Standard name
Description RNA polymerase II largest subunit B220; part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0 0 0 0 0 0.08 0 0 0 0.06 0.08 0.09 0.13 0.09 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.14 0.14 0.12 0.12 0 0 0 0.07 0 0.09 0 0 0 0 0 0
Nucleus 0.79 0.79 0.76 0.87 0.81 0.58 0.6 0.47 0.41 0.48 0.48 0.45 0.75 0.72 0.69 0.61 0.7 0.7 0.68 0.66 0.51 0.61 0.55 0.43
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.48 0.66 0.54 0.38 0.25 0.42 0.37 0.49 0.55 0.56 0.59 0.62 0.58 0.52 0.47 0.34 0.31 0.36 0.28 0.25 0.39 0.3 0.34 0.42
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.07 0.09 0.08 0 0 0 0.05 0 0 0 0.06 0 0 0 0 0 0.05 0.09 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 1 1 1 0 0 0 0 0 3 0 0 0
Bud 0 0 0 0 0 1 1 0 1 0 2 1 0 0 0 0 0 0 1 0 1 2 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 0 5 1 3 0 2 0 1 0 0 0 0
Cell Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 16 1 8 8 3 9 13 14 15 8 7 7 9 16 17 25 18 20 0 1 0 1 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 2 1 3 0 0 1 0 0 0
Endosome 1 0 0 0 0 0 0 1 0 1 0 0 2 3 2 3 0 0 0 0 1 0 1 3
Golgi 0 0 0 0 4 3 4 10 2 3 1 1 0 0 0 1 7 1 0 0 1 0 0 0
Mitochondria 3 5 5 5 1 6 7 19 27 43 32 32 1 2 5 13 7 17 0 1 1 0 0 1
Nucleus 278 121 185 203 233 118 185 230 81 151 123 119 121 141 131 115 145 136 242 110 135 91 133 88
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 1 0 0 0 1
Nucleolus 168 101 131 88 73 85 115 241 110 175 153 163 94 101 89 64 63 69 100 41 103 44 82 86
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 1 0 0 0 1 0 0 1
Vac/Vac Membrane 0 1 1 1 12 14 28 38 7 11 9 12 0 2 2 12 3 6 8 6 10 7 21 16
Unique Cell Count 350 153 244 234 287 202 310 489 199 315 258 265 162 195 190 187 206 194 357 167 265 149 242 204
Labelled Cell Count 467 229 330 305 326 237 354 560 244 396 329 338 230 270 247 236 245 252 357 167 265 149 242 204


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 42.0 28.4 21.9 21.7 28.3 29.8 30.1 30.2 28.0 27.0 26.2 24.4 35.6 33.1 28.6 33.2 38.6 37.2 31.1 32.0 34.6
Std Deviation (1e-4) 16.5 11.8 8.0 6.8 7.1 8.7 8.7 8.9 10.4 8.2 9.0 8.5 10.9 12.1 10.4 18.1 15.8 14.9 8.7 10.2 13.8
Intensity Change (Log2) -0.01 0.37 0.45 0.46 0.47 0.36 0.3 0.26 0.16 0.7 0.6 0.39 0.6 0.82 0.77 0.51 0.55 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 -1.8 0.6 0.6 -0.3 2.0 -0.5 -0.4 -0.4 1.1 2.3 2.5 3.9 2.5 3.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.2 1.3 4.7 4.9 4.4 4.4 0 0 0 2.5 0.9 3.2
Nucleus 3.1 1.5 -3.9 -4.0 -7.4 -7.5 -6.7 -6.5 -7.1 -0.3 -0.8 -1.6 -3.2 -1.3 -1.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -3.5 -6.7 -2.4 -3.9 -1.1 0.3 0.4 1.3 1.8 0.9 -0.4 -1.4 -4.0 -4.9 -3.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.8 3.8 4.5 4.2 0 2.5 0 2.9 0 0 0 3.6 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpo21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available