Standard name
Human Ortholog
Description RNA polymerase II largest subunit B220; part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0 0 0 0 0 0.08 0 0 0 0.06 0.08 0.09 0.13 0.09 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.14 0.14 0.12 0.12 0 0 0 0.07 0 0.09 0 0 0 0 0 0
Nucleus 0.79 0.79 0.76 0.87 0.81 0.58 0.6 0.47 0.41 0.48 0.48 0.45 0.75 0.72 0.69 0.61 0.7 0.7 0.68 0.66 0.51 0.61 0.55 0.43
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.48 0.66 0.54 0.38 0.25 0.42 0.37 0.49 0.55 0.56 0.59 0.62 0.58 0.52 0.47 0.34 0.31 0.36 0.28 0.25 0.39 0.3 0.34 0.42
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.07 0.09 0.08 0 0 0 0.05 0 0 0 0.06 0 0 0 0 0 0.05 0.09 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 1 1 1 0 0 0 0 0 3 0 0 0
Bud 0 0 0 0 0 1 1 0 1 0 2 1 0 0 0 0 0 0 1 0 1 2 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 0 5 1 3 0 2 0 1 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 16 1 8 8 3 9 13 14 15 8 7 7 9 16 17 25 18 20 0 1 0 1 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 2 1 3 0 0 1 0 0 0
Endosome 1 0 0 0 0 0 0 1 0 1 0 0 2 3 2 3 0 0 0 0 1 0 1 3
Golgi 0 0 0 0 4 3 4 10 2 3 1 1 0 0 0 1 7 1 0 0 1 0 0 0
Mitochondria 3 5 5 5 1 6 7 19 27 43 32 32 1 2 5 13 7 17 0 1 1 0 0 1
Nucleus 278 121 185 203 233 118 185 230 81 151 123 119 121 141 131 115 145 136 242 110 135 91 133 88
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 1 0 0 0 1
Nucleolus 168 101 131 88 73 85 115 241 110 175 153 163 94 101 89 64 63 69 100 41 103 44 82 86
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 1 0 0 0 1 0 0 1
Vac/Vac Membrane 0 1 1 1 12 14 28 38 7 11 9 12 0 2 2 12 3 6 8 6 10 7 21 16
Unique Cell Count 350 153 244 234 287 202 310 489 199 315 258 265 162 195 190 187 206 194 357 167 265 149 242 204
Labelled Cell Count 467 229 330 305 326 237 354 560 244 396 329 338 230 270 247 236 245 252 357 167 265 149 242 204


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 42.0 28.4 21.9 21.7 28.3 29.8 30.1 30.2 28.0 27.0 26.2 24.4 35.6 33.1 28.6 33.2 38.6 37.2 31.1 32.0 34.6
Std Deviation (1e-4) 16.5 11.8 8.0 6.8 7.1 8.7 8.7 8.9 10.4 8.2 9.0 8.5 10.9 12.1 10.4 18.1 15.8 14.9 8.7 10.2 13.8
Intensity Change (Log2) – – – -0.01 0.37 0.45 0.46 0.47 0.36 0.3 0.26 0.16 0.7 0.6 0.39 0.6 0.82 0.77 0.51 0.55 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 -1.8 0.6 0.6 -0.3 2.0 -0.5 -0.4 -0.4 1.1 2.3 2.5 3.9 2.5 3.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.2 1.3 4.7 4.9 4.4 4.4 0 0 0 2.5 0.9 3.2
Nucleus 3.1 1.5 -3.9 -4.0 -7.4 -7.5 -6.7 -6.5 -7.1 -0.3 -0.8 -1.6 -3.2 -1.3 -1.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -3.5 -6.7 -2.4 -3.9 -1.1 0.3 0.4 1.3 1.8 0.9 -0.4 -1.4 -4.0 -4.9 -3.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.8 3.8 4.5 4.2 0 2.5 0 2.9 0 0 0 3.6 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 27.9728 31.0872 30.3171 29.0182 22.6527 31.427 29.8087 37.3916 33.156 30.6333 32.632 34.5283 36.3483 40.174 34.8596 37.9677 33.3852 36.121
Actin 0.0049 0.0006 0.0075 0 0.0117 0.0112 0.0302 0 0.0009 0 0.0419 0.0025 0.0044 0 0.0054 0 0.0002 0
Bud 0.0003 0.0001 0.0003 0 0.0007 0.0012 0.0005 0 0 0.0001 0.0011 0.0001 0.0001 0 0.0001 0 0.0001 0
Bud Neck 0.0003 0.0002 0.0001 0 0.0097 0.0008 0.0004 0 0 0 0.0004 0.0002 0 0 0.0001 0 0.0001 0.0002
Bud Periphery 0.0003 0.0001 0.0004 0 0.0014 0.0034 0.0008 0 0 0.0001 0.002 0.0001 0.0001 0 0.0001 0 0.0001 0
Bud Site 0.0008 0.0015 0.0008 0 0.0008 0.0018 0.0012 0 0.0001 0 0.0021 0.0001 0.0001 0 0.0003 0 0.0001 0
Cell Periphery 0.0003 0 0.0002 0 0.0008 0.0007 0.0001 0 0 0.0001 0.0003 0.0001 0.0001 0 0.0001 0 0.0001 0.0001
Cytoplasm 0.0019 0.0002 0.0008 0 0.0005 0.0002 0.0012 0 0.0005 0 0.0002 0.0001 0.0001 0 0.0006 0 0.0017 0
Cytoplasmic Foci 0.0025 0.0003 0.0016 0 0.0202 0.0004 0.0139 0 0.0006 0 0.0003 0.0057 0.0009 0 0.0048 0 0.0419 0.0013
Eisosomes 0.0001 0 0.0001 0 0.0002 0.0001 0.0001 0 0 0 0.0004 0 0 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0019 0.0001 0.0012 0 0.0022 0.0003 0.0014 0 0.0003 0 0.0006 0.0001 0.0002 0 0.0004 0 0.0005 0
Endosome 0.012 0.0006 0.0084 0 0.0259 0.0014 0.0119 0 0.0039 0 0.0014 0.0012 0.0018 0 0.0017 0 0.0076 0
Golgi 0.0019 0.0002 0.0014 0 0.0151 0.0009 0.0071 0 0.0028 0 0.0014 0.0014 0.0016 0 0.0011 0 0.0007 0
Lipid Particles 0.0031 0.0001 0.0027 0 0.0493 0.0003 0.01 0 0.0008 0 0.0005 0.0048 0.0017 0 0.0035 0 0.0119 0.0006
Mitochondria 0.0038 0.0004 0.0049 0.0004 0.0648 0.0124 0.0071 0.0002 0.001 0.0007 0.0201 0.0031 0.0017 0.0002 0.0028 0.0001 0.0078 0.0011
None 0.0018 0.0001 0.003 0 0.0011 0.0003 0.0014 0 0.0002 0 0.0004 0.0002 0.0002 0 0.0016 0 0.0035 0.0001
Nuclear Periphery 0.0135 0.0011 0.0047 0.0001 0.0115 0.0027 0.0259 0.0001 0.0062 0.0003 0.003 0.0003 0.0013 0.0001 0.0007 0.0001 0.001 0
Nucleolus 0.1945 0.0862 0.0744 0.0416 0.3797 0.2585 0.0804 0.0525 0.0569 0.1114 0.3481 0.209 0.1062 0.075 0.0834 0.0382 0.4 0.2433
Nucleus 0.7478 0.9031 0.8829 0.9577 0.3872 0.6977 0.7798 0.9471 0.9244 0.8869 0.5725 0.7684 0.8775 0.9246 0.888 0.9616 0.5144 0.7526
Peroxisomes 0.0008 0.0001 0.0007 0 0.008 0.0006 0.0098 0 0.0001 0 0.0005 0.0009 0.0008 0 0.0031 0 0.0008 0.0001
Punctate Nuclear 0.0052 0.0047 0.0026 0 0.0042 0.0012 0.0147 0 0.0006 0.0001 0.0016 0.0013 0.0008 0 0.001 0 0.0061 0.0001
Vacuole 0.0012 0.0001 0.0006 0 0.0019 0.0027 0.0007 0 0.0002 0.0001 0.0006 0.0002 0.0002 0 0.0004 0 0.0004 0.0001
Vacuole Periphery 0.001 0.0001 0.0008 0 0.003 0.0012 0.0014 0 0.0004 0.0002 0.0008 0.0003 0.0003 0 0.0007 0 0.0009 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 172.977 190.7525 181.7875 209.0006 155.7943 150.7278 207.1048 190.7321 184.464 192.7562
Translational Efficiency 1.0367 0.9333 0.8554 0.675 0.746 1.0092 0.7715 0.5756 0.657 0.6364

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
53 135 860 1545 1806 1719 1598 166 1859 1854 2458 1711

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2395.91 2379.85 3823.00 3745.32 2151.80 2118.77 3378.61 3760.87 2158.76 2137.78 3534.09 3746.83
Standard Deviation 794.85 677.68 994.76 906.13 493.35 538.63 682.32 856.86 506.08 554.11 832.95 901.48
Intensity Change Log 2 — -0.009703 0.674131 0.644515 — -0.022317 0.650886 0.805522 — -0.015658 0.663179 0.722941

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000599 0.000743 0.000694 0.000712 0.000510 0.000633 0.000675 0.000587 0.000513 0.000641 0.000682 0.000700
Bud Neck 0.003451 0.002570 0.010063 0.022806 0.002664 0.006025 0.011653 0.011767 0.002686 0.005773 0.011096 0.021735
Bud Site 0.000658 0.000485 0.001118 0.003032 0.000366 0.001376 0.001114 0.001964 0.000375 0.001311 0.001115 0.002928
Cell Periphery 0.000107 0.000021 0.000040 0.000217 0.000020 0.000181 0.000034 0.000078 0.000022 0.000170 0.000036 0.000203
Cytoplasm 0.000735 0.001536 0.000420 0.001702 0.000074 0.001026 0.000084 0.001883 0.000093 0.001063 0.000201 0.001720
Cytoplasmic Foci 0.000619 0.000565 0.001135 0.002903 0.001897 0.001726 0.000629 0.000635 0.001861 0.001641 0.000806 0.002683
Eisosomes 0.000090 0.000134 0.000155 0.000079 0.000258 0.000237 0.000147 0.000101 0.000253 0.000229 0.000150 0.000081
Endoplasmic Reticulum 0.000414 0.000404 0.000197 0.000396 0.000157 0.000367 0.000212 0.000196 0.000164 0.000369 0.000207 0.000377
Endosome 0.000108 0.000037 0.000008 0.001255 0.000023 0.000419 0.000013 0.000011 0.000025 0.000391 0.000011 0.001134
Golgi 0.000153 0.000272 0.000081 0.000674 0.000054 0.000284 0.000073 0.000092 0.000057 0.000283 0.000076 0.000618
Lipid Particles 0.000090 0.000047 0.000006 0.000193 0.000016 0.000260 0.000005 0.000008 0.000018 0.000244 0.000005 0.000175
Mitochondria 0.001679 0.000967 0.001833 0.002454 0.000851 0.001458 0.001618 0.001634 0.000875 0.001423 0.001693 0.002374
Mitotic Spindle 0.000060 0.002264 0.000187 0.011592 0.000145 0.004278 0.001127 0.001157 0.000143 0.004131 0.000798 0.010580
None 0.001263 0.003249 0.000773 0.002306 0.000393 0.001532 0.000605 0.002611 0.000418 0.001657 0.000664 0.002336
Nuclear Periphery 0.000375 0.000016 0.000061 0.000371 0.000014 0.000143 0.000045 0.000167 0.000025 0.000134 0.000051 0.000351
Nuclear Periphery Foci 0.000348 0.000093 0.000005 0.000320 0.000073 0.000244 0.000015 0.000016 0.000080 0.000233 0.000012 0.000290
Nucleolus 0.059947 0.084672 0.077767 0.031391 0.084285 0.099014 0.087082 0.032736 0.083591 0.097970 0.083823 0.031521
Nucleus 0.916308* 0.889641* 0.891222* 0.913896* 0.900803* 0.871569* 0.887006* 0.940399* 0.901245* 0.872885* 0.888481* 0.916468*
Peroxisomes 0.007913 0.011764 0.013781 0.001668 0.006779 0.007838 0.007444 0.003412 0.006811 0.008124 0.009661 0.001837
Vacuole 0.001130 0.000293 0.000242 0.001252 0.000213 0.000824 0.000176 0.000393 0.000239 0.000785 0.000199 0.001169
Vacuole Periphery 0.003953 0.000230 0.000213 0.000780 0.000403 0.000565 0.000244 0.000153 0.000505 0.000540 0.000233 0.000720

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.38 -1.85 -3.05 -0.32 -2.96 -4.73 -10.97 -3.16 -1.32 -0.12 -3.81 -12.68 -12.66 -4.97 -4.05
Bud Neck 1.32 -11.13 -21.91 -23.55 -15.83 -7.38 -22.51 -7.28 -5.49 -2.29 -7.26 -27.17 -25.56 -20.90 -15.45
Bud Site 0.81 -1.73 -6.79 -8.79 -7.68 -4.41 -11.59 -2.84 -1.78 -1.97 -4.37 -13.78 -11.04 -6.16 -7.94
Cell Periphery 1.13 0.82 -1.36 -7.21 -6.62 -3.18 -6.27 -4.20 0.97 -3.41 -3.13 -5.42 -7.97 -0.47 -7.13
Cytoplasm -0.25 1.07 -1.64 -0.53 -5.39 -2.12 -1.38 -2.35 -1.69 -2.30 -2.22 -1.45 -7.77 -2.29 -7.13
Cytoplasmic Foci 0.72 -1.24 -1.92 -2.85 -1.33 0.08 1.48 0.90 1.17 -1.16 0.16 1.20 -0.07 -0.32 -2.25
Eisosomes -1.66 -4.36 0.69 2.82 10.83 0.80 8.60 11.58 5.49 6.26 0.97 8.08 15.19 6.47 16.65
Endoplasmic Reticulum 0.28 1.49 0.18 -0.22 -9.45 -4.62 -8.46 -4.40 0.91 -2.36 -4.18 -6.49 -12.17 -1.78 -10.02
Endosome 1.01 1.12 -0.85 -1.91 -2.01 -1.95 1.02 0.46 2.02 -2.22 -1.90 1.36 -1.87 -0.49 -2.01
Golgi 0.22 0.89 -0.40 -0.73 -2.66 -1.53 -0.79 -4.04 0.47 -4.48 -1.56 -0.74 -2.94 -0.93 -2.84
Lipid Particles 0.95 1.40 0.29 -0.91 -1.67 -1.51 1.19 0.60 1.61 -2.30 -1.48 1.37 -1.16 0.86 -1.70
Mitochondria 1.92 -0.80 -3.19 -12.36 -8.21 -5.12 -15.88 -7.86 -3.71 -2.49 -4.92 -19.30 -22.93 -10.46 -10.86
Mitotic Spindle -0.93 -0.74 -5.59 -2.92 -5.54 -3.36 -1.29 -1.51 -0.37 -1.24 -3.46 -1.26 -5.71 -2.95 -5.24
None -0.67 1.26 -2.77 -0.00 -7.93 -3.28 -9.44 -3.28 -2.53 -3.10 -3.33 -10.16 -9.71 -3.72 -8.69
Nuclear Periphery 1.12 0.93 -0.66 -11.09 -9.94 -4.58 -2.70 -3.25 -2.16 -2.66 -4.01 -2.92 -11.25 -7.72 -9.62
Nuclear Periphery Foci 1.24 1.48 0.97 -1.15 -3.47 -1.38 1.50 1.26 2.98 -0.73 -1.16 1.84 -0.72 0.54 -3.30
Nucleolus -1.17 -1.13 2.32 4.44 9.57 -3.22 -0.90 5.92 7.65 6.27 -3.23 -0.50 13.07 15.30 14.35
Nucleus 0.86 1.37 0.54 -0.67 -2.37 5.30 3.42 -1.07 -3.78 -2.73 5.30 3.87 0.18 -5.00 -3.54
Peroxisomes -1.11 -2.89 2.65 3.99 9.56 -1.01 -1.16 3.90 4.94 4.93 -1.35 -4.24 8.34 10.91 13.17
Vacuole 1.35 1.35 0.13 -2.72 -3.07 -2.64 -1.20 -3.45 -0.25 -3.05 -2.49 -1.03 -3.53 -1.24 -3.35
Vacuole Periphery 1.11 1.12 1.03 -1.45 -1.57 -2.25 3.63 5.20 5.36 1.90 -0.41 2.56 0.17 0.47 -1.45
Standard name
Human Ortholog
Description RNA polymerase II largest subunit B220; part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
Localization
Cell Percentages nucleus (91%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Rpo21

Rpo21


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpo21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available