Standard name
Description Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of histidine triad (HIT) superfamily of nucleotide-binding proteins; protein abundance increases in response to DNA replication stress; human homolog HINT1 can complement yeast hnt1 mutant

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.00 0.99 0.99 0.91 0.94 0.91 0.83 0.85 0.80 0.85 0.70 0.99 0.99 1.00 0.99 0.97 0.99 0.97 0.94 0.97 0.92 0.96 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0 0.05 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.11 0.06 0.05 0.11 0.07 0.09 0.05 0.17 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 3 6 8 10 0 0 0 0 0 0 0 0 0 1 0 1
Bud 0 0 0 0 0 1 0 1 1 0 2 0 0 0 0 0 0 0 1 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2 1 5 6 5 10 12 15 13 17 2 1 0 1 0 0 0 0 1 0 1 0
Cytoplasm 223 229 177 242 314 285 415 336 307 310 338 379 361 477 86 89 69 214 208 173 199 383 343
Endoplasmic Reticulum 0 1 0 4 6 8 23 17 18 14 33 2 3 0 3 2 2 0 0 0 4 6 4
Endosome 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 1 0 1
Golgi 0 0 0 0 0 0 3 3 3 2 9 0 0 0 0 0 0 0 0 0 0 1 0
Mitochondria 0 1 0 0 0 4 0 3 2 5 13 0 0 0 1 3 0 0 0 0 0 0 0
Nucleus 0 0 1 0 3 2 10 11 12 9 12 1 0 1 1 0 0 0 6 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 2 0 1 2 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 1 29 19 17 55 26 34 20 83 0 0 3 0 0 1 0 3 0 3 2 1
Unique Cell Count 224 231 178 266 335 313 503 395 382 364 485 381 363 479 87 92 70 221 223 179 217 401 362
Labelled Cell Count 224 234 180 280 348 326 517 413 400 381 520 384 365 481 92 95 72 221 223 179 217 401 362

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cytoplasm, Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 31.0 26.3 24.0 24.4 25.4 17.6 22.3 20.2 20.4 19.0 19.7 31.8 31.0 31.5 35.7 35.2 37.7 25.9 28.8 29.6
Std Deviation (1e-4) 4.8 5.4 4.4 4.1 4.0 4.4 4.5 4.4 4.1 4.4 4.1 5.7 6.1 6.2 14.0 14.4 11.8 6.0 5.4 5.8
Intensity Change (Log2) 0.02 0.08 -0.45 -0.11 -0.25 -0.24 -0.34 -0.29 0.4 0.37 0.39 0.57 0.55 0.65 0.11 0.26 0.3

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 2.2 0 0 0 0 0 0 0 0
Cytoplasm -8.3 -3.4 -5.6 -4.9 -5.8 -5.5 -4.4 -8.7 -5.2 -5.6 -5.3 -3.3 -2.2 -2.3
Endoplasmic Reticulum 0 0 0 2.9 2.8 2.9 0 3.6 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0