Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0.05 0 0.06 0.06 0.08 0.11 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.98 0.98 0.92 0.87 0.79 0.73 0.83 0.72 0.7 0.66 0.98 0.99 0.97 0.95 0.94 0.94 0.96 0.96 0.9 0.9 0.9 0.88
Endoplasmic Reticulum 0 0 0 0 0.08 0.1 0.19 0.17 0.13 0.17 0.11 0.19 0 0 0 0 0.07 0 0 0 0.06 0.05 0.06 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0.09 0 0.07 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 3 8 6 7 17 7 0 0 0 0 1 0 0 0 0 0 0 0
Bud 0 0 0 1 0 0 1 0 0 0 0 1 0 0 0 0 1 1 0 1 0 3 0 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 6 2 4 3 14 13 17 14 20 22 12 2 6 1 5 4 9 0 0 0 0 0 0
Cytoplasm 219 459 182 118 100 261 311 212 207 171 140 139 147 311 230 392 390 336 201 438 155 187 172 208
Endoplasmic Reticulum 2 9 1 3 9 29 75 51 33 40 23 40 1 4 5 17 27 10 5 3 10 10 10 11
Endosome 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0
Golgi 0 2 0 0 0 2 5 26 5 17 17 16 1 1 1 4 6 8 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1 2 3 10 3 10 7 0 1 0 13 8 1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 0 1 7 1 1 3 8 0 1 1 7 11 14 0 6 3 2 3 2
Unique Cell Count 221 468 185 121 109 299 393 292 250 238 201 211 150 315 238 414 414 357 210 456 173 208 192 237
Labelled Cell Count 223 476 187 126 113 308 412 324 276 260 232 230 151 324 239 442 449 379 210 456 173 208 192 237


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 90.9 67.2 71.8 63.5 37.1 42.0 37.1 32.6 31.1 31.6 29.7 29.6 71.0 67.3 62.7 82.6 102.1 103.3 79.4 74.8 72.8
Std Deviation (1e-4) 15.5 12.4 16.5 13.7 12.7 7.9 7.1 7.2 6.5 6.4 6.6 6.3 19.6 15.3 16.0 33.6 34.9 33.7 22.0 23.4 25.0
Intensity Change (Log2) -0.18 -0.95 -0.77 -0.95 -1.14 -1.21 -1.19 -1.27 -1.28 -0.02 -0.09 -0.2 0.2 0.51 0.52 0.15 0.06 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 4.0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2.2 0 2.6 2.5 3.4 4.0 2.5 0 0 0 0 0 0
Cytoplasm -0.5 -2.8 -4.3 -6.1 -7.2 -5.2 -7.3 -7.6 -8.2 -0.3 0.3 -1.1 -2.1 -2.3 -2.3
Endoplasmic Reticulum 0 0 4.1 6.2 5.8 4.9 5.6 4.4 6.0 0 0 0 2.4 3.2 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 4.2 0 3.7 4.0 3.8 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 64.8071 83.3438 76.9755 73.4064 55.2698 75.9189 87.8714 110.8106 100.2103 97.5834 87.2656 99.0783 78.0708 93.9676 87.1596 83.8963 71.5479 81.5266
Actin 0.0234 0.0003 0.0115 0.0012 0.0011 0.0065 0.0039 0.0002 0.0042 0.0003 0.0011 0.0009 0.0009 0.0009 0.0038 0.0095 0.0011 0.0071
Bud 0.0018 0.0004 0.0011 0.0016 0.0007 0.0013 0.0016 0.0005 0.0012 0.0012 0.0016 0.0009 0.0014 0.0004 0.001 0.0018 0.0015 0.001
Bud Neck 0.0016 0.0007 0.0018 0.0016 0.001 0.0014 0.0009 0.0006 0.0008 0.001 0.0012 0.0027 0.0008 0.0008 0.0009 0.0016 0.0007 0.0034
Bud Periphery 0.0028 0.0004 0.0011 0.0014 0.0004 0.0012 0.0026 0.0002 0.0015 0.0007 0.0018 0.0008 0.002 0.0004 0.0018 0.0013 0.0009 0.0016
Bud Site 0.0059 0.0003 0.0009 0.0004 0.0008 0.0004 0.0021 0.0002 0.0019 0.0003 0.0005 0.0002 0.0007 0.0027 0.0003 0.0019 0.0004 0.0005
Cell Periphery 0.0166 0.0129 0.0121 0.0116 0.0021 0.0033 0.0168 0.0085 0.0249 0.0192 0.0118 0.006 0.0181 0.012 0.0144 0.0258 0.0181 0.0023
Cytoplasm 0.5925 0.9047 0.7657 0.6212 0.6932 0.7746 0.6786 0.9172 0.7903 0.7124 0.7545 0.821 0.7101 0.8934 0.8055 0.6269 0.6604 0.7566
Cytoplasmic Foci 0.013 0.0012 0.0081 0.0033 0.0988 0.0206 0.0075 0.001 0.0068 0.0135 0.0046 0.0079 0.0092 0.0019 0.0036 0.0059 0.0219 0.0116
Eisosomes 0.0004 0 0.0001 0 0.0006 0.0003 0.0003 0 0.0002 0.0001 0.0001 0 0.0002 0.0001 0.0002 0.0001 0.0002 0.0002
Endoplasmic Reticulum 0.092 0.0244 0.0356 0.0033 0.0214 0.0247 0.0874 0.0187 0.0328 0.0211 0.037 0.0707 0.0703 0.0327 0.0396 0.0136 0.0058 0.0787
Endosome 0.0185 0.0035 0.0179 0.0117 0.0152 0.0297 0.0104 0.0026 0.0061 0.0123 0.0138 0.0184 0.012 0.0049 0.0083 0.014 0.0145 0.035
Golgi 0.0063 0.0008 0.0027 0.0012 0.0047 0.0092 0.0013 0.0003 0.0017 0.0005 0.001 0.0037 0.0015 0.0023 0.0065 0.0016 0.0012 0.0059
Lipid Particles 0.0076 0.001 0.0034 0.0013 0.0283 0.0207 0.0042 0.0007 0.0032 0.0053 0.0032 0.0018 0.0043 0.0013 0.0022 0.0023 0.0083 0.0073
Mitochondria 0.0159 0.0007 0.0026 0.0007 0.0158 0.0162 0.006 0.0003 0.0061 0.0018 0.01 0.0052 0.007 0.0046 0.0059 0.0013 0.0106 0.0255
None 0.0034 0.0002 0.0062 0.0003 0.0181 0.0047 0.0047 0.0002 0.0035 0.0026 0.004 0.0007 0.0036 0.0002 0.0007 0.0017 0.0586 0.0015
Nuclear Periphery 0.0308 0.0042 0.0082 0.0064 0.0141 0.0085 0.0234 0.0036 0.0073 0.0119 0.0135 0.0064 0.0182 0.0027 0.0058 0.0126 0.0063 0.0174
Nucleolus 0.0058 0.0001 0.0037 0.0004 0.0141 0.0018 0.0037 0.0001 0.005 0.0064 0.0023 0.0019 0.0035 0.0001 0.0002 0.0006 0.0076 0.0011
Nucleus 0.0105 0.0013 0.0047 0.0035 0.0049 0.002 0.0073 0.0014 0.0047 0.0054 0.0079 0.0025 0.0064 0.0013 0.0021 0.0042 0.0055 0.0047
Peroxisomes 0.0068 0 0.0006 0.0001 0.0023 0.0024 0.003 0 0.0022 0.0004 0.0004 0.0004 0.0004 0.0001 0.0046 0.001 0.0007 0.0011
Punctate Nuclear 0.0018 0 0.0007 0.0001 0.0179 0.0012 0.0012 0 0.0005 0.0029 0.0094 0.0003 0.0024 0.0001 0.0001 0.0056 0.0028 0.0021
Vacuole 0.12 0.0395 0.092 0.2408 0.0341 0.0546 0.1109 0.0407 0.0844 0.1467 0.0907 0.0395 0.1068 0.0342 0.0808 0.2236 0.1262 0.0275
Vacuole Periphery 0.0228 0.0034 0.0193 0.0879 0.0104 0.0149 0.0222 0.0032 0.0108 0.0338 0.0296 0.0083 0.02 0.0029 0.0115 0.0431 0.0467 0.0079

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2149.0549 2339.5865 2883.3751 2326.7146 2636.5826 2779.769 2037.9202 1993.4185 2222.9014 1978.0408
Translational Efficiency 1.1117 1.1419 0.9497 1.1054 1.2728 0.8791 0.9148 1.2138 0.9694 1.1829

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1070 962 1621 1254 1951 1983 1702 719 3021 2945 3323 1973

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2951.12 4730.96 6627.34 6132.95 4134.66 5059.04 5951.25 6465.51 3715.47 4951.87 6281.05 6254.14
Standard Deviation 653.94 1122.61 1435.50 1724.94 785.57 898.61 1285.52 1933.55 932.96 989.48 1402.09 1810.85
Intensity Change Log 2 0.680870 1.167167 1.055319 0.291095 0.525424 0.644995 0.466382 0.827972 0.830249

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000136 0.000166 0.000217 0.000348 0.000065 0.000069 0.000091 0.000890 0.000090 0.000101 0.000152 0.000546
Bud Neck 0.000366 0.000628 0.001930 0.002380 0.000457 0.000470 0.001563 0.002180 0.000424 0.000522 0.001742 0.002307
Bud Site 0.006094 0.005729 0.005118 0.012357 0.003166 0.003811 0.005346 0.022681 0.004203 0.004437 0.005235 0.016119
Cell Periphery 0.015523 0.030653 0.051794 0.041933 0.011078 0.009361 0.009722 0.057733 0.012652 0.016316 0.030246 0.047691
Cytoplasm 0.211332* 0.542246* 0.258363* 0.372100* 0.497506* 0.565536* 0.420466* 0.445730* 0.396147* 0.557928* 0.341390* 0.398932*
Cytoplasmic Foci 0.034010 0.013189 0.004238 0.006610 0.021153 0.013538 0.004279 0.011827 0.025707 0.013424 0.004259 0.008511
Eisosomes 0.000003 0.000003 0.000007 0.000007 0.000002 0.000001 0.000002 0.000020 0.000002 0.000002 0.000004 0.000012
Endoplasmic Reticulum 0.005896 0.002992 0.002377 0.000767 0.004758 0.001786 0.000827 0.003654 0.005161 0.002180 0.001583 0.001820
Endosome 0.002612 0.001659 0.001184 0.004600 0.002059 0.001700 0.001152 0.002737 0.002255 0.001687 0.001168 0.003921
Golgi 0.273988* 0.089178 0.042897 0.023278 0.134886* 0.073320 0.018824 0.049124 0.184154* 0.078500 0.030567 0.032697
Lipid Particles 0.011915 0.025411 0.039106 0.035144 0.015745 0.013845 0.012468 0.042039 0.014389 0.017623 0.025462 0.037657
Mitochondria 0.000718 0.000304 0.000230 0.001860 0.000262 0.000353 0.000276 0.000699 0.000423 0.000337 0.000254 0.001437
Mitotic Spindle 0.001707 0.001133 0.001001 0.002872 0.001597 0.001819 0.000623 0.001589 0.001636 0.001595 0.000807 0.002404
None 0.001880 0.002168 0.000657 0.002746 0.001599 0.000969 0.000818 0.008364 0.001698 0.001360 0.000740 0.004793
Nuclear Periphery 0.000605 0.000781 0.001820 0.000701 0.000865 0.000427 0.000460 0.000787 0.000773 0.000543 0.001124 0.000732
Nuclear Periphery Foci 0.010570 0.016063 0.014020 0.009914 0.020958 0.012307 0.007303 0.010845 0.017279 0.013534 0.010579 0.010253
Nucleolus 0.000320 0.000180 0.000132 0.000335 0.000055 0.000078 0.000207 0.000277 0.000149 0.000111 0.000171 0.000314
Nucleus 0.003141 0.004629 0.004940 0.006806 0.002013 0.002367 0.007251 0.008237 0.002413 0.003106 0.006124 0.007327
Peroxisomes 0.000069 0.000126 0.000040 0.000243 0.000022 0.000041 0.000063 0.000214 0.000039 0.000069 0.000052 0.000233
Vacuole 0.072789 0.152686 0.453544* 0.410933* 0.107682 0.170593 0.432344* 0.265960* 0.095323 0.164744 0.442685* 0.358102*
Vacuole Periphery 0.346327* 0.110078* 0.116385* 0.064064 0.174072* 0.127607* 0.075915 0.064414 0.235082* 0.121881* 0.095657 0.064192

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.25 -2.67 -3.85 -3.26 -1.99 -0.35 -4.57 -3.40 -3.38 -3.19 -0.25 -5.01 -4.88 -4.81 -3.91
Bud Neck -1.77 -8.23 -8.21 -7.67 -0.24 -0.09 -7.26 -4.89 -5.06 0.33 -0.86 -10.73 -9.29 -9.42 -0.12
Bud Site 0.53 0.22 -4.64 -4.93 -4.85 -1.65 -5.17 -7.07 -6.83 -5.79 -0.52 -3.85 -9.15 -9.01 -7.51
Cell Periphery -6.93 -15.22 -8.81 -2.04 4.00 2.46 2.59 -12.46 -12.96 -12.99 -4.04 -13.58 -15.65 -13.22 -6.91
Cytoplasm -23.07 -3.98 -13.31 10.46 -10.24 -5.81 7.17 3.21 7.49 -2.10 -17.31 6.76 -1.00 14.58 -7.04
Cytoplasmic Foci 8.21 12.80 10.66 3.64 -3.11 6.31 14.62 5.10 0.94 -4.31 10.31 19.38 12.44 4.05 -5.08
Eisosomes 0.32 -6.21 -4.07 -4.32 0.16 2.16 -2.65 -2.56 -2.62 -2.44 1.50 -7.86 -3.69 -3.84 -2.61
Endoplasmic Reticulum 3.56 4.75 6.91 6.18 6.61 6.72 9.74 1.51 -2.56 -4.14 7.33 9.49 7.58 1.36 -1.05
Endosome 3.88 9.49 -1.45 -2.47 -2.99 2.68 6.62 -1.93 -2.92 -4.55 4.73 11.30 -2.08 -3.00 -3.87
Golgi 23.69* 33.39* 36.43* 17.23 10.81 15.18 34.59 19.48 6.62 -9.27 26.47* 43.83 41.42 19.39 -1.03
Lipid Particles -6.70 -14.19 -9.08 -2.50 2.18 1.68 2.69 -8.93 -9.63 -10.02 -3.20 -9.61 -12.14 -9.92 -5.82
Mitochondria 2.08 2.26 -1.64 -2.70 -2.77 -1.06 -0.91 -3.07 -2.24 -2.70 1.11 1.80 -2.63 -3.00 -3.17
Mitotic Spindle 3.40 3.29 -1.57 -2.38 -2.42 -0.87 5.48 -0.34 0.31 -2.39 0.01 6.23 -1.68 -1.56 -3.12
None -0.57 4.62 -0.73 -0.41 -2.20 3.85 3.80 -2.68 -2.96 -3.01 1.86 6.31 -2.74 -3.08 -3.68
Nuclear Periphery -2.08 -11.09 -1.54 0.75 10.03 7.55 5.40 0.94 -6.18 -4.17 4.48 -6.46 0.39 -3.90 6.67
Nuclear Periphery Foci -5.06 -4.42 0.77 4.88 4.15 8.14 14.59 9.14 1.62 -4.09 4.56 9.25 8.22 4.27 0.97
Nucleolus 0.84 1.15 -0.78 -1.62 -1.91 -8.03 -16.76 -2.73 -2.43 -0.60 0.64 -0.68 -2.02 -2.46 -1.91
Nucleus -1.82 -4.56 -6.98 -5.09 -1.91 -1.02 -9.66 -5.49 -5.27 1.55 -1.97 -10.36 -9.91 -8.92 -0.21
Peroxisomes -0.72 1.19 -2.39 -1.41 -3.49 -3.60 -4.54 -4.34 -3.92 -3.46 -1.18 -0.52 -4.51 -3.69 -4.52
Vacuole -11.65 -50.10* -36.53 -24.30 4.59 -11.45 -41.18 -16.14 -9.01 13.84 -16.01 -63.07* -37.38 -25.03 10.71
Vacuole Periphery 26.51* 28.27* 35.11* 10.56 13.72 8.78 22.49 20.95 13.40 1.73 23.03 32.30 38.27 18.37 10.93