Standard name
Description Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0 0.06 0.06 0.08 0.11 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.98 0.98 0.92 0.87 0.79 0.73 0.83 0.72 0.7 0.66 0.98 0.99 0.97 0.95 0.94 0.94 0.96 0.96 0.9 0.9 0.9 0.88
Endoplasmic Reticulum 0 0 0 0 0.08 0.1 0.19 0.17 0.13 0.17 0.11 0.19 0 0 0 0 0.07 0 0 0 0.06 0.05 0.06 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0.09 0 0.07 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 3 8 6 7 17 7 0 0 0 0 1 0 0 0 0 0 0 0
Bud 0 0 0 1 0 0 1 0 0 0 0 1 0 0 0 0 1 1 0 1 0 3 0 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 6 2 4 3 14 13 17 14 20 22 12 2 6 1 5 4 9 0 0 0 0 0 0
Cytoplasm 219 459 182 118 100 261 311 212 207 171 140 139 147 311 230 392 390 336 201 438 155 187 172 208
Endoplasmic Reticulum 2 9 1 3 9 29 75 51 33 40 23 40 1 4 5 17 27 10 5 3 10 10 10 11
Endosome 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0
Golgi 0 2 0 0 0 2 5 26 5 17 17 16 1 1 1 4 6 8 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1 2 3 10 3 10 7 0 1 0 13 8 1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 0 1 7 1 1 3 8 0 1 1 7 11 14 0 6 3 2 3 2
Unique Cell Count 221 468 185 121 109 299 393 292 250 238 201 211 150 315 238 414 414 357 210 456 173 208 192 237
Labelled Cell Count 223 476 187 126 113 308 412 324 276 260 232 230 151 324 239 442 449 379 210 456 173 208 192 237


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 90.9 67.2 71.8 63.5 37.1 42.0 37.1 32.6 31.1 31.6 29.7 29.6 71.0 67.3 62.7 82.6 102.1 103.3 79.4 74.8 72.8
Std Deviation (1e-4) 15.5 12.4 16.5 13.7 12.7 7.9 7.1 7.2 6.5 6.4 6.6 6.3 19.6 15.3 16.0 33.6 34.9 33.7 22.0 23.4 25.0
Intensity Change (Log2) – – – -0.18 -0.95 -0.77 -0.95 -1.14 -1.21 -1.19 -1.27 -1.28 -0.02 -0.09 -0.2 0.2 0.51 0.52 0.15 0.06 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 4.0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2.2 0 2.6 2.5 3.4 4.0 2.5 0 0 0 0 0 0
Cytoplasm -0.5 -2.8 -4.3 -6.1 -7.2 -5.2 -7.3 -7.6 -8.2 -0.3 0.3 -1.1 -2.1 -2.3 -2.3
Endoplasmic Reticulum 0 0 4.1 6.2 5.8 4.9 5.6 4.4 6.0 0 0 0 2.4 3.2 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 4.2 0 3.7 4.0 3.8 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0