Standard name
Human Ortholog
Description Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of histidine triad (HIT) superfamily of nucleotide-binding proteins; protein abundance increases in response to DNA replication stress; human homolog HINT1 can complement yeast hnt1 mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.99 0.91 0.94 0.91 0.83 – 0.85 0.8 0.85 0.7 0.99 0.99 1.0 0.99 0.97 0.99 0.97 0.94 0.97 0.92 0.96 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 – 0 0.05 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.11 0.06 0.05 0.11 – 0.07 0.09 0.05 0.17 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 – 3 6 8 10 0 0 0 0 0 0 0 0 0 1 0 1
Bud 0 0 0 0 0 1 0 – 1 1 0 2 0 0 0 0 0 0 0 1 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 1 5 6 5 10 – 12 15 13 17 2 1 0 1 0 0 0 0 1 0 1 0
Cytoplasm 223 229 177 242 314 285 415 – 336 307 310 338 379 361 477 86 89 69 214 208 173 199 383 343
Endoplasmic Reticulum 0 1 0 4 6 8 23 – 17 18 14 33 2 3 0 3 2 2 0 0 0 4 6 4
Endosome 1 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 1 0 0 1 0 1
Golgi 0 0 0 0 0 0 3 – 3 3 2 9 0 0 0 0 0 0 0 0 0 0 1 0
Mitochondria 0 1 0 0 0 4 0 – 3 2 5 13 0 0 0 1 3 0 0 0 0 0 0 0
Nucleus 0 0 1 0 3 2 10 – 11 12 9 12 1 0 1 1 0 0 0 6 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 0 – 0 0 0 2 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 2 0 – 1 2 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 1 29 19 17 55 – 26 34 20 83 0 0 3 0 0 1 0 3 0 3 2 1
Unique Cell Count 224 231 178 266 335 313 503 395 382 364 485 381 363 479 87 92 70 221 223 179 217 401 362
Labelled Cell Count 224 234 180 280 348 326 517 413 400 381 520 384 365 481 92 95 72 221 223 179 217 401 362


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 31.0 26.3 24.0 24.4 25.4 17.6 22.3 – 20.2 20.4 19.0 19.7 31.8 31.0 31.5 35.7 35.2 37.7 25.9 28.8 29.6
Std Deviation (1e-4) 4.8 5.4 4.4 4.1 4.0 4.4 4.5 – 4.4 4.1 4.4 4.1 5.7 6.1 6.2 14.0 14.4 11.8 6.0 5.4 5.8
Intensity Change (Log2) – – – 0.02 0.08 -0.45 -0.11 – -0.25 -0.24 -0.34 -0.29 0.4 0.37 0.39 0.57 0.55 0.65 0.11 0.26 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 2.2 0 0 0 0 0 0 0 0
Cytoplasm -3.8 -3.0 -3.8 -5.8 – -5.2 -6.1 -5.2 -8.2 0.1 0 0.2 -0.5 -1.7 -0.7
Endoplasmic Reticulum 0 0 0 2.9 – 2.8 2.9 0 3.6 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 4.3 2.8 2.8 4.3 – 3.1 3.8 2.8 5.7 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 18.7087 25.4219 23.1099 21.906 21.9905 22.3308 23.3881 29.9791 28.3556 27.0594 25.2369 28.8156 28.5112 33.1717 30.9294 28.7092 25.3473 29.6843
Actin 0.0213 0.0002 0.015 0.0001 0.0001 0.0016 0.0386 0.0037 0.0085 0.0027 0.0192 0.0035 0.0113 0.0001 0.0008 0.0002 0.0004 0.0005
Bud 0.0003 0 0.0001 0 0 0.0001 0.0009 0.0002 0.0002 0.0031 0.0003 0.0001 0.0001 0 0.0003 0.0001 0.0003 0
Bud Neck 0.0004 0.0001 0.0003 0.0002 0.0004 0.0014 0.0037 0.0002 0.0004 0.0003 0.0008 0.0006 0.0004 0.0001 0.0002 0.0005 0.0006 0.0005
Bud Periphery 0.0003 0 0 0 0 0.0001 0.0007 0.0001 0.0003 0.0024 0.0007 0 0.0001 0 0.0002 0.0001 0.0001 0
Bud Site 0.0006 0 0.0003 0 0 0 0.0023 0.0002 0.0007 0.0014 0.0021 0.0002 0.0006 0 0.0002 0.0001 0.0001 0
Cell Periphery 0.0001 0 0 0 0 0 0.0002 0 0 0.0001 0.0002 0 0 0 0 0.0001 0 0
Cytoplasm 0.832 0.9915 0.9524 0.9827 0.9859 0.9451 0.8122 0.9752 0.942 0.906 0.8601 0.9522 0.9184 0.9934 0.9641 0.957 0.9175 0.9538
Cytoplasmic Foci 0.0148 0.0002 0.0029 0.0002 0.0004 0.0009 0.0149 0.0013 0.0034 0.0047 0.0052 0.0025 0.0044 0.0001 0.0014 0.0004 0.0232 0.003
Eisosomes 0.0004 0 0 0 0 0 0.0003 0 0.0001 0 0.0005 0.0001 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0036 0.0002 0.0006 0.0004 0.0007 0.0025 0.003 0.0005 0.0007 0.0013 0.0013 0.0013 0.0018 0.0001 0.0003 0.003 0.0009 0.0006
Endosome 0.0215 0 0.0007 0.0001 0.0002 0.0009 0.0137 0.0009 0.0014 0.008 0.0103 0.0009 0.0037 0 0.0026 0.0012 0.0166 0.0023
Golgi 0.0096 0 0.001 0 0 0.0001 0.0062 0.0015 0.0013 0.0017 0.01 0.0003 0.0011 0 0.0007 0 0.002 0.0012
Lipid Particles 0.0188 0 0.0007 0 0 0 0.0102 0.0036 0.0044 0.0001 0.0183 0.0009 0.0059 0 0.0023 0.0001 0.0146 0.0006
Mitochondria 0.0073 0.0001 0.0002 0 0.0001 0.0002 0.0065 0.0011 0.0027 0.0361 0.0276 0.0004 0.002 0 0.0043 0.0002 0.0005 0.0015
None 0.0044 0.0002 0.0005 0.0001 0.0003 0.0004 0.0028 0.0005 0.0007 0.0053 0.0023 0.0016 0.0021 0.0001 0.0008 0.0004 0.0033 0.0003
Nuclear Periphery 0.0064 0.0007 0.0022 0.0022 0.0018 0.0055 0.0095 0.0011 0.0022 0.0032 0.0084 0.0033 0.0047 0.0004 0.0049 0.0049 0.0014 0.0057
Nucleolus 0.0008 0 0 0 0 0.0001 0.004 0 0.0002 0.0003 0.0008 0.0002 0.0003 0 0.0001 0.0001 0.0001 0.0001
Nucleus 0.0345 0.0066 0.0193 0.0137 0.0095 0.0395 0.0478 0.0087 0.0244 0.0163 0.0136 0.0275 0.0376 0.0055 0.012 0.0293 0.0097 0.0288
Peroxisomes 0.0061 0 0.0032 0 0 0 0.0044 0.0006 0.0046 0.0007 0.0128 0.0002 0.0028 0 0.0027 0 0.0039 0.0002
Punctate Nuclear 0.013 0.0001 0.0003 0.0001 0.0002 0.0009 0.0149 0.0002 0.0007 0.0004 0.0033 0.0039 0.0013 0 0.0004 0.0005 0.0013 0.0004
Vacuole 0.0028 0.0001 0.0002 0.0002 0.0002 0.0005 0.002 0.0002 0.0008 0.0031 0.0009 0.0002 0.0008 0 0.0007 0.0017 0.0028 0.0003
Vacuole Periphery 0.001 0 0 0.0001 0.0001 0.0002 0.001 0.0001 0.0002 0.0028 0.001 0.0001 0.0003 0 0.0009 0.0003 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 191.997 190.2445 190.2721 202.7305 196.4467 150.2759 197.583 193.3298 181.5762 188.7776
Translational Efficiency 1.9818 1.9448 1.8803 1.6667 1.7001 2.3761 1.8547 1.7441 1.9108 1.7126

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
933 1322 254 1383 1413 1928 2096 1001 2346 3250 2350 2384

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1553.49 2361.11 2958.21 2654.52 1680.23 2149.47 2720.22 2904.32 1629.83 2235.56 2745.94 2759.41
Standard Deviation 514.26 405.88 449.53 543.22 287.77 369.10 443.74 586.19 398.62 398.29 450.47 575.03
Intensity Change Log 2 — 0.603952 0.929212 0.772938 — 0.355322 0.695065 0.789542 — 0.480118 0.812297 0.781589

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000492 0.000209 0.000569 0.000477 0.000299 0.000064 0.000076 0.000807 0.000375 0.000123 0.000130 0.000616
Bud Neck 0.001073 0.000571 0.005131 0.004800 0.000398 0.000313 0.000471 0.001910 0.000666 0.000418 0.000974 0.003587
Bud Site 0.006309 0.001043 0.003424 0.008171 0.002062 0.000846 0.000579 0.008418 0.003751 0.000926 0.000887 0.008275
Cell Periphery 0.000326 0.000199 0.001276 0.000247 0.000069 0.000035 0.000085 0.000260 0.000171 0.000102 0.000214 0.000252
Cytoplasm 0.836822* 0.689358* 0.401013* 0.631806* 0.803729* 0.759858* 0.582927* 0.666165* 0.816890* 0.731181* 0.563264* 0.646233*
Cytoplasmic Foci 0.019021 0.006562 0.027424 0.005319 0.005504 0.002121 0.001597 0.008309 0.010880 0.003927 0.004389 0.006574
Eisosomes 0.000003 0.000001 0.000006 0.000004 0.000001 0.000001 0.000001 0.000004 0.000002 0.000001 0.000001 0.000004
Endoplasmic Reticulum 0.000612 0.000169 0.000193 0.000565 0.000288 0.000111 0.000042 0.000082 0.000417 0.000135 0.000059 0.000362
Endosome 0.000383 0.000275 0.000412 0.004836 0.000161 0.000231 0.000447 0.001968 0.000249 0.000249 0.000444 0.003632
Golgi 0.001381 0.000897 0.000858 0.004678 0.000584 0.000231 0.000221 0.003958 0.000901 0.000502 0.000290 0.004376
Lipid Particles 0.000518 0.000319 0.002520 0.001196 0.000203 0.000168 0.000130 0.000590 0.000328 0.000229 0.000389 0.000941
Mitochondria 0.000174 0.000216 0.000515 0.003785 0.000127 0.000129 0.000144 0.000881 0.000146 0.000164 0.000184 0.002566
Mitotic Spindle 0.002944 0.001341 0.004235 0.036854 0.001542 0.002330 0.001217 0.014311 0.002099 0.001928 0.001544 0.027388
None 0.005958 0.015019 0.008749 0.014285 0.013295 0.017646 0.017520 0.015563 0.010377 0.016577 0.016572 0.014822
Nuclear Periphery 0.000068 0.000089 0.001125 0.000794 0.000071 0.000086 0.000104 0.001089 0.000070 0.000088 0.000215 0.000918
Nuclear Periphery Foci 0.002687 0.001452 0.002692 0.005265 0.001770 0.000574 0.000307 0.002766 0.002135 0.000931 0.000565 0.004216
Nucleolus 0.000135 0.000132 0.000188 0.001766 0.000076 0.000227 0.000079 0.000168 0.000100 0.000188 0.000091 0.001095
Nucleus 0.115696 0.278417* 0.511611* 0.252247* 0.165190* 0.212093* 0.390990* 0.261493* 0.145507* 0.239072* 0.404027* 0.256129*
Peroxisomes 0.000059 0.000020 0.000041 0.000450 0.000044 0.000052 0.000008 0.000778 0.000050 0.000039 0.000011 0.000588
Vacuole 0.004760 0.002946 0.027835 0.021152 0.003522 0.002766 0.002995 0.009603 0.004014 0.002840 0.005680 0.016303
Vacuole Periphery 0.000582 0.000764 0.000181 0.001304 0.001065 0.000116 0.000059 0.000875 0.000873 0.000379 0.000072 0.001124

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.31 -2.83 3.20 -0.19 4.19 1.70 1.09 -1.01 -3.19 -2.55 2.72 1.50 0.20 -3.54 -1.75
Bud Neck 2.17 -8.56 -7.72 -9.08 5.99 0.08 -6.65 -8.46 -9.14 -3.79 1.53 -9.16 -11.01 -12.09 -2.82
Bud Site 5.03 -0.80 1.09 -6.58 2.25 1.75 1.13 -3.28 -4.87 -4.48 4.84 3.05 -2.17 -7.95 -6.00
Cell Periphery 1.40 -10.79 0.64 -1.43 11.36 4.17 -7.57 -12.17 -13.52 -7.80 2.21 -7.09 -6.87 -12.03 1.65
Cytoplasm 20.03 39.83 30.55 11.97 -19.40 10.04 42.68 20.23 13.28 -12.23 20.82 53.48 36.73 19.96 -13.20
Cytoplasmic Foci 6.81 -7.12 7.44 0.26 11.11 5.78 2.70 -7.60 -11.45 -9.67 8.54 2.86 0.64 -9.66 -2.61
Eisosomes 2.27 -8.15 -2.47 -10.12 7.67 1.71 -1.06 -4.48 -6.88 -4.24 2.82 -2.66 -5.71 -12.62 -3.62
Endoplasmic Reticulum 4.03 2.39 3.93 -0.13 2.49 1.66 2.39 2.46 1.64 0.55 3.64 4.65 3.85 0.30 -1.41
Endosome 1.16 -2.75 -2.11 -2.42 -0.53 -0.60 -0.44 -1.54 -1.21 -0.89 0.15 -0.38 -2.79 -2.72 -1.85
Golgi 1.48 0.52 0.85 -0.09 0.66 2.14 2.41 -1.01 -1.67 -1.74 2.43 4.45 -0.36 -1.42 -2.01
Lipid Particles 1.14 -7.98 -5.54 -6.20 6.16 0.53 -4.59 -7.37 -7.21 -6.02 1.08 -7.42 -9.18 -9.42 -3.40
Mitochondria -0.64 -9.65 -1.16 -1.08 -0.25 -0.23 -2.89 -1.12 -1.08 -0.74 -0.68 -5.28 -1.39 -1.33 -1.10
Mitotic Spindle 1.95 -1.76 -4.18 -5.32 -2.49 -0.97 0.28 -3.10 -2.62 -3.17 0.53 0.48 -5.49 -5.70 -5.65
None -8.53 -0.58 -2.71 5.95 -2.68 -3.29 -1.90 2.50 7.02 5.87 -7.12 -5.14 1.26 9.54 7.40
Nuclear Periphery -3.75 -14.86 -10.46 -9.82 10.35 -1.91 -11.05 -2.49 -2.28 -1.62 -2.98 -16.19 -6.23 -5.77 -1.64
Nuclear Periphery Foci 2.03 -4.06 0.70 -1.39 5.06 4.91 5.44 -0.21 -3.08 -3.17 4.82 5.13 0.08 -3.88 -4.05
Nucleolus 0.06 -4.23 -1.59 -1.59 -1.07 -1.62 -3.22 -2.50 1.13 0.05 -1.48 -2.43 -1.77 -1.15 -1.52
Nucleus -24.32 -25.64 -21.34 3.02 14.64 -10.93 -42.42 -11.94 -3.56 22.65 -23.97 -53.19 -22.90 -1.68 28.19
Peroxisomes 3.61 -1.67 -0.91 -1.77 -0.50 -0.35 1.68 -1.00 -0.94 -1.15 0.52 2.62 -1.28 -1.39 -1.63
Vacuole 1.45 -11.34 -22.68 -23.07 -1.14 -2.03 -8.93 -14.69 -14.08 -10.88 -0.97 -13.00 -26.74 -26.49 -18.24
Vacuole Periphery -0.22 2.40 1.73 0.70 -0.21 1.69 1.79 1.35 -1.09 -1.50 1.26 2.32 1.63 0.23 -2.53
Standard name
Human Ortholog
Description Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of histidine triad (HIT) superfamily of nucleotide-binding proteins; protein abundance increases in response to DNA replication stress; human homolog HINT1 can complement yeast hnt1 mutant
Localization
Cell Percentages cytoplasm (93%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Hnt1

Hnt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hnt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available