7 | 0.1 | 0.1 | 3.2 | 0 | 0 | – |
Endoplasmic Reticulum | -1.0 | -2.1 | -0.3 | -0.7 | -0.4 | – | -2.3 | -1.3 | -2.2 | 1.7 | 2.1 | 1.1 | 0 | 0 | – |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Golgi | -1.3 | 3.4 | -7.6 | -8.1 | -8.0 | – | -10.8 | -12.2 | -13.0 | 5.9 | 5.8 | 3.8 | 3.3 | 4.1 | – |
Mitochondria | 5.3 | -1.3 | 6.9 | 7.7 | 7.1 | – | 7.9 | 9.2 | 10.2 | -11.3 | -11.2 | -13.4 | -6.0 | -7.9 | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nucleolus | 0 | 0 | 1.0 | 2.5 | 2.6 | – | 4.1 | 4.0 | 4.2 | 0 | 0 | 0 | 0 | 0 | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Vacuole | -2.3 | 0.9 | -2.1 | -2.0 | -2.4 | – | -1.1 | -3.2 | -2.7 | 3.2 | 3.4 | 5.2 | 0 | 2.2 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 24.5511 | 32.3995 | 30.975 | 29.9604 | 30.6707 | 31.2572 | 25.7041 | 33.4544 | 30.215 | 32.6029 | 28.9145 | 32.6361 | 27.3391 | 36.9467 | 34.2823 | 34.4152 | 31.5688 | 34.8757 |
Actin | 0.0304 | 0.0092 | 0.0072 | 0.0149 | 0.0007 | 0.0036 | 0.0225 | 0.0102 | 0.0481 | 0.0029 | 0.0668 | 0.0021 | 0.0768 | 0.0142 | 0.0328 | 0.0242 | 0.0266 | 0.0019 |
Bud | 0.0005 | 0.0008 | 0.0009 | 0.0004 | 0.0003 | 0.0001 | 0.0007 | 0.0005 | 0.0004 | 0 | 0.0002 | 0 | 0.0003 | 0.007 | 0.0005 | 0.0003 | 0.0003 | 0 |
Bud Neck | 0.0009 | 0.0004 | 0.0002 | 0.0006 | 0.0002 | 0.0001 | 0.0025 | 0.0003 | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
Bud Periphery | 0.0006 | 0.0004 | 0.0014 | 0.0005 | 0.0004 | 0.0001 | 0.0012 | 0.0003 | 0.0006 | 0.0001 | 0.0004 | 0 | 0.0005 | 0.0107 | 0.0008 | 0.0007 | 0.0004 | 0.0001 |
Bud Site | 0.0011 | 0.006 | 0.0005 | 0.0011 | 0.0001 | 0.0001 | 0.0043 | 0.0026 | 0.0009 | 0 | 0.0017 | 0 | 0.001 | 0.004 | 0.0008 | 0.0002 | 0.0002 | 0 |
Cell Periphery | 0.0006 | 0.001 | 0.0008 | 0.0007 | 0.0009 | 0.0001 | 0.0006 | 0.0005 | 0.0005 | 0.0002 | 0.0001 | 0 | 0.0004 | 0.001 | 0.0004 | 0.0006 | 0.0002 | 0.0001 |
Cytoplasm | 0.0081 | 0.0049 | 0.0045 | 0.0014 | 0.0014 | 0.0019 | 0.0023 | 0.0159 | 0.0038 | 0.0003 | 0.0003 | 0.0002 | 0.0013 | 0.0015 | 0.0006 | 0.0003 | 0.0005 | 0.0001 |
Cytoplasmic Foci | 0.0265 | 0.017 | 0.0124 | 0.0136 | 0.0089 | 0.0098 | 0.0187 | 0.0132 | 0.0109 | 0.0043 | 0.0045 | 0.0032 | 0.0307 | 0.0087 | 0.0072 | 0.0049 | 0.0056 | 0.0026 |
Eisosomes | 0.0007 | 0.0003 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0 | 0.0013 | 0.0004 | 0.0002 | 0.001 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.004 | 0.0014 | 0.0016 | 0.0011 | 0.0008 | 0.001 | 0.0035 | 0.0027 | 0.0048 | 0.0007 | 0.0012 | 0.0003 | 0.0013 | 0.001 | 0.001 | 0.001 | 0.0011 | 0.0005 |
Endosome | 0.1036 | 0.1543 | 0.1441 | 0.228 | 0.1832 | 0.0782 | 0.1107 | 0.1152 | 0.1094 | 0.1709 | 0.0923 | 0.0477 | 0.1225 | 0.112 | 0.0733 | 0.0986 | 0.1369 | 0.048 |
Golgi | 0.5416 | 0.6598 | 0.6649 | 0.6532 | 0.5292 | 0.8129 | 0.6026 | 0.6917 | 0.6652 | 0.6303 | 0.7188 | 0.9109 | 0.6341 | 0.7571 | 0.6853 | 0.7908 | 0.6615 | 0.8529 |
Lipid Particles | 0.0231 | 0.0228 | 0.0178 | 0.0112 | 0.0398 | 0.0057 | 0.0362 | 0.003 | 0.0096 | 0.0013 | 0.003 | 0.0009 | 0.0084 | 0.0025 | 0.0196 | 0.0024 | 0.0216 | 0.007 |
Mitochondria | 0.1722 | 0.046 | 0.0669 | 0.0296 | 0.0155 | 0.05 | 0.1193 | 0.0962 | 0.0821 | 0.0796 | 0.0414 | 0.0184 | 0.0842 | 0.0285 | 0.0924 | 0.0172 | 0.1234 | 0.0656 |
None | 0.0062 | 0.0001 | 0.0018 | 0.0001 | 0 | 0.0013 | 0.0047 | 0.0002 | 0.0031 | 0 | 0.0002 | 0 | 0.0011 | 0.0001 | 0.0009 | 0 | 0.0001 | 0 |
Nuclear Periphery | 0.0146 | 0.001 | 0.0035 | 0.0005 | 0.0014 | 0.0076 | 0.0131 | 0.005 | 0.004 | 0.0006 | 0.0358 | 0.0003 | 0.0012 | 0.001 | 0.0023 | 0.0004 | 0.0005 | 0.0008 |
Nucleolus | 0.0021 | 0.0037 | 0.0045 | 0.0001 | 0.001 | 0.0005 | 0.002 | 0.001 | 0.0002 | 0 | 0.0001 | 0 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0001 |
Nucleus | 0.01 | 0.0007 | 0.0023 | 0.0001 | 0.0008 | 0.0037 | 0.0036 | 0.0013 | 0.0006 | 0.0001 | 0.0008 | 0 | 0.0004 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Peroxisomes | 0.0073 | 0.0006 | 0.0005 | 0.0032 | 0.0002 | 0.0001 | 0.0028 | 0.0005 | 0.0049 | 0 | 0.0012 | 0 | 0.0009 | 0.0002 | 0.0022 | 0.0001 | 0.0002 | 0.0001 |
Punctate Nuclear | 0.0192 | 0.0001 | 0.0017 | 0.0001 | 0 | 0.0044 | 0.0067 | 0.0012 | 0.0009 | 0 | 0.0002 | 0 | 0.0122 | 0 | 0.0007 | 0 | 0 | 0 |
Vacuole | 0.0087 | 0.0241 | 0.0179 | 0.0136 | 0.0674 | 0.0033 | 0.01 | 0.0109 | 0.0119 | 0.0167 | 0.0028 | 0.0041 | 0.0044 | 0.0184 | 0.0156 | 0.0163 | 0.0049 | 0.0032 |
Vacuole Periphery | 0.018 | 0.0454 | 0.0448 | 0.0254 | 0.1479 | 0.0155 | 0.0316 | 0.0275 | 0.0368 | 0.0918 | 0.0279 | 0.0118 | 0.0161 | 0.0306 | 0.0626 | 0.0405 | 0.0155 | 0.0166 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 227.6575 | 202.1442 | 185.9266 | 208.585 | 191.4227 | 194.5027 | 203.7655 | 201.2286 | 192.248 | 225.7676 |
Translational Efficiency | 0.6167 | 0.6744 | 0.6405 | 0.5836 | 0.5629 | 0.6537 | 0.675 | 0.5124 | 0.5686 | 0.5101 |
Dataset | Hit |
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Protein Abundance | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |