Standard name
Description ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication; arf1 arf2 double null mutation is complemented by human ARF1, ARF4, ARF5, or ARF6
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.06 0.05 0 0 0 0 0 0 0 0 0.05 0.14 0.1 0 0.12 0.12 0.11 0.06 0.08 0.06 0.05 0.05 0.08
Bud 0 0 0 0.05 0.08 0.06 0.07 0.05 0.08 0.06 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.47 0.7 0.42 0.3 0.25 0.23 0.16 0.16 0.15 0.15 0.19 0.19 0.42 0.26 0.42 0.38 0.49 0.41 0.19 0.27 0.16 0.05 0 0
Endoplasmic Reticulum 0.07 0.12 0 0 0 0 0 0 0 0 0.05 0.05 0.08 0.24 0.09 0.07 0 0 0 0 0.06 0.05 0 0.05
Endosome 0.25 0 0.14 0.33 0.34 0.09 0.07 0.09 0.12 0.16 0.07 0.09 0.17 0.26 0.22 0.19 0.1 0.15 0.28 0.2 0.3 0.21 0.26 0.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.14 0.17 0.13 0.18 0.17 0.09 0.15 0.2 0.23 0.31
Mitochondria 0.13 0.07 0.35 0.45 0.45 0.67 0.72 0.66 0.66 0.62 0.68 0.63 0.07 0.07 0.07 0.11 0.08 0.1 0.14 0.13 0.15 0.2 0.18 0.16
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.06 0.07 0.07 0.07 0 0.05 0.07 0.07 0.09 0 0.05 0.15 0.13 0.15 0.16 0.13 0.12 0 0.08 0 0.14 0.13 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 10 6 6 4 9 8 10 6 7 9 16 10 12 3 15 11 12 9 18 8 4 6 10
Bud 0 4 3 12 25 15 25 28 25 25 10 23 2 2 0 2 1 3 1 7 1 3 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 4 0 1 0 0
Bud Site 0 0 0 0 0 2 0 4 3 7 6 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 1 1 2 2 5 1 3 2 3 1 2 1 1 3 1 0 0 0 0 0 0
Cytoplasm 52 114 49 67 78 62 56 92 47 60 52 65 30 32 38 46 44 45 28 62 24 4 5 6
Endoplasmic Reticulum 8 20 4 4 8 8 8 17 14 6 13 16 6 29 8 9 4 3 4 7 9 5 2 6
Endosome 28 7 16 74 107 24 24 54 38 63 19 29 12 32 20 23 9 16 42 46 44 20 36 26
Golgi 3 1 1 3 8 1 0 3 0 3 2 2 4 5 13 21 12 20 26 21 22 19 31 42
Mitochondria 14 12 41 102 141 177 252 380 214 246 191 215 5 9 6 13 7 11 20 30 21 19 24 22
Nucleus 1 0 1 0 3 1 3 4 2 2 3 0 1 0 1 3 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 2 1 4 1 1 0 0 0 0 2 0 0 0 0 1 0 0
Nucleolus 0 0 0 0 3 5 9 23 9 9 9 10 0 0 0 0 2 2 0 0 0 0 0 0
Peroxisomes 0 0 1 3 8 1 4 6 4 5 1 4 1 2 0 0 0 0 1 0 1 0 1 2
SpindlePole 2 1 2 4 7 8 8 14 11 23 9 14 2 5 1 2 2 6 3 5 4 1 4 1
Vac/Vac Membrane 7 9 8 15 21 10 18 41 24 36 10 17 11 16 14 19 12 13 6 19 4 13 18 13
Unique Cell Count 110 163 116 227 311 264 350 573 322 398 281 339 72 123 91 120 90 109 153 229 148 97 138 138
Labelled Cell Count 122 179 132 291 414 325 418 683 399 499 337 418 85 146 105 154 110 133 153 229 148 97 138 138


Early Golgi, Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 7.5 6.1 6.1 6.7 6.3 5.8 6.0 6.2 6.2 6.6 6.8 8.4 8.1 8.5 11.3 11.9 11.8 9.5 9.7 9.2
Std Deviation (1e-4) 1.1 1.1 1.7 1.5 1.7 1.6 1.3 1.5 1.5 1.5 1.7 1.5 1.1 1.2 1.2 2.8 3.6 3.3 1.6 2.2 1.4
Intensity Change (Log2) – – – 0.01 0.14 0.06 -0.07 -0.02 0.04 0.02 0.12 0.16 0.46 0.41 0.49 0.9 0.97 0.96 0.65 0.68 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 -0.2 2.1 1.3 0 2.0 1.8 1.6
Bud 1.2 2.0 1.3 1.8 1.1 2.0 1.5 0 1.7 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.4 -3.5 -3.7 -5.9 -6.4 -6.2 -6.3 -4.9 -4.9 -0.1 -2.6 -0.1 -0.6 1.0 -0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.6 0 4.5 1.6 1.4 0 0
Endosome 3.7 4.2 -1.4 -2.3 -1.4 -0.6 0.5 -2.2 -1.6 0.5 2.4 1.5 1.1 -0.8 0.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 3.8 4.4 3.7 4.5
Mitochondria 1.7 1.9 5.8 7.1 6.2 5.8 5.1 6.0 5.3 -4.4 -5.3 -4.9 -4.5 -4.6 -4.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1.8 0 0 0 0 0 0 0 0
Vacuole -0.1 -0.1 -1.3 -0.7 0.1 0.2 0.7 -1.5 -0.8 1.9 1.6 2.0 2.2 1.5 1.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.9691 9.7564 9.2869 8.9575 8.2502 9.4431 8.427 9.8553 8.8776 7.691 8.0003 9.4664 7.3346 9.6114 8.4009 8.1083 7.2263 9.1881
Actin 0.0094 0.0075 0.0093 0.0017 0.0007 0.0068 0.0975 0.019 0.0345 0.121 0.0035 0.0275 0.0302 0.0431 0.0522 0.0027 0.0149 0.0335
Bud 0.0004 0.0004 0.0002 0.0002 0.0001 0.0002 0.001 0.0009 0.0003 0.0011 0.0002 0.0002 0.0009 0.0009 0.0018 0.0001 0.0018 0.0006
Bud Neck 0.0005 0.0006 0.0004 0.0003 0.0003 0.0012 0.0009 0.001 0.0008 0.001 0.0011 0.0012 0.0009 0.001 0.0014 0.0003 0.0012 0.0007
Bud Periphery 0.0003 0.0001 0.0001 0.0001 0 0.0001 0.0007 0.0004 0.0002 0.001 0.0002 0.0001 0.0007 0.0003 0.0009 0.0001 0.0026 0.0016
Bud Site 0.0013 0.0017 0.001 0.0003 0.0002 0.0003 0.0038 0.0128 0.0007 0.0027 0.0007 0.0004 0.0017 0.0035 0.0044 0.0001 0.0036 0.0004
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0002 0.0001 0 0.0002 0.0004 0.0001 0.0002 0.0001 0.0002 0 0.0004 0.0001
Cytoplasm 0.0201 0.0449 0.0115 0.0111 0.0074 0.0083 0.0217 0.0163 0.0054 0.006 0.0166 0.0057 0.0338 0.0125 0.0144 0.0022 0.0162 0.0032
Cytoplasmic Foci 0.1183 0.1883 0.1264 0.0893 0.1221 0.1027 0.13 0.1944 0.1242 0.093 0.1097 0.12 0.1288 0.16 0.0863 0.0601 0.1483 0.0627
Eisosomes 0.0001 0 0 0 0 0 0.0003 0 0 0.0009 0.0017 0 0.0001 0 0.0001 0 0.0002 0.0002
Endoplasmic Reticulum 0.0032 0.0041 0.0045 0.0018 0.0011 0.0021 0.0069 0.0011 0.001 0.0012 0.0071 0.0014 0.0044 0.001 0.0085 0.0017 0.0209 0.0021
Endosome 0.3028 0.3055 0.2925 0.4029 0.3947 0.2485 0.2833 0.2863 0.2503 0.2608 0.382 0.2477 0.2781 0.265 0.2321 0.3337 0.4446 0.215
Golgi 0.4946 0.4182 0.5138 0.4747 0.4627 0.6086 0.396 0.4516 0.567 0.4664 0.3901 0.5662 0.4369 0.4552 0.5507 0.5835 0.2075 0.6642
Lipid Particles 0.0078 0.0081 0.005 0.0028 0.0032 0.0026 0.0169 0.0021 0.003 0.006 0.0117 0.0051 0.0195 0.0037 0.0138 0.0029 0.0186 0.0017
Mitochondria 0.0178 0.005 0.0124 0.0042 0.0029 0.0087 0.009 0.0042 0.0045 0.0271 0.0102 0.0073 0.0193 0.0039 0.0191 0.0029 0.0047 0.0043
None 0.0055 0.0002 0.0002 0.0001 0.0001 0.0001 0.0032 0.0001 0 0.0002 0.0391 0.0001 0.0022 0.0001 0.0015 0 0.0102 0.0003
Nuclear Periphery 0.0045 0.0008 0.0082 0.0002 0.0001 0.0005 0.0059 0.0002 0.0001 0.0002 0.0057 0.0002 0.0181 0.0003 0.0016 0.0004 0.0483 0.0049
Nucleolus 0.0002 0 0.0001 0 0 0 0.0002 0.0001 0 0.0001 0.0023 0 0.0006 0.0001 0.0001 0 0.0017 0.0001
Nucleus 0.0033 0.0003 0.0023 0.0001 0 0.0002 0.0027 0.0002 0 0.0001 0.002 0.0001 0.0048 0.0003 0.0003 0.0001 0.0191 0.0005
Peroxisomes 0.0044 0.008 0.0061 0.0027 0.0015 0.0059 0.0121 0.0053 0.006 0.0075 0.0043 0.0135 0.006 0.0159 0.0044 0.0009 0.0104 0.001
Punctate Nuclear 0.0007 0.0004 0.0023 0.0001 0.0001 0.0002 0.0031 0.0003 0.0001 0.0002 0.0047 0.0001 0.0042 0.0003 0.0007 0.0001 0.007 0.0005
Vacuole 0.0028 0.0044 0.0018 0.0043 0.0013 0.0014 0.0028 0.0027 0.0008 0.0017 0.0036 0.0015 0.0052 0.0277 0.003 0.0041 0.0106 0.0008
Vacuole Periphery 0.0021 0.0017 0.0018 0.003 0.0015 0.0017 0.0017 0.0009 0.001 0.0017 0.003 0.0014 0.0035 0.0051 0.0027 0.004 0.0072 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 232.402 211.3663 281.513 257.5413 193.8925 272.6687 330.1581 365.5348 389.6565 309.8582
Translational Efficiency 1.4586 1.4935 1.2278 1.4678 1.661 1.3891 1.4917 1.3467 1.3646 1.3601

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘